##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512912_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2050861
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.795953992006286	31.0	30.0	34.0	22.0	34.0
2	28.848884444143216	31.0	30.0	34.0	18.0	34.0
3	29.02247251276415	31.0	30.0	34.0	22.0	34.0
4	32.43219213783869	35.0	33.0	37.0	23.0	37.0
5	32.62432997653181	35.0	33.0	37.0	25.0	37.0
6	32.47576115592427	36.0	33.0	37.0	23.0	37.0
7	32.431204747664516	36.0	33.0	37.0	23.0	37.0
8	32.30760787786203	36.0	33.0	37.0	22.0	37.0
9	33.5617284642889	38.0	34.0	39.0	18.0	39.0
10	33.44890755638729	38.0	33.0	39.0	17.0	39.0
11	33.48624992137449	38.0	34.0	39.0	18.0	39.0
12	33.38359596286633	38.0	34.0	39.0	17.0	39.0
13	33.34509847327537	38.0	34.0	39.0	17.0	39.0
14	34.18937412140559	39.0	33.0	41.0	17.0	41.0
15	34.350466462622286	39.0	34.0	41.0	17.0	41.0
16	34.288516384094294	39.0	34.0	41.0	17.0	41.0
17	34.050234998861455	39.0	33.0	41.0	16.0	41.0
18	34.05955352410524	39.0	33.0	41.0	16.0	41.0
19	34.17999805935166	39.0	34.0	41.0	16.0	41.0
20	34.149999926859984	39.0	34.0	41.0	13.0	41.0
21	34.06003868619082	39.0	34.0	41.0	10.0	41.0
22	34.06636627250701	39.0	34.0	41.0	10.0	41.0
23	33.73537455732007	39.0	33.0	41.0	2.0	41.0
24	33.791479286016944	39.0	34.0	41.0	2.0	41.0
25	33.512778291654094	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.730867879019943
1101	2	0.3832254597235014
1101	3	0.7392585201383568
1101	4	0.8453192574644532
1101	5	0.744315995325266
1101	6	0.7536346157568161
1101	7	0.5621496722257149
1101	8	0.8949959593449179
1101	9	0.9801239898997167
1101	10	1.0049864718482269
1101	11	0.9987188406151972
1101	12	0.7509807860793458
1101	13	1.2216882017411805
1101	14	1.3419452298727066
1101	15	1.1905428603220614
1101	16	0.9193272385630848
1101	17	0.2756242383735099
1101	18	0.6634524593816735
1101	19	0.253115229696526
1101	20	-0.37946061177895984
1101	21	0.6423010165872469
1101	22	0.6978557665278444
1101	23	0.2835418714620346
1101	24	0.7436851693363451
1101	25	0.4658570031599254
1102	1	1.021372207515391
1102	2	0.9375402920673892
1102	3	0.917097029653906
1102	4	0.9234829734344032
1102	5	0.928603550700835
1102	6	0.9810769568493924
1102	7	0.869607838719439
1102	8	0.9180154147000081
1102	9	1.2248772355019781
1102	10	1.2187185256915214
1102	11	1.1441074820227684
1102	12	1.1697721061935198
1102	13	1.0641928412465873
1102	14	1.3632099202721406
1102	15	1.171924831837984
1102	16	1.1830952760122955
1102	17	1.2878075816156667
1102	18	1.370873329298682
1102	19	1.1992187016426428
1102	20	1.3625005193617952
1102	21	1.4285284452857496
1102	22	1.2529325322376437
1102	23	1.1889583273353423
1102	24	0.6550844638607245
1102	25	1.1425007092497665
1103	1	0.49198291829187113
1103	2	0.5792554596633828
1103	3	0.701678401706598
1103	4	0.7377327918177201
1103	5	0.5363276752605586
1103	6	0.16354966049046737
1103	7	0.16954584370058257
1103	8	0.2588713289410336
1103	9	0.3813042519258758
1103	10	0.46713454099160145
1103	11	0.29019581175967346
1103	12	0.39163528390499636
1103	13	0.5057876690907293
1103	14	0.5735295177593045
1103	15	0.4335123950148585
1103	16	0.33358160180210206
1103	17	0.058835920998902225
1103	18	0.4604381793198016
1103	19	0.4017931106254622
1103	20	0.5745154053472064
1103	21	0.34682547323497204
1103	22	0.3337854664092248
1103	23	-0.2234533626752011
1103	24	-0.035520298745026935
1103	25	0.216630534957595
1104	1	0.8960287228643473
1104	2	0.7756121962427578
1104	3	0.7686952969084686
1104	4	0.9233636686747104
1104	5	0.7767866076398562
1104	6	0.6435542710939686
1104	7	0.932372852970552
1104	8	0.672707572717826
1104	9	1.1197393953644124
1104	10	1.0125405622624442
1104	11	0.7938545916343855
1104	12	1.0061311986442973
1104	13	1.0913919463121289
1104	14	1.254844603742626
1104	15	0.9958384444813149
1104	16	0.9625379650999264
1104	17	1.2793229603264606
1104	18	1.1092644703996157
1104	19	0.605501986480526
1104	20	0.7460653217800228
1104	21	0.9111837348601171
1104	22	0.6712337633675531
1104	23	1.1520696526594918
1104	24	1.0989178061196014
1104	25	0.521469451997767
1105	1	0.6148751346685053
1105	2	0.668492791360304
1105	3	0.7954343806483806
1105	4	0.8871823103723884
1105	5	0.6936674131702034
1105	6	0.8209506756651876
1105	7	0.8092796094133519
1105	8	0.8702667841958487
1105	9	0.5028730557143106
1105	10	0.636180440749051
1105	11	0.7618358999636641
1105	12	1.0034089829974775
1105	13	0.8637121419960394
1105	14	1.0418156675985273
1105	15	0.9717207535864887
1105	16	1.0341826480122123
1105	17	1.3457678168293157
1105	18	1.1417329194380699
1105	19	0.8340059076107948
1105	20	1.0009716661644106
1105	21	0.8953001983469235
1105	22	0.9463888332202259
1105	23	1.1340293849091978
1105	24	1.1767608995385004
1105	25	1.3818719541566864
1106	1	0.7559087854621573
1106	2	0.7271539802803169
1106	3	0.712661744482805
1106	4	0.8002978783773713
1106	5	0.5980903948073006
1106	6	0.8083502648341891
1106	7	0.7072155446763446
1106	8	0.7034801548774539
1106	9	0.9926177678213719
1106	10	0.6023566498993773
1106	11	0.5465181284106038
1106	12	0.8324823723532191
1106	13	0.7139246536673696
1106	14	0.9992061686878557
1106	15	0.7527861753107317
1106	16	0.888269420498986
1106	17	1.0548587349743528
1106	18	0.9196737348753601
1106	19	0.952011908233267
1106	20	1.0216744610601438
1106	21	1.0319369116989279
1106	22	0.9401559542908728
1106	23	1.1786907481211486
1106	24	0.8873677591712408
1106	25	1.015280531809971
1107	1	0.9224574156405652
1107	2	0.9554401611088608
1107	3	0.9312498061701326
1107	4	1.098546296883569
1107	5	1.0405546947770645
1107	6	1.0806936818284427
1107	7	0.8201220339415372
1107	8	0.9965470312004001
1107	9	0.8686381367357967
1107	10	1.1555461751488494
1107	11	1.1037675992300748
1107	12	1.2218082870795541
1107	13	1.0328302478615043
1107	14	1.3751325309497204
1107	15	1.132536764969032
1107	16	1.1789101220411666
1107	17	1.4356341568068274
1107	18	1.3404569946274023
1107	19	1.2517827216593318
1107	20	1.4502547090190205
1107	21	1.1882427357086058
1107	22	1.2602234958693472
1107	23	1.526092792269921
1107	24	1.3297464226124305
1107	25	1.5275004773799736
1108	1	0.7775373186331862
1108	2	0.8004331038347914
1108	3	0.807902937422547
1108	4	0.8979390713171114
1108	5	0.6944230545760206
1108	6	0.8580449209510448
1108	7	0.7014542786994724
1108	8	0.6921495644940094
1108	9	0.8198098798116362
1108	10	0.956050040039365
1108	11	0.6357305447222288
1108	12	0.7450077527549226
1108	13	0.8686168021761986
1108	14	0.9007153105016101
1108	15	0.9064592124379374
1108	16	0.6356408142908307
1108	17	0.9421914677801979
1108	18	1.00503816579441
1108	19	0.8694105205278504
1108	20	0.866953292466107
1108	21	0.7915672464524306
1108	22	0.795610999939079
1108	23	1.1509594273664305
1108	24	1.03705598802091
1108	25	1.1627940071599383
1109	1	0.6002264836562183
1109	2	0.6211283910547287
1109	3	0.8536817383609794
1109	4	0.784397394753249
1109	5	0.7072280256612942
1109	6	0.7126998454892188
1109	7	0.6134275312083304
1109	8	0.5407917434908569
1109	9	0.6018434060215583
1109	10	0.6045270080260963
1109	11	0.7012607056986297
1109	12	0.629337223898979
1109	13	0.6946546199973369
1109	14	0.7780010508427324
1109	15	0.736285165209722
1109	16	0.34324732071324604
1109	17	0.7127326009063992
1109	18	0.8096882558426088
1109	19	0.7884626214258006
1109	20	0.6156877086879362
1109	21	0.5762785584696672
1109	22	0.7414183234368821
1109	23	0.9966201512432775
1109	24	0.8777483652318523
1109	25	0.41981440386384605
1110	1	0.6893514020581613
1110	2	0.6761322964523337
1110	3	0.6894109631381653
1110	4	0.5934991866753663
1110	5	0.5093623027418843
1110	6	0.560848290273313
1110	7	0.5566350964631397
1110	8	0.5544304153256618
1110	9	0.6741492517704941
1110	10	0.5527986123463791
1110	11	0.5203715505789859
1110	12	0.5799774662357109
1110	13	0.6738122369502335
1110	14	0.7153495000022119
1110	15	0.5189482588964367
1110	16	0.6673599111107364
1110	17	0.8048149796745321
1110	18	0.24494588508735404
1110	19	0.6275524533574313
1110	20	0.7015217293823781
1110	21	0.8084241800211629
1110	22	0.8714463722194026
1110	23	0.9679542732574262
1110	24	0.7610881225873527
1110	25	0.8866460557348645
1111	1	0.279359080564074
1111	2	0.17354229789516395
1111	3	0.3406786352646698
1111	4	0.22034056775978428
1111	5	0.40928328802299774
1111	6	0.2881418316123998
1111	7	0.07350339377887138
1111	8	0.22974561274176608
1111	9	0.3947077465253557
1111	10	0.386124267788027
1111	11	0.16507121633271993
1111	12	-0.15765672104501505
1111	13	0.23547402999837175
1111	14	0.14257570930239893
1111	15	0.21581118291645396
1111	16	0.329638693392198
1111	17	-0.17521454765418554
1111	18	0.15228822833444156
1111	19	0.2746505696266368
1111	20	0.18884150699869195
1111	21	0.36632503460735677
1111	22	-0.059207063658625714
1111	23	0.36184163342689857
1111	24	0.3608267917237242
1111	25	0.5346786942681376
1112	1	0.17217408046972338
1112	2	0.08811494851027035
1112	3	0.006523408628037686
1112	4	0.10972141846814054
1112	5	0.05544155970896725
1112	6	-0.12021760203419518
1112	7	-0.17734046137509551
1112	8	0.017863937025708765
1112	9	-0.12630313989421182
1112	10	-0.09435064208246757
1112	11	-0.18366038898728476
1112	12	-0.23263351266947296
1112	13	-0.14701573967604986
1112	14	-0.19492538955722694
1112	15	-0.30565618474977896
1112	16	-0.13402649478089756
1112	17	0.07824052014832716
1112	18	-0.23090490355127002
1112	19	-0.05269030054131463
1112	20	-0.2919318324345568
1112	21	-0.18987053156884315
1112	22	-0.3556605661327268
1112	23	-0.11402501587279801
1112	24	0.01752739608500775
1112	25	-0.06343462704025882
1113	1	-0.3778711466994231
1113	2	-0.2907252673299219
1113	3	-0.22664837577621455
1113	4	-0.22989007105217496
1113	5	-0.46501581549180315
1113	6	-0.6363514557948626
1113	7	-0.6493918435444392
1113	8	-0.5561893363343877
1113	9	-0.506320055182016
1113	10	-0.3526522184324108
1113	11	-0.4353579122924529
1113	12	-0.5561025181802393
1113	13	-0.43263741848804216
1113	14	-0.16983375730659844
1113	15	-0.6572524309180707
1113	16	-0.6555569131427035
1113	17	-0.5224142591668723
1113	18	-0.6673241806349708
1113	19	-0.958845347394238
1113	20	-0.7038151719956645
1113	21	-0.5253740226211434
1113	22	-0.8849810669857234
1113	23	-4.594431225360196
1113	24	-2.0992650622903675
1113	25	-1.3039200108974
1114	1	-0.4170389463312354
1114	2	-0.6343685092929903
1114	3	-0.5342524935666013
1114	4	-0.4068406779267697
1114	5	-0.3825754248911224
1114	6	-1.0765473675046024
1114	7	-0.8976472849675439
1114	8	-0.7609199221196619
1114	9	-0.9615470829967236
1114	10	-0.5792479185959678
1114	11	-0.8212252145473755
1114	12	-1.7353184617821675
1114	13	-0.836320838793327
1114	14	-2.6967107770933474
1114	15	-1.4477107127226319
1114	16	-1.4977899956062473
1114	17	-1.198843568522598
1114	18	-1.1713659058047838
1114	19	-1.2265352589146303
1114	20	-0.731657178336711
1114	21	-0.7344756131647188
1114	22	-0.7552395670437448
1114	23	-4.890971311551613
1114	24	-2.2024861259747297
1114	25	-2.168954258052075
1115	1	-1.5102303845347684
1115	2	-1.3684963117578022
1115	3	-1.1674160667683502
1115	4	-1.184511811140002
1115	5	-1.1760363312916837
1115	6	-1.2541721083597892
1115	7	-1.719246478071888
1115	8	-2.0264427962445914
1115	9	-3.3685536308875115
1115	10	-3.6571013765377565
1115	11	-2.572112059108612
1115	12	-2.842167735239773
1115	13	-2.3263940675130783
1115	14	-3.007934956427423
1115	15	-2.7832787493741726
1115	16	-2.864403435326409
1115	17	-2.7718731186991796
1115	18	-5.558245428973397
1115	19	-3.3856750514962144
1115	20	-3.619839366176251
1115	21	-3.3844401433272004
1115	22	-2.9418712698099547
1115	23	-3.2005941458229046
1115	24	-2.9127573684033017
1115	25	-3.208464491245671
1116	1	-1.569240059912591
1116	2	-1.3720227921707284
1116	3	-1.7267779854294183
1116	4	-1.4733387455253997
1116	5	-1.611643892836181
1116	6	-4.120026885739751
1116	7	-2.518614246005626
1116	8	-2.8879788052846003
1116	9	-3.678275628532443
1116	10	-3.1755811758940062
1116	11	-2.56564122337873
1116	12	-2.487999464243675
1116	13	-3.720021058689145
1116	14	-6.763843230366838
1116	15	-4.749900117734509
1116	16	-3.5768552250108065
1116	17	-4.671583830859543
1116	18	-3.231136582477781
1116	19	-3.0869667278234516
1116	20	-3.739760353254507
1116	21	-4.706456822264606
1116	22	-4.2725929451014295
1116	23	-3.3267647008997834
1116	24	-4.323518182759884
1116	25	-4.082556821329799
1201	1	0.7355673912923208
1201	2	0.9159940309774015
1201	3	1.1838904390328686
1201	4	1.0834956477056323
1201	5	0.5806948054536889
1201	6	1.091343116545211
1201	7	1.1636509312416194
1201	8	1.2009685198964632
1201	9	1.7394526474157956
1201	10	1.4950176384925342
1201	11	1.7413825137084444
1201	12	1.4660331961808453
1201	13	1.6057010795228877
1201	14	1.6467967390300586
1201	15	1.76476386859337
1201	16	1.731257506684031
1201	17	1.9300762761657353
1201	18	1.7320900593367057
1201	19	1.890917053987387
1201	20	1.8203585675240248
1201	21	1.9503915631564723
1201	22	1.7209299973833936
1201	23	2.143330950909487
1201	24	1.9329678605654124
1201	25	2.1047947523422934
1202	1	1.1240203993762918
1202	2	1.0047757916247235
1202	3	1.0829880873220397
1202	4	1.258734030053958
1202	5	1.2563689031119125
1202	6	1.3126949342168217
1202	7	1.4792929122727116
1202	8	1.449144235103084
1202	9	1.758235969012226
1202	10	1.5749379347630565
1202	11	1.4663275925284296
1202	12	1.683435177116614
1202	13	1.681317445895516
1202	14	2.0870088136267455
1202	15	1.8917951380183453
1202	16	1.4014672895055682
1202	17	1.9327240311197613
1202	18	1.8890742738913389
1202	19	1.862740532488985
1202	20	1.5838372438868475
1202	21	1.756236404021088
1202	22	1.7156243591796212
1202	23	1.881410046146847
1202	24	1.9592465181809615
1202	25	2.1823578367300556
1203	1	1.2170555102985539
1203	2	1.2271816524523338
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2211	17	0.3657918358460819
2211	18	-0.08301000111467971
2211	19	0.11294132292815817
2211	20	0.0016215594412187784
2211	21	0.1953857784486175
2211	22	0.15122624071677393
2211	23	0.4375204367886454
2211	24	0.10138887996825474
2211	25	0.4129535579618988
2212	1	-0.02444315483635151
2212	2	0.0057519902305642745
2212	3	-0.29544293213411166
2212	4	0.09527728136244207
2212	5	-0.3236711972324997
2212	6	-0.29151918790213216
2212	7	-0.09885072398036954
2212	8	-0.06763735756035771
2212	9	-0.019509197352753915
2212	10	-0.31533648442898254
2212	11	-0.40562366263417715
2212	12	0.06188237235321736
2212	13	0.005687837746975788
2212	14	0.03794815873760626
2212	15	-0.16468347643056092
2212	16	-0.3145992362174326
2212	17	-0.08860763318484999
2212	18	0.1982076652236202
2212	19	-0.021349086791609295
2212	20	0.06183535658253447
2212	21	0.3058646728929588
2212	22	0.1746807304102802
2212	23	0.2166040814544843
2212	24	0.17902606762895346
2212	25	0.29620580544180797
2213	1	-0.25132750625601474
2213	2	-0.12926283059730892
2213	3	-0.29274166713647887
2213	4	-0.08527660864364606
2213	5	-0.1054160576030796
2213	6	-0.01538748547483948
2213	7	-0.2769981512445483
2213	8	-0.06493032719918546
2213	9	-0.5239677451576128
2213	10	-0.05559993848133615
2213	11	-0.19589472696640797
2213	12	-0.2188654644823842
2213	13	-0.2559151485575981
2213	14	-0.26608213786344237
2213	15	-0.35929985682771104
2213	16	-0.402230455891619
2213	17	-0.30203639481550937
2213	18	-0.3746119511567798
2213	19	-0.46934166407822886
2213	20	-0.08864870334034691
2213	21	-0.18724687074853819
2213	22	-0.4728226365620287
2213	23	-0.40450737301605955
2213	24	-0.16465340275706097
2213	25	0.05455904849723936
2214	1	-2.879940816559319
2214	2	-1.4469422926192408
2214	3	-1.692111673078788
2214	4	-3.428670611831098
2214	5	-1.8641589039924433
2214	6	-1.512272653674973
2214	7	-1.443856192531488
2214	8	-1.2138470466386586
2214	9	-0.9571056434230982
2214	10	-0.9382893134613326
2214	11	-1.130578523516121
2214	12	-1.0639072675709755
2214	13	-1.4062299894153831
2214	14	-1.370104557780877
2214	15	-1.7330831866602097
2214	16	-1.5436148498737268
2214	17	-3.604040772290336
2214	18	-3.484322121094319
2214	19	-2.2660553564540358
2214	20	-1.954457743614526
2214	21	-1.869665210853185
2214	22	-3.054535239644693
2214	23	-1.9859253984360201
2214	24	-3.073226959716944
2214	25	-2.7652527341407556
2215	1	-2.154535738863842
2215	2	-3.7533894346561567
2215	3	-2.431263288055156
2215	4	-3.845875434296943
2215	5	-2.5599896051926976
2215	6	-2.331600004766063
2215	7	-2.6693548426803346
2215	8	-1.776587474499678
2215	9	-4.272433065770198
2215	10	-2.739204131011686
2215	11	-2.263440346953516
2215	12	-2.24804170573789
2215	13	-2.322806427832475
2215	14	-2.173568148014965
2215	15	-2.208680450037889
2215	16	-2.2224598016888066
2215	17	-2.71820494026581
2215	18	-2.193178871260372
2215	19	-2.377697766387122
2215	20	-2.208890249197058
2215	21	-2.778157615725924
2215	22	-2.5383116750862555
2215	23	-2.5651914458670753
2215	24	-2.4980674314082414
2215	25	-3.350287190532761
2216	1	-3.4553788636385043
2216	2	-3.4699868212343574
2216	3	-4.827422117934361
2216	4	-5.211510313481263
2216	5	-4.896688879411695
2216	6	-3.8507716789966757
2216	7	-5.751457248002733
2216	8	-3.5997881568928243
2216	9	-4.1317984139394355
2216	10	-3.288669566572697
2216	11	-3.648367324413634
2216	12	-3.9862006191267447
2216	13	-3.51149863443613
2216	14	-4.445270902943577
2216	15	-3.932061284455756
2216	16	-4.18859722513055
2216	17	-3.906060974713249
2216	18	-3.560213270489111
2216	19	-4.2706377099428146
2216	20	-4.830568845976774
2216	21	-4.652981636739064
2216	22	-4.154822549969143
2216	23	-4.1636091824563195
2216	24	-4.801837012049962
2216	25	-4.685751617132915
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106197.0
3	3.0
4	882.0
5	5825.0
6	8052.0
7	7405.0
8	6638.0
9	5892.0
10	5122.0
11	4769.0
12	4464.0
13	4242.0
14	4186.0
15	4321.0
16	4326.0
17	4585.0
18	5271.0
19	6401.0
20	8330.0
21	10741.0
22	13462.0
23	16237.0
24	18891.0
25	20854.0
26	23197.0
27	25254.0
28	28370.0
29	33271.0
30	40057.0
31	49179.0
32	61770.0
33	77592.0
34	100988.0
35	131411.0
36	177375.0
37	271882.0
38	753419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.441344472032974	17.997627114497842	12.47242437956383	24.08860403390535
2	15.713164118694072	20.054080413527416	40.21422475800356	24.01853070977495
3	19.461238446436425	25.42720267464326	28.7094046519521	26.402154226968218
4	12.725455104041197	16.260905185235757	37.20793122113692	33.80570848958613
5	13.625522099467721	38.412817618129196	33.879640985753056	14.082019296650031
6	32.16187319107031	35.71792823474814	18.06199367658448	14.058204897597074
7	28.738292559501588	31.853119536060113	21.218544907821517	18.19004299661678
8	26.506768609894365	34.96501721561051	20.316324030297913	18.211890144197213
9	27.887889763733416	14.754192763158535	19.443801894075815	37.914115579032234
10	16.83658778227322	28.213363657216483	32.23866392710555	22.71138463340475
11	34.79998243105409	22.771891638645826	23.431166333995424	18.996959596304666
12	24.28775443268296	24.86699998828624	30.022324479030416	20.822921100000393
13	29.49836053253534	21.800988804577138	25.264879283563207	23.435771379324315
14	22.384704657103175	21.912532783613024	26.953571473888772	28.749191085395026
15	24.407474464523624	29.429996437477733	23.32070996676606	22.841819131232587
16	23.05853209316762	27.683337066714298	26.359036075196634	22.899094764921447
17	22.38999236151276	27.84113898874238	26.66695951601764	23.101909133727215
18	22.29198697767951	26.613269295535414	28.654522913524566	22.44022081326051
19	23.83141923269708	26.35387377819599	27.612245884045084	22.202461105061843
20	24.56188385914103	25.935567753714967	27.64039800033481	21.862150386809198
21	24.365697498511597	25.735806517729042	27.025395524063285	22.873100459696076
22	24.383339125409513	26.490499693475517	26.913142285930718	22.21301889518425
23	23.541214194726027	26.469230281171146	27.355692971453394	22.633862552649433
24	23.684735814902787	25.933443598557147	27.73193154315839	22.64988904338167
25	23.490679022407633	26.32425816262688	27.593197575052443	22.59186523991305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	414.0
1	414.0
2	952.0
3	1490.0
4	1490.0
5	1490.0
6	2758.5
7	4027.0
8	4027.0
9	4027.0
10	4621.5
11	5216.0
12	5216.0
13	5216.0
14	6680.5
15	8145.0
16	8145.0
17	8145.0
18	14663.5
19	21182.0
20	21182.0
21	21182.0
22	32354.5
23	43527.0
24	43527.0
25	43527.0
26	64351.5
27	85176.0
28	85176.0
29	85176.0
30	107932.0
31	130688.0
32	130688.0
33	130688.0
34	149040.0
35	167392.0
36	167392.0
37	167392.0
38	182128.0
39	196864.0
40	196864.0
41	196864.0
42	214385.0
43	231906.0
44	231906.0
45	231906.0
46	253816.0
47	275726.0
48	275726.0
49	275726.0
50	272372.0
51	269018.0
52	269018.0
53	269018.0
54	243850.5
55	218683.0
56	218683.0
57	218683.0
58	190904.0
59	163125.0
60	163125.0
61	163125.0
62	138283.5
63	113442.0
64	113442.0
65	113442.0
66	88260.0
67	63078.0
68	63078.0
69	63078.0
70	47173.0
71	31268.0
72	31268.0
73	31268.0
74	22314.5
75	13361.0
76	13361.0
77	13361.0
78	9162.5
79	4964.0
80	4964.0
81	4964.0
82	3296.5
83	1629.0
84	1629.0
85	1629.0
86	1022.0
87	415.0
88	415.0
89	415.0
90	255.5
91	96.0
92	96.0
93	96.0
94	60.5
95	25.0
96	25.0
97	25.0
98	14.5
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33819941965837763
2	0.010288361814867024
3	0.009508201677246775
4	0.011263561986892334
5	0.011994962115911317
6	0.08055153420929063
7	0.0794788140200628
8	0.10429765839810694
9	0.08459861492319568
10	0.09122997609296779
11	0.08732917540486654
12	0.0969836571079171
13	0.08386721479417669
14	0.08869445564570197
15	0.08537877506081593
16	0.08679281531025262
17	0.09878777742616394
18	0.1009819778132209
19	0.09478945672086016
20	0.09352169649722726
21	0.08196557445872733
22	0.1016646179336386
23	0.09415557660904371
24	0.0907911360155564
25	0.0890845357145121
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2050861.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.42657358018062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32916596933019	38.44396915264367
2	14.142461870393932	14.828816357062127
3	5.286047199941716	8.313880274281564
4	2.486822255539299	5.215022798434473
5	1.3936819717156985	3.6532985218762137
6	0.8809294179975499	2.7710466570956545
7	0.5262805752441827	1.9313761111301249
8	0.3862058696130808	1.619796035229426
9	0.2661891765376463	1.2559847804998743
>10	1.1857206546205241	10.597051687133666
>50	0.06844677416263335	2.496130965392186
>100	0.041668606000502595	4.259061096475756
>500	0.003752740531233294	1.4287500449442616
>1k	0.0023454628320208055	2.0295057957343046
>5k	1.8763702656166443E-4	0.6191020660876276
>10k+	9.381851328083221E-5	0.5372076559791108
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10922	0.532556813943022	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6302	0.30728557420517527	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6285	0.3064566540589538	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3397	0.16563774921849897	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3011	0.14681638589841048	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2369	0.11551246037639802	No Hit
GTCCTACAGTGGACATTTCTAAATT	2251	0.10975877936144868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.752001720253104E-5
2	0.0	0.0	0.0	0.0	1.4628002580379656E-4
3	0.0	0.0	0.0	0.0	1.4628002580379656E-4
4	0.0	0.0	0.0	0.0	1.4628002580379656E-4
5	0.0	0.0	0.0	0.0	1.4628002580379656E-4
6	0.0	0.0	0.0	0.0	1.4628002580379656E-4
7	0.0	0.0	0.0	0.0	1.4628002580379656E-4
8	0.0	0.0	0.0	0.0	1.4628002580379656E-4
9	0.0	0.0	0.0	0.0	1.4628002580379656E-4
10	0.0	0.0	0.0	0.0	1.4628002580379656E-4
11	0.0	0.0	0.0	0.0	1.4628002580379656E-4
12	0.0	0.0	0.0	0.0	1.4628002580379656E-4
13	0.0	0.0	0.0	4.876000860126552E-5	1.4628002580379656E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGT	25	0.0060365694	18.997902	4
CCGTATG	70	7.279041E-6	13.568936	9
CGACAGT	50	0.0015010807	13.298532	6
TACCGTA	65	5.4597942E-5	13.152394	7
ACCGTAT	80	2.002771E-6	13.061057	8
GTCTTAT	165	0.0	12.699668	1
TAGGACC	525	0.0	12.122471	4
GTAATAC	160	3.6379788E-12	11.873688	3
GGTATCA	2260	0.0	11.589852	1
TTAGACT	100	1.9329418E-6	11.398741	4
TAGGACT	220	0.0	11.226032	4
CCGATTT	60	0.0058837137	11.081568	13
TAATACA	225	0.0	10.976565	4
CTAATAC	140	8.36917E-9	10.855944	3
CAACGTC	70	0.0014937365	10.855414	15
CCCCGTA	250	0.0	10.638306	15
GTAGGAC	1350	0.0	10.624753	3
TGGCGAG	1215	0.0	10.553875	18
TAGGCCC	100	2.4023226E-5	10.448846	4
CTAGGAC	200	1.8189894E-12	10.448846	3
>>END_MODULE