FastQCFastQC Report
Thu 26 May 2016
SRR1512912_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512912_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050861
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT137450.6702063182243945No Hit
TATCAACGCAGAGTACTTTTTTTTT103010.5022768486016361No Hit
GGTATCAACGCAGAGTACTTTTTTT79430.387300748319852No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48530.23663232174194154No Hit
GTACTGGTTCACTATCGGTCAGTCA29590.14428086545114466No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28940.14111146489206242No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23890.11648766054842333No Hit
GTCCTACAGTGGACATTTCTAAATT21850.10654061879376517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTA400.005306504714.23919313
TACACCG655.405768E-513.1663415
TAGCACG604.0603618E-412.6786994
GGTATCA22800.012.6434771
CCAACGA853.9786482E-612.28479419
CCGTGCG550.00307469112.0879299
TAGGACC4700.011.7345414
CCGAATG1001.9475028E-611.39163218
TTAGGAC8100.011.1555563
GACGTGA6650.010.8637427
ATTAGCC803.7295584E-410.7005273
TAGGCTG2150.010.6147245
CTAGGTG909.426366E-510.5655834
AATGTCC3250.010.5225368
GTATCAA48700.010.4711551
CCCTATA1002.3673547E-510.4627372
GTATTAT1659.058567E-1010.3788041
CTAATAC1201.5004807E-610.3042113
AGGACCT8700.010.2741185
ACGAAAT1409.79926E-810.17085312
Produced by FastQC (version 0.11.5)