FastQCFastQC Report
Thu 26 May 2016
SRR1512911_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512911_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803595
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63920.3544032889867182No Hit
GTCCTACAGTGGACATTTCTAAATT60020.3327798092143746No Hit
GTCCTAAAGTGTGTATTTCTCATTT53580.2970733451800432No Hit
CTGTAGGACGTGGAATATGGCAAGA52750.29247142512592905No Hit
CTTTAGGACGTGAAATATGGCGAGG45980.2549352820339378No Hit
TATCAACGCAGAGTACTTTTTTTTT36250.20098747224293703No Hit
GGTATCAACGCAGAGTACTTTTTTT33990.18845694293896356No Hit
GTCCTACAGTGTGCATTTCTCATTT32630.18091644742860785No Hit
GTACTGGTTCACTATCGGTCAGTCA23850.13223589553087028No Hit
CTGAAGGACCTGGAATATGGCGAGA23080.12796664439633068No Hit
GTCCTTCAGTGTGCATTTCTCATTT21120.11709945969022978No Hit
CTGTAGGACCTGGAATATGGCGAGA18720.10379270290724912No Hit
ATTTAGAAATGTCCACTGTAGGACG18460.1023511375890929No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA400.005282553414.24877312
GGTATCA17000.013.1077181
TAGGACC7500.012.7911654
TCCAACG2000.012.34859318
CCAACGA1251.4151738E-912.15793919
TGTCGAG951.0414988E-611.99863218
CAAGTCG2350.011.7217436
ATGTCGA1251.8344508E-811.39901717
ACTAGAC752.0774311E-411.3980683
GTCCTAC28650.011.3341741
GGCGAGG13900.011.138419
AGTCGTC2650.011.1116338
TGCACGA600.005883690411.0814552
TAGTACC1303.2745447E-810.95968154
GTATCAA37100.010.93442251
AGGACCT15900.010.932115
AAATGTC6200.010.8774917
TATGTCG1053.4713448E-610.85590616
TCCTACA31600.010.7908932
CCTACAG30000.010.7331813
Produced by FastQC (version 0.11.5)