##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512911_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1803595
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.969786454276043	31.0	30.0	34.0	22.0	34.0
2	29.049897011246983	31.0	30.0	34.0	22.0	34.0
3	29.215845020639335	31.0	30.0	34.0	23.0	34.0
4	32.62369767048589	35.0	33.0	37.0	25.0	37.0
5	32.81624755003202	35.0	33.0	37.0	26.0	37.0
6	32.693866971243544	36.0	33.0	37.0	25.0	37.0
7	32.66774358988575	37.0	33.0	37.0	25.0	37.0
8	32.576811312961055	37.0	33.0	37.0	24.0	37.0
9	33.862194117859055	39.0	34.0	39.0	22.0	39.0
10	33.733797776108275	38.0	34.0	39.0	22.0	39.0
11	33.787778852791234	39.0	34.0	39.0	22.0	39.0
12	33.69484723565989	39.0	34.0	39.0	20.0	39.0
13	33.65393949306801	38.0	34.0	39.0	19.0	39.0
14	34.52107097214175	39.0	34.0	41.0	17.0	41.0
15	34.70051702294584	39.0	34.0	41.0	19.0	41.0
16	34.64557675087811	39.0	34.0	41.0	19.0	41.0
17	34.42526010551149	39.0	34.0	41.0	17.0	41.0
18	34.41844427379761	39.0	34.0	41.0	17.0	41.0
19	34.53071837080941	39.0	34.0	41.0	18.0	41.0
20	34.51418749774756	39.0	34.0	41.0	18.0	41.0
21	34.414202745073034	39.0	34.0	41.0	18.0	41.0
22	34.429336963120875	39.0	34.0	41.0	18.0	41.0
23	34.10060351686493	39.0	34.0	41.0	10.0	41.0
24	34.16138878184958	39.0	34.0	41.0	2.0	41.0
25	33.88356975928632	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6636043082399787
1101	2	0.346388546487038
1101	3	0.7227359011131611
1101	4	0.8146194089837024
1101	5	0.682814877180661
1101	6	0.6599524335375335
1101	7	0.3862086384476058
1101	8	0.7306917961437307
1101	9	0.7394268785365625
1101	10	0.6475699919847457
1101	11	0.6834026063752319
1101	12	0.6465792464795115
1101	13	1.1420887921868044
1101	14	1.120112607650647
1101	15	0.9678084340100312
1101	16	0.9392598225685305
1101	17	0.30287594921762206
1101	18	0.6017474914233532
1101	19	0.1196474764874722
1101	20	-0.5750957053425552
1101	21	0.67270250668507
1101	22	0.775413348958935
1101	23	0.4235895792030746
1101	24	0.845296106576022
1101	25	0.3522965895500789
1102	1	1.0526272476650966
1102	2	1.1437067377909855
1102	3	1.0981620569184827
1102	4	1.1876701251023931
1102	5	1.0483463600181153
1102	6	1.0021640670250633
1102	7	0.8634106364294354
1102	8	0.9992839246407357
1102	9	1.30590965614347
1102	10	1.3731014540055497
1102	11	1.1508348312741319
1102	12	1.2930008127344763
1102	13	1.1892063630505731
1102	14	1.5510176009919192
1102	15	1.4139855607818532
1102	16	1.2970239637074812
1102	17	1.5230090040242104
1102	18	1.4094628175239379
1102	19	1.111000631432546
1102	20	1.4697847264363446
1102	21	1.5406813767470382
1102	22	1.3689370817585242
1102	23	1.2581254642410613
1102	24	0.721573012743427
1102	25	0.9842232639501276
1103	1	0.6282266822882825
1103	2	0.6781917090470984
1103	3	0.8689270186660067
1103	4	1.0752183659820176
1103	5	0.8274773774330981
1103	6	0.5171711889261417
1103	7	0.41586793077858175
1103	8	0.5387046868615784
1103	9	0.7612325887921259
1103	10	0.8650220168589513
1103	11	0.7057978595835408
1103	12	0.6239828185350191
1103	13	0.7998156108763226
1103	14	0.8057949473050598
1103	15	0.7887982603162342
1103	16	0.8540840704158015
1103	17	0.4838186431570577
1103	18	0.8881030285443643
1103	19	0.7623436684594367
1103	20	0.9160246437538646
1103	21	0.7270620175896738
1103	22	0.8402490925054167
1103	23	0.18278924551710674
1103	24	0.43616456004718174
1103	25	0.7838913537251315
1104	1	0.8208815665220612
1104	2	0.7679833093607513
1104	3	0.7769499130171091
1104	4	0.8697212631641804
1104	5	0.7357394220185967
1104	6	0.5525151350167619
1104	7	0.792134318809147
1104	8	0.585551684725246
1104	9	0.8576939475901142
1104	10	0.8932119438659782
1104	11	0.5787099979479748
1104	12	0.8765597368123181
1104	13	0.8446223815845215
1104	14	1.0058213953839399
1104	15	0.8116095420981608
1104	16	0.8643369848264655
1104	17	1.043797857202108
1104	18	0.9954792487413826
1104	19	0.6191761198797181
1104	20	0.7503598377654228
1104	21	0.9886566132413819
1104	22	0.7043234749334673
1104	23	1.0531798761920328
1104	24	0.9977820649503215
1104	25	0.6907043415944045
1105	1	0.7274996028771419
1105	2	0.9262433540692605
1105	3	0.9535150786150837
1105	4	1.122907970225377
1105	5	0.9355604899245549
1105	6	1.1682690028913427
1105	7	1.1440795312726237
1105	8	1.1232280431384822
1105	9	0.8622286506118613
1105	10	0.9930408553705803
1105	11	1.0625787794449195
1105	12	1.1495395199368161
1105	13	1.193007335601422
1105	14	1.2855715522559166
1105	15	1.287895156938248
1105	16	1.259897507994495
1105	17	1.5461989788367134
1105	18	1.4635192805079598
1105	19	1.0642808564328519
1105	20	1.1872656851682137
1105	21	1.1373586202221873
1105	22	1.1871995509771551
1105	23	1.3531476439038599
1105	24	1.4425190102608312
1105	25	1.6965832413335988
1106	1	0.742286487206318
1106	2	0.7025660153090101
1106	3	0.6040257743415864
1106	4	0.7882076722508842
1106	5	0.6069821997229639
1106	6	0.7125637499924764
1106	7	0.682803233825382
1106	8	0.6929253903266996
1106	9	0.8237250088123531
1106	10	0.5926591005165065
1106	11	0.4288810747672045
1106	12	0.6742472373163935
1106	13	0.7458251617456924
1106	14	1.0693474452806768
1106	15	0.8883539517792158
1106	16	0.8995920242253206
1106	17	0.941407620895852
1106	18	0.9109945358433365
1106	19	0.9050386293331485
1106	20	1.0376605712708624
1106	21	0.8890805363259915
1106	22	0.8553453138278044
1106	23	1.1232333365347813
1106	24	0.9060600736223492
1106	25	1.1044272151390047
1107	1	0.7922788346693039
1107	2	0.86940132234648
1107	3	0.8635429998670361
1107	4	0.9850721442959554
1107	5	0.8902037531696863
1107	6	0.8280604728163681
1107	7	0.6821555426049457
1107	8	0.9108445222180208
1107	9	0.8957578824040269
1107	10	1.1431839547981824
1107	11	1.0652216620552082
1107	12	1.0286065309414951
1107	13	0.9149075707277561
1107	14	1.11056516819054
1107	15	0.8840326769336215
1107	16	1.1201977758843356
1107	17	1.2948942204193798
1107	18	1.228575797554619
1107	19	1.194881609029835
1107	20	1.2921539519787828
1107	21	1.079865052469188
1107	22	1.1137361834669832
1107	23	1.427523330492022
1107	24	1.055525158236236
1107	25	1.365950929771941
1108	1	0.7650386059359029
1108	2	0.7578490888638321
1108	3	0.7771189420313682
1108	4	0.9199854306843491
1108	5	0.7328156736808396
1108	6	0.8214184518530487
1108	7	0.8593456743120171
1108	8	0.8478669286116656
1108	9	0.9786407726856723
1108	10	0.9237210840860541
1108	11	0.6942962397501873
1108	12	0.8061819521906131
1108	13	0.8827687444764436
1108	14	0.9311658397488074
1108	15	0.8730438296814498
1108	16	0.579557355210099
1108	17	0.967804752057944
1108	18	1.0193445698606993
1108	19	0.8995366434876786
1108	20	0.990116751123729
1108	21	0.9310060210429825
1108	22	0.9208955999480892
1108	23	1.3818355464231118
1108	24	1.0586366880609788
1108	25	1.2229155896635788
1109	1	0.7798644873288509
1109	2	0.5939524000373453
1109	3	0.7691590057108577
1109	4	0.9000095904910665
1109	5	0.6939579845423509
1109	6	0.757860960312982
1109	7	0.6531133241708247
1109	8	0.733233845676331
1109	9	0.8228000194117016
1109	10	0.8307838275841064
1109	11	0.692928250694095
1109	12	0.6803649587265639
1109	13	0.8878767036127684
1109	14	1.032115032155268
1109	15	0.8570499576324266
1109	16	0.538867680727499
1109	17	1.0553680515515467
1109	18	0.96340501737712
1109	19	0.8143713248102245
1109	20	0.7525466516845256
1109	21	0.7302916267581381
1109	22	0.7058673201743275
1109	23	0.9855147169897478
1109	24	0.6450299969145092
1109	25	0.5052653308656474
1110	1	0.5036824355607763
1110	2	0.5164357793539835
1110	3	0.44723163827265466
1110	4	0.5161062045753013
1110	5	0.33706434989550615
1110	6	0.4606178525435638
1110	7	0.4229567001176946
1110	8	0.4004642466086352
1110	9	0.6316512792138695
1110	10	0.5535869839343661
1110	11	0.40159171608025446
1110	12	0.5117041610741708
1110	13	0.5683713576147227
1110	14	0.5849003568114455
1110	15	0.5165993914410407
1110	16	0.5780279062212017
1110	17	0.7242124314087519
1110	18	-0.07077426837226852
1110	19	0.4009013754269617
1110	20	0.4602220025266206
1110	21	0.5969249467528002
1110	22	0.6972340423017727
1110	23	0.8952944183439726
1110	24	0.716653383111094
1110	25	0.9621382554740876
1111	1	0.7689544040455552
1111	2	0.7481362724810836
1111	3	0.8286556246484977
1111	4	0.709670755567636
1111	5	0.9183414274390458
1111	6	0.8282113548688343
1111	7	0.6880883098454902
1111	8	0.8988861463176221
1111	9	0.9666562853428644
1111	10	0.9461400161513751
1111	11	0.7281859576283125
1111	12	0.363528824914944
1111	13	0.7649311644810766
1111	14	0.843139883708858
1111	15	0.8950337362931506
1111	16	1.001323716980167
1111	17	0.7278327516138603
1111	18	0.8367274393259407
1111	19	0.9476754633289772
1111	20	1.0554161186875248
1111	21	1.0173878499333284
1111	22	0.780443392066438
1111	23	1.1450511298318702
1111	24	1.0243969757338363
1111	25	1.0113218353709712
1112	1	0.3232704555104604
1112	2	0.33458435934704767
1112	3	0.3186830173189712
1112	4	0.4323102024248655
1112	5	0.38164626066186713
1112	6	0.10690754187122309
1112	7	-0.006032712958521813
1112	8	0.14187915673964113
1112	9	0.1222678063584226
1112	10	0.2640006396463903
1112	11	0.17547036093538537
1112	12	0.3048084640338047
1112	13	0.2705622853029368
1112	14	0.35587975780892833
1112	15	0.0429238042030633
1112	16	0.350415561249001
1112	17	0.6487438861391226
1112	18	0.3927398269682243
1112	19	0.3649614572085653
1112	20	0.1965803707113878
1112	21	0.31223246558653983
1112	22	0.17315280529106047
1112	23	0.2188059303726675
1112	24	0.2934004349937709
1112	25	0.2486101962859948
1113	1	0.03909629533679393
1113	2	0.22264095774222525
1113	3	0.159892546972479
1113	4	0.33352405427250886
1113	5	-0.0224091128738948
1113	6	-0.25492203957728776
1113	7	-0.2501217320891058
1113	8	-0.2041496906966529
1113	9	-0.2934913699236219
1113	10	-0.2450389987728272
1113	11	-0.19635352177861876
1113	12	-0.29690229749257924
1113	13	-0.2364307300028088
1113	14	0.20225131387251594
1113	15	-0.32614949407564353
1113	16	-0.18623393472087457
1113	17	0.0034941510458779135
1113	18	-0.15439431819901017
1113	19	-0.5087018543291535
1113	20	-0.3383455968550422
1113	21	-0.180548780750712
1113	22	-0.6906404264572359
1113	23	-4.1826662308218765
1113	24	-1.7301527889755306
1113	25	-0.8740804432632245
1114	1	-0.9374800206111189
1114	2	-1.2471292061115697
1114	3	-1.0329550619580985
1114	4	-0.850549973314358
1114	5	-0.9737330968364866
1114	6	-1.6198730222204425
1114	7	-1.4267061606213183
1114	8	-1.2176438776983574
1114	9	-1.4419304882949007
1114	10	-1.1410997013124202
1114	11	-1.3929316439245412
1114	12	-2.0249271434300127
1114	13	-1.3602180517172897
1114	14	-3.3328736250279896
1114	15	-2.011745779226345
1114	16	-2.1426499684528935
1114	17	-1.8581799087872355
1114	18	-1.8371652300432828
1114	19	-1.9938752136227507
1114	20	-1.447610117105377
1114	21	-1.314596225592794
1114	22	-1.5634794942141568
1114	23	-5.790746517854291
1114	24	-2.9241509562457964
1114	25	-2.9484988628093483
1115	1	-1.750088722652329
1115	2	-1.574371551441093
1115	3	-1.306167047863699
1115	4	-1.4673666842228208
1115	5	-1.2850515443535429
1115	6	-1.523448997927865
1115	7	-1.9787991702075658
1115	8	-2.136366239695967
1115	9	-3.3624781524015255
1115	10	-4.210560633654488
1115	11	-3.0349866465013804
1115	12	-3.1508777122756015
1115	13	-2.470488761128987
1115	14	-3.2735208672893457
1115	15	-3.0617471437420107
1115	16	-2.9913734640481344
1115	17	-2.904060435994108
1115	18	-5.49076036145139
1115	19	-3.5168221686010845
1115	20	-3.7646587431955467
1115	21	-3.4914610724154116
1115	22	-2.988030699763744
1115	23	-3.6465710054524187
1115	24	-3.3738084628138374
1115	25	-3.5100107041812514
1116	1	-1.8803776673536277
1116	2	-1.6412520845239627
1116	3	-1.9756507541209203
1116	4	-1.7722652003246893
1116	5	-1.9344498896031084
1116	6	-4.195503187421721
1116	7	-2.773845401004447
1116	8	-3.0933721611939227
1116	9	-4.119897791118493
1116	10	-3.4106029173782737
1116	11	-3.007154599347327
1116	12	-2.798830475315601
1116	13	-4.373045209011927
1116	14	-7.509273650819779
1116	15	-5.196892202379587
1116	16	-4.077255615638244
1116	17	-5.1094962633367516
1116	18	-3.8590681774852094
1116	19	-3.5438218659822773
1116	20	-4.401137752643056
1116	21	-5.179309772487009
1116	22	-4.699078003643365
1116	23	-3.7474683205544004
1116	24	-4.7353316362487625
1116	25	-4.493438623164689
1201	1	0.5291195952402674
1201	2	0.37352403765079245
1201	3	0.7423044613281249
1201	4	0.7229495058871436
1201	5	0.3680381276929623
1201	6	0.6470035564997616
1201	7	0.8433796198278785
1201	8	0.8109339442490793
1201	9	1.3744641527363797
1201	10	1.265972805942127
1201	11	1.3796068266798045
1201	12	1.1274677333143401
1201	13	1.335857006972077
1201	14	1.3817542827636018
1201	15	1.3153244346036388
1201	16	1.3172756226613558
1201	17	1.4507563244540407
1201	18	1.2946971262593365
1201	19	1.3684460505638327
1201	20	1.3906660239529742
1201	21	1.542117297011778
1201	22	1.356526175641008
1201	23	1.7322984173405374
1201	24	1.57992385398925
1201	25	1.6837409529522134
1202	1	0.7926267486739071
1202	2	0.764762570219105
1202	3	0.886991771869134
1202	4	1.0181278633982345
1202	5	0.8868051814034601
1202	6	1.041023702449941
1202	7	1.0755682654664653
1202	8	1.0307121374759305
1202	9	1.3165804687430267
1202	10	1.0875011872352403
1202	11	1.056913164532105
1202	12	1.3251386315679952
1202	13	1.348788051391061
1202	14	1.6896911785599116
1202	15	1.371618295921003
1202	16	1.088615860850453
1202	17	1.3400661692907292
1202	18	1.3568819375745065
1202	19	1.3352822265984656
1202	20	1.214278694278768
1202	21	1.3507065656702935
1202	22	1.4607924943754824
1202	23	1.5121606122179116
1202	24	1.4729681300513136
1202	25	1.8391532272438837
1203	1	1.1008607789150133
1203	2	1.1809984153904445
1203	3	1.07430962891981
1203	4	1.3513830185743956
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2211	20	0.1800379486025534
2211	21	0.2916520602503283
2211	22	0.313147695839163
2211	23	0.4717472646953169
2211	24	0.18609677073698094
2211	25	0.4949986525347754
2212	1	0.1121658289181191
2212	2	0.20154982497222917
2212	3	-0.17230617685339666
2212	4	0.08830508412712135
2212	5	-0.11061472173314257
2212	6	-0.07460623709229708
2212	7	0.07640808066991411
2212	8	0.07897565117045957
2212	9	0.162073448081415
2212	10	-0.019442227750310792
2212	11	-0.3690465392218698
2212	12	0.11675618188249359
2212	13	0.20713686381720464
2212	14	0.24938615112917262
2212	15	-0.08948304153214792
2212	16	-0.14283043711613175
2212	17	-0.04578556779092935
2212	18	0.32275314259211285
2212	19	0.05104143144937012
2212	20	0.09709675242658022
2212	21	0.288881729465885
2212	22	0.3034765393005685
2212	23	0.3337197884920329
2212	24	0.349106364303573
2212	25	0.5503535415885352
2213	1	-0.25648226189715473
2213	2	-0.1456623230448706
2213	3	-0.44940996573737024
2213	4	-0.31233991071292166
2213	5	-0.276533931522323
2213	6	-0.028454829160700967
2213	7	-0.3098037724924083
2213	8	-0.0656653631299946
2213	9	-0.5930032141624295
2213	10	-0.26113911703234294
2213	11	-0.2945020898631796
2213	12	-0.3457788321231092
2213	13	-0.4963475825929393
2213	14	-0.5051605096403975
2213	15	-0.2717357320820568
2213	16	-0.3126631070262036
2213	17	-0.3762315356517547
2213	18	-0.33639546421903077
2213	19	-0.45438684512441796
2213	20	-0.26444681236775835
2213	21	-0.3879264674971097
2213	22	-0.6300735122990133
2213	23	-0.41066691355720764
2213	24	-0.14562015788425242
2213	25	0.07336990602905047
2214	1	-2.960783017407806
2214	2	-1.586974204704834
2214	3	-1.7398451627483702
2214	4	-3.6415411281716352
2214	5	-2.12459673612528
2214	6	-1.7287476292522008
2214	7	-1.6251408293685685
2214	8	-1.4006545928141314
2214	9	-1.3433597465655112
2214	10	-1.324068134614322
2214	11	-1.2224263735895207
2214	12	-1.2799814157280878
2214	13	-1.7040314245774724
2214	14	-1.5619447919688199
2214	15	-1.898001450706758
2214	16	-1.747635180454978
2214	17	-3.955132772808394
2214	18	-3.672507348420659
2214	19	-2.487574159662117
2214	20	-2.1683819490141545
2214	21	-2.2218522635155864
2214	22	-3.1585345961931033
2214	23	-2.1847336450363954
2214	24	-3.189116856893637
2214	25	-3.041782117455959
2215	1	-1.7294212332571774
2215	2	-3.576027445252098
2215	3	-2.0875363714953536
2215	4	-3.523413534944442
2215	5	-2.1647308126139393
2215	6	-2.004359762434053
2215	7	-2.13464257891016
2215	8	-1.4102747498446462
2215	9	-4.071811131391399
2215	10	-2.264345744712653
2215	11	-1.7274463093863446
2215	12	-1.6032574646017466
2215	13	-1.8414547950685716
2215	14	-1.7465767639513885
2215	15	-1.7452385061111428
2215	16	-1.7931811757021663
2215	17	-2.0070277569742956
2215	18	-1.688140103595245
2215	19	-1.812834888309304
2215	20	-1.6056074061599332
2215	21	-2.247879983766417
2215	22	-2.2346079252544797
2215	23	-2.280958561480432
2215	24	-2.1839294996146457
2215	25	-2.995311305546899
2216	1	-3.607064586252644
2216	2	-3.602335578954598
2216	3	-4.8868170985929815
2216	4	-5.524336451360316
2216	5	-5.210570666552385
2216	6	-4.189013267510095
2216	7	-5.9291432008755685
2216	8	-3.8061356703534557
2216	9	-4.404993989222145
2216	10	-3.5197578469557413
2216	11	-3.7490997342087837
2216	12	-4.215497141982265
2216	13	-3.7836822337748863
2216	14	-4.932890515405987
2216	15	-4.158343919216556
2216	16	-4.7031416508034845
2216	17	-4.3143890185609095
2216	18	-3.8625662903453772
2216	19	-4.545409825109591
2216	20	-5.101243616584878
2216	21	-4.852420331264511
2216	22	-4.454693395339952
2216	23	-4.353332262375261
2216	24	-4.785686050314858
2216	25	-4.855815498797572
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88153.0
3	0.0
4	768.0
5	4958.0
6	6059.0
7	5564.0
8	4884.0
9	4488.0
10	4004.0
11	3677.0
12	3514.0
13	3475.0
14	3326.0
15	3386.0
16	3506.0
17	3702.0
18	4302.0
19	5186.0
20	6875.0
21	8753.0
22	11070.0
23	13189.0
24	15676.0
25	17486.0
26	19292.0
27	21428.0
28	23965.0
29	28173.0
30	34350.0
31	42225.0
32	52914.0
33	68069.0
34	87896.0
35	115353.0
36	157513.0
37	241520.0
38	684896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14673631857403	18.649131298295977	12.803544903783568	24.40058747934643
2	14.439438509716288	20.04236407038868	42.92733508371617	22.59086233617886
3	19.342489543588666	25.81074269770145	28.96754478526859	25.879222973441294
4	12.220001730061595	16.57626355492169	38.12462432156078	33.07911039345593
5	12.783362010789673	39.275509304415955	34.700350617253825	13.24077806754054
6	31.343655429032445	36.33295195142874	18.766708577985572	13.556684041553241
7	28.43574783532669	31.85383704122936	21.644125211196098	18.06628991224785
8	25.324859486096795	37.2782122557761	20.428254519560944	16.96867373856616
9	28.014706820770886	14.72446629233746	20.004250462085157	37.2565764248065
10	16.564772604305688	28.592785378752357	33.2924166289778	21.550025387964165
11	34.11120877559809	22.158629323318284	25.25048721261115	18.479674688472482
12	24.939911016692832	24.493329581760698	31.155756305389108	19.41100309615736
13	29.60691212301635	22.395871192847277	25.65594642449895	22.341270259637426
14	21.08319307725987	23.26669296988282	27.348408294724763	28.301705658132548
15	22.984062051568323	32.39689608981452	23.071292937072794	21.54774892154436
16	21.47855651350723	27.979883569750776	28.80153198280459	21.7400279339374
17	21.172976845811338	29.173911426806605	27.938310994590644	21.714800732791417
18	21.039536784877242	26.791875642745396	31.311475318854438	20.857112253522924
19	23.761953703539273	26.366518087052267	27.779430306689633	22.09209790271883
20	24.58111657481466	26.666751757637236	28.347834869502826	20.404296798045277
21	23.401521045044284	26.36931231140917	26.994720843954717	23.23444579959183
22	23.981219146037233	28.586857287154483	26.85523369231922	20.576689874489063
23	22.003169892239203	28.23801273506427	28.311155556690125	21.447661816006402
24	23.490018173998696	26.482318502795465	28.907090634147696	21.120572689058143
25	22.588462353765067	27.46170229059313	28.684299021234516	21.265536334407287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	282.0
2	610.0
3	938.0
4	938.0
5	938.0
6	1604.5
7	2271.0
8	2271.0
9	2271.0
10	2637.5
11	3004.0
12	3004.0
13	3004.0
14	4349.5
15	5695.0
16	5695.0
17	5695.0
18	11363.5
19	17032.0
20	17032.0
21	17032.0
22	29149.5
23	41267.0
24	41267.0
25	41267.0
26	64812.0
27	88357.0
28	88357.0
29	88357.0
30	116243.5
31	144130.0
32	144130.0
33	144130.0
34	161436.5
35	178743.0
36	178743.0
37	178743.0
38	189176.5
39	199610.0
40	199610.0
41	199610.0
42	210533.0
43	221456.0
44	221456.0
45	221456.0
46	234012.0
47	246568.0
48	246568.0
49	246568.0
50	234758.5
51	222949.0
52	222949.0
53	222949.0
54	194129.0
55	165309.0
56	165309.0
57	165309.0
58	141491.0
59	117673.0
60	117673.0
61	117673.0
62	97024.0
63	76375.0
64	76375.0
65	76375.0
66	58330.0
67	40285.0
68	40285.0
69	40285.0
70	29828.0
71	19371.0
72	19371.0
73	19371.0
74	13778.0
75	8185.0
76	8185.0
77	8185.0
78	5503.0
79	2821.0
80	2821.0
81	2821.0
82	1856.0
83	891.0
84	891.0
85	891.0
86	598.5
87	306.0
88	306.0
89	306.0
90	182.0
91	58.0
92	58.0
93	58.0
94	35.5
95	13.0
96	13.0
97	13.0
98	9.5
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33738172926848875
2	0.009980067587235493
3	0.008649391908937428
4	0.01058996060645544
5	0.011310743265533559
6	0.07634751704235153
7	0.07540495510355706
8	0.10068779299122033
9	0.07972965105802578
10	0.08649391908937427
11	0.08355534363313272
12	0.09336907676058094
13	0.07917520285873492
14	0.08505235377121804
15	0.08211377831497647
16	0.08239100241462191
17	0.09525420063816988
18	0.09725021415561698
19	0.09109583914348841
20	0.08882260152639589
21	0.07834353055979862
22	0.0975828830751915
23	0.09098494950363026
24	0.08704836728866513
25	0.08477512967157261
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1803595.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.62417893689439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83483905283646	41.42415818072725
2	13.698217836371738	14.96507804421056
3	4.640839257412002	7.605061020427117
4	2.079451125179284	4.543532414092782
5	1.1245538086806965	3.0713914234770234
6	0.6578355728701215	2.156023682610717
7	0.4312620814685343	1.6490135974784321
8	0.2866849127756602	1.2527942379172496
9	0.19758283071263064	0.9713519909734363
>10	0.9353873432678836	8.830561470186897
>50	0.060760279827520414	2.3175115429166984
>100	0.042951013767407335	4.723488239349286
>500	0.006457422811659591	2.490115851752611
>1k	0.0027674669192826813	2.710656972383382
>5k	4.099950991529898E-4	1.289261331496512
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6392	0.3544032889867182	No Hit
GTCCTACAGTGGACATTTCTAAATT	6002	0.3327798092143746	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5358	0.2970733451800432	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5275	0.29247142512592905	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4598	0.2549352820339378	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3625	0.20098747224293703	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3399	0.18845694293896356	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3263	0.18091644742860785	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2385	0.13223589553087028	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2308	0.12796664439633068	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2112	0.11709945969022978	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1872	0.10379270290724912	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1846	0.1023511375890929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.544481992908607E-5	5.544481992908607E-5
5	0.0	0.0	0.0	5.544481992908607E-5	5.544481992908607E-5
6	5.544481992908607E-5	0.0	0.0	5.544481992908607E-5	5.544481992908607E-5
7	5.544481992908607E-5	0.0	0.0	1.6633445978725823E-4	5.544481992908607E-5
8	5.544481992908607E-5	0.0	0.0	1.6633445978725823E-4	5.544481992908607E-5
9	5.544481992908607E-5	0.0	0.0	2.772240996454304E-4	1.1088963985817215E-4
10	5.544481992908607E-5	0.0	0.0	2.772240996454304E-4	1.1088963985817215E-4
11	5.544481992908607E-5	0.0	0.0	2.772240996454304E-4	1.1088963985817215E-4
12	5.544481992908607E-5	0.0	0.0	2.772240996454304E-4	1.1088963985817215E-4
13	5.544481992908607E-5	0.0	0.0	2.772240996454304E-4	1.1088963985817215E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCA	40	0.0052825534	14.248773	12
GGTATCA	1700	0.0	13.107718	1
TAGGACC	750	0.0	12.791165	4
TCCAACG	200	0.0	12.348593	18
CCAACGA	125	1.4151738E-9	12.157939	19
TGTCGAG	95	1.0414988E-6	11.998632	18
CAAGTCG	235	0.0	11.721743	6
ATGTCGA	125	1.8344508E-8	11.399017	17
ACTAGAC	75	2.0774311E-4	11.398068	3
GTCCTAC	2865	0.0	11.334174	1
GGCGAGG	1390	0.0	11.1384	19
AGTCGTC	265	0.0	11.111633	8
TGCACGA	60	0.0058836904	11.081455	2
TAGTACC	130	3.2745447E-8	10.9596815	4
GTATCAA	3710	0.0	10.9344225	1
AGGACCT	1590	0.0	10.93211	5
AAATGTC	620	0.0	10.877491	7
TATGTCG	105	3.4713448E-6	10.855906	16
TCCTACA	3160	0.0	10.790893	2
CCTACAG	3000	0.0	10.733181	3
>>END_MODULE