FastQCFastQC Report
Thu 26 May 2016
SRR1512911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803595
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81470.45170894796226424No Hit
GTCCTACAGTGGACATTTCTAAATT57390.318197821573025No Hit
TATCAACGCAGAGTACTTTTTTTTT57370.31808693193316684No Hit
CTGTAGGACGTGGAATATGGCAAGA50970.28260224717855176No Hit
GTCCTAAAGTGTGTATTTCTCATTT49120.27234495549167076No Hit
CTTTAGGACGTGAAATATGGCGAGG48490.26885193183613837No Hit
GGTATCAACGCAGAGTACTTTTTTT46950.2603134295670591No Hit
GTCCTACAGTGTGCATTTCTCATTT30190.16738791136591086No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26850.14886934150959613No Hit
CTGAAGGACCTGGAATATGGCGAGA24300.13473091242767915No Hit
GTACTGGTTCACTATCGGTCAGTCA21920.12153504528455668No Hit
TTTCTAAATTTTCCACCTTTTTCAG19640.10889362634072504No Hit
GTCCTTCAGTGTGCATTTCTCATTT19290.10695305764320705No Hit
CTGTAGGACCTGGAATATGGCGAGA18600.1031273650681001No Hit
ATTTAGAAATGTCCACTGTAGGACG18550.10285014096845467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.013.4721074
GGTATCA17600.012.9169991
GGACGTA550.003045352912.1032836
TGGACCG657.9559424E-411.7042735
AAATGTC6450.011.2002767
GGACGGG855.268537E-511.1879086
AAACGGG855.3707925E-511.16681619
TACACCG600.005831700711.0946765
ACGTGTA600.00587282411.08420858
GGCGAGG14850.010.99386219
AGGACCT17200.010.9472385
TAGAAAT6950.010.9464444
GTTCTAA1605.329639E-1010.7011131
TGTACCG1252.2088716E-710.6508885
GTATCAA38550.010.6347991
CGAAATC2500.010.63228213
CTAGGAC2600.010.60788253
TGTAGGA29050.010.5745132
AATGTCC6750.010.5563898
TAGGACA6950.010.5359524
Produced by FastQC (version 0.11.5)