##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512911_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1803595 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.214130666807126 31.0 30.0 34.0 26.0 34.0 2 30.238949986000183 31.0 30.0 34.0 26.0 34.0 3 30.41174321286098 31.0 30.0 34.0 26.0 34.0 4 33.987409035842305 37.0 35.0 37.0 30.0 37.0 5 33.57433126616563 37.0 35.0 37.0 28.0 37.0 6 33.43451218261306 37.0 35.0 37.0 28.0 37.0 7 33.261784935087974 37.0 35.0 37.0 27.0 37.0 8 33.219443389452735 37.0 35.0 37.0 27.0 37.0 9 34.53285410527308 39.0 35.0 39.0 27.0 39.0 10 34.44989091231679 39.0 35.0 39.0 27.0 39.0 11 34.42546580579343 39.0 35.0 39.0 26.0 39.0 12 34.296171812408 39.0 35.0 39.0 25.0 39.0 13 34.27932434942434 39.0 35.0 39.0 25.0 39.0 14 35.34672140918554 40.0 36.0 41.0 25.0 41.0 15 35.35502648876272 40.0 36.0 41.0 25.0 41.0 16 35.35555765013764 40.0 36.0 41.0 25.0 41.0 17 35.35317740401808 40.0 36.0 41.0 25.0 41.0 18 35.278464954715446 40.0 36.0 41.0 25.0 41.0 19 35.292576215835595 40.0 35.0 41.0 25.0 41.0 20 35.2692705402266 40.0 36.0 41.0 25.0 41.0 21 35.235237400857734 40.0 36.0 41.0 25.0 41.0 22 35.20119705366227 40.0 36.0 41.0 25.0 41.0 23 35.03906697457023 40.0 35.0 41.0 24.0 41.0 24 34.99645707600653 40.0 35.0 41.0 24.0 41.0 25 34.932841352964495 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6695522005506547 1101 2 0.45694529402953066 1101 3 0.44331610396629983 1101 4 0.4289536939850649 1101 5 0.61906480371443 1101 6 0.670395012773298 1101 7 0.7043923224947122 1101 8 0.508466886315901 1101 9 0.8262955160705019 1101 10 0.48415397116540504 1101 11 0.7476312683969937 1101 12 0.8353739941033922 1101 13 0.904492922831956 1101 14 1.1574403014641987 1101 15 0.7999712537811874 1101 16 0.8985003514878898 1101 17 1.0282876206610752 1101 18 0.9851632363429985 1101 19 0.6180529727296715 1101 20 0.8099944070410316 1101 21 0.9470301522033395 1101 22 0.8334840090185764 1101 23 0.9842611799436156 1101 24 1.0545621564778855 1101 25 1.023858053936948 1102 1 0.7342416239763558 1102 2 0.8464999686939549 1102 3 0.5579757595860926 1102 4 0.6461608388829774 1102 5 0.8437678932692094 1102 6 0.8411090862321231 1102 7 0.8267607729919462 1102 8 0.8819142896733183 1102 9 0.9206651853824468 1102 10 0.8074190727324293 1102 11 1.0178990069128417 1102 12 0.9670162610571182 1102 13 0.9818948126712996 1102 14 1.0758152342654625 1102 15 1.1743741412583617 1102 16 1.141866476245184 1102 17 1.1589852021309284 1102 18 0.5513109960293789 1102 19 0.9312935574901218 1102 20 1.0644774021819075 1102 21 1.0551851258989018 1102 22 0.7727683534945342 1102 23 1.0462210204672786 1102 24 0.9625313676154192 1102 25 0.9538000171237471 1103 1 0.84668422554914 1103 2 0.9233405689864611 1103 3 0.7029759866169591 1103 4 0.5523848805742446 1103 5 0.6770741991183229 1103 6 0.7025841356669815 1103 7 0.8790318002792787 1103 8 1.0007061242442532 1103 9 1.052324264639367 1103 10 0.9833943229337976 1103 11 1.121491601865479 1103 12 1.2465561248157044 1103 13 1.0910273423535202 1103 14 1.0087515879106164 1103 15 1.1370573891911988 1103 16 1.120295109759745 1103 17 1.0544628617212268 1103 18 1.1340496293188167 1103 19 1.1416696439790854 1103 20 1.09500230645056 1103 21 1.1881027320091206 1103 22 1.1999832113102542 1103 23 1.2683855416604501 1103 24 1.239362123544261 1103 25 1.2537421337916612 1104 1 0.7615459970861629 1104 2 0.9474206135384371 1104 3 0.740486440127011 1104 4 0.6776470797506278 1104 5 0.791382796047742 1104 6 0.6531567738256001 1104 7 0.6005204918630156 1104 8 0.7170881015547792 1104 9 0.7818561522685599 1104 10 0.8220118706753752 1104 11 0.9141721891618317 1104 12 0.9434183023866112 1104 13 0.8382015886298291 1104 14 1.1449979350025359 1104 15 1.225158920013456 1104 16 1.0998987983354809 1104 17 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1107 17 1.1650167809298253 1107 18 1.1639853205782842 1107 19 1.0667340656062052 1107 20 1.1634984680016345 1107 21 1.1294929943514376 1107 22 1.1379806521409037 1107 23 1.27497689772121 1107 24 1.3360723644320274 1107 25 1.3839936023850896 1108 1 0.6011233768022031 1108 2 0.4952410225982007 1108 3 0.4654324248523274 1108 4 0.4163207033459315 1108 5 0.6599163022299521 1108 6 0.5662167719634184 1108 7 0.6408266301468899 1108 8 0.625497653126537 1108 9 0.8035041612834419 1108 10 0.7587732938614735 1108 11 0.6462761229062792 1108 12 0.8233259332826321 1108 13 0.8685956554249401 1108 14 0.8890720117292972 1108 15 0.9036596469814668 1108 16 0.7833821807302002 1108 17 0.9005045992571254 1108 18 1.056757152674642 1108 19 0.9727150873978232 1108 20 0.9574151403389237 1108 21 1.0142965835041977 1108 22 1.0218015417796238 1108 23 1.020401877717596 1108 24 1.0441404494585314 1108 25 1.107147579760479 1109 1 0.26140209457246044 1109 2 0.10936010964550391 1109 3 0.20182853368345732 1109 4 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22 0.46804223926351085 2209 23 0.60588166463139 2209 24 0.6328915973880669 2209 25 0.534736565733823 2210 1 -0.12943854089540707 2210 2 -1.132801584246419 2210 3 -0.2588592183367773 2210 4 0.06681764034586735 2210 5 0.0862814544984829 2210 6 0.08428856971726617 2210 7 -0.01799317369837894 2210 8 -0.016598496683116082 2210 9 -0.011005361800222602 2210 10 0.041004922800894406 2210 11 -0.09209475261158673 2210 12 0.1230310778273136 2210 13 0.20162879251263632 2210 14 0.16491173737176013 2210 15 0.1890013934393764 2210 16 0.08750942660152106 2210 17 -0.031060762032730338 2210 18 -0.10136307257359789 2210 19 -0.4173204345394339 2210 20 0.09167737346783156 2210 21 0.1577528085849096 2210 22 0.1443497903007156 2210 23 0.24293702588069976 2210 24 0.2943234405186317 2210 25 0.3193507336838408 2211 1 -0.3584295222230729 2211 2 -0.42444242964223733 2211 3 -0.12898973570426264 2211 4 -0.1590699049256159 2211 5 -0.10428735058471972 2211 6 -0.09281652699776544 2211 7 -0.30004296917137907 2211 8 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2214 4 -0.7706717818804307 2214 5 -1.034052382166898 2214 6 -1.5896310468099912 2214 7 -1.0161434252118227 2214 8 -0.9167936963369456 2214 9 -0.7478651655381157 2214 10 -1.0580021364406988 2214 11 -1.1994434735245605 2214 12 -0.9655215371762793 2214 13 -1.1360690584344582 2214 14 -0.7336075225895939 2214 15 -0.9093867620844236 2214 16 -0.9635059042882617 2214 17 -1.0929100025024852 2214 18 -0.9408714103026483 2214 19 -0.9891084306181597 2214 20 -1.2499790114459302 2214 21 -1.4816992862687925 2214 22 -1.739117473367351 2214 23 -1.684212075374397 2214 24 -1.4815149193658854 2214 25 -2.0166802032500044 2215 1 -1.6311979953960503 2215 2 -0.951144816571496 2215 3 -1.1395071458875172 2215 4 -1.1639390391915114 2215 5 -1.5627068655026548 2215 6 -1.4604102970713129 2215 7 -1.9181956669055005 2215 8 -1.7996546379628278 2215 9 -1.7725829223275866 2215 10 -1.86089262060608 2215 11 -1.4976131362360547 2215 12 -1.6745524170238966 2215 13 -1.7764871839123444 2215 14 -2.0699589570485983 2215 15 -1.8425131015852685 2215 16 -1.9661160284123298 2215 17 -2.2107984590363614 2215 18 -1.8189102778833544 2215 19 -2.059471629467417 2215 20 -2.1642603734364556 2215 21 -1.7847887930435533 2215 22 -2.021851339766556 2215 23 -2.44038487188584 2215 24 -2.2764571396069115 2215 25 -2.4793626255937795 2216 1 -2.192411429848921 2216 2 -1.464441835150204 2216 3 -1.423344070569513 2216 4 -1.8080193329196987 2216 5 -2.58151128747609 2216 6 -2.9024671968746922 2216 7 -2.804556567350911 2216 8 -3.0048129381915487 2216 9 -5.036426668565696 2216 10 -3.025763612941862 2216 11 -2.9668960703077225 2216 12 -3.4796515302215987 2216 13 -3.4278956346165685 2216 14 -3.6229514132100675 2216 15 -3.689971068761544 2216 16 -2.831430421895739 2216 17 -3.1378579670817857 2216 18 -3.5089357584017904 2216 19 -3.7536351863070294 2216 20 -2.946396924939201 2216 21 -3.72924828503826 2216 22 -3.0310303454244334 2216 23 -4.489174045675014 2216 24 -3.694809131902563 2216 25 -3.718653785659587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52079.0 3 0.0 4 879.0 5 7493.0 6 9096.0 7 7120.0 8 5716.0 9 4858.0 10 4132.0 11 3762.0 12 3351.0 13 3146.0 14 3098.0 15 3095.0 16 3073.0 17 3176.0 18 3594.0 19 4340.0 20 5741.0 21 7526.0 22 9659.0 23 11400.0 24 13712.0 25 15365.0 26 17054.0 27 18851.0 28 21324.0 29 25073.0 30 31426.0 31 39711.0 32 50535.0 33 65127.0 34 84805.0 35 111792.0 36 153160.0 37 236657.0 38 762669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.003351823858324 19.674151666699963 13.917838140203905 26.404658369237804 2 13.013406464546284 20.447784458175036 41.773764732050985 24.765044345227693 3 17.372497390775823 25.82205812053784 29.700235911575863 27.105208577110478 4 11.41155178207248 17.312036516433395 37.340293280368535 33.93611842112559 5 12.377809338480402 39.459535599234044 34.84125958628614 13.321395475999417 6 31.39959101202886 36.940789948171926 18.246289316271085 13.413329723528125 7 28.046722619130193 32.056433377262955 21.653270444613998 18.243573558992857 8 25.370361329750292 37.54795946218831 20.125221628746605 16.956457579314797 9 27.329128476618457 14.60608168025675 20.07954954460326 37.98524029852153 10 16.02731693895404 29.225862890534206 33.17024381882419 21.57657635168756 11 33.880228650261465 22.1820585288333 25.217291495813864 18.720421325091365 12 24.48534560811804 24.79927522290278 31.120005721924453 19.595373447054733 13 29.250890081270338 22.37426718351726 25.879698606089203 22.495144129123194 14 20.828232356163532 23.146626479837835 27.446417561555485 28.578723602443144 15 22.87072403589791 32.34649560592147 23.084272703478444 21.698507654702173 16 21.441566528614686 28.02492144123383 28.5950581864417 21.93845384370978 17 20.92808685358152 29.237120147269607 28.02691440785808 21.807878591290798 18 20.884486552994282 26.77507517381606 31.50225091725086 20.8381873559388 19 23.474803928907075 26.383670029469574 28.109816445852776 22.031709595770575 20 24.27437956042494 26.647186675242295 28.646842910687752 20.431590853645012 21 23.004243206185002 26.225653405515338 27.442172246559497 23.327931141740162 22 23.704703359923 28.315469817579785 27.27138146678029 20.708445355716925 23 21.643273573058252 27.97168987494421 28.77053884048248 21.614497711515057 24 23.360066977342477 26.36412276592029 29.1971867298368 21.078623526900444 25 22.237931596578832 27.37506334574189 28.957403939023973 21.429601118655306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 130.0 1 130.0 2 339.5 3 549.0 4 549.0 5 549.0 6 1354.0 7 2159.0 8 2159.0 9 2159.0 10 2549.5 11 2940.0 12 2940.0 13 2940.0 14 4351.0 15 5762.0 16 5762.0 17 5762.0 18 11693.0 19 17624.0 20 17624.0 21 17624.0 22 30696.5 23 43769.0 24 43769.0 25 43769.0 26 68247.0 27 92725.0 28 92725.0 29 92725.0 30 119396.5 31 146068.0 32 146068.0 33 146068.0 34 163422.0 35 180776.0 36 180776.0 37 180776.0 38 190320.0 39 199864.0 40 199864.0 41 199864.0 42 210615.0 43 221366.0 44 221366.0 45 221366.0 46 233217.5 47 245069.0 48 245069.0 49 245069.0 50 233400.5 51 221732.0 52 221732.0 53 221732.0 54 192530.5 55 163329.0 56 163329.0 57 163329.0 58 139532.0 59 115735.0 60 115735.0 61 115735.0 62 95234.5 63 74734.0 64 74734.0 65 74734.0 66 56889.0 67 39044.0 68 39044.0 69 39044.0 70 28875.5 71 18707.0 72 18707.0 73 18707.0 74 13243.5 75 7780.0 76 7780.0 77 7780.0 78 5237.0 79 2694.0 80 2694.0 81 2694.0 82 1727.0 83 760.0 84 760.0 85 760.0 86 499.5 87 239.0 88 239.0 89 239.0 90 138.0 91 37.0 92 37.0 93 37.0 94 20.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08821270850717594 2 0.04479941450270155 3 0.02090269711326545 4 0.0073741610505684485 5 0.020403693733903674 6 0.06082296746220742 7 0.08150388529575653 8 0.057163609346887745 9 0.024506610408656045 10 0.05012211721589381 11 0.007873164429930222 12 3.3266891957451646E-4 13 0.005988040552341296 14 0.0010534515786526355 15 0.014360208361633294 16 0.00931472974808646 17 0.005544481992908607 18 0.005988040552341296 19 0.002217792797163443 20 0.0019405686975180127 21 3.3266891957451646E-4 22 6.653378391490329E-4 23 0.0 24 0.0 25 5.544481992908607E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1803595.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.25254336487241 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60286510018409 39.727923088912796 2 14.294347464265861 15.224207164267433 3 4.929307183110481 7.87494433582104 4 2.2492155548208728 4.791057954801763 5 1.1755574266963913 3.13007114215237 6 0.7039407857762102 2.249198233251 7 0.4474623919246343 1.6679957301081283 8 0.2891055085948551 1.2316482906776813 9 0.21832382237484096 1.0463668936740829 >10 0.9727462472150147 9.015671217636697 >50 0.06011600078683183 2.2119170570099738 >100 0.0478076113030666 5.14037577718679 >500 0.0055438620020826215 2.140212341292101 >1k 0.00324263626536908 3.171437969170417 >5k 4.184046794024619E-4 1.37697280403781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8147 0.45170894796226424 No Hit GTCCTACAGTGGACATTTCTAAATT 5739 0.318197821573025 No Hit TATCAACGCAGAGTACTTTTTTTTT 5737 0.31808693193316684 No Hit CTGTAGGACGTGGAATATGGCAAGA 5097 0.28260224717855176 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4912 0.27234495549167076 No Hit CTTTAGGACGTGAAATATGGCGAGG 4849 0.26885193183613837 No Hit GGTATCAACGCAGAGTACTTTTTTT 4695 0.2603134295670591 No Hit GTCCTACAGTGTGCATTTCTCATTT 3019 0.16738791136591086 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2685 0.14886934150959613 No Hit CTGAAGGACCTGGAATATGGCGAGA 2430 0.13473091242767915 No Hit GTACTGGTTCACTATCGGTCAGTCA 2192 0.12153504528455668 No Hit TTTCTAAATTTTCCACCTTTTTCAG 1964 0.10889362634072504 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1929 0.10695305764320705 No Hit CTGTAGGACCTGGAATATGGCGAGA 1860 0.1031273650681001 No Hit ATTTAGAAATGTCCACTGTAGGACG 1855 0.10285014096845467 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1088963985817215E-4 0.0 3 0.0 0.0 0.0 1.1088963985817215E-4 0.0 4 0.0 0.0 0.0 1.1088963985817215E-4 5.544481992908607E-5 5 0.0 0.0 0.0 1.1088963985817215E-4 5.544481992908607E-5 6 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 7 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 8 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 9 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 10 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 11 5.544481992908607E-5 0.0 0.0 1.1088963985817215E-4 1.1088963985817215E-4 12 5.544481992908607E-5 0.0 0.0 1.6633445978725823E-4 1.1088963985817215E-4 13 5.544481992908607E-5 0.0 0.0 1.6633445978725823E-4 1.6633445978725823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 840 0.0 13.472107 4 GGTATCA 1760 0.0 12.916999 1 GGACGTA 55 0.0030453529 12.103283 6 TGGACCG 65 7.9559424E-4 11.704273 5 AAATGTC 645 0.0 11.200276 7 GGACGGG 85 5.268537E-5 11.187908 6 AAACGGG 85 5.3707925E-5 11.166816 19 TACACCG 60 0.0058317007 11.094676 5 ACGTGTA 60 0.005872824 11.0842085 8 GGCGAGG 1485 0.0 10.993862 19 AGGACCT 1720 0.0 10.947238 5 TAGAAAT 695 0.0 10.946444 4 GTTCTAA 160 5.329639E-10 10.701113 1 TGTACCG 125 2.2088716E-7 10.650888 5 GTATCAA 3855 0.0 10.634799 1 CGAAATC 250 0.0 10.632282 13 CTAGGAC 260 0.0 10.6078825 3 TGTAGGA 2905 0.0 10.574513 2 AATGTCC 675 0.0 10.556389 8 TAGGACA 695 0.0 10.535952 4 >>END_MODULE