FastQCFastQC Report
Thu 26 May 2016
SRR1512910_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512910_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020353
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT57370.2839602782286066No Hit
GTCCTAAAGTGTGTATTTCTCATTT49490.24495719312417186No Hit
GTATCAACGCAGAGTACTTTTTTTT45990.22763348781128842No Hit
CTGTAGGACGTGGAATATGGCAAGA42820.2119431604279054No Hit
CTTTAGGACGTGAAATATGGCGAGG40560.20075699642587214No Hit
GTCCTACAGTGTGCATTTCTCATTT33810.16734699332245406No Hit
TATCAACGCAGAGTACTTTTTTTTT25820.12779944890818584No Hit
GGTATCAACGCAGAGTACTTTTTTT25420.12581959687242775No Hit
GTACTGGTTCACTATCGGTCAGTCA23820.1179001887293953No Hit
CTGAAGGACCTGGAATATGGCGAGA21600.10691200993093783No Hit
CTGTAGGACCTGGAATATGGCGAGA21180.1048331652933918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8400.012.7777454
GACCGCG550.003072050612.0893017
TGTCGAG1354.7384674E-911.2580418
GGCGAGG10600.011.2013419
GGACCGT855.3316744E-511.1749836
TATGTCG1303.272544E-810.96035216
GTCCTAA14000.010.8837451
GGACCGC1053.472811E-610.8556996
TAGAACG700.001493701510.855434
GTCCTAC27550.010.85411551
GGTATCA17000.010.8117191
AATGTCC5500.010.7076668
GACGTGA13750.010.7076657
TGTAGGA26950.010.6792052
TTAGGAC16250.010.6383223
GGACGTG26350.010.6342586
TCCAACG1701.382432E-1010.61649718
GTCCTAT2900.010.5084441
AGGACGT26850.010.50672155
GCACCGT1451.4468242E-810.4813646
Produced by FastQC (version 0.11.5)