##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512910_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2020353
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.522888821903894	31.0	30.0	34.0	16.0	34.0
2	28.59076062450473	31.0	28.0	34.0	16.0	34.0
3	28.77770221342508	31.0	30.0	34.0	16.0	34.0
4	32.178230734926025	35.0	33.0	37.0	20.0	37.0
5	32.36112996095237	35.0	33.0	37.0	23.0	37.0
6	32.2350811962068	35.0	33.0	37.0	22.0	37.0
7	32.2052047340242	35.0	33.0	37.0	21.0	37.0
8	32.12264044946601	35.0	33.0	37.0	19.0	37.0
9	33.37015313660534	38.0	33.0	39.0	18.0	39.0
10	33.24553580488162	38.0	33.0	39.0	17.0	39.0
11	33.28286195531177	38.0	33.0	39.0	17.0	39.0
12	33.18263046111249	38.0	33.0	39.0	17.0	39.0
13	33.147382660356875	38.0	33.0	39.0	17.0	39.0
14	33.973595208362106	39.0	33.0	41.0	16.0	41.0
15	34.133709802197934	39.0	33.0	41.0	17.0	41.0
16	34.060598816147476	39.0	33.0	41.0	17.0	41.0
17	33.82910313197743	39.0	33.0	41.0	11.0	41.0
18	33.79810260880153	39.0	33.0	41.0	11.0	41.0
19	33.90727907449837	39.0	33.0	41.0	11.0	41.0
20	33.8926469780281	39.0	33.0	41.0	10.0	41.0
21	33.79477942715951	39.0	33.0	41.0	10.0	41.0
22	33.780360659746094	39.0	33.0	41.0	2.0	41.0
23	33.42477428449385	39.0	33.0	41.0	2.0	41.0
24	33.481007526902474	39.0	33.0	41.0	2.0	41.0
25	33.17881726609162	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6529809570675589
1101	2	0.3184425308122627
1101	3	0.6379514068918866
1101	4	0.7165551947129245
1101	5	0.531079077658795
1101	6	0.5992765848757386
1101	7	0.2959805317768769
1101	8	0.6082232103900651
1101	9	0.6969187543530424
1101	10	0.626958300635934
1101	11	0.6332467903566226
1101	12	0.5022828601748728
1101	13	0.9205552095895371
1101	14	1.1576038523573544
1101	15	0.899955167318268
1101	16	0.7999685004118717
1101	17	0.13641197577815944
1101	18	0.5457586428607826
1101	19	0.21477764887643502
1101	20	-0.5943171139769206
1101	21	0.5836204994007659
1101	22	0.5418302091932858
1101	23	-0.022520899880134948
1101	24	0.6550945009595068
1101	25	0.26223343323677994
1102	1	1.0646262895683378
1102	2	1.1013461799268462
1102	3	1.0217951426861234
1102	4	1.1780236255181151
1102	5	1.056255879955465
1102	6	1.0153501870380524
1102	7	1.0572578137065136
1102	8	0.8642497675121703
1102	9	1.2283463293580539
1102	10	1.2598608232293103
1102	11	0.9457741605619745
1102	12	1.081532964354416
1102	13	1.1073003338826908
1102	14	1.2986754523412216
1102	15	1.3858530151326391
1102	16	1.3457226798528765
1102	17	1.512551347845779
1102	18	1.5794632106865407
1102	19	1.2463568782488323
1102	20	1.5687172002376712
1102	21	1.5954461320938904
1102	22	1.3839535634546678
1102	23	1.2907856632702774
1102	24	0.6808073001526509
1102	25	1.0799057867213335
1103	1	0.4545179863192388
1103	2	0.5493088440948242
1103	3	0.8014320374529618
1103	4	0.888667643443867
1103	5	0.5166291580024023
1103	6	0.15646988252501615
1103	7	0.06723833648989341
1103	8	0.27452394454686413
1103	9	0.4197366187724114
1103	10	0.6208490310845605
1103	11	0.437650322244032
1103	12	0.46329808619830715
1103	13	0.595630058567231
1103	14	0.7309705967564994
1103	15	0.6608121669797669
1103	16	0.5903306871854781
1103	17	0.22834124786175636
1103	18	0.7168558542972647
1103	19	0.5737394074263449
1103	20	0.7935356549186636
1103	21	0.6444670170119267
1103	22	0.5443828138700795
1103	23	-0.14970282882536168
1103	24	0.10185141469823833
1103	25	0.5165075925782219
1104	1	0.8929258969377116
1104	2	0.7742588793672489
1104	3	0.871011850358272
1104	4	1.11991138081639
1104	5	0.7387205094069884
1104	6	0.6668273642954787
1104	7	0.8309176230891708
1104	8	0.6242545039155729
1104	9	1.0482166588677657
1104	10	1.0801454506697894
1104	11	0.8323637651272335
1104	12	1.169721920109339
1104	13	1.1357219595020496
1104	14	1.3229806562126214
1104	15	1.0433528974551791
1104	16	1.1563655394683323
1104	17	1.3402900412394843
1104	18	1.097689297572984
1104	19	0.5736325543264655
1104	20	0.7625100941306968
1104	21	0.9626985364230265
1104	22	0.721505183566542
1104	23	1.2250677913696606
1104	24	1.0670275714829955
1104	25	0.5397765814462332
1105	1	0.5985806164914145
1105	2	0.6685248604791596
1105	3	0.7482581505696899
1105	4	0.8754790326413158
1105	5	0.618437177186884
1105	6	0.8445205831405858
1105	7	0.855464841858776
1105	8	0.7891820545626018
1105	9	0.49132986833271985
1105	10	0.7476537236468133
1105	11	0.73559815791468
1105	12	0.8496729647042756
1105	13	0.7571274435528892
1105	14	0.8826537229714404
1105	15	0.8748615051265745
1105	16	0.8147305211436375
1105	17	1.140424046042817
1105	18	0.9054015707649086
1105	19	0.41766080078929946
1105	20	0.7805433871458192
1105	21	0.7456907077691284
1105	22	0.7454782127176216
1105	23	0.9871439238127948
1105	24	1.0829952520582324
1105	25	1.254438100886098
1106	1	0.5362487448428581
1106	2	0.6264545869063411
1106	3	0.45665574464742065
1106	4	0.6150031719427886
1106	5	0.43589186594738294
1106	6	0.6243690192317928
1106	7	0.512961623878418
1106	8	0.6338592629572233
1106	9	0.7158878038426195
1106	10	0.39147724040996934
1106	11	0.12605465882817413
1106	12	0.4521815805871583
1106	13	0.508339005741469
1106	14	0.7834290782839304
1106	15	0.5890040097533458
1106	16	0.6723929105162938
1106	17	0.8648505559725876
1106	18	0.6999394569225501
1106	19	0.6537230160647738
1106	20	0.701273001166129
1106	21	0.7061927794366625
1106	22	0.6472413916076718
1106	23	0.9840933303401087
1106	24	0.5493970161639581
1106	25	0.7445856311973955
1107	1	0.7045134294554778
1107	2	0.628703617532917
1107	3	0.6389434787550172
1107	4	0.7114072513820346
1107	5	0.5379489365423922
1107	6	0.7119946420208905
1107	7	0.4009453326517658
1107	8	0.6504991580672055
1107	9	0.5574170750449312
1107	10	0.7615721320964681
1107	11	0.6826404526132279
1107	12	0.717811793780605
1107	13	0.5242699294453743
1107	14	0.770877197132414
1107	15	0.46258796785303957
1107	16	0.662020161402026
1107	17	1.0609590642340834
1107	18	0.9026775211658631
1107	19	0.8439003129663121
1107	20	0.864797498743684
1107	21	0.7320460964682667
1107	22	0.7935330928037558
1107	23	1.1113097706348896
1107	24	0.8639992986872826
1107	25	1.0275565575983023
1108	1	0.6649518296689578
1108	2	0.670520714395888
1108	3	0.6090712609573785
1108	4	0.792038217482073
1108	5	0.650170133995907
1108	6	0.7320691900622975
1108	7	0.7065531887183383
1108	8	0.6933623921112613
1108	9	0.8643525110126262
1108	10	0.9371653174558574
1108	11	0.5100365194292422
1108	12	0.7132283733936973
1108	13	0.6424250680207564
1108	14	0.7507308381284048
1108	15	0.7888523308146489
1108	16	0.5269351269384757
1108	17	0.8766569758183564
1108	18	0.9660721862178221
1108	19	0.9399255454224402
1108	20	0.9244098267786214
1108	21	0.864205427503002
1108	22	0.9600754599971992
1108	23	1.2214446706484523
1108	24	1.120464021266237
1108	25	1.3330805609619034
1109	1	0.8126836715169325
1109	2	0.8298318458578002
1109	3	0.9365800274375928
1109	4	1.120465511904726
1109	5	0.882287849918967
1109	6	0.9196438287495781
1109	7	0.9562694047122378
1109	8	0.816455219420753
1109	9	0.9400423091345971
1109	10	1.045395681610394
1109	11	0.9659339995050971
1109	12	0.9283924604282205
1109	13	1.0493204754164225
1109	14	1.1708713565408004
1109	15	1.029150208055448
1109	16	0.6019608607569538
1109	17	1.073907158772137
1109	18	1.314775996025844
1109	19	1.1658306160681633
1109	20	1.0698923586502573
1109	21	1.0586327180339623
1109	22	1.072431283835833
1109	23	1.2704534653434436
1109	24	1.1455922392760343
1109	25	0.6838955256527512
1110	1	0.5173330117575112
1110	2	0.5123170457626465
1110	3	0.4136730821580308
1110	4	0.48237046345172985
1110	5	0.3041848791614399
1110	6	0.3637604686826137
1110	7	0.23546236794214792
1110	8	0.2927960463486201
1110	9	0.48041245996773796
1110	10	0.3530195129549156
1110	11	0.422754061852352
1110	12	0.45396678305873905
1110	13	0.46621343861077236
1110	14	0.5433185201388824
1110	15	0.36631004766078235
1110	16	0.5335564904041448
1110	17	0.5403310596679489
1110	18	-0.16251316062208332
1110	19	0.3894499287122386
1110	20	0.44217826708221253
1110	21	0.6183778459766955
1110	22	0.6234524539545205
1110	23	0.6330297504155524
1110	24	0.5882712725130901
1110	25	0.7373317896816829
1111	1	0.4672570727555154
1111	2	0.41567070205065093
1111	3	0.5423880265902525
1111	4	0.4902823126883007
1111	5	0.6691926223672695
1111	6	0.4760830940440286
1111	7	0.27386800327627725
1111	8	0.532212390853914
1111	9	0.6524657019955811
1111	10	0.5097889852422597
1111	11	0.299859518577378
1111	12	-0.11392788343835747
1111	13	0.27926507119587995
1111	14	0.3255710995735228
1111	15	0.31676504307638
1111	16	0.4074878533468862
1111	17	-0.05794514387483929
1111	18	0.26552818156716995
1111	19	0.2087789636024482
1111	20	0.4332431346220389
1111	21	0.40067872488317136
1111	22	-0.04037476741194013
1111	23	0.4775846010766429
1111	24	0.45729488744703417
1111	25	0.5059877529935122
1112	1	0.3718443346811213
1112	2	0.25244800969836234
1112	3	0.3441416092163081
1112	4	0.46461563616936274
1112	5	0.29473570596073984
1112	6	0.1362317420474639
1112	7	-0.026655061264420965
1112	8	0.14059779342899503
1112	9	0.13562100482780437
1112	10	0.13039835565953695
1112	11	0.04528719420733296
1112	12	0.11005414602296071
1112	13	0.12325887790800749
1112	14	0.10223386322592631
1112	15	-0.13547684073808597
1112	16	0.013323367442076517
1112	17	0.39468798112089587
1112	18	0.14922278244796416
1112	19	0.2719777831490475
1112	20	-0.08663263584434588
1112	21	0.10603637361643337
1112	22	0.04928848961178289
1112	23	0.1567201781505645
1112	24	0.35254665460306
1112	25	0.2173972716495456
1113	1	0.06849266754544914
1113	2	0.20618077960643078
1113	3	0.14775165505468735
1113	4	0.17185233292207158
1113	5	0.10382719434765164
1113	6	-0.24082199653654257
1113	7	-0.22048364757709038
1113	8	-0.051270928794131976
1113	9	0.04246531944981058
1113	10	-0.009490246141908187
1113	11	0.002226092644271205
1113	12	-0.1903408873383583
1113	13	-0.03390597979765886
1113	14	0.2345329685518749
1113	15	-0.05519571726748751
1113	16	-0.14830532538495333
1113	17	0.12182630326402233
1113	18	-0.10665892615388373
1113	19	-0.6051893071718695
1113	20	-0.32217094031260274
1113	21	-0.037360410952473444
1113	22	-0.7444618621414065
1113	23	-4.424970795339455
1113	24	-1.852714689291787
1113	25	-0.8186902561709317
1114	1	-0.4832460508571934
1114	2	-0.7367022262638834
1114	3	-0.566568977089883
1114	4	-0.622820374068997
1114	5	-0.5487132422075511
1114	6	-1.1822222053209543
1114	7	-1.0571655795814863
1114	8	-0.8314577776796277
1114	9	-1.0951318747114698
1114	10	-0.769269097547955
1114	11	-0.9696174210542239
1114	12	-1.8779891670469517
1114	13	-1.1957929737957222
1114	14	-3.1803705200727457
1114	15	-1.6766677501925713
1114	16	-1.7352707574979362
1114	17	-1.3725605591126637
1114	18	-1.3712197467473644
1114	19	-1.466507059276168
1114	20	-1.0220151746381418
1114	21	-0.9472626985320218
1114	22	-1.02323470667681
1114	23	-5.710092931751852
1114	24	-2.6828854762560077
1114	25	-2.5848174739911514
1115	1	-1.7794743564704234
1115	2	-1.4990246876860382
1115	3	-1.2951582044116101
1115	4	-1.5001014949788072
1115	5	-1.349483103558807
1115	6	-1.4100836895100066
1115	7	-2.0412639738894
1115	8	-2.493716349630116
1115	9	-3.79783173617124
1115	10	-4.364980997176822
1115	11	-3.228233908788166
1115	12	-3.3988825616080725
1115	13	-2.6998853137510146
1115	14	-3.4256048085981448
1115	15	-2.9783771891950863
1115	16	-3.237484168228111
1115	17	-3.0098111279233883
1115	18	-5.660047861008689
1115	19	-3.535106401300517
1115	20	-3.735769316376004
1115	21	-3.4412305014938163
1115	22	-2.89246023786826
1115	23	-3.3125857967492855
1115	24	-3.1821066396321918
1115	25	-3.500998036580228
1116	1	-0.5866152454203757
1116	2	-0.2821528830527029
1116	3	-0.9764878990917083
1116	4	-0.4753400657264599
1116	5	-0.3998663748620821
1116	6	-3.0157083791358517
1116	7	-1.3444491514560362
1116	8	-1.6213506442566405
1116	9	-2.615695548406297
1116	10	-1.9385948383865284
1116	11	-1.4242650724638573
1116	12	-1.1963921616197375
1116	13	-2.594414707862292
1116	14	-5.7791650064086575
1116	15	-3.5763235964535625
1116	16	-2.1779690849077618
1116	17	-3.5807345481141546
1116	18	-2.014688180059885
1116	19	-1.8423520595163083
1116	20	-2.350116910865985
1116	21	-3.192727013406426
1116	22	-2.7040647580672363
1116	23	-1.4698412027282686
1116	24	-2.688056037228101
1116	25	-2.669779021227118
1201	1	0.4489695685594164
1201	2	0.5251765859837398
1201	3	0.8056827891424376
1201	4	0.7644054058874161
1201	5	0.4689786341054685
1201	6	0.8532716811755066
1201	7	0.9648928950613396
1201	8	0.9177798750557002
1201	9	1.2154802037324828
1201	10	1.1085882474915465
1201	11	1.3377857429476876
1201	12	1.2215679659464982
1201	13	1.2379846649995443
1201	14	1.447403585439794
1201	15	1.41204237255198
1201	16	1.331979928870254
1201	17	1.5613123858731655
1201	18	1.3138221307109603
1201	19	1.5233211523379353
1201	20	1.4800469977212671
1201	21	1.6824792957479744
1201	22	1.468642997073914
1201	23	1.8698221271171036
1201	24	1.6813647626230264
1201	25	1.7996674349075832
1202	1	0.7906578429663966
1202	2	0.7018403011064933
1202	3	0.8110615486279436
1202	4	0.9275759799900811
1202	5	0.7066414107567098
1202	6	0.881339687882928
1202	7	0.9077951723292728
1202	8	0.8308117689016541
1202	9	1.1728202529481067
1202	10	1.036794990450396
1202	11	1.1752131924553808
1202	12	1.2997698186997937
1202	13	1.2175223277948604
1202	14	1.7232005396256653
1202	15	1.3223736410489053
1202	16	1.0631405986268305
1202	17	1.4025278781936805
1202	18	1.458251178681266
1202	19	1.4356954815211935
1202	20	1.3016266349053467
1202	21	1.4863641347668874
1202	22	1.5517498006563812
1202	23	1.4552783758292236
1202	24	1.6486575933577114
1202	25	1.9783312814312808
1203	1	1.0484183384980028
1203	2	1.083976371888241
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2211	19	0.1284579747447907
2211	20	-0.0836072742169236
2211	21	0.1890371178628385
2211	22	0.1793587159390171
2211	23	0.38858470622201224
2211	24	-0.09409011498588882
2211	25	0.44359913417387276
2212	1	0.042257039410806385
2212	2	0.12314033597719742
2212	3	-0.19900870513289348
2212	4	0.0852456611372645
2212	5	-0.19434777779590462
2212	6	-0.35903970689625453
2212	7	-0.12515859798902795
2212	8	-0.025832744162620713
2212	9	0.05321565307413323
2212	10	-0.25493094111737946
2212	11	-0.5282885220208513
2212	12	0.07117935981357704
2212	13	0.05534220531915679
2212	14	0.0071035042471905285
2212	15	-0.23233761270581255
2212	16	-0.3906394697075086
2212	17	-0.043061780807555294
2212	18	0.10091211921142218
2212	19	-0.14377473614541003
2212	20	-0.027215757163709497
2212	21	0.21985560979762653
2212	22	0.2051560412887028
2212	23	0.21719234154581812
2212	24	0.33334136349247956
2212	25	0.49264891683054657
2213	1	-0.3949696700276206
2213	2	-0.2134074433281441
2213	3	-0.48217114235960423
2213	4	-0.30900401902923846
2213	5	-0.2361695875761285
2213	6	-0.10977902350277446
2213	7	-0.35463495417852187
2213	8	-0.32159518902714623
2213	9	-0.8312832824054297
2213	10	-0.32556342378908454
2213	11	-0.39449787452510066
2213	12	-0.4882070372382259
2213	13	-0.5110835121580664
2213	14	-0.5825572813020585
2213	15	-0.48751223224716256
2213	16	-0.4833130151811531
2213	17	-0.5200964230714007
2213	18	-0.6195474385590956
2213	19	-0.4291789913629884
2213	20	-0.3042255765293902
2213	21	-0.5048465154243473
2213	22	-0.7392579712373077
2213	23	-0.5861339268088059
2213	24	-0.28123600279802474
2213	25	-0.1054862629445168
2214	1	-2.5480450578071228
2214	2	-1.2227615789673827
2214	3	-1.353568161700455
2214	4	-2.9752628966722696
2214	5	-1.5492426451326367
2214	6	-1.2142505821180123
2214	7	-0.9925686416044357
2214	8	-0.8323357440254746
2214	9	-0.6795911479211654
2214	10	-0.6117657808603028
2214	11	-0.5963635726295138
2214	12	-0.4141604023841694
2214	13	-0.9158441957239205
2214	14	-0.7905841396548965
2214	15	-1.216454108380617
2214	16	-0.8774293920896525
2214	17	-3.281609181042281
2214	18	-3.2104166370637017
2214	19	-1.9036880615027023
2214	20	-1.6042123423825032
2214	21	-1.551497833013478
2214	22	-2.7448805840251715
2214	23	-1.638239739195459
2214	24	-2.804546781092501
2214	25	-2.7274158142246208
2215	1	-1.9217753840718288
2215	2	-3.501459247985597
2215	3	-2.1073032162364456
2215	4	-3.6843262792887366
2215	5	-2.3153610841316414
2215	6	-2.057243997510632
2215	7	-2.1614333249151763
2215	8	-1.562355300603155
2215	9	-3.8504538056293
2215	10	-2.2312032424780703
2215	11	-1.944157652218351
2215	12	-1.898465479400432
2215	13	-2.071669761682603
2215	14	-1.7301049335568237
2215	15	-1.9460938644689634
2215	16	-2.0813923968640893
2215	17	-2.404474989066074
2215	18	-1.8510438884132903
2215	19	-1.9112763773568204
2215	20	-1.6331859438004273
2215	21	-2.399974574255335
2215	22	-2.2917348847236383
2215	23	-2.286928483251895
2215	24	-2.3228775754280875
2215	25	-3.1508325078200485
2216	1	-3.7588350709129195
2216	2	-3.6379076805452932
2216	3	-5.4069651256913005
2216	4	-5.9218317409499726
2216	5	-5.46836311002879
2216	6	-4.48564799199379
2216	7	-6.324514657037749
2216	8	-4.017897055447012
2216	9	-4.80470751197554
2216	10	-3.7870636937258446
2216	11	-3.984188160073785
2216	12	-4.540179211549592
2216	13	-4.126467891583982
2216	14	-5.2388442369296015
2216	15	-4.518417069607345
2216	16	-4.734111578089269
2216	17	-4.3985254086701
2216	18	-4.061071950218963
2216	19	-4.719339241481016
2216	20	-5.319478610104383
2216	21	-5.082123800070907
2216	22	-4.724763623549418
2216	23	-4.8156479471604
2216	24	-5.5590580396870415
2216	25	-5.665290985318652
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113061.0
3	2.0
4	970.0
5	5949.0
6	7303.0
7	6408.0
8	5932.0
9	5339.0
10	4920.0
11	4457.0
12	4214.0
13	4092.0
14	4261.0
15	4397.0
16	4495.0
17	4605.0
18	5408.0
19	6562.0
20	8452.0
21	10915.0
22	13582.0
23	16382.0
24	19301.0
25	21483.0
26	24042.0
27	26300.0
28	29474.0
29	34848.0
30	41813.0
31	51644.0
32	65019.0
33	81017.0
34	103953.0
35	133131.0
36	178170.0
37	270131.0
38	698321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.28662813913617	18.42124868136911	12.46684798048327	24.825275199011447
2	14.703548507656627	19.942429875083224	42.15697388058778	23.197047736672367
3	19.407225170283542	25.47100229684777	28.7527720576588	26.369000475209887
4	12.389771727110091	16.22443352543356	37.89976466814446	33.48603007931189
5	12.933703347652742	38.92427214071369	34.90417810585802	13.23784640577555
6	31.84317497996269	36.141066628887216	18.49297629108144	13.522782100068657
7	28.69107162229169	31.423514533548847	21.649957424000977	18.235456420158485
8	25.53284997753005	36.864195781396866	20.498144185237614	17.104810055835472
9	27.903815545239596	14.592529808095755	19.73335816825295	37.7702964784117
10	16.362381607746734	28.459867763321512	33.4525801807479	21.72517044818385
11	34.648021428681126	22.01636478297119	24.76668270085797	18.568931087489716
12	24.62456555827774	24.266607874078165	31.10852930174718	20.00029726589691
13	29.611623552257438	22.00420080647558	25.542934421844194	22.841241219422788
14	21.42474624450789	22.583582394924743	27.059705921547707	28.931965439019663
15	23.274146590846605	31.60267883460094	22.92485184324686	22.1983227313056
16	22.08654573520677	27.661969207508736	28.12887488364512	22.122610173639373
17	21.77863348818036	28.623777210533824	27.60561175146259	21.991977549823222
18	21.62584917056146	26.764845342078676	30.300655058507363	21.308650428852502
19	23.88117748418456	26.400550128094036	27.298917628920172	22.419354758801234
20	24.60007817852944	26.600170328126882	27.803118917073782	20.996632576269896
21	23.64928726831762	26.335207795861916	26.64099683362461	23.374508102195858
22	24.146121644500223	28.162242213833387	26.456838979116494	21.234797162549903
23	22.493727754833493	27.7814385141616	27.658173538061533	22.066660192943377
24	23.772683811326175	26.421964716548345	28.159483975803933	21.645867496321543
25	22.87650765058671	27.1705065745604	28.02865242071242	21.924333354140472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	264.0
1	264.0
2	579.0
3	894.0
4	894.0
5	894.0
6	1459.5
7	2025.0
8	2025.0
9	2025.0
10	2300.0
11	2575.0
12	2575.0
13	2575.0
14	4068.0
15	5561.0
16	5561.0
17	5561.0
18	11241.5
19	16922.0
20	16922.0
21	16922.0
22	29612.5
23	42303.0
24	42303.0
25	42303.0
26	66683.5
27	91064.0
28	91064.0
29	91064.0
30	120768.0
31	150472.0
32	150472.0
33	150472.0
34	170343.5
35	190215.0
36	190215.0
37	190215.0
38	205620.0
39	221025.0
40	221025.0
41	221025.0
42	235215.5
43	249406.0
44	249406.0
45	249406.0
46	263036.0
47	276666.0
48	276666.0
49	276666.0
50	264565.5
51	252465.0
52	252465.0
53	252465.0
54	222977.0
55	193489.0
56	193489.0
57	193489.0
58	167195.0
59	140901.0
60	140901.0
61	140901.0
62	116978.5
63	93056.0
64	93056.0
65	93056.0
66	71626.5
67	50197.0
68	50197.0
69	50197.0
70	37354.5
71	24512.0
72	24512.0
73	24512.0
74	17528.5
75	10545.0
76	10545.0
77	10545.0
78	7270.5
79	3996.0
80	3996.0
81	3996.0
82	2660.5
83	1325.0
84	1325.0
85	1325.0
86	832.5
87	340.0
88	340.0
89	340.0
90	223.0
91	106.0
92	106.0
93	106.0
94	65.0
95	24.0
96	24.0
97	24.0
98	14.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34157397246916754
2	0.01029523058594216
3	0.00955278607253287
4	0.01123566030292726
5	0.012423571524382124
6	0.0801345111473094
7	0.07968904443926383
8	0.10404122447908856
9	0.08364874851078005
10	0.09161765295470643
11	0.08661852656441721
12	0.09691375715035937
13	0.08379723741346191
14	0.08790543038765998
15	0.08483665973223492
16	0.0863215487590535
17	0.09983403890310258
18	0.10196237984154255
19	0.09473591991102545
20	0.0938449864949343
21	0.08122342976697636
22	0.10206137244333044
23	0.09587433483158636
24	0.0912216825475548
25	0.08983578612252413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2020353.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.93225889592709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92374725362318	42.46586687738585
2	13.465466481604013	15.063079148070083
3	4.666654172241989	7.83049528018792
4	2.184522674126763	4.887411510931243
5	1.1468593407000172	3.2073216780622813
6	0.6801301715459762	2.282473010270451
7	0.42122902951783187	1.6492203793430045
8	0.29955177439348774	1.340368591848872
9	0.20833441310202724	1.0487352897498252
>10	0.9033723277624895	8.613732285094123
>50	0.0541818537023419	2.139117612936066
>100	0.03811622811265994	4.2615639486226105
>500	0.0052525283461403085	2.106892156797927
>1k	0.0024927253168123436	2.8180529098558726
>5k	8.902590417186941E-5	0.2856693208439612
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5737	0.2839602782286066	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4949	0.24495719312417186	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4599	0.22763348781128842	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4282	0.2119431604279054	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4056	0.20075699642587214	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3381	0.16734699332245406	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2582	0.12779944890818584	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2542	0.12581959687242775	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2382	0.1179001887293953	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2160	0.10691200993093783	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2118	0.1048331652933918	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.949630089395269E-5	0.0
3	0.0	0.0	0.0	4.949630089395269E-5	0.0
4	0.0	0.0	0.0	4.949630089395269E-5	0.0
5	0.0	0.0	0.0	4.949630089395269E-5	0.0
6	0.0	0.0	0.0	4.949630089395269E-5	0.0
7	0.0	0.0	0.0	4.949630089395269E-5	0.0
8	0.0	0.0	0.0	4.949630089395269E-5	0.0
9	0.0	0.0	0.0	4.949630089395269E-5	0.0
10	0.0	0.0	0.0	9.899260178790539E-5	0.0
11	0.0	0.0	0.0	9.899260178790539E-5	0.0
12	0.0	0.0	0.0	9.899260178790539E-5	0.0
13	0.0	0.0	0.0	9.899260178790539E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	840	0.0	12.777745	4
GACCGCG	55	0.0030720506	12.089301	7
TGTCGAG	135	4.7384674E-9	11.25804	18
GGCGAGG	1060	0.0	11.20134	19
GGACCGT	85	5.3316744E-5	11.174983	6
TATGTCG	130	3.272544E-8	10.960352	16
GTCCTAA	1400	0.0	10.883745	1
GGACCGC	105	3.472811E-6	10.855699	6
TAGAACG	70	0.0014937015	10.85543	4
GTCCTAC	2755	0.0	10.8541155	1
GGTATCA	1700	0.0	10.811719	1
AATGTCC	550	0.0	10.707666	8
GACGTGA	1375	0.0	10.707665	7
TGTAGGA	2695	0.0	10.679205	2
TTAGGAC	1625	0.0	10.638322	3
GGACGTG	2635	0.0	10.634258	6
TCCAACG	170	1.382432E-10	10.616497	18
GTCCTAT	290	0.0	10.508444	1
AGGACGT	2685	0.0	10.5067215	5
GCACCGT	145	1.4468242E-8	10.481364	6
>>END_MODULE