FastQCFastQC Report
Thu 26 May 2016
SRR1512910_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512910_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2020353
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59750.29574039784136735No Hit
GTCCTACAGTGGACATTTCTAAATT52780.2612414761182823No Hit
GTCCTAAAGTGTGTATTTCTCATTT45760.2264950728907275No Hit
CTTTAGGACGTGAAATATGGCGAGG44360.21956559076557414No Hit
TATCAACGCAGAGTACTTTTTTTTT42550.2106067603037687No Hit
CTGTAGGACGTGGAATATGGCAAGA42370.20971582688767754No Hit
GGTATCAACGCAGAGTACTTTTTTT33430.16546613388848383No Hit
GTCCTACAGTGTGCATTTCTCATTT32850.1625953484366346No Hit
CTGAAGGACCTGGAATATGGCGAGA23400.11582134409184928No Hit
GTACTGGTTCACTATCGGTCAGTCA22050.10913934347116569No Hit
CTGTAGGACCTGGAATATGGCGAGA21990.10884236566580197No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20420.10107144642545139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA954.971298E-913.98648619
CCATCGC957.4847776E-812.98745219
GCGCCAC1700.012.84339513
TAGGACC8300.012.8351754
CGATACT802.831213E-511.8897284
GGCGAGG12650.011.8541619
ATGTCCA8050.011.4426699
TCCAACG1850.011.28695218
GCACCGT855.260313E-511.1897756
ACTATCC855.324456E-511.176478
TAGAAAT7850.011.1475664
GTATTAT2050.011.1357441
CGTCTCG600.005909276711.07510212
ACGGTTT600.005909276711.07510212
TTTAGAA8350.010.9367872
TGGCGAG26400.010.89342318
TAAGACG700.00147639210.8706074
AATGTCC8250.010.8242448
ATTTAGA7600.010.7633311
AAATGTC7950.010.7619157
Produced by FastQC (version 0.11.5)