FastQCFastQC Report
Thu 26 May 2016
SRR1512909_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512909_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776511
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57510.32372442388479444No Hit
TATCAACGCAGAGTACTTTTTTTTT33000.18575736373149393No Hit
GTCCTACAGTGGACATTTCTAAATT32970.18558849340082892No Hit
GGTATCAACGCAGAGTACTTTTTTT32160.18102899447287407No Hit
GTCCTAAAGTGTGTATTTCTCATTT29660.16695646691745786No Hit
CTGTAGGACGTGGAATATGGCAAGA27400.15423490200736162No Hit
GTACTGGTTCACTATCGGTCAGTCA26070.14674831734788019No Hit
CTTTAGGACGTGAAATATGGCGAGG22430.1262587172271942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14700.012.9569141
TACCGTC604.0945318E-412.6659357
CCGCCCC550.003072661612.0888489
TAGGACC4850.011.947094
AGGACCG1950.011.6909725
TATACCG658.0320984E-411.6909725
ACGAAAT1302.6211637E-911.69097212
CGAAATC1302.6229827E-911.69031313
GACTAGT600.005882445311.08175611
CCGTTGG600.00588367911.08144413
GTATCAA32750.011.079051
CCTATAC1303.2718162E-810.9602863
TAAGGCG700.00149300410.8559045
CTTACGG700.00149300410.8559043
CCTAATA1355.696711E-810.554352
GGACCGT1002.4004301E-510.4493966
CCTTATA1659.313226E-1010.36245252
GATATAC3050.010.3039321
ATTTCTA11550.010.19739515
GTCCTAC14950.010.1921941
Produced by FastQC (version 0.11.5)