##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512909_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776511
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.831895777735124	31.0	30.0	34.0	22.0	34.0
2	28.90188633788364	31.0	30.0	34.0	18.0	34.0
3	29.06557234939722	31.0	30.0	34.0	22.0	34.0
4	32.487432951442464	35.0	33.0	37.0	23.0	37.0
5	32.674487802214564	35.0	33.0	37.0	25.0	37.0
6	32.54085451764723	36.0	33.0	37.0	24.0	37.0
7	32.513195809088714	36.0	33.0	37.0	23.0	37.0
8	32.41907030128156	37.0	33.0	37.0	22.0	37.0
9	33.70486419729458	38.0	34.0	39.0	21.0	39.0
10	33.57983147866802	38.0	34.0	39.0	19.0	39.0
11	33.63657979038689	38.0	34.0	39.0	20.0	39.0
12	33.5380017348612	38.0	34.0	39.0	18.0	39.0
13	33.5030585231389	38.0	34.0	39.0	18.0	39.0
14	34.34338881098963	39.0	34.0	41.0	17.0	41.0
15	34.515155830726634	39.0	34.0	41.0	17.0	41.0
16	34.4619723716881	39.0	34.0	41.0	17.0	41.0
17	34.21530010227913	39.0	33.0	41.0	17.0	41.0
18	34.21680023371654	39.0	34.0	41.0	17.0	41.0
19	34.33213472925301	39.0	34.0	41.0	18.0	41.0
20	34.303480248644675	39.0	34.0	41.0	18.0	41.0
21	34.204301577642916	39.0	34.0	41.0	15.0	41.0
22	34.205998161565	39.0	34.0	41.0	11.0	41.0
23	33.877255474353944	39.0	34.0	41.0	8.0	41.0
24	33.933661542202664	39.0	34.0	41.0	2.0	41.0
25	33.64627801347698	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6215982457232023
1101	2	0.2985924794434922
1101	3	0.6727508239333524
1101	4	0.7987481150801727
1101	5	0.7481331966583937
1101	6	0.6191179710030852
1101	7	0.2835916337241713
1101	8	0.7337570288036446
1101	9	0.7843779073122192
1101	10	0.7148937337909729
1101	11	0.668985530592245
1101	12	0.5285512516764754
1101	13	1.0455190906456053
1101	14	1.1934715671140808
1101	15	1.040375358133197
1101	16	0.7551800812592546
1101	17	0.1442194048054759
1101	18	0.5958159302108115
1101	19	-0.06826098323775653
1101	20	-0.548436092820566
1101	21	0.4788550136872871
1101	22	0.5440201771095232
1101	23	0.10236127877099932
1101	24	0.5138212998205418
1101	25	0.17781207707941604
1102	1	0.9851892939231135
1102	2	0.9904408402551788
1102	3	0.9714413214790518
1102	4	1.2393709577170071
1102	5	1.1124488470881033
1102	6	0.9064502062895272
1102	7	0.9064921626348763
1102	8	1.0553369117148819
1102	9	1.0996538051829887
1102	10	1.013176268865962
1102	11	0.8274097789465387
1102	12	1.1260651539285647
1102	13	1.0419151050615483
1102	14	1.2034569989672264
1102	15	1.2373554573922902
1102	16	1.0871221022091149
1102	17	1.30875575178829
1102	18	1.3307775801078066
1102	19	1.024801752203608
1102	20	1.343738692326447
1102	21	1.4033314029612214
1102	22	1.2646979764496038
1102	23	1.2814279932733754
1102	24	0.6926754715833425
1102	25	0.8595991755720505
1103	1	0.7512573019900799
1103	2	0.8318376235946978
1103	3	0.9554951406305037
1103	4	1.051803979116407
1103	5	0.9533388081033891
1103	6	0.6363434413453888
1103	7	0.5604242811416427
1103	8	0.7438489741916783
1103	9	0.9274781925661131
1103	10	1.1935032299150024
1103	11	0.908357373201774
1103	12	1.0536043284990555
1103	13	1.1716663665797569
1103	14	1.2151413367334527
1103	15	1.1396009809012497
1103	16	1.223795892164226
1103	17	0.8661632685804079
1103	18	1.2123631059794775
1103	19	1.12083377113904
1103	20	1.2857494637511593
1103	21	1.1218134885588142
1103	22	1.1221511652638654
1103	23	0.38442947840518116
1103	24	0.6549939437540004
1103	25	1.016899210168873
1104	1	1.0124718159226163
1104	2	0.953778226988792
1104	3	1.0707020335699546
1104	4	1.2017585412366643
1104	5	0.93339891181175
1104	6	0.8884630464155734
1104	7	0.9905639993482254
1104	8	0.738767657409781
1104	9	1.0513401504542372
1104	10	1.0622811211783585
1104	11	0.7818613277377082
1104	12	1.1147887251049653
1104	13	1.1071913246773946
1104	14	1.3197677509383539
1104	15	1.253516364307913
1104	16	1.1317708293106747
1104	17	1.2844562708850376
1104	18	1.1321880326503333
1104	19	0.7372051570598401
1104	20	0.9579856097809127
1104	21	0.9789177730758922
1104	22	0.9255527208892573
1104	23	1.2273071234921318
1104	24	1.1067558073617931
1104	25	0.7720402378244344
1105	1	0.7013000653939514
1105	2	0.6217797333182702
1105	3	0.7687803047671977
1105	4	0.934523331750654
1105	5	0.6138284088328874
1105	6	0.8661102756383698
1105	7	0.8109481133657752
1105	8	0.8294737976336037
1105	9	0.5847889690477501
1105	10	0.711535391525878
1105	11	0.8556328484545688
1105	12	0.9206993302438633
1105	13	0.8663840865711165
1105	14	1.0901417261997892
1105	15	1.0851469659706723
1105	16	0.9567693326371511
1105	17	1.323995488559305
1105	18	0.9792855481722853
1105	19	0.7840867653562427
1105	20	0.901099656303316
1105	21	0.8558773049550368
1105	22	0.8972827789215643
1105	23	1.0688713887219876
1105	24	1.2297495406449173
1105	25	1.3989652979327758
1106	1	0.7799629036612465
1106	2	0.7476046125297735
1106	3	0.6548272873275351
1106	4	0.8798279174931594
1106	5	0.7287723413024025
1106	6	0.901990399760912
1106	7	0.839745776684353
1106	8	0.7761293000863105
1106	9	0.9919503492952302
1106	10	0.7587476350165545
1106	11	0.4695566677810632
1106	12	0.6826077709436689
1106	13	0.8790719348106393
1106	14	1.0548765182422457
1106	15	0.8761265828263944
1106	16	0.9163118027559065
1106	17	1.1272731685981867
1106	18	1.0653161953962567
1106	19	1.1030253638702234
1106	20	1.1171026153922696
1106	21	0.9108272435219433
1106	22	1.019648884763356
1106	23	1.2581426623436869
1106	24	0.9293613032836348
1106	25	1.2106191297851936
1107	1	0.6653962546670122
1107	2	0.6659786758675352
1107	3	0.6646747362097898
1107	4	0.7186343703106814
1107	5	0.6954731842698365
1107	6	0.7132122338444589
1107	7	0.48025952879253
1107	8	0.5458837057679773
1107	9	0.5650237852404558
1107	10	0.8166848480035824
1107	11	0.6621571853538697
1107	12	0.7927906488710192
1107	13	0.49792505910966156
1107	14	0.7501176123140354
1107	15	0.485456916164722
1107	16	0.5545078580351444
1107	17	0.8598370948352922
1107	18	0.8686726959598374
1107	19	0.7626931712429013
1107	20	0.9085065983200451
1107	21	0.655870049846726
1107	22	0.7667714832664245
1107	23	1.0948779602046272
1107	24	0.8443635476910956
1107	25	1.0766380386957835
1108	1	0.7664381382659009
1108	2	0.7370280736941339
1108	3	0.7703369564509508
1108	4	0.8949635787348953
1108	5	0.7594750402962589
1108	6	0.8389446274730759
1108	7	0.8362731640102652
1108	8	0.7989157636311859
1108	9	0.9340494566190429
1108	10	0.9539862294279686
1108	11	0.810246781254051
1108	12	0.8288512693245735
1108	13	0.9715637599398761
1108	14	1.0328699630981575
1108	15	1.0111089306962882
1108	16	0.7716104595167934
1108	17	1.1547925757763124
1108	18	1.1133738257733015
1108	19	0.9972683616256646
1108	20	1.0844815297846822
1108	21	0.9866966514306839
1108	22	1.0456300757310117
1108	23	1.299041475645332
1108	24	1.1458388694815014
1108	25	1.245465076275451
1109	1	0.9439013322727092
1109	2	0.9424930837445942
1109	3	1.0712524321860073
1109	4	1.112578955721041
1109	5	1.0124712351057497
1109	6	1.0691491048707817
1109	7	1.0080777927038795
1109	8	0.9337820152197622
1109	9	1.0590344101922682
1109	10	1.135484391515412
1109	11	1.118257024528745
1109	12	1.0685941648546304
1109	13	1.154180255012598
1109	14	1.2901179670210112
1109	15	1.1112139630715205
1109	16	0.9823107020460995
1109	17	1.3192673615379888
1109	18	1.3604723141079305
1109	19	1.266015223744951
1109	20	1.2412104088828713
1109	21	1.1405156717157965
1109	22	1.1934166031265931
1109	23	1.5292724421464712
1109	24	1.326532378850331
1109	25	1.0380754788543811
1110	1	0.4759014739543481
1110	2	0.48765459470599026
1110	3	0.38289817434741025
1110	4	0.4347601934078682
1110	5	0.37029407687300875
1110	6	0.4774477404521704
1110	7	0.43891089295463104
1110	8	0.540179796192163
1110	9	0.7345029672502719
1110	10	0.5606838145291491
1110	11	0.6422123361856009
1110	12	0.6555836376645843
1110	13	0.5645696718827296
1110	14	0.5729371140731203
1110	15	0.6045460098704325
1110	16	0.625450806866084
1110	17	0.6732838527922027
1110	18	0.015160693892887878
1110	19	0.42864815715336135
1110	20	0.6721618621578571
1110	21	0.6445769270395445
1110	22	0.6701220653201929
1110	23	0.8871674682659645
1110	24	0.7296773448162028
1110	25	0.9484210477000516
1111	1	0.19125788745824934
1111	2	0.13011940178881076
1111	3	0.31789535649007306
1111	4	0.12179198974646965
1111	5	0.22192449518847468
1111	6	0.07414266649099233
1111	7	0.1046972569490805
1111	8	0.23600815535080244
1111	9	0.34150155529165005
1111	10	0.32003092001397704
1111	11	-0.009631390900374015
1111	12	-0.20284842167404094
1111	13	0.13460584953499932
1111	14	0.18056956851775396
1111	15	0.2339414128240307
1111	16	0.29145653953928985
1111	17	-0.3711534544269668
1111	18	0.08224751419842846
1111	19	0.07570092077452273
1111	20	0.2450564985330601
1111	21	0.2842414505186568
1111	22	-0.11616072635834485
1111	23	0.3338317917681124
1111	24	0.17571022587838314
1111	25	0.29400549555150945
1112	1	0.3762651958477399
1112	2	0.272383124808826
1112	3	0.2605637102431224
1112	4	0.4236792846850932
1112	5	0.2492166356322798
1112	6	0.3147117758738389
1112	7	0.14380149753316118
1112	8	0.11604563615937735
1112	9	0.13723912581361475
1112	10	0.2954590222749758
1112	11	0.2505665431142603
1112	12	0.42585446498640067
1112	13	0.2574550291667421
1112	14	0.2855155870716146
1112	15	0.2292958298280965
1112	16	0.3530318380419004
1112	17	0.7202328323098115
1112	18	0.3549462919608004
1112	19	0.4188582186708558
1112	20	0.22000559753320204
1112	21	0.2689809687172229
1112	22	0.11440932802196357
1112	23	0.28834035013152004
1112	24	0.274539288109537
1112	25	0.36475070582572044
1113	1	-0.03429355144189472
1113	2	0.11016716942623361
1113	3	-0.03012548439023277
1113	4	0.12068761296174557
1113	5	0.09263783689946337
1113	6	-0.3274992211301466
1113	7	-0.13218625447193233
1113	8	-0.010077621535359071
1113	9	-0.1403863406588428
1113	10	-0.06639490149195382
1113	11	-0.03857215853030027
1113	12	-0.28550891918697374
1113	13	0.03731251576862604
1113	14	0.2894542991127764
1113	15	-0.07318525885590788
1113	16	-0.08816863940197806
1113	17	-0.07598955297019927
1113	18	-0.2542724934963587
1113	19	-0.6679091583968386
1113	20	-0.3006848866784608
1113	21	-0.16590982199421234
1113	22	-0.6499004926666672
1113	23	-3.9997849329009263
1113	24	-1.6955574902439423
1113	25	-0.8510557254712481
1114	1	-0.6141868338283594
1114	2	-0.8204682330418755
1114	3	-0.8169485090750257
1114	4	-0.6603208462709738
1114	5	-0.5571264968004215
1114	6	-1.3321199837781315
1114	7	-1.1884605424349388
1114	8	-0.9263717868175405
1114	9	-1.253035281707625
1114	10	-0.8469156840807344
1114	11	-1.0398224249805281
1114	12	-2.206834299476906
1114	13	-1.4239309433080578
1114	14	-3.177784885123735
1114	15	-1.9558405788421354
1114	16	-1.9894954425228946
1114	17	-1.7856877329630834
1114	18	-1.7925744161165653
1114	19	-1.886625124834957
1114	20	-1.4046479470239461
1114	21	-1.2276011819191481
1114	22	-1.2958360093315093
1114	23	-5.657472160797724
1114	24	-2.9777244459727434
1114	25	-3.0236514716826264
1115	1	-1.4603145418637418
1115	2	-1.506617525906961
1115	3	-1.0911576832897616
1115	4	-1.1766906222124973
1115	5	-1.0898443314314932
1115	6	-1.2758097978572671
1115	7	-1.6666625128782897
1115	8	-2.0059620723199494
1115	9	-3.419953285839199
1115	10	-3.9414157900185494
1115	11	-2.8373922115158337
1115	12	-3.0862440934278546
1115	13	-2.2176671051810395
1115	14	-2.8535816699110654
1115	15	-2.6239932882017385
1115	16	-2.7298025297626864
1115	17	-2.5897586583298917
1115	18	-5.132207076744777
1115	19	-3.1391558468011596
1115	20	-3.4778863335910337
1115	21	-3.1280577738996556
1115	22	-2.6348816420451087
1115	23	-3.1518080631230596
1115	24	-2.8188619532559684
1115	25	-2.9592531984690034
1116	1	-2.069029045182205
1116	2	-1.7229908802753648
1116	3	-2.2274263156475023
1116	4	-1.943222555625617
1116	5	-2.048366799062869
1116	6	-4.493720797748466
1116	7	-3.004136672382149
1116	8	-3.2771006861813383
1116	9	-4.292915422712227
1116	10	-3.7855886221369275
1116	11	-3.212021740402786
1116	12	-2.971001192764163
1116	13	-4.34519348968762
1116	14	-7.363166317936308
1116	15	-5.442446666506601
1116	16	-4.268893216303866
1116	17	-5.235216715124583
1116	18	-3.9932858494572123
1116	19	-3.630718516804425
1116	20	-4.423038384510413
1116	21	-5.149396039594357
1116	22	-4.907193414431138
1116	23	-3.936792558825374
1116	24	-4.859204135825873
1116	25	-4.827219143415146
1201	1	0.5342289043807362
1201	2	0.6794097200429654
1201	3	1.0617068233376301
1201	4	1.0137291126917773
1201	5	0.45902164794995315
1201	6	0.9545583906552508
1201	7	0.9599575165767931
1201	8	1.135179312068857
1201	9	1.5295975241755286
1201	10	1.2807012939284306
1201	11	1.4635208959952166
1201	12	1.3038553174705214
1201	13	1.243960288044292
1201	14	1.4205236320035155
1201	15	1.3499463288822113
1201	16	1.3316959974832656
1201	17	1.6897774241443315
1201	18	1.4936029379827644
1201	19	1.4838596171163658
1201	20	1.508369189236653
1201	21	1.7592323586482053
1201	22	1.537935000075656
1201	23	1.8648598308386468
1201	24	1.6370806093360812
1201	25	1.735113517289463
1202	1	1.2205153925675667
1202	2	1.1871553844136535
1202	3	1.2170794482090237
1202	4	1.3551125168241072
1202	5	1.1888473924571983
1202	6	1.4158170285147733
1202	7	1.3457916664804799
1202	8	1.4337578409435139
1202	9	1.7137756108513855
1202	10	1.5978475947715793
1202	11	1.5629134315220128
1202	12	1.7114946901954582
1202	13	1.6455625401284735
1202	14	2.167704534849122
1202	15	1.7230099127895357
1202	16	1.5215828024692328
1202	17	1.8709313618053756
1202	18	1.9116090727724924
1202	19	1.9642595334456274
1202	20	1.8332083279815805
1202	21	1.8756037470874887
1202	22	1.8448549215358696
1202	23	1.9206526989193833
1202	24	2.0033635101133314
1202	25	2.27405647162238
1203	1	1.1088407807253162
1203	2	1.2346820881336171
1203	3	1.1216762498453754
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2211	17	0.6187664146609393
2211	18	0.1437866229939928
2211	19	0.3250667368181581
2211	20	0.14333978432117078
2211	21	0.2879971356941553
2211	22	0.24027445589066332
2211	23	0.3119781121573837
2211	24	0.07884306134219798
2211	25	0.5004776318337179
2212	1	0.1878703948644187
2212	2	0.2940857062930675
2212	3	-0.06149945286845693
2212	4	0.33730211041950753
2212	5	0.05053987347779554
2212	6	-0.07750924391103808
2212	7	0.13121953214890425
2212	8	0.3183940287117082
2212	9	0.4437929661301041
2212	10	0.056907252111855655
2212	11	-0.17767985699024536
2212	12	0.3304115197245565
2212	13	0.4728870951543769
2212	14	0.380036386703253
2212	15	0.0972022700642583
2212	16	0.03865671199164922
2212	17	0.30971610916515147
2212	18	0.4872356736744976
2212	19	0.17435650264235392
2212	20	0.2558028720805652
2212	21	0.3869272339775591
2212	22	0.444161276082653
2212	23	0.5378043015666805
2212	24	0.36979002621244916
2212	25	0.4924759777265564
2213	1	-0.48196121775691836
2213	2	-0.2303379882576735
2213	3	-0.4041955119842129
2213	4	-0.273661507444956
2213	5	-0.39176754635793287
2213	6	-0.2076197317339421
2213	7	-0.3369826215460847
2213	8	-0.11826741857039735
2213	9	-0.6630259702412715
2213	10	-0.3300291223267138
2213	11	-0.4857227256375225
2213	12	-0.4519956696234573
2213	13	-0.5853130634567094
2213	14	-0.5994506278279204
2213	15	-0.48531857396728384
2213	16	-0.44552181317034467
2213	17	-0.4445228697195063
2213	18	-0.4332899103087726
2213	19	-0.41219892948095094
2213	20	-0.3034467159786516
2213	21	-0.5039294228821802
2213	22	-0.7027442951795564
2213	23	-0.35099600202305226
2213	24	-0.15225756553748226
2213	25	-0.06169437946238787
2214	1	-2.5822060713868495
2214	2	-1.3480359758780445
2214	3	-1.6074574361684633
2214	4	-3.5550888029790997
2214	5	-1.7585451043427902
2214	6	-1.4103531492427237
2214	7	-1.2076754473122762
2214	8	-1.0696518036412606
2214	9	-0.9165648321875892
2214	10	-1.0342904153931727
2214	11	-1.0687124702045097
2214	12	-0.996779479094819
2214	13	-1.4048144315388882
2214	14	-1.2407777632913692
2214	15	-1.6960078525847209
2214	16	-1.2986963990512947
2214	17	-3.364281241010371
2214	18	-3.099217697702766
2214	19	-2.068138574599679
2214	20	-1.8139598653224596
2214	21	-1.4645150008831962
2214	22	-2.807556730114932
2214	23	-1.7614553894809077
2214	24	-2.721594220988237
2214	25	-2.624602323028654
2215	1	-2.006252647298634
2215	2	-3.45050327078307
2215	3	-2.1949881105356077
2215	4	-3.746132347510752
2215	5	-2.4632062157998007
2215	6	-2.1653417648196402
2215	7	-2.4142229289076553
2215	8	-1.6868221722485721
2215	9	-4.207800231898137
2215	10	-2.535356087765056
2215	11	-2.1702095868599756
2215	12	-2.099045408814824
2215	13	-2.1793082798521937
2215	14	-1.821091866709267
2215	15	-2.0204126825119246
2215	16	-2.112896493258148
2215	17	-2.621591532807308
2215	18	-2.218622392335682
2215	19	-2.2145706015681625
2215	20	-2.016631362651907
2215	21	-2.7030287130247714
2215	22	-2.6552443931412633
2215	23	-2.46385787448229
2215	24	-2.2189787065954185
2215	25	-3.1363792308948213
2216	1	-3.1326757625951274
2216	2	-3.3001225245915187
2216	3	-4.380606512545221
2216	4	-4.845763234682899
2216	5	-4.876474165685636
2216	6	-3.8835761751264073
2216	7	-5.620352331347114
2216	8	-3.3795440244378163
2216	9	-4.232751233747646
2216	10	-3.2107047929125265
2216	11	-3.445105971052797
2216	12	-3.756614390718042
2216	13	-3.2957373498534928
2216	14	-4.266859096903961
2216	15	-3.8950416965153423
2216	16	-4.2205043187655775
2216	17	-3.7868791529365744
2216	18	-3.366915048331201
2216	19	-4.016774236698556
2216	20	-4.600003539589455
2216	21	-4.439323230358482
2216	22	-4.02976259442606
2216	23	-4.140776097850761
2216	24	-4.647810914055757
2216	25	-4.675071404204335
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	90770.0
3	2.0
4	801.0
5	4887.0
6	6085.0
7	5649.0
8	4904.0
9	4560.0
10	3978.0
11	3900.0
12	3701.0
13	3652.0
14	3428.0
15	3479.0
16	3727.0
17	3882.0
18	4469.0
19	5362.0
20	6805.0
21	8895.0
22	11145.0
23	13545.0
24	15507.0
25	17705.0
26	19785.0
27	21704.0
28	24544.0
29	28567.0
30	34731.0
31	42757.0
32	53972.0
33	68200.0
34	88446.0
35	114700.0
36	155797.0
37	237718.0
38	654752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83479429772044	18.153310891715428	12.553543591713169	24.45835121885096
2	15.337768685393623	19.950696209158863	40.97196810960101	23.739566995846502
3	19.289710450554114	25.445718345412438	28.728581030947797	26.53599017308565
4	12.648734462259053	16.302693208430913	37.281908665105384	33.76666366420465
5	13.479753712912396	38.53992088052644	34.194260995180436	13.78606441138073
6	32.20430265022883	35.57933898637366	18.31071290952807	13.905645453869434
7	29.043368726564754	31.479507679550046	21.435138448226134	18.04198514565907
8	26.221350710967233	35.48187153285494	20.395448231949715	17.901329524228107
9	28.004682761499723	14.658530800480671	19.611260937194547	37.725525500825064
10	16.78337753303146	28.12447356159421	32.64681539625635	22.445333509117983
11	34.73096095514101	22.36938473811595	23.98100489919909	18.918649407543956
12	24.523578511083542	24.451063052951497	30.30937675020312	20.715981685761843
13	29.44691921954726	21.825769948637227	25.41445988526333	23.31285094655218
14	22.108210584434058	22.039645852171734	26.856980278484365	28.995163284909843
15	23.92641873289101	30.091035747826638	23.175552684847524	22.806992834434826
16	22.89874031744744	27.525407457361272	26.88691815246559	22.688934072725694
17	22.277866490568336	28.030568536922612	26.939326085805114	22.75223888670394
18	22.16676433465561	26.674348629642985	29.100872250270466	22.05801478543094
19	24.019503718924682	26.352332406114126	27.263005531199695	22.3651583437615
20	24.76760073358105	26.183378360485786	27.378067504105967	21.67095340182719
21	24.322746926186216	25.98538089939862	26.55549765502866	23.136374519386504
22	24.44178486847777	27.118941083067234	26.426606127505252	22.01266792094974
23	23.32027301585155	26.884364918116653	27.281921433006428	22.51344063302536
24	23.927518251711927	26.04335337951053	27.514364173345907	22.514764195431635
25	23.353893583241405	26.57883676461143	27.544556285092618	22.522713367054546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	289.0
1	289.0
2	579.0
3	869.0
4	869.0
5	869.0
6	1577.5
7	2286.0
8	2286.0
9	2286.0
10	2543.0
11	2800.0
12	2800.0
13	2800.0
14	3918.0
15	5036.0
16	5036.0
17	5036.0
18	10433.0
19	15830.0
20	15830.0
21	15830.0
22	25915.0
23	36000.0
24	36000.0
25	36000.0
26	55275.5
27	74551.0
28	74551.0
29	74551.0
30	96486.5
31	118422.0
32	118422.0
33	118422.0
34	136405.0
35	154388.0
36	154388.0
37	154388.0
38	166991.5
39	179595.0
40	179595.0
41	179595.0
42	193894.5
43	208194.0
44	208194.0
45	208194.0
46	224485.5
47	240777.0
48	240777.0
49	240777.0
50	235062.0
51	229347.0
52	229347.0
53	229347.0
54	206825.0
55	184303.0
56	184303.0
57	184303.0
58	160597.0
59	136891.0
60	136891.0
61	136891.0
62	114779.0
63	92667.0
64	92667.0
65	92667.0
66	72121.0
67	51575.0
68	51575.0
69	51575.0
70	38580.5
71	25586.0
72	25586.0
73	25586.0
74	18275.0
75	10964.0
76	10964.0
77	10964.0
78	7598.5
79	4233.0
80	4233.0
81	4233.0
82	2826.5
83	1420.0
84	1420.0
85	1420.0
86	903.0
87	386.0
88	386.0
89	386.0
90	225.5
91	65.0
92	65.0
93	65.0
94	45.5
95	26.0
96	26.0
97	26.0
98	18.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.337234050337994
2	0.009963349509234675
3	0.009287868186574696
4	0.010751411052337982
5	0.011820923146549613
6	0.07987566640454238
7	0.07920018508188241
8	0.10222284016254332
9	0.08387226423028059
10	0.0907396576773237
11	0.08595499830848219
12	0.0966501192505985
13	0.08387226423028059
14	0.08702451040269382
15	0.08409742467116725
16	0.08668676974136383
17	0.09822624233680512
18	0.10081558740700171
19	0.09400448407018025
20	0.09321642252707694
21	0.08083259827831069
22	0.10087187751722337
23	0.09394819395995858
24	0.0905707873466587
25	0.08809402249690546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1776511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.6150856980933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.40817935948063	42.69240536774186
2	13.88625297719383	15.723428046584642
3	4.788966329421334	8.133832174364166
4	2.1778384982177	4.931940528528076
5	1.1431404150411697	3.2359496281254896
6	0.6538608373311359	2.2211032404077584
7	0.43258872481868405	1.7143733409347093
8	0.29266351112914735	1.3255335810625248
9	0.20817335155671807	1.060717692459861
>10	0.9132521334439531	8.742091785194518
>50	0.052382359883587234	2.063095188735834
>100	0.03598631866460542	3.964235729632043
>500	0.004710651334708529	1.8553385950609316
>1k	0.0019043058587119585	2.009623870161726
>5k	1.0022662414273465E-4	0.32633123100576256
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5751	0.32372442388479444	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3300	0.18575736373149393	No Hit
GTCCTACAGTGGACATTTCTAAATT	3297	0.18558849340082892	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3216	0.18102899447287407	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2966	0.16695646691745786	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2740	0.15423490200736162	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2607	0.14674831734788019	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2243	0.1262587172271942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.629011022166483E-5
13	0.0	0.0	0.0	0.0	5.629011022166483E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1470	0.0	12.956914	1
TACCGTC	60	4.0945318E-4	12.665935	7
CCGCCCC	55	0.0030726616	12.088848	9
TAGGACC	485	0.0	11.94709	4
AGGACCG	195	0.0	11.690972	5
TATACCG	65	8.0320984E-4	11.690972	5
ACGAAAT	130	2.6211637E-9	11.690972	12
CGAAATC	130	2.6229827E-9	11.690313	13
GACTAGT	60	0.0058824453	11.081756	11
CCGTTGG	60	0.005883679	11.081444	13
GTATCAA	3275	0.0	11.07905	1
CCTATAC	130	3.2718162E-8	10.960286	3
TAAGGCG	70	0.001493004	10.855904	5
CTTACGG	70	0.001493004	10.855904	3
CCTAATA	135	5.696711E-8	10.55435	2
GGACCGT	100	2.4004301E-5	10.449396	6
CCTTATA	165	9.313226E-10	10.3624525	2
GATATAC	305	0.0	10.303932	1
ATTTCTA	1155	0.0	10.197395	15
GTCCTAC	1495	0.0	10.192194	1
>>END_MODULE