FastQCFastQC Report
Thu 26 May 2016
SRR1512909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1776511
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73070.41131183538970484No Hit
TATCAACGCAGAGTACTTTTTTTTT53480.3010395094654635No Hit
GGTATCAACGCAGAGTACTTTTTTT43380.24418649814158203No Hit
GTCCTACAGTGGACATTTCTAAATT30800.17337353948272766No Hit
GTCCTAAAGTGTGTATTTCTCATTT27250.15339055035403665No Hit
CTGTAGGACGTGGAATATGGCAAGA26560.14950653274874176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23300.13115595681647904No Hit
CTTTAGGACGTGAAATATGGCGAGG22910.12896064251783412No Hit
GTACTGGTTCACTATCGGTCAGTCA21990.12378195237744095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT456.7995745E-414.76726915
TCGTCCG400.00529160514.24507910
GGTCGGT551.9717941E-413.808743511
CGACCAT500.001503602513.29540710
GGTATCA16650.012.8547611
TCGAACT1207.4942363E-1012.65730219
CCGATCG550.003072799212.0887769
CCGACCA550.003072799212.0887769
TAGGACC4450.011.7527424
CGCGCCT1850.011.28926412
GCGCATT600.00590617711.07576311
GTCCTAA8100.011.0392251
TTAGGAC10200.011.00223
GGCGAGG7300.010.92342619
AGGACGT16400.010.842625
GGACGTG16350.010.7591566
TCGCGCG1803.6379788E-1110.5536949
ATCGCGC1909.094947E-1210.5005148
GTCCTAC15650.010.45465851
TAGGACG17450.010.2991864
Produced by FastQC (version 0.11.5)