##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512909_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1776511
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.134647069452427	31.0	30.0	34.0	26.0	34.0
2	30.154865914142945	31.0	30.0	34.0	26.0	34.0
3	30.352415493064775	31.0	30.0	34.0	26.0	34.0
4	33.95555501767228	37.0	35.0	37.0	30.0	37.0
5	33.51543052646451	37.0	35.0	37.0	28.0	37.0
6	33.37113251761458	37.0	35.0	37.0	27.0	37.0
7	33.2148345830676	37.0	35.0	37.0	26.0	37.0
8	33.16307638961988	37.0	35.0	37.0	26.0	37.0
9	34.472963578609985	39.0	35.0	39.0	26.0	39.0
10	34.40127249423167	39.0	35.0	39.0	26.0	39.0
11	34.377961633786676	39.0	35.0	39.0	26.0	39.0
12	34.235402989342596	39.0	35.0	39.0	25.0	39.0
13	34.21652722668196	39.0	35.0	39.0	25.0	39.0
14	35.2871583682848	39.0	35.0	41.0	25.0	41.0
15	35.27925861421629	40.0	35.0	41.0	25.0	41.0
16	35.27290402367337	40.0	35.0	41.0	25.0	41.0
17	35.261128132615	40.0	35.0	41.0	25.0	41.0
18	35.182344494348754	40.0	35.0	41.0	25.0	41.0
19	35.196643871048366	40.0	35.0	41.0	25.0	41.0
20	35.163651111645244	40.0	35.0	41.0	25.0	41.0
21	35.134625116309444	40.0	35.0	41.0	25.0	41.0
22	35.09089558128264	40.0	35.0	41.0	24.0	41.0
23	34.926452467786575	40.0	35.0	41.0	23.0	41.0
24	34.879319632695776	40.0	35.0	41.0	23.0	41.0
25	34.81613792427967	40.0	35.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6225808436441937
1101	2	0.5665746727207086
1101	3	0.5649382825544862
1101	4	0.6645022894821864
1101	5	0.6079935437387647
1101	6	0.7172333870114755
1101	7	0.7576631101785551
1101	8	0.5551564720025297
1101	9	0.8994464601466987
1101	10	0.6263882241831666
1101	11	0.959008263556477
1101	12	1.0379033334702257
1101	13	1.0563277409648606
1101	14	1.2467296368984648
1101	15	0.8356955074987695
1101	16	1.0738464254006388
1101	17	1.1805330601511699
1101	18	1.0506215703049762
1101	19	0.8987933551843028
1101	20	1.098012949849327
1101	21	1.1509013488982234
1101	22	1.0189058005444878
1101	23	1.201905367884919
1101	24	1.2790511411834231
1101	25	1.301927407386124
1102	1	0.7121208598431039
1102	2	0.8777994842317192
1102	3	0.6036993377666775
1102	4	0.6163540205367966
1102	5	0.9083047476221537
1102	6	0.773781986458097
1102	7	0.9077261663365874
1102	8	0.9480675465120711
1102	9	1.1055697023203948
1102	10	0.8868990334219831
1102	11	1.0115063202309216
1102	12	0.9776474668013861
1102	13	1.100360780217322
1102	14	1.1020837026616874
1102	15	1.2231940805814006
1102	16	1.287026793191984
1102	17	1.2709121037632016
1102	18	0.7165326502504001
1102	19	1.0413467490732913
1102	20	1.2169662176259948
1102	21	1.1480211501626059
1102	22	1.0765896826294679
1102	23	1.2748181275159283
1102	24	1.0616786260580966
1102	25	1.2010954466113972
1103	1	0.817244037181343
1103	2	1.0518721206782047
1103	3	0.7307867495869758
1103	4	0.6051664846742426
1103	5	0.8187659612111062
1103	6	0.6307995506731743
1103	7	0.7766896540336177
1103	8	0.8907718365178141
1103	9	1.0471285000386885
1103	10	0.8247782272316826
1103	11	0.9600651727978331
1103	12	1.2409973039431819
1103	13	1.1563865337529435
1103	14	1.1847331862408552
1103	15	1.3005480355886547
1103	16	1.177335886248997
1103	17	1.1455434577646173
1103	18	1.1730888532071617
1103	19	1.1255625826035214
1103	20	1.083742089297111
1103	21	1.2373800963940838
1103	22	1.1280107892214275
1103	23	1.1180834555950483
1103	24	1.0768284471672231
1103	25	1.0942339900008733
1104	1	0.8142216979816759
1104	2	1.0229427261674147
1104	3	0.8174699153108378
1104	4	0.6994147300126059
1104	5	0.7859447533753681
1104	6	0.6972293380186585
1104	7	0.6249006848318572
1104	8	0.7060745811228841
1104	9	0.7259997550145982
1104	10	0.7856533319876036
1104	11	0.8735562947337243
1104	12	0.7385450988310893
1104	13	0.6291468522109369
1104	14	0.8711308920862351
1104	15	0.9882676301832518
1104	16	0.9284248240239776
1104	17	0.9658919021392265
1104	18	0.5664956466284892
1104	19	1.019862289286948
1104	20	0.8657971385323933
1104	21	0.8563634402030118
1104	22	0.8782377167303324
1104	23	0.9125967334077458
1104	24	1.1097728741523483
1104	25	0.9875433604178028
1105	1	0.49902186792654035
1105	2	0.544996741597636
1105	3	0.6268311736753134
1105	4	0.3870657250590952
1105	5	0.3141337784132645
1105	6	0.42954918197837344
1105	7	0.4321399498203178
1105	8	0.35590473281798296
1105	9	0.6145638174693389
1105	10	0.3462709270746487
1105	11	0.6022833497630273
1105	12	0.64719316812225
1105	13	0.7553390250912173
1105	14	0.8499928408517263
1105	15	0.8594250898937617
1105	16	0.7497262540713905
1105	17	0.45263987197401434
1105	18	0.6572226437220436
1105	19	0.8733613188631466
1105	20	0.7997824421639947
1105	21	0.6950575684148248
1105	22	0.5923124345349535
1105	23	0.8206696938279734
1105	24	0.6745073835813926
1105	25	0.5647572302361752
1106	1	0.10907124720326777
1106	2	0.39221803715368253
1106	3	-1.3855888842969222
1106	4	-0.2860934574613623
1106	5	0.07888204450705416
1106	6	0.09637126844562971
1106	7	0.3401513268098384
1106	8	0.39536458463203417
1106	9	0.5660291400603654
1106	10	0.3762867174662716
1106	11	0.4626262383577284
1106	12	0.6589951885276335
1106	13	0.6732262574261298
1106	14	0.8040369616067977
1106	15	0.7085328826945911
1106	16	0.7070464161293728
1106	17	0.7457819049924623
1106	18	0.9224831448657156
1106	19	0.8093534622946805
1106	20	0.8012677092122829
1106	21	0.8833908607797554
1106	22	0.6540012195852114
1106	23	0.7977518411911717
1106	24	0.7836690491770284
1106	25	0.8697572087765408
1107	1	0.601959638294634
1107	2	0.642168944709752
1107	3	0.6239452112627575
1107	4	0.445444290626952
1107	5	0.5015690028865691
1107	6	0.516946934191246
1107	7	0.3753965261022998
1107	8	0.4983604049788397
1107	9	0.5341566488239664
1107	10	0.501100921936235
1107	11	0.5741602063913689
1107	12	0.7702798202936592
1107	13	0.5670289492416742
1107	14	0.5850170881217736
1107	15	0.5178042288894247
1107	16	0.6852849360550621
1107	17	0.6625377594410509
1107	18	0.7644484074842524
1107	19	0.774091242594757
1107	20	0.7725845120887769
1107	21	0.7784520466042508
1107	22	0.6292591359164419
1107	23	0.8064580336472744
1107	24	0.8116652061214396
1107	25	0.7134035541775106
1108	1	0.41302930139660887
1108	2	0.3775184537274363
1108	3	0.3576123614218716
1108	4	0.2113717131737758
1108	5	0.461775138734545
1108	6	0.39473794610318436
1108	7	0.2736738559086902
1108	8	0.3069575583409403
1108	9	0.43885858242840214
1108	10	0.41065592620485347
1108	11	0.41462133883541696
1108	12	0.5273399669976868
1108	13	0.536579520481304
1108	14	0.5797276563898066
1108	15	0.5840395277352499
1108	16	0.5065522708425263
1108	17	0.6125859260060267
1108	18	0.6056032358931418
1108	19	0.6773803308887594
1108	20	0.7645207836434693
1108	21	0.7243017449999556
1108	22	0.7052383299089584
1108	23	0.7368937005319367
1108	24	0.6422666244471458
1108	25	0.6521440867986357
1109	1	0.45461805621777884
1109	2	0.4658962055910685
1109	3	0.6026111878929861
1109	4	0.5589664101784706
1109	5	0.32214405186199
1109	6	0.5507454591521466
1109	7	0.6167061436616734
1109	8	0.7136155636868082
1109	9	0.8387963638366216
1109	10	0.4534051501665246
1109	11	0.7038452635472225
1109	12	0.8967364281766734
1109	13	0.8032304168910471
1109	14	0.9958574258914865
1109	15	0.9400479032201403
1109	16	0.9135659694051483
1109	17	0.8977315144306601
1109	18	0.9998819591676451
1109	19	0.8702673289895486
1109	20	1.0417743441177834
1109	21	1.0535525530716114
1109	22	1.0205238362420062
1109	23	0.9660203794328694
1109	24	1.0383546150884868
1109	25	1.025519211312826
1110	1	0.3160945743182211
1110	2	0.13848996891188747
1110	3	0.2883212510756685
1110	4	0.25784236179663367
1110	5	0.4400555423650445
1110	6	0.3692272182518366
1110	7	0.21672898371897986
1110	8	0.31353678381000094
1110	9	0.23578123199330747
1110	10	0.319093277123045
1110	11	0.3457493182070266
1110	12	0.58569307725935
1110	13	0.4603516902669895
1110	14	0.40463692406977714
1110	15	0.36301559614229006
1110	16	0.4311873789664631
1110	17	0.4613917618605399
1110	18	0.37108992949266906
1110	19	0.4833792318350305
1110	20	0.48312498541041293
1110	21	0.6207962467119401
1110	22	0.5575188663921082
1110	23	0.5764384990488196
1110	24	0.5455221182060868
1110	25	0.5394246534755709
1111	1	-0.0022923993029984047
1111	2	-0.0994983839444501
1111	3	-0.1947867546691242
1111	4	0.09104944704136386
1111	5	0.21186545516296462
1111	6	0.19701129160096542
1111	7	0.0952599407602932
1111	8	0.13931987545399238
1111	9	0.14805557615973441
1111	10	0.1184830443619731
1111	11	0.24075655979738997
1111	12	0.34777562532956807
1111	13	0.35735688360836093
1111	14	0.10456299318909146
1111	15	0.2293297781455692
1111	16	0.10708530587184839
1111	17	0.1419661199647564
1111	18	-0.10105267226834513
1111	19	-0.2440679106152288
1111	20	0.015972112104911673
1111	21	0.14463531978837807
1111	22	-0.23922272094026198
1111	23	-0.1071391495855849
1111	24	0.11163147531042483
1111	25	0.016873916893722196
1112	1	0.01911977054618319
1112	2	-0.37874341687822266
1112	3	-0.09557944799781026
1112	4	0.025537068188199896
1112	5	0.03481600725012157
1112	6	-0.06453344789269266
1112	7	-0.015712584358844595
1112	8	-0.14614900897036165
1112	9	-0.0693288181275733
1112	10	-0.3680297554446099
1112	11	-0.13885530397079293
1112	12	0.19590275080682318
1112	13	-0.10776129630197318
1112	14	-0.39600977345666877
1112	15	-0.5108679595059513
1112	16	-0.6030223624757696
1112	17	-0.424478370025021
1112	18	-0.21035782913178735
1112	19	-0.2577662011516324
1112	20	-0.2250437233526199
1112	21	-0.24561500635847722
1112	22	-0.15064111304548078
1112	23	0.07989118974214193
1112	24	-0.05831490528792216
1112	25	-0.0732469323276419
1113	1	-0.8041372744001194
1113	2	-0.5931615521479898
1113	3	-0.7022688105762462
1113	4	-2.0707154572359876
1113	5	-0.8677548520788747
1113	6	-0.4973121792475368
1113	7	-0.6574129983234798
1113	8	-0.5622272014014413
1113	9	-0.5092797041878256
1113	10	-0.5387509296206545
1113	11	-0.7892096381035358
1113	12	-0.3917765780526423
1113	13	-0.41540044829927325
1113	14	-0.5678223407471634
1113	15	-0.7473968645539983
1113	16	-0.9164566194737276
1113	17	-0.9825921168298137
1113	18	-0.847416011106283
1113	19	-0.6751809565266242
1113	20	-0.40229426242634503
1113	21	-0.550620019719112
1113	22	-0.5497051191155009
1113	23	-0.3243238819294163
1113	24	-0.2479888818975695
1113	25	-0.5752980116247102
1114	1	-0.9665369026656911
1114	2	-2.287678284164386
1114	3	-1.5341455934125676
1114	4	-0.9636884176289229
1114	5	-0.8668374170041346
1114	6	-0.532648504228483
1114	7	-0.43424050307462636
1114	8	-0.5514680337945919
1114	9	-0.991202742254643
1114	10	-1.0873715505144759
1114	11	-1.174199670352607
1114	12	-1.1124909873106645
1114	13	-1.2354021461756588
1114	14	-1.1180097947630117
1114	15	-1.3761007871773785
1114	16	-1.7524887035191412
1114	17	-1.6963530346774576
1114	18	-1.3973793295712937
1114	19	-1.8647566566890603
1114	20	-1.1656195506818534
1114	21	-1.4769294077060806
1114	22	-1.06892920547552
1114	23	-1.0259508018917387
1114	24	-0.9882058609787805
1114	25	-1.4953154717180013
1115	1	-0.8194108631508854
1115	2	-0.9166743909497015
1115	3	-0.47351142317049977
1115	4	-0.215907020309146
1115	5	-0.6636530587224456
1115	6	-0.42394505065573185
1115	7	-0.37665336922219694
1115	8	-0.6771268345785302
1115	9	-1.6336688421540018
1115	10	-1.0411291647948957
1115	11	-1.5269842458068155
1115	12	-2.185254772468781
1115	13	-2.2442862237221384
1115	14	-1.6457609998056526
1115	15	-1.8695412501256286
1115	16	-1.5902720871729343
1115	17	-1.4397451634978395
1115	18	-1.945920698287857
1115	19	-2.3680752911506318
1115	20	-2.556262063526809
1115	21	-1.8680767142371408
1115	22	-1.747917366924753
1115	23	-1.811370448059435
1115	24	-2.180326868447395
1115	25	-2.2807224053479587
1116	1	-1.5707363446001281
1116	2	-1.8215315882078897
1116	3	-1.9086014568991452
1116	4	-1.4594697447134592
1116	5	-2.0042424940396053
1116	6	-2.759481172949279
1116	7	-2.1156575581026225
1116	8	-2.604685043696147
1116	9	-3.730866372346579
1116	10	-2.2665986458089336
1116	11	-3.2361693181602043
1116	12	-5.299576663449088
1116	13	-4.477155062803849
1116	14	-3.4884837720828834
1116	15	-4.719670452606319
1116	16	-4.17784552858301
1116	17	-3.9672720920148663
1116	18	-3.9655363549084655
1116	19	-4.392482591770804
1116	20	-6.077613385472194
1116	21	-5.129296385654836
1116	22	-4.1448102512681295
1116	23	-4.895916471343085
1116	24	-4.896432569676858
1116	25	-4.254993159346437
1201	1	0.569259976318925
1201	2	0.8017608078720926
1201	3	0.8557043352978368
1201	4	0.624129798542107
1201	5	0.17225636690347557
1201	6	0.6850939773265949
1201	7	0.8778569400561551
1201	8	0.7504320755834684
1201	9	0.6761507454748639
1201	10	0.8642316599473716
1201	11	0.5907918955775742
1201	12	0.9733718843795245
1201	13	0.918780786587142
1201	14	0.8651594714756428
1201	15	0.867075097444328
1201	16	0.7827212854338441
1201	17	0.6136421708173287
1201	18	0.9486485211524993
1201	19	1.1542406145931068
1201	20	1.1870221780048524
1201	21	0.818918867500706
1201	22	0.8119022207041127
1201	23	0.8273445740318692
1201	24	0.5042128959119552
1201	25	0.5781840747698581
1202	1	1.0059147860824247
1202	2	1.0815827467445516
1202	3	1.0434183357225777
1202	4	1.0084455533910983
1202	5	1.0953772216224351
1202	6	1.2008116481929463
1202	7	1.2470591352953306
1202	8	1.0388206846532313
1202	9	1.520110005489144
1202	10	1.5418069702662862
1202	11	1.3400414137522745
1202	12	1.4030064955367934
1202	13	1.5198573820396462
1202	14	1.6290113167827087
1202	15	1.5520105852814652
1202	16	1.6578763717046527
1202	17	1.5902254080339873
1202	18	1.546866100204582
1202	19	1.66653813752405
1202	20	1.661939185892365
1202	21	1.7148738769136642
1202	22	1.588550313389156
1202	23	1.7743367386822655
1202	24	1.603726386754218
1202	25	1.6767830156856505
1203	1	0.8548812403279662
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2211	8	-0.2558256769695575
2211	9	-0.036739707287431145
2211	10	-0.11977332808947239
2211	11	-0.17523810023492814
2211	12	-0.034935789730468514
2211	13	0.13773664673834674
2211	14	-0.13024827122910665
2211	15	0.01326428693322157
2211	16	-0.06384364379844953
2211	17	-0.22510089775999376
2211	18	-0.1541744246618748
2211	19	-0.06381147411431698
2211	20	-0.07248498008676307
2211	21	-0.026075484917882363
2211	22	-0.054358615708238744
2211	23	-0.24059815384236316
2211	24	0.1948942809661176
2211	25	-0.11490221890120722
2212	1	-0.17096737583066357
2212	2	-0.05875639023821222
2212	3	0.0208150747712601
2212	4	-0.14010760982990433
2212	5	-0.37161425707585494
2212	6	-0.022391841325038797
2212	7	-0.24251516217844227
2212	8	0.17061100655578798
2212	9	-0.052678218583331216
2212	10	-0.16623106040906777
2212	11	0.04611429563750846
2212	12	0.1568869412070839
2212	13	0.18030937011838688
2212	14	0.2323019671100326
2212	15	-0.04675385875499671
2212	16	-0.08589959644772449
2212	17	-0.1685402033921335
2212	18	-0.23840748489150343
2212	19	-0.08973725689744327
2212	20	-0.2105681061146072
2212	21	-0.08377981938528478
2212	22	0.020373149922328082
2212	23	-0.38656928426414083
2212	24	-0.36122042437563806
2212	25	-0.22290401331665777
2213	1	-0.5622125187969971
2213	2	-0.22244134466337329
2213	3	-0.3191640706665275
2213	4	-0.47411682091399854
2213	5	-0.36486237237255637
2213	6	-0.3568138547974087
2213	7	-0.8529495658493929
2213	8	-0.7865923376796644
2213	9	-0.4946513374125985
2213	10	-0.4934696230449376
2213	11	-0.37784179873110446
2213	12	-0.5292507486848876
2213	13	-0.5426845270693121
2213	14	-0.5696953378035019
2213	15	-0.8570194653083689
2213	16	-1.4702435106185234
2213	17	-0.9498943254231023
2213	18	-0.6740955150390988
2213	19	-0.5987340896538882
2213	20	-0.609942928768497
2213	21	-0.5915187153648276
2213	22	-0.6883760014011457
2213	23	-0.8508233928541458
2213	24	-0.6156142998892946
2213	25	-0.7975980781143761
2214	1	-1.2789851046988119
2214	2	-0.3848184836990569
2214	3	-0.5440704693874387
2214	4	-0.7183658772416877
2214	5	-0.9972913940713113
2214	6	-1.7006400661665353
2214	7	-1.0849943703263563
2214	8	-0.9028189056653417
2214	9	-0.6823744786227905
2214	10	-0.9577183650683097
2214	11	-1.037083620116718
2214	12	-0.8397235931218106
2214	13	-1.0683793674205475
2214	14	-0.7962781142701374
2214	15	-1.0045161436531558
2214	16	-0.9276314383480724
2214	17	-0.9188713005187097
2214	18	-0.8401232063061528
2214	19	-0.928314919942892
2214	20	-1.144708354078368
2214	21	-1.478799149696421
2214	22	-1.7706752669194898
2214	23	-1.8195892029887162
2214	24	-1.4915050323887158
2214	25	-1.8805411392456435
2215	1	-1.4909263622841564
2215	2	-0.9828539055821075
2215	3	-1.1175934564120595
2215	4	-1.1471851889641869
2215	5	-1.7313371109300526
2215	6	-1.4135455910860344
2215	7	-1.8212019059816562
2215	8	-1.535053570583429
2215	9	-1.5139709223008353
2215	10	-1.6027514753893684
2215	11	-1.3719664014099564
2215	12	-1.612465183399749
2215	13	-1.6536017636426052
2215	14	-1.9295689940954475
2215	15	-1.6895817823984984
2215	16	-1.824858352085748
2215	17	-1.9381646597760067
2215	18	-1.5720436206902235
2215	19	-1.820096041634983
2215	20	-1.8464609010918167
2215	21	-1.398564121191363
2215	22	-1.6464207318681545
2215	23	-2.2439759849208656
2215	24	-1.9945174975898468
2215	25	-2.352455096283009
2216	1	-1.627910600062222
2216	2	-0.9662185898447291
2216	3	-0.9876886133836003
2216	4	-1.3067870518797875
2216	5	-2.1155295385961637
2216	6	-2.152092248761491
2216	7	-2.3298531850601343
2216	8	-2.3254858190563006
2216	9	-4.274820512766997
2216	10	-2.299584834355535
2216	11	-2.3757135275747707
2216	12	-2.7867826598668337
2216	13	-2.5868861974132713
2216	14	-2.946212196859065
2216	15	-2.815019413766777
2216	16	-2.0515224160337624
2216	17	-2.382365526560193
2216	18	-2.874890048458866
2216	19	-3.081375080679706
2216	20	-2.3274117609748544
2216	21	-2.9663850541476933
2216	22	-2.3122734574272528
2216	23	-3.377915436220448
2216	24	-2.7265916671845716
2216	25	-2.753913013082766
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51889.0
3	0.0
4	842.0
5	7401.0
6	9075.0
7	7095.0
8	5649.0
9	4861.0
10	4175.0
11	3797.0
12	3424.0
13	3172.0
14	3100.0
15	3121.0
16	3223.0
17	3311.0
18	3710.0
19	4338.0
20	5770.0
21	7601.0
22	9539.0
23	11648.0
24	13501.0
25	15186.0
26	16949.0
27	18990.0
28	20986.0
29	25079.0
30	31643.0
31	40375.0
32	50991.0
33	66055.0
34	85473.0
35	111500.0
36	152764.0
37	236484.0
38	733794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.546591549295776	19.20377464788732	13.652619718309857	26.59701408450704
2	13.785807887435302	20.403308778602526	39.72685513422133	26.084028199740843
3	17.23383699680101	25.379324012237458	29.543077132687678	27.84376185827385
4	11.893137342653247	16.88629489459365	36.49059817801174	34.72996958474137
5	13.063039223855455	38.70104337212104	34.36546399994595	13.870453404077551
6	32.21405707790719	36.18812988155565	17.84837839558012	13.749434644957034
7	28.501702706585206	31.67265219436487	21.447624520101517	18.378020578948405
8	26.200324412003244	35.77706812652068	20.080765071640986	17.94184238983509
9	27.384014233593078	14.486340705373632	19.56730552677807	38.562339534255216
10	16.14487035298477	28.63131881716282	32.70801501890316	22.515795810949246
11	34.57777832820405	22.41853050071465	23.930463898635498	19.073227272445806
12	24.03362110843607	24.7879682724994	30.381147681709514	20.79726293735502
13	29.196197265715156	21.71660082210936	25.663446306756448	23.423755605419032
14	21.782831410075993	21.93841823810864	27.09620039403321	29.182549957782157
15	23.81863598669799	30.216582698718046	22.9982102976288	22.966571016955164
16	22.83255965295284	27.51478024137026	26.72760783580787	22.925052269869024
17	22.004538318165263	28.158288341386005	27.074541115912883	22.762632224535853
18	21.915278090520154	26.570141859941454	29.34598063499212	22.16859941454627
19	23.768433946268967	26.306939911442758	27.57664322327662	22.347982919011663
20	24.470103204205174	26.10558644689098	27.6391264062938	21.78518394261004
21	23.975982081691768	25.88033708845504	26.86445930131432	23.27922152853887
22	24.051884129272384	27.028134581707707	26.801846555327703	22.118134733692205
23	22.955106948394917	26.814976096404695	27.56746228984791	22.66245466535248
24	23.70866265393234	25.97518394200768	27.85307830911264	22.463075094947342
25	22.983871194718187	26.5073506440433	27.815870546256114	22.692907614982403
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	132.0
2	339.0
3	546.0
4	546.0
5	546.0
6	1240.5
7	1935.0
8	1935.0
9	1935.0
10	2357.5
11	2780.0
12	2780.0
13	2780.0
14	3977.0
15	5174.0
16	5174.0
17	5174.0
18	10669.0
19	16164.0
20	16164.0
21	16164.0
22	26954.0
23	37744.0
24	37744.0
25	37744.0
26	57711.5
27	77679.0
28	77679.0
29	77679.0
30	99177.0
31	120675.0
32	120675.0
33	120675.0
34	138562.0
35	156449.0
36	156449.0
37	156449.0
38	169063.5
39	181678.0
40	181678.0
41	181678.0
42	194849.5
43	208021.0
44	208021.0
45	208021.0
46	224257.0
47	240493.0
48	240493.0
49	240493.0
50	234529.5
51	228566.0
52	228566.0
53	228566.0
54	205347.5
55	182129.0
56	182129.0
57	182129.0
58	158487.5
59	134846.0
60	134846.0
61	134846.0
62	112958.0
63	91070.0
64	91070.0
65	91070.0
66	70289.0
67	49508.0
68	49508.0
69	49508.0
70	37335.0
71	25162.0
72	25162.0
73	25162.0
74	17741.5
75	10321.0
76	10321.0
77	10321.0
78	7072.0
79	3823.0
80	3823.0
81	3823.0
82	2522.5
83	1222.0
84	1222.0
85	1222.0
86	769.5
87	317.0
88	317.0
89	317.0
90	192.0
91	67.0
92	67.0
93	67.0
94	38.0
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08505435654493555
2	0.042217582666248615
3	0.018294285822041068
4	0.005629011022166483
5	0.019532668246917694
6	0.06068073881895468
7	0.07858099386944409
8	0.05578349922966984
9	0.024261037505537538
10	0.049647877215508375
11	0.0061919121243831305
12	4.503208817733186E-4
13	0.005910461573274807
14	6.191912124383131E-4
15	0.01339704623275623
16	0.008612386863914719
17	0.004615789038176515
18	0.006248202234604796
19	0.001857573637314939
20	0.001857573637314939
21	2.251604408866593E-4
22	7.880615431033076E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1776511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.50914141693561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49322005474784	41.35054686621903
2	14.389842192780486	15.975355704928779
3	4.9589404658128995	8.257995827849186
4	2.262336312597775	5.023213852346343
5	1.1778911511853927	3.2691863242453523
6	0.715434908241667	2.382790649771943
7	0.44192640875718514	1.717166886370676
8	0.313818157743355	1.393582119790248
9	0.2160021738722443	1.0791085694255929
>10	0.9296202590683629	8.781960655112497
>50	0.056755893271608036	2.157907071867153
>100	0.03689497178470944	3.9506887336048595
>500	0.004979659120623687	1.8751763833086121
>1k	0.002134139623124437	2.071432170966344
>5k	2.0325139267851782E-4	0.7138881841933301
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7307	0.41131183538970484	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5348	0.3010395094654635	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4338	0.24418649814158203	No Hit
GTCCTACAGTGGACATTTCTAAATT	3080	0.17337353948272766	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2725	0.15339055035403665	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2656	0.14950653274874176	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2330	0.13115595681647904	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2291	0.12896064251783412	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2199	0.12378195237744095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	5.629011022166483E-5	0.0	0.0
8	0.0	0.0	5.629011022166483E-5	0.0	0.0
9	0.0	0.0	5.629011022166483E-5	0.0	0.0
10	0.0	0.0	5.629011022166483E-5	0.0	0.0
11	0.0	0.0	5.629011022166483E-5	0.0	0.0
12	0.0	0.0	5.629011022166483E-5	0.0	0.0
13	0.0	0.0	5.629011022166483E-5	0.0	5.629011022166483E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTT	45	6.7995745E-4	14.767269	15
TCGTCCG	40	0.005291605	14.245079	10
GGTCGGT	55	1.9717941E-4	13.8087435	11
CGACCAT	50	0.0015036025	13.295407	10
GGTATCA	1665	0.0	12.854761	1
TCGAACT	120	7.4942363E-10	12.657302	19
CCGATCG	55	0.0030727992	12.088776	9
CCGACCA	55	0.0030727992	12.088776	9
TAGGACC	445	0.0	11.752742	4
CGCGCCT	185	0.0	11.289264	12
GCGCATT	60	0.005906177	11.075763	11
GTCCTAA	810	0.0	11.039225	1
TTAGGAC	1020	0.0	11.0022	3
GGCGAGG	730	0.0	10.923426	19
AGGACGT	1640	0.0	10.84262	5
GGACGTG	1635	0.0	10.759156	6
TCGCGCG	180	3.6379788E-11	10.553694	9
ATCGCGC	190	9.094947E-12	10.500514	8
GTCCTAC	1565	0.0	10.4546585	1
TAGGACG	1745	0.0	10.299186	4
>>END_MODULE