FastQCFastQC Report
Thu 26 May 2016
SRR1512908_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512908_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60050.3445298358247918No Hit
GTCCTACAGTGGACATTTCTAAATT41000.2352326939020227No Hit
GTCCTAAAGTGTGTATTTCTCATTT38400.2203154986789676No Hit
TATCAACGCAGAGTACTTTTTTTTT34660.19885768708888066No Hit
GGTATCAACGCAGAGTACTTTTTTT34450.19765283670548006No Hit
GTACTGGTTCACTATCGGTCAGTCA33130.19007949143839056No Hit
CTGTAGGACGTGGAATATGGCAAGA33010.18939100550501878No Hit
CTTTAGGACGTGAAATATGGCGAGG32060.18394049186582556No Hit
GGATACCACGTGTCCCGCCCTACTC18610.10677269350040591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1155.335096E-912.38973119
TAGGACC6950.012.3005964
GGTATCA17700.012.2171461
ATCCCGT1052.7269016E-711.75973610
GTGTTAT1406.493792E-1011.5674621
AAATCGC752.0765357E-411.39855317
ATCGCCG752.0765357E-411.39855319
GCACCGT1104.977064E-711.2258486
GGCGAGG9450.011.05679719
CCTAGAC1553.1286618E-1011.0308583
TGTAGGA19300.010.975292
CGAAATC1502.1391315E-910.76468213
CTGTAGG19200.010.7169141
GACAGCG803.7755934E-410.6861437
GTATCAA37050.010.6188891
GTAGGAC19050.010.5708363
CTAGACC909.5279196E-510.5542164
ACCGTGC909.5279196E-510.5542168
TGGCGAG19600.010.46805918
GTCCTAA10850.010.448031
Produced by FastQC (version 0.11.5)