##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512908_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1742955 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.056158076370302 31.0 30.0 34.0 23.0 34.0 2 29.126516748854677 31.0 30.0 34.0 22.0 34.0 3 29.29836283782427 31.0 30.0 34.0 23.0 34.0 4 32.72464464085418 35.0 33.0 37.0 25.0 37.0 5 32.92462570749101 35.0 35.0 37.0 26.0 37.0 6 32.79943486779636 36.0 33.0 37.0 25.0 37.0 7 32.78047224397646 37.0 33.0 37.0 25.0 37.0 8 32.667323596994756 37.0 33.0 37.0 25.0 37.0 9 33.96981333425131 39.0 34.0 39.0 23.0 39.0 10 33.8396080220086 38.0 34.0 39.0 23.0 39.0 11 33.899166071413205 38.0 34.0 39.0 23.0 39.0 12 33.80756703414603 38.0 34.0 39.0 22.0 39.0 13 33.77478649764337 38.0 34.0 39.0 22.0 39.0 14 34.631561342662316 39.0 34.0 41.0 19.0 41.0 15 34.8077747274026 39.0 34.0 41.0 22.0 41.0 16 34.74803480296393 39.0 34.0 41.0 22.0 41.0 17 34.51394212701992 39.0 34.0 41.0 18.0 41.0 18 34.51523992300432 39.0 34.0 41.0 18.0 41.0 19 34.633175268437796 39.0 34.0 41.0 18.0 41.0 20 34.609905591366385 39.0 34.0 41.0 18.0 41.0 21 34.51519631889521 39.0 34.0 41.0 18.0 41.0 22 34.520657733561684 39.0 34.0 41.0 18.0 41.0 23 34.18555786007097 39.0 34.0 41.0 15.0 41.0 24 34.24621978192208 39.0 34.0 41.0 15.0 41.0 25 33.95583420111248 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5746591651411173 1101 2 0.29290771570338947 1101 3 0.6194937479291553 1101 4 0.7323195694015538 1101 5 0.6428118892720533 1101 6 0.6851478693042452 1101 7 0.3571368345436525 1101 8 0.705999496476494 1101 9 0.8458726472760745 1101 10 0.9000388345639507 1101 11 0.9215536495603587 1101 12 0.7098687673222415 1101 13 1.0458377371178642 1101 14 1.078880137966884 1101 15 0.9196469626071391 1101 16 0.8283169441973044 1101 17 0.2853587486737226 1101 18 0.6823654496507103 1101 19 0.08006319846268184 1101 20 -0.6029859466309802 1101 21 0.6210707011622034 1101 22 0.6764558998734813 1101 23 0.07400160245153842 1101 24 0.6122984891373449 1101 25 0.18791135004123305 1102 1 0.8812765202537207 1102 2 0.9231979486282924 1102 3 0.9278097972296031 1102 4 1.0713584383455483 1102 5 0.9771597745116267 1102 6 0.8023441631633474 1102 7 0.7462088832776814 1102 8 0.650627841179805 1102 9 0.9920386461821096 1102 10 1.0089762760828407 1102 11 0.8596312763862883 1102 12 0.8176386689490229 1102 13 0.9435631861556502 1102 14 1.1393817497550103 1102 15 1.0673289586144605 1102 16 0.9527811391778513 1102 17 1.1407570588644944 1102 18 1.1257773871560914 1102 19 0.8557742038797329 1102 20 0.9731787472958686 1102 21 1.1935728460482125 1102 22 0.9516021547220319 1102 23 0.8879751530222606 1102 24 0.30335466155008817 1102 25 0.7076828953864833 1103 1 0.6165318487369831 1103 2 0.7911632606665329 1103 3 0.8295388123315703 1103 4 0.939515384685258 1103 5 0.7000339660544199 1103 6 0.39127593650733417 1103 7 0.3530996436513689 1103 8 0.5881405248405613 1103 9 0.6867049145078141 1103 10 0.9141113755738743 1103 11 0.5995539228775257 1103 12 0.7050206806247203 1103 13 0.8945319890603045 1103 14 0.9366523067056534 1103 15 0.8792380076830639 1103 16 0.8780071573023633 1103 17 0.4898057506643241 1103 18 0.9017329344577334 1103 19 0.8199418192976822 1103 20 1.0818744228740655 1103 21 0.8189918442206903 1103 22 0.9611042016024598 1103 23 0.31507310452693815 1103 24 0.535992782242019 1103 25 0.864514577488869 1104 1 1.1415608398980908 1104 2 0.9945407376247815 1104 3 1.0502494449569681 1104 4 1.2510147748942444 1104 5 1.063022032987476 1104 6 0.9747306126331239 1104 7 1.148280501939574 1104 8 1.017953552588061 1104 9 1.2313590621302168 1104 10 1.2617056309625951 1104 11 1.0575160703479511 1104 12 1.2397225787096176 1104 13 1.335814920852009 1104 14 1.6201440144576296 1104 15 1.3870748523316507 1104 16 1.4074711138105442 1104 17 1.6220651133243535 1104 18 1.556567943044044 1104 19 0.9474373736295547 1104 20 1.1421155672583438 1104 21 1.240692965818745 1104 22 1.154106669128879 1104 23 1.5037313127848861 1104 24 1.4075171206361574 1104 25 0.7916947208312237 1105 1 0.5277997212935901 1105 2 0.5384694375877217 1105 3 0.6480899607299264 1105 4 0.8420607807401552 1105 5 0.6183146969778832 1105 6 0.7515610374281607 1105 7 0.7178654251706078 1105 8 0.7379307840782303 1105 9 0.5547370460918728 1105 10 0.6941957177969087 1105 11 0.7914109806462122 1105 12 0.8062401073651557 1105 13 0.8629721357036573 1105 14 0.8947766159307449 1105 15 0.8701818888904853 1105 16 0.7559203561595425 1105 17 1.1685440572585009 1105 18 1.014440326187227 1105 19 0.660022848986273 1105 20 0.8384859549531001 1105 21 0.6829987686917178 1105 22 0.8014710966428922 1105 23 0.957820246669705 1105 24 1.0929739485726486 1105 25 1.31586650560687 1106 1 0.6400255453839989 1106 2 0.6345643094913029 1106 3 0.5284293045449147 1106 4 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2214 6 -1.361259289058772 2214 7 -1.1559071658707083 2214 8 -0.8852026404230422 2214 9 -0.8943922340117112 2214 10 -0.8783642735707673 2214 11 -0.832233336662263 2214 12 -0.7316155081615818 2214 13 -1.0605112063280657 2214 14 -0.9774115467709947 2214 15 -1.3304916307865966 2214 16 -1.1098607262725722 2214 17 -3.2627749930974765 2214 18 -3.2248777200001264 2214 19 -1.9489173347149276 2214 20 -1.708374696831612 2214 21 -1.6205266118903907 2214 22 -2.5545391608514834 2214 23 -1.6030040744704692 2214 24 -2.6008740872426976 2214 25 -2.599358320260162 2215 1 -1.7401009560908776 2215 2 -3.331993184288624 2215 3 -1.894891832021326 2215 4 -3.3982313972176073 2215 5 -1.9908922020975233 2215 6 -1.9126729814215082 2215 7 -2.1348241896228792 2215 8 -1.5380883420714113 2215 9 -3.9484335685959024 2215 10 -2.4091220836717078 2215 11 -1.8252581255803548 2215 12 -1.848399935571102 2215 13 -1.8040149264969862 2215 14 -1.4807753344973946 2215 15 -1.7081292037883955 2215 16 -1.7703362204873656 2215 17 -2.10261221281705 2215 18 -1.6436447819259357 2215 19 -1.7661495628439638 2215 20 -1.8520550581148143 2215 21 -2.21816449478888 2215 22 -2.3504854303996296 2215 23 -2.1478903564708247 2215 24 -2.0286442115112706 2215 25 -3.204942940369076 2216 1 -3.122860099855693 2216 2 -3.3001199928521316 2216 3 -4.62624623751228 2216 4 -5.0775481484705125 2216 5 -4.904143275136402 2216 6 -3.87226214224804 2216 7 -5.368759956092639 2216 8 -3.0648382514175054 2216 9 -4.173378923431908 2216 10 -3.007802942911834 2216 11 -3.4489723208987115 2216 12 -3.588667028708418 2216 13 -3.1974551953237444 2216 14 -4.042388208363501 2216 15 -3.5043061371003468 2216 16 -3.789364006090512 2216 17 -3.3816934806137446 2216 18 -3.1702909629765976 2216 19 -3.871614801585185 2216 20 -4.5020169906971965 2216 21 -4.266845281794819 2216 22 -3.867046129511003 2216 23 -4.023311951315037 2216 24 -4.187941546214162 2216 25 -4.377512584962421 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79124.0 3 1.0 4 750.0 5 4405.0 6 5729.0 7 5357.0 8 4627.0 9 4279.0 10 3807.0 11 3518.0 12 3360.0 13 3237.0 14 3281.0 15 3271.0 16 3351.0 17 3629.0 18 4081.0 19 5072.0 20 6504.0 21 8379.0 22 10674.0 23 12928.0 24 14963.0 25 17143.0 26 19065.0 27 20938.0 28 23623.0 29 28088.0 30 33853.0 31 42096.0 32 52781.0 33 67270.0 34 86461.0 35 113675.0 36 154963.0 37 236950.0 38 651722.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81802989054564 18.217217373383125 12.674266048998572 24.290486687072665 2 15.162309456948716 19.91976047491884 41.55252923199804 23.365400836134413 3 19.393332568280925 25.707596970392473 28.60110167546477 26.29796878586183 4 12.514344930157403 16.32573549325903 37.43080005692068 33.72911951966288 5 13.383816648821348 38.84621696464332 34.12713440910192 13.642831977433417 6 32.05930669141033 35.53629985455827 18.506813597451984 13.897579856579414 7 28.989177842137053 31.604800610009576 21.35963693405879 18.04638461379458 8 26.180262933458 35.82153927088257 20.349517373353223 17.648680422306214 9 28.151000903805052 14.546150904436683 19.693820247300355 37.60902794445791 10 17.030402183044764 28.1817550150136 32.515744326041776 22.272098475899853 11 34.573620797965866 22.336486593571667 24.32456798190962 18.765324626552847 12 24.795398879582383 24.42217519518295 30.363661945139164 20.418763980095502 13 29.525649712323577 21.946954993856014 25.486638262687045 23.040757031133367 14 22.015388405349363 22.403457826018798 26.893589848866544 28.687563919765296 15 23.933466819254818 30.668380890803192 23.086097270926846 22.31205501901514 16 22.609656350955447 27.448856895777514 27.31000995701372 22.631476796253313 17 21.960437490488403 28.309620139034454 27.04211520736004 22.687827163117106 18 21.90933383564956 26.533093431392196 29.664434001218652 21.89313873173959 19 24.028711123464262 26.272090670090837 27.344881488228072 22.354316718216825 20 24.89600202138534 26.092581745512177 27.64721083278779 21.36420540031469 21 24.299796849502115 25.91742144713436 26.535141361280594 23.247640342082928 22 24.58091960214857 27.431682816914027 26.450779984298663 21.536617596638745 23 23.159180495065883 27.178317437565468 27.403258632412665 22.259243434955987 24 24.09402490279434 26.085972811702696 27.714307535670784 22.10569474983218 25 23.334665554950696 26.68106844173713 27.756205740956396 22.228060262355783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 249.0 2 585.5 3 922.0 4 922.0 5 922.0 6 1576.0 7 2230.0 8 2230.0 9 2230.0 10 2535.5 11 2841.0 12 2841.0 13 2841.0 14 3963.0 15 5085.0 16 5085.0 17 5085.0 18 10117.5 19 15150.0 20 15150.0 21 15150.0 22 25506.5 23 35863.0 24 35863.0 25 35863.0 26 55126.0 27 74389.0 28 74389.0 29 74389.0 30 98018.0 31 121647.0 32 121647.0 33 121647.0 34 138225.0 35 154803.0 36 154803.0 37 154803.0 38 166358.0 39 177913.0 40 177913.0 41 177913.0 42 190993.5 43 204074.0 44 204074.0 45 204074.0 46 221049.5 47 238025.0 48 238025.0 49 238025.0 50 231747.0 51 225469.0 52 225469.0 53 225469.0 54 201119.5 55 176770.0 56 176770.0 57 176770.0 58 153645.0 59 130520.0 60 130520.0 61 130520.0 62 109889.0 63 89258.0 64 89258.0 65 89258.0 66 68859.0 67 48460.0 68 48460.0 69 48460.0 70 36066.5 71 23673.0 72 23673.0 73 23673.0 74 16897.5 75 10122.0 76 10122.0 77 10122.0 78 6954.5 79 3787.0 80 3787.0 81 3787.0 82 2513.0 83 1239.0 84 1239.0 85 1239.0 86 790.0 87 341.0 88 341.0 89 341.0 90 217.5 91 94.0 92 94.0 93 94.0 94 57.0 95 20.0 96 20.0 97 20.0 98 15.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3375876026632931 2 0.00992567220610974 3 0.008892943306052078 4 0.010269915172795626 5 0.011532139383977211 6 0.0782579010932583 7 0.07705305070985768 8 0.10063369392784094 9 0.08198719989902206 10 0.08801145181602509 11 0.08474114363250916 12 0.09409307756080909 13 0.08118396631008833 14 0.08686397526040546 15 0.0830773026268607 16 0.0842821530102613 17 0.09524055411642872 18 0.09684702129429618 19 0.09231448889959867 20 0.09036377875504531 21 0.07969224678778282 22 0.09765025488322991 23 0.09242923655516062 24 0.08858519009383489 25 0.08623286315481467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1742955.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.95020288936474 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13982849602868 37.264891011660026 2 14.197231692245568 14.467036703744615 3 5.372136478855804 8.211343306411823 4 2.5119339481812344 5.119341772180676 5 1.3778952031115206 3.51020200794072 6 0.8405963422173184 2.5697132510418155 7 0.544001820133418 1.9401902175586943 8 0.3794654576216531 1.546707364426309 9 0.2852046474946471 1.3078111189357815 >10 1.2151162781758729 10.624778190067383 >50 0.07436954583493686 2.6494459671697244 >100 0.0525697214946303 5.139503995379637 >500 0.006357889904330469 2.285771851674449 >1k 0.0031789449521652346 3.015899962817936 >5k 1.1353374829161552E-4 0.34736327899038505 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6005 0.3445298358247918 No Hit GTCCTACAGTGGACATTTCTAAATT 4100 0.2352326939020227 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3840 0.2203154986789676 No Hit TATCAACGCAGAGTACTTTTTTTTT 3466 0.19885768708888066 No Hit GGTATCAACGCAGAGTACTTTTTTT 3445 0.19765283670548006 No Hit GTACTGGTTCACTATCGGTCAGTCA 3313 0.19007949143839056 No Hit CTGTAGGACGTGGAATATGGCAAGA 3301 0.18939100550501878 No Hit CTTTAGGACGTGAAATATGGCGAGG 3206 0.18394049186582556 No Hit GGATACCACGTGTCCCGCCCTACTC 1861 0.10677269350040591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 5.737382778098115E-5 5.737382778098115E-5 13 0.0 0.0 0.0 5.737382778098115E-5 5.737382778098115E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 115 5.335096E-9 12.389731 19 TAGGACC 695 0.0 12.300596 4 GGTATCA 1770 0.0 12.217146 1 ATCCCGT 105 2.7269016E-7 11.759736 10 GTGTTAT 140 6.493792E-10 11.567462 1 AAATCGC 75 2.0765357E-4 11.398553 17 ATCGCCG 75 2.0765357E-4 11.398553 19 GCACCGT 110 4.977064E-7 11.225848 6 GGCGAGG 945 0.0 11.056797 19 CCTAGAC 155 3.1286618E-10 11.030858 3 TGTAGGA 1930 0.0 10.97529 2 CGAAATC 150 2.1391315E-9 10.764682 13 CTGTAGG 1920 0.0 10.716914 1 GACAGCG 80 3.7755934E-4 10.686143 7 GTATCAA 3705 0.0 10.618889 1 GTAGGAC 1905 0.0 10.570836 3 CTAGACC 90 9.5279196E-5 10.554216 4 ACCGTGC 90 9.5279196E-5 10.554216 8 TGGCGAG 1960 0.0 10.468059 18 GTCCTAA 1085 0.0 10.44803 1 >>END_MODULE