FastQCFastQC Report
Thu 26 May 2016
SRR1512908_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512908_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742955
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79930.4585890054533823No Hit
TATCAACGCAGAGTACTTTTTTTTT58770.3371859858688262No Hit
GGTATCAACGCAGAGTACTTTTTTT45770.26260000975355074No Hit
GTCCTACAGTGGACATTTCTAAATT39030.22393004982916945No Hit
GTCCTAAAGTGTGTATTTCTCATTT36010.2066031538393131No Hit
CTTTAGGACGTGAAATATGGCGAGG32510.18652231411596973No Hit
CTGTAGGACGTGGAATATGGCAAGA32110.18422736100473047No Hit
GTACTGGTTCACTATCGGTCAGTCA29560.16959703492058026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27790.15944186740334662No Hit
CTGAAGGACCTGGAATATGGCGAGA17800.10212541345014645No Hit
GGATACCACGTGTCCCGCCCTACTC17440.10005995565003113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC957.261406E-813.0172913
TATGTCG905.45675E-712.6569516
GGTATCA16300.012.493651
ATCGCAC701.09184715E-412.21332558
TAGGACC5850.011.5409374
TCCAACG1657.2759576E-1211.50565818
ATAGGAC2150.011.5036523
ATTTAGA4800.011.4987331
CTAAGAC1501.72804E-1011.4151633
TAGAAAT5000.011.4108984
GAGCGTC600.0058494911.0901037
GGCGAGG9150.010.99639119
GTATCAA35750.010.9402941
TGTCGAG1158.9110836E-710.73027718
TAAGACC1355.6077624E-810.5656474
GGCGTGC1355.693437E-810.5547278
ATTATAC1451.4135367E-810.4967023
AGGACCT12150.010.4094175
TTTAGAA5700.010.3470772
ATGTCGA1201.5392634E-610.28347717
Produced by FastQC (version 0.11.5)