FastQCFastQC Report
Thu 26 May 2016
SRR1512907_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512907_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334640
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58100.43532338308457713No Hit
TATCAACGCAGAGTACTTTTTTTTT34230.2564736558172991No Hit
GGTATCAACGCAGAGTACTTTTTTT33250.24913085176527006No Hit
GTACTGGTTCACTATCGGTCAGTCA30000.22477971587843912No Hit
GTCCTACAGTGGACATTTCTAAATT23190.17375472037403344No Hit
GTCCTAAAGTGTGTATTTCTCATTT21420.16049271713720553No Hit
CTGTAGGACGTGGAATATGGCAAGA19880.14895402505544567No Hit
GGATACCACGTGTCCCGCCCTACTC16820.12602649403584487No Hit
CTTTAGGACGTGAAATATGGCGAGG16320.12228016543787089No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15430.1156117005334772No Hit
ATCATTAACTGAATCCATAGGTTAA13750.10302403644428461No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13420.10055145956962178No Hit
GTCCTACAGTGTGCATTTCTCATTT13410.1004765329976623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.012.8567341
GCTAGAT604.0944165E-412.6655423
GTACTAA701.06849104E-412.24119951
ACCTTCG550.00307095712.08938212
GTCCTAG2000.011.9011671
TAGGACC4200.011.760864
CGTGCGC1052.7243368E-711.75997810
GTCCTAA6600.011.6847821
TCCCGCC3850.011.59593813
CGCCCTA3900.011.44684116
TCTATAC1001.9307736E-611.3989883
GTAGCAC1354.7293724E-911.258263
GCGCCAC1104.973663E-711.22585513
GTATCAA30200.011.0657211
TTAGGAC8100.010.9064393
GATTAAG1053.374218E-610.8810671
CGGTAGC700.001464026210.8810661
CCCGCCC4150.010.75767714
GAACAGT1605.4387783E-1010.6865516
GTCCCGC4650.010.62231512
Produced by FastQC (version 0.11.5)