##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512907_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334640 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69668824551939 31.0 30.0 34.0 20.0 34.0 2 28.85706332793862 31.0 30.0 34.0 16.0 34.0 3 28.96474180303303 31.0 30.0 34.0 20.0 34.0 4 32.360020679733864 35.0 33.0 37.0 22.0 37.0 5 32.530080021578854 35.0 33.0 37.0 25.0 37.0 6 32.4386853383684 36.0 33.0 37.0 23.0 37.0 7 32.36403749325661 36.0 33.0 37.0 22.0 37.0 8 32.267411436791946 37.0 33.0 37.0 20.0 37.0 9 33.525317688665105 38.0 34.0 39.0 18.0 39.0 10 33.40088263501768 38.0 33.0 39.0 17.0 39.0 11 33.44796499430558 38.0 34.0 39.0 17.0 39.0 12 33.34539800995025 38.0 34.0 39.0 17.0 39.0 13 33.302471078343224 38.0 33.0 39.0 17.0 39.0 14 34.14253731343283 39.0 33.0 41.0 17.0 41.0 15 34.30999970029371 39.0 34.0 41.0 17.0 41.0 16 34.25028322244201 39.0 34.0 41.0 17.0 41.0 17 34.01830755859258 39.0 33.0 41.0 12.0 41.0 18 34.02698555415692 39.0 33.0 41.0 15.0 41.0 19 34.15185668045316 39.0 34.0 41.0 15.0 41.0 20 34.11931607025115 39.0 34.0 41.0 11.0 41.0 21 34.01094527363184 39.0 34.0 41.0 10.0 41.0 22 34.01972367080261 39.0 34.0 41.0 9.0 41.0 23 33.68102184858839 39.0 33.0 41.0 2.0 41.0 24 33.74561904333753 39.0 34.0 41.0 2.0 41.0 25 33.464248786189536 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33044857319273646 1101 2 0.15646871694539755 1101 3 0.3987352331372449 1101 4 0.3683957939401523 1101 5 0.34059136753392494 1101 6 0.3755376639580774 1101 7 0.12673529697035946 1101 8 0.3192022603218021 1101 9 0.49274755156110217 1101 10 0.5694543631552378 1101 11 0.5765454323579249 1101 12 0.47659365109574026 1101 13 0.6901148367676342 1101 14 0.6033689384363399 1101 15 0.5480996316877977 1101 16 0.4183611520960042 1101 17 -0.19460975502131106 1101 18 0.12724593478234425 1101 19 -0.23204998429556412 1101 20 -0.816477060315421 1101 21 0.3156049308168889 1101 22 0.44295502185094904 1101 23 -0.11219292963388483 1101 24 0.37433439867455576 1101 25 0.10152609192199691 1102 1 1.1480192992495617 1102 2 1.1630716612347847 1102 3 1.0754014163409593 1102 4 1.1517493477848575 1102 5 1.1484890778795105 1102 6 1.0494076836363249 1102 7 1.1524608343982337 1102 8 1.1659767620936279 1102 9 1.5650362063842351 1102 10 1.5387465439912873 1102 11 1.288836056965927 1102 12 1.5346435052728609 1102 13 1.3597537389408672 1102 14 1.4928110875206357 1102 15 1.3774483972892781 1102 16 1.2888451017792733 1102 17 1.5938684244662724 1102 18 1.4885115852621453 1102 19 1.360144767648741 1102 20 1.7000573727001367 1102 21 1.7016129412753713 1102 22 1.6277730274486686 1102 23 1.479284957815942 1102 24 0.8482455290370723 1102 25 1.2431590141811526 1103 1 0.8683917901116942 1103 2 1.0410483124702843 1103 3 1.195903281674454 1103 4 1.1992355843423752 1103 5 0.9982535040062643 1103 6 0.6400002574189472 1103 7 0.5350847714602196 1103 8 0.8664649489441629 1103 9 0.9209349459625358 1103 10 1.0895860856629866 1103 11 1.020498252244444 1103 12 1.0088696184313903 1103 13 1.2223522447860802 1103 14 1.1759941998333474 1103 15 1.1171534272705514 1103 16 1.1695196898199924 1103 17 0.935967613763637 1103 18 1.2495296552083772 1103 19 1.11154093578552 1103 20 1.3373746153294235 1103 21 1.0672317581101964 1103 22 1.1937485798743595 1103 23 0.6694701506929377 1103 24 0.702567797494055 1103 25 1.131130917123393 1104 1 0.9815454410764843 1104 2 0.9355551347924767 1104 3 0.9809002144178827 1104 4 1.1061213429771684 1104 5 0.8876191860525893 1104 6 0.7735137533478635 1104 7 1.0287604137251591 1104 8 0.7694548269974746 1104 9 1.2587711823457752 1104 10 1.1897306185105165 1104 11 1.0188015016774656 1104 12 1.242005284119017 1104 13 1.4415971884601007 1104 14 1.5175406548283306 1104 15 1.2480388420008168 1104 16 1.259465594567736 1104 17 1.5048735326393512 1104 18 1.2709860443967642 1104 19 0.8407637580333542 1104 20 1.027220834238598 1104 21 1.1565783859869114 1104 22 0.9612691211986686 1104 23 1.4767158472390207 1104 24 1.4256944473063058 1104 25 0.8544420670657686 1105 1 0.8052975403061602 1105 2 0.8815720746632358 1105 3 0.9435909705056496 1105 4 1.1076038098458412 1105 5 0.7295588286359163 1105 6 1.038451597895346 1105 7 1.071829076789669 1105 8 0.9812477060453375 1105 9 0.6820591692757887 1105 10 0.8709393184484568 1105 11 0.8771185583749173 1105 12 1.1099199276150529 1105 13 1.115743121430782 1105 14 1.1981209549268996 1105 15 1.1663048782901058 1105 16 1.010539852535679 1105 17 1.2573346512549506 1105 18 1.0527359805071654 1105 19 0.9036094463981499 1105 20 1.2112774130418558 1105 21 1.0098023007764425 1105 22 1.0372064728835255 1105 23 1.2444237622475995 1105 24 1.4762197110790751 1105 25 1.6990004986673881 1106 1 0.8898411872831744 1106 2 0.8866723688832145 1106 3 0.6646069027246675 1106 4 0.9871741285135158 1106 5 0.7191786900912405 1106 6 1.0196081520796483 1106 7 0.9863201442301772 1106 8 1.0249631496365623 1106 9 1.1446533632169107 1106 10 1.0268097685067445 1106 11 0.7234336825486025 1106 12 0.9826144786536233 1106 13 0.864563237376835 1106 14 1.3163762402438905 1106 15 0.9147048795942325 1106 16 1.1557043656102337 1106 17 1.3260981495337418 1106 18 1.2002162474616043 1106 19 1.313241017035395 1106 20 1.4239441489513638 1106 21 1.3269306355528485 1106 22 1.261563527479339 1106 23 1.3324105328282059 1106 24 1.1828422921013484 1106 25 1.2839053417694757 1107 1 1.0971128841967435 1107 2 1.0448906349730471 1107 3 0.9812387842987071 1107 4 1.18231988035528 1107 5 0.9499273297867887 1107 6 1.0920357829614389 1107 7 0.8987879507421326 1107 8 1.0815462860079492 1107 9 1.1090126955274258 1107 10 1.2456397914971546 1107 11 1.1741779913849442 1107 12 1.3128070175968034 1107 13 1.0593901642636396 1107 14 1.5014066714408258 1107 15 1.1606336415674505 1107 16 1.2379506072076794 1107 17 1.5743706435097025 1107 18 1.5092986121107401 1107 19 1.2228850111933411 1107 20 1.4677070205874614 1107 21 1.1205522935347645 1107 22 1.279109496785523 1107 23 1.6507904228981047 1107 24 1.4362984559736915 1107 25 1.5611231793806866 1108 1 0.9802035926264274 1108 2 0.8124638603952334 1108 3 0.8768531420872279 1108 4 1.0058917218520236 1108 5 0.8864423777862243 1108 6 0.9717126626475192 1108 7 1.0237805055736047 1108 8 1.017517398754073 1108 9 1.0127536737349843 1108 10 1.0649315369950187 1108 11 0.8867765303801036 1108 12 0.8900248019146488 1108 13 1.0037384054520615 1108 14 1.1095613207295898 1108 15 1.0819653632407693 1108 16 0.790557730044199 1108 17 1.030748963178958 1108 18 1.1674484439375163 1108 19 1.096647799365158 1108 20 1.15924580553596 1108 21 1.130243713197011 1108 22 1.072276583885234 1108 23 1.2395315762860903 1108 24 1.1608767090743868 1108 25 1.3902701382483187 1109 1 0.8591757928263526 1109 2 0.8165730775887106 1109 3 0.900635069631953 1109 4 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-1.8106882877511694 2215 17 -2.0956684911572587 2215 18 -1.7508336908750195 2215 19 -1.9326339038956846 2215 20 -1.806017791850877 2215 21 -2.5350772051309534 2215 22 -2.3979222281196186 2215 23 -2.310180196303712 2215 24 -2.3570552724145024 2215 25 -2.9362598966968463 2216 1 -3.0529623636487386 2216 2 -3.2809739279033323 2216 3 -4.581581356478878 2216 4 -4.970350912007909 2216 5 -4.893463569835511 2216 6 -3.856460722344149 2216 7 -5.573500146150998 2216 8 -3.2020134657304027 2216 9 -4.048242703983519 2216 10 -3.027005310869157 2216 11 -3.147708671187523 2216 12 -3.5779405343174595 2216 13 -3.240402876041763 2216 14 -4.087476668528719 2216 15 -3.644603338530679 2216 16 -4.007307653746004 2216 17 -3.474894537096379 2216 18 -3.0338990501445373 2216 19 -3.9892081836569595 2216 20 -4.387291393471401 2216 21 -4.1066525583203415 2216 22 -3.6841549324668783 2216 23 -3.624458547364938 2216 24 -4.0180898768984825 2216 25 -4.294196022204808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 71895.0 3 3.0 4 624.0 5 3961.0 6 5157.0 7 4864.0 8 4099.0 9 3670.0 10 3288.0 11 3036.0 12 2900.0 13 2736.0 14 2613.0 15 2709.0 16 2848.0 17 2975.0 18 3414.0 19 3986.0 20 5319.0 21 6925.0 22 8530.0 23 10321.0 24 11902.0 25 13568.0 26 14800.0 27 16363.0 28 18413.0 29 21293.0 30 25847.0 31 32090.0 32 40459.0 33 50765.0 34 65245.0 35 85575.0 36 115695.0 37 177283.0 38 489469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88272504326671 18.39477882479953 12.789879424594137 23.932616707339626 2 15.477486645000932 20.072550484481674 40.75423429715126 23.695728573366136 3 19.630998964423217 25.37088185882286 28.64313964101004 26.354979535743883 4 12.661699501759838 16.173059961918234 37.14088529236109 34.024355243960834 5 13.597175835136156 38.453168755240796 33.965887913146894 13.983767496476162 6 32.19607922535344 35.37748122885833 18.343790282123905 14.082649263664335 7 28.988291543125726 31.683696195013972 21.129887834572866 18.19812442728744 8 26.363946611463007 35.2487352287089 20.559387059392684 17.827931100435404 9 28.043575095574553 14.498385468768982 19.67613388385874 37.78190555179773 10 17.11445106819547 28.124210205809586 32.25585094535318 22.505487780641765 11 34.832902378152674 22.510633894075017 23.760153943989827 18.896309783782485 12 24.52697310789243 24.523523094092376 30.143370573482294 20.8061332245329 13 29.65425960712853 21.752436230563585 25.539045305077185 23.054258857230703 14 22.021690971894127 22.391181424536803 26.918583112478984 28.66854449109008 15 24.197421249420703 30.17358647903209 23.11801750862758 22.510974762919624 16 22.74923845991172 27.585299239134837 26.941646006470275 22.723816294483164 17 22.070693661301043 28.15183656815935 26.971683834570115 22.805785935969496 18 22.022463482777372 26.44038176670229 29.454013613095576 22.08314113742476 19 23.917297219866008 26.336761532767394 27.552643598506922 22.19329764885968 20 24.715929331632395 25.96665774199911 27.782190113418277 21.535222812950217 21 24.401261591206655 25.892239834307425 26.67571016996687 23.03078840451905 22 24.563929940777278 27.048944251435564 26.598473529782368 21.78865227800479 23 23.31103149681297 26.86112588150503 27.475657599186427 22.35218502249558 24 23.94649591917392 25.989421274891615 27.674322855850807 22.389759950083658 25 23.425584075966434 26.568453860754698 27.73341852709289 22.272543536185974 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 199.0 2 489.0 3 779.0 4 779.0 5 779.0 6 1506.5 7 2234.0 8 2234.0 9 2234.0 10 2388.5 11 2543.0 12 2543.0 13 2543.0 14 3411.0 15 4279.0 16 4279.0 17 4279.0 18 8219.5 19 12160.0 20 12160.0 21 12160.0 22 19623.5 23 27087.0 24 27087.0 25 27087.0 26 41686.5 27 56286.0 28 56286.0 29 56286.0 30 73037.0 31 89788.0 32 89788.0 33 89788.0 34 101891.5 35 113995.0 36 113995.0 37 113995.0 38 124097.0 39 134199.0 40 134199.0 41 134199.0 42 144333.5 43 154468.0 44 154468.0 45 154468.0 46 168081.5 47 181695.0 48 181695.0 49 181695.0 50 177597.5 51 173500.0 52 173500.0 53 173500.0 54 155127.0 55 136754.0 56 136754.0 57 136754.0 58 119342.0 59 101930.0 60 101930.0 61 101930.0 62 87057.0 63 72184.0 64 72184.0 65 72184.0 66 55349.0 67 38514.0 68 38514.0 69 38514.0 70 28792.0 71 19070.0 72 19070.0 73 19070.0 74 13745.0 75 8420.0 76 8420.0 77 8420.0 78 5778.5 79 3137.0 80 3137.0 81 3137.0 82 2065.0 83 993.0 84 993.0 85 993.0 86 668.5 87 344.0 88 344.0 89 344.0 90 204.0 91 64.0 92 64.0 93 64.0 94 38.5 95 13.0 96 13.0 97 13.0 98 9.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33851825211292935 2 0.009066115207097044 3 0.008841335491218606 4 0.010714499790205598 5 0.011763471797638314 6 0.08069591800035965 7 0.08047113828448121 8 0.1045225678834742 9 0.08436732002637415 10 0.0916351975064437 11 0.0875142360486723 12 0.09830366241083738 13 0.08429239345441467 14 0.08856320805610501 15 0.08436732002637415 16 0.08676497032907751 17 0.10025175328178386 18 0.10227477072468981 19 0.09643049811185038 20 0.094182700953066 21 0.08137025714799496 22 0.10249955044056824 23 0.0949319666726608 24 0.09058622549901096 25 0.09058622549901096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1334640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.741125379140655 #Duplication Level Percentage of deduplicated Percentage of total 1 76.83373166279208 42.82798669962942 2 13.16887021558979 14.680952915776432 3 4.37025708827082 7.3080914488914575 4 2.0060282130260543 4.472730805455151 5 1.0445944262375673 2.911343444162987 6 0.6174649392019388 2.0650914355967243 7 0.3943115958067894 1.5385560470220696 8 0.2909553263356124 1.2974541860001725 9 0.19814442794359996 0.9940314061064076 >10 0.9540728390830349 9.451673057890138 >50 0.06730991271463084 2.575869291986422 >100 0.046780023148332205 5.078234584504319 >500 0.005031549171681393 2.0301818980916737 >1k 0.002311792862664424 2.3273981629767673 >5k 1.3598781545084846E-4 0.4404046159098169 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5810 0.43532338308457713 No Hit TATCAACGCAGAGTACTTTTTTTTT 3423 0.2564736558172991 No Hit GGTATCAACGCAGAGTACTTTTTTT 3325 0.24913085176527006 No Hit GTACTGGTTCACTATCGGTCAGTCA 3000 0.22477971587843912 No Hit GTCCTACAGTGGACATTTCTAAATT 2319 0.17375472037403344 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2142 0.16049271713720553 No Hit CTGTAGGACGTGGAATATGGCAAGA 1988 0.14895402505544567 No Hit GGATACCACGTGTCCCGCCCTACTC 1682 0.12602649403584487 No Hit CTTTAGGACGTGAAATATGGCGAGG 1632 0.12228016543787089 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1543 0.1156117005334772 No Hit ATCATTAACTGAATCCATAGGTTAA 1375 0.10302403644428461 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1342 0.10055145956962178 No Hit GTCCTACAGTGTGCATTTCTCATTT 1341 0.1004765329976623 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.49265719594797E-5 5 0.0 0.0 0.0 0.0 7.49265719594797E-5 6 0.0 0.0 0.0 0.0 1.498531439189594E-4 7 0.0 0.0 0.0 0.0 1.498531439189594E-4 8 0.0 0.0 0.0 0.0 1.498531439189594E-4 9 0.0 0.0 0.0 0.0 2.2477971587843914E-4 10 0.0 0.0 0.0 0.0 2.2477971587843914E-4 11 0.0 0.0 0.0 0.0 2.2477971587843914E-4 12 0.0 0.0 0.0 0.0 2.2477971587843914E-4 13 0.0 0.0 0.0 0.0 2.2477971587843914E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1370 0.0 12.856734 1 GCTAGAT 60 4.0944165E-4 12.665542 3 GTACTAA 70 1.06849104E-4 12.2411995 1 ACCTTCG 55 0.003070957 12.089382 12 GTCCTAG 200 0.0 11.901167 1 TAGGACC 420 0.0 11.76086 4 CGTGCGC 105 2.7243368E-7 11.759978 10 GTCCTAA 660 0.0 11.684782 1 TCCCGCC 385 0.0 11.595938 13 CGCCCTA 390 0.0 11.446841 16 TCTATAC 100 1.9307736E-6 11.398988 3 GTAGCAC 135 4.7293724E-9 11.25826 3 GCGCCAC 110 4.973663E-7 11.225855 13 GTATCAA 3020 0.0 11.065721 1 TTAGGAC 810 0.0 10.906439 3 GATTAAG 105 3.374218E-6 10.881067 1 CGGTAGC 70 0.0014640262 10.881066 1 CCCGCCC 415 0.0 10.757677 14 GAACAGT 160 5.4387783E-10 10.686551 6 GTCCCGC 465 0.0 10.622315 12 >>END_MODULE