FastQCFastQC Report
Thu 26 May 2016
SRR1512907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334640
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73360.5496613318947432No Hit
TATCAACGCAGAGTACTTTTTTTTT52760.395312593658215No Hit
GGTATCAACGCAGAGTACTTTTTTT43050.3225588922855602No Hit
GTACTGGTTCACTATCGGTCAGTCA27650.20717197146796143No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25210.18888988790984834No Hit
GTCCTACAGTGGACATTTCTAAATT21290.1595186717017323No Hit
GTCCTAAAGTGTGTATTTCTCATTT19830.14857939219564828No Hit
CTGTAGGACGTGGAATATGGCAAGA19430.14558232931726908No Hit
CTTTAGGACGTGAAATATGGCGAGG18120.13576694839057724No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16430.12310435772942517No Hit
GGATACCACGTGTCCCGCCCTACTC15800.11838398369597794No Hit
ATCATTAACTGAATCCATAGGTTAA14810.11096625307198944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCCG704.390804E-714.9416635
CGAGTAA400.00528729714.24645810
GGTATCA13200.014.0474511
TAGGACC3900.012.67777254
TCCAACG1155.3732947E-912.38311918
GTATTAC1103.7614882E-812.102421
GTATAAA951.0275162E-612.0114231
CTACACG657.96334E-411.702564
GGCCGTG951.350463E-511.0067547
TTAGACA1053.4282093E-610.8666644
GTATCAA29500.010.8306391
TAGAAAT3450.010.7485484
TGGCGAG12200.010.58316918
GTATTAG909.420507E-510.5656041
GTATAAT1909.094947E-1210.5099951
CTCCTAA1002.3726225E-510.4599491
GAACAGT2750.010.3727246
CCAGAAC3300.010.37233453
GTCCTAC10800.010.3014641
GTCCTAG1951.4551915E-1110.2405081
Produced by FastQC (version 0.11.5)