##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512907_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1334640
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.09886785949769	31.0	30.0	34.0	26.0	34.0
2	30.118894982916743	31.0	30.0	34.0	26.0	34.0
3	30.297400047953005	31.0	30.0	34.0	26.0	34.0
4	33.873658814361924	37.0	35.0	37.0	30.0	37.0
5	33.43759365821495	37.0	35.0	37.0	28.0	37.0
6	33.27069546844093	37.0	35.0	37.0	27.0	37.0
7	33.10884283402266	37.0	35.0	37.0	26.0	37.0
8	33.04653464604687	37.0	35.0	37.0	26.0	37.0
9	34.33899403584487	39.0	35.0	39.0	25.0	39.0
10	34.27650152850207	39.0	35.0	39.0	25.0	39.0
11	34.24284001678355	39.0	35.0	39.0	25.0	39.0
12	34.109995204699395	39.0	35.0	39.0	25.0	39.0
13	34.090268536833904	39.0	35.0	39.0	25.0	39.0
14	35.14656087034706	39.0	35.0	41.0	25.0	41.0
15	35.13954624468021	39.0	35.0	41.0	25.0	41.0
16	35.142260834382306	39.0	35.0	41.0	25.0	41.0
17	35.14193865012288	40.0	35.0	41.0	25.0	41.0
18	35.0709734460229	40.0	35.0	41.0	24.0	41.0
19	35.08852649403585	40.0	35.0	41.0	24.0	41.0
20	35.062641611221004	40.0	35.0	41.0	24.0	41.0
21	35.018700923095366	40.0	35.0	41.0	24.0	41.0
22	34.983905023077384	40.0	35.0	41.0	23.0	41.0
23	34.82927680872745	40.0	35.0	41.0	22.0	41.0
24	34.77313208056105	40.0	35.0	41.0	21.0	41.0
25	34.7155974644848	39.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5034018643120426
1101	2	0.3434127492311525
1101	3	0.2404237591486904
1101	4	0.36614360258057843
1101	5	0.43942637281989505
1101	6	0.3757050121575958
1101	7	0.4615758695913925
1101	8	0.13520593200745878
1101	9	0.4508259567961872
1101	10	0.07659967686770841
1101	11	0.39807274751210997
1101	12	0.5096692497659845
1101	13	0.6913761620311902
1101	14	0.6560862000887582
1101	15	0.38101993811415014
1101	16	0.531013117834199
1101	17	0.6055509865077582
1101	18	0.5158845805541858
1101	19	0.45801219733972687
1101	20	0.6011401197629525
1101	21	0.5130455850412758
1101	22	0.5398158388958407
1101	23	0.7080822513543197
1101	24	0.7161115497888346
1101	25	0.7772561738532175
1102	1	0.8553152016176107
1102	2	0.9338949643277417
1102	3	0.6980388396648713
1102	4	0.6784828838149011
1102	5	0.9784531030946084
1102	6	0.9277827922002473
1102	7	0.8713624174307029
1102	8	0.9383962485058248
1102	9	1.1513532417466408
1102	10	0.9160457949215228
1102	11	1.0784606971326554
1102	12	1.1255572797459124
1102	13	1.1345490934630789
1102	14	1.201871938454289
1102	15	1.1626754100001264
1102	16	1.2063161315070516
1102	17	1.2956396366269232
1102	18	0.6498493067050859
1102	19	1.0956580202198083
1102	20	1.1632998173764761
1102	21	1.156439798773711
1102	22	0.9572404480748418
1102	23	1.1235030209228114
1102	24	1.0395776655690625
1102	25	1.1812078465043712
1103	1	0.7310534054600453
1103	2	0.9468432674028975
1103	3	0.6651776767389421
1103	4	0.4853226916931064
1103	5	0.7132647006356905
1103	6	0.7719245499938694
1103	7	1.027905291781238
1103	8	0.9282018139208787
1103	9	0.9881665328141835
1103	10	1.0623996626565386
1103	11	1.0938065107050363
1103	12	1.3478057240426722
1103	13	1.190194040070736
1103	14	1.3102665999755558
1103	15	1.1585418932800948
1103	16	1.322652148302602
1103	17	1.2166579614517161
1103	18	1.057941276331114
1103	19	1.0810298786054986
1103	20	1.0612221322779973
1103	21	1.10661316339732
1103	22	0.9964466712653959
1103	23	1.1253098267468786
1103	24	1.0882443674781968
1103	25	1.185044476896472
1104	1	0.712707028540688
1104	2	0.8301239306738957
1104	3	0.7860271209148699
1104	4	0.6915838453533638
1104	5	0.749501780979223
1104	6	0.7749883210464006
1104	7	0.7597413236807
1104	8	0.7484247941789022
1104	9	0.7637555854966394
1104	10	0.8879733790561417
1104	11	0.8755535456903445
1104	12	0.8515789143612977
1104	13	0.7045385766361534
1104	14	0.9495732605696716
1104	15	1.0722983066347425
1104	16	1.0052944968916648
1104	17	0.9959551414346137
1104	18	0.5686669148781647
1104	19	0.9771484048351908
1104	20	1.0186258390110936
1104	21	0.9919040415621723
1104	22	0.7845015658633017
1104	23	0.9995246555381954
1104	24	1.1571858386459866
1104	25	0.9638776181389872
1105	1	0.6676242458748476
1105	2	0.5805967137048178
1105	3	0.6017069975166756
1105	4	0.4868708465581406
1105	5	0.5947928893724921
1105	6	0.8349681045519333
1105	7	0.8602960223043397
1105	8	0.6264554429321407
1105	9	0.8391204407410626
1105	10	0.5924659587355947
1105	11	0.865187001935098
1105	12	0.9421118448859218
1105	13	1.0535409861867393
1105	14	0.9760904103634047
1105	15	1.0058606537560593
1105	16	0.9825326864429798
1105	17	0.6675208473234093
1105	18	0.575814791918063
1105	19	0.9342985636464789
1105	20	0.8274640522928252
1105	21	0.7257351813526114
1105	22	0.7356648129152532
1105	23	1.014852911169605
1105	24	0.9685625225932668
1105	25	0.8254626278136996
1106	1	0.3418560811563758
1106	2	0.6353553038149826
1106	3	-1.2833178251106432
1106	4	-0.11467370352563222
1106	5	0.24495499680167399
1106	6	0.5029196451197748
1106	7	0.6201620793234923
1106	8	0.6341979881096051
1106	9	0.9551135344662214
1106	10	0.8139835134261872
1106	11	0.7754440869449724
1106	12	1.0521818810707373
1106	13	1.0301915546689173
1106	14	1.0824811831181762
1106	15	1.1759826313875195
1106	16	1.0721044349908624
1106	17	1.1157978416686305
1106	18	1.0985519831955202
1106	19	1.150138515803711
1106	20	1.2400788094949746
1106	21	1.3041082198666913
1106	22	1.3441235557478066
1106	23	1.3936936183222244
1106	24	1.2408741265602288
1106	25	1.2548472716454384
1107	1	0.7283308999744165
1107	2	0.8368411571094327
1107	3	0.7576820504923347
1107	4	0.5744996091670131
1107	5	0.646118339303527
1107	6	0.728976674242503
1107	7	0.7927518870609873
1107	8	0.6771942055187381
1107	9	0.7609680268111916
1107	10	0.8722073082724648
1107	11	0.875968858077492
1107	12	1.1315762058022472
1107	13	0.9735075184924185
1107	14	1.0750224299449016
1107	15	0.918738808621022
1107	16	1.0938711491126867
1107	17	1.1229843035870175
1107	18	1.288281396640535
1107	19	1.1150682314874132
1107	20	1.2608116461616845
1107	21	1.1874371762678422
1107	22	1.2207917428224917
1107	23	1.2365935432232789
1107	24	1.2721664273061535
1107	25	1.310112721064229
1108	1	0.4864369366922361
1108	2	0.3884046424993848
1108	3	0.40654950897457454
1108	4	0.37006404053131803
1108	5	0.476281344699089
1108	6	0.47422052540920134
1108	7	0.44108460726279475
1108	8	0.3034047023304538
1108	9	0.5200055854966408
1108	10	0.6029992114327172
1108	11	0.5746637638304151
1108	12	0.48114263422352366
1108	13	0.5458057982205418
1108	14	0.6629989207304092
1108	15	0.5423614524441547
1108	16	0.4957867471786912
1108	17	0.5330317774851352
1108	18	0.6976207036267255
1108	19	0.7138949605183171
1108	20	0.6974576415369214
1108	21	0.7405334904714707
1108	22	0.724082507309916
1108	23	0.7346853903257937
1108	24	0.7778589155690625
1108	25	0.9012915102170567
1109	1	0.39897679226426064
1109	2	0.3875941740563782
1109	3	0.4357095689518218
1109	4	0.4194073930527793
1109	5	0.01894684226897425
1109	6	0.28902611639193054
1109	7	0.30525229365760964
1109	8	0.38203866875697656
1109	9	0.5447746386144203
1109	10	0.1957844911541997
1109	11	0.47550913282838536
1109	12	0.6545212664664675
1109	13	0.44349309786550606
1109	14	0.7080727720281956
1109	15	0.6640586104187207
1109	16	0.5736714288511635
1109	17	0.6150648856165333
1109	18	0.8756064245601678
1109	19	0.823248955554682
1109	20	0.7707065815941405
1109	21	1.0516848640883794
1109	22	0.7596547902370787
1109	23	0.7651257836248888
1109	24	0.6767907862388114
1109	25	0.5607909242324283
1110	1	0.30635149383149596
1110	2	0.20226276744687866
1110	3	0.3209473835790355
1110	4	0.3029698678674322
1110	5	0.2942602237559626
1110	6	0.42689967189068057
1110	7	0.3627080216169105
1110	8	0.43353267410385143
1110	9	0.37624385941784055
1110	10	0.4386652177052923
1110	11	0.386153920924869
1110	12	0.5637974884701151
1110	13	0.4321652182871887
1110	14	0.5930887389936856
1110	15	0.4984122841206684
1110	16	0.5762959040211229
1110	17	0.5655025147929678
1110	18	0.5735684158162471
1110	19	0.6791183860734691
1110	20	0.5789682405120331
1110	21	0.6951990697047634
1110	22	0.6802567253379763
1110	23	0.6450570195156828
1110	24	0.6360906228861367
1110	25	0.5652378432796965
1111	1	0.15662119915166528
1111	2	0.06885645226221726
1111	3	0.04939607347476027
1111	4	0.136007690061966
1111	5	0.3314545305546801
1111	6	0.4819189403331734
1111	7	0.407090431242942
1111	8	0.42343337531124803
1111	9	0.42847200165446253
1111	10	0.36546981111163745
1111	11	0.3504370229458331
1111	12	0.5802620266928358
1111	13	0.5292007510047654
1111	14	0.5706735875559801
1111	15	0.5679018312219455
1111	16	0.45612347944035037
1111	17	0.7492292950639836
1111	18	0.49231335415987587
1111	19	0.25796279945888756
1111	20	0.5864786590421645
1111	21	0.6004131375624624
1111	22	0.22934520859914898
1111	23	0.2577668331586054
1111	24	0.3666036313980925
1111	25	0.4496320351639511
1112	1	-0.05229341558793266
1112	2	-0.39577765042399093
1112	3	-0.17260451880799366
1112	4	-0.06604295336754973
1112	5	-0.2106143586548157
1112	6	-0.26155125081361064
1112	7	-0.05225348001444985
1112	8	-0.06599828274417519
1112	9	-0.08986739371813712
1112	10	-0.3663402201621935
1112	11	-0.22333613276052944
1112	12	0.04112634729787601
1112	13	-0.026714754328494905
1112	14	-0.31779327878012253
1112	15	-0.48698065055125994
1112	16	-0.5224024741511499
1112	17	-0.208029802605175
1112	18	-0.07866609276084802
1112	19	-0.12317136777095072
1112	20	-0.1516952659809121
1112	21	-0.1492389234404925
1112	22	-0.14288562546730788
1112	23	0.03256227323227989
1112	24	0.049077160372760886
1112	25	-0.060456188931539145
1113	1	-0.5551989843337672
1113	2	-0.5346619146168585
1113	3	-0.37533917174524944
1113	4	-1.7962607171668665
1113	5	-0.6071433533394739
1113	6	-0.3720643545388853
1113	7	-0.3892681433599705
1113	8	-0.3128426025197726
1113	9	-0.16658861740190645
1113	10	-0.1878220591428743
1113	11	-0.5089233845790062
1113	12	0.014105885987149236
1113	13	-0.19556121837948837
1113	14	-0.17049969767825246
1113	15	-0.2143199459635241
1113	16	-0.5504097772119749
1113	17	-0.6462911904242716
1113	18	-0.34727556363444023
1113	19	-0.15234776519393733
1113	20	-0.015535073708726088
1113	21	-0.01033392909269537
1113	22	-0.05966312288413178
1113	23	-0.03909668980649883
1113	24	0.08024905114643843
1113	25	-0.10425360781131587
1114	1	-0.9545425591036363
1114	2	-2.209004874244382
1114	3	-1.6050791169018943
1114	4	-1.0828322964969885
1114	5	-1.0837556414229326
1114	6	-0.6710727450804512
1114	7	-0.8308897679372294
1114	8	-0.8783764503855025
1114	9	-1.2419339856534535
1114	10	-1.2300549985117115
1114	11	-1.3301069731280677
1114	12	-1.4124468016381044
1114	13	-1.3956301143220102
1114	14	-1.2564480694648168
1114	15	-1.3886150611865204
1114	16	-1.7568371945163435
1114	17	-1.8075733111069923
1114	18	-1.5791869612651865
1114	19	-1.837973802392277
1114	20	-1.5233425970362902
1114	21	-1.6524195076955905
1114	22	-1.3852483966498141
1114	23	-1.257520066309155
1114	24	-1.1491635015849369
1114	25	-1.6036999797017657
1115	1	-1.086741785396896
1115	2	-1.2584905900417027
1115	3	-0.6926754995811528
1115	4	-0.5098031187588035
1115	5	-0.764066620086254
1115	6	-0.8101429287286237
1115	7	-0.7493572958794275
1115	8	-0.9962743707179698
1115	9	-1.874882107530965
1115	10	-1.4773907637033048
1115	11	-1.9363844694998917
1115	12	-2.5655528483542263
1115	13	-2.5012216551769555
1115	14	-1.9856650767831923
1115	15	-2.4408664912487055
1115	16	-2.1165389954232268
1115	17	-1.9242450403442959
1115	18	-2.22771685460458
1115	19	-2.7574435824942753
1115	20	-2.859644831591119
1115	21	-2.2049730956899083
1115	22	-2.2712402180897584
1115	23	-2.2161652494328266
1115	24	-2.7252119782072626
1115	25	-2.601565490460416
1116	1	-1.4410463832149745
1116	2	-1.4930375655010941
1116	3	-1.5063704303095165
1116	4	-1.069607914535922
1116	5	-1.7024989013719072
1116	6	-2.761869451990158
1116	7	-2.1215149231958605
1116	8	-2.455803380195448
1116	9	-3.6953242495858163
1116	10	-2.3931065136897978
1116	11	-3.3856946763524753
1116	12	-5.562315580698865
1116	13	-4.108379003035548
1116	14	-3.637056741736867
1116	15	-4.598111920943776
1116	16	-4.1743236267388255
1116	17	-3.921218243026999
1116	18	-3.6994543129694506
1116	19	-4.1029692286557555
1116	20	-6.063769021251083
1116	21	-4.844481371500528
1116	22	-4.159451938153918
1116	23	-4.873824487198128
1116	24	-4.750158325810247
1116	25	-3.963888306590956
1201	1	0.6404442303632543
1201	2	0.7578108778730375
1201	3	0.8473573847277152
1201	4	0.7295694007387468
1201	5	0.12335621487285664
1201	6	0.7910494593854125
1201	7	0.954094857777271
1201	8	0.9573964409989841
1201	9	0.9337064316887762
1201	10	1.008026170341509
1201	11	0.7123399300377073
1201	12	1.134316690522418
1201	13	1.0778270477469292
1201	14	1.092045357288285
1201	15	1.0134312372929344
1201	16	1.0035080646827623
1201	17	0.7787065581363777
1201	18	1.2591467747395555
1201	19	1.4316071864716733
1201	20	1.2870702430391177
1201	21	0.9536514187494447
1201	22	0.9646691769978162
1201	23	1.0466632530034516
1201	24	0.9480617528015145
1201	25	1.1450709022068324
1202	1	0.7951250177577869
1202	2	0.8624574411910189
1202	3	0.7572635473709433
1202	4	0.6637756404782778
1202	5	0.7993257045877016
1202	6	0.8190382678912655
1202	7	0.8191008991989648
1202	8	0.7015239285095234
1202	9	1.1282237493663345
1202	10	1.0386487340565154
1202	11	0.9602942245573871
1202	12	0.9495523747607209
1202	13	0.947531590532428
1202	14	0.928969475164557
1202	15	0.9701108778246592
1202	16	1.1069323395524648
1202	17	1.015709393845583
1202	18	0.9610059307487688
1202	19	1.1694333163698545
1202	20	0.9152476402108789
1202	21	1.0425632601816588
1202	22	1.0795647825084558
1202	23	1.3052535453551926
1202	24	1.248922587829675
1202	25	1.3954081064068902
1203	1	0.7915588054187808
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2211	10	-0.16915741953458507
2211	11	-0.12791826875474754
2211	12	-0.12292196643729625
2211	13	-0.04251808807318014
2211	14	-0.09615853625945192
2211	15	-0.21279050722315418
2211	16	-0.16948897345478287
2211	17	-0.39210636766696894
2211	18	-0.2746683757853745
2211	19	-0.2515509492458392
2211	20	-0.43349541759489796
2211	21	-0.25498747341140415
2211	22	-0.40365903307974094
2211	23	-0.4143369122832965
2211	24	-0.3175609317591821
2211	25	-0.6234535621311252
2212	1	0.03803843698116083
2212	2	0.10857239439148003
2212	3	0.07529765915849751
2212	4	0.11860180496692863
2212	5	-0.1518188589568581
2212	6	0.16956638816341751
2212	7	-0.004169205120049924
2212	8	0.20123495616045517
2212	9	0.11478839947208996
2212	10	0.010956327410902134
2212	11	0.2181372603703764
2212	12	0.4521736336174911
2212	13	0.5480357074562363
2212	14	0.6141611337446591
2212	15	0.3516079783133961
2212	16	0.25856545280072396
2212	17	0.2973502347737593
2212	18	0.12851201548105706
2212	19	0.36703726951159155
2212	20	0.304315350160941
2212	21	0.3369696876724362
2212	22	0.41556225446648654
2212	23	0.12277636251865687
2212	24	-0.011742123146710526
2212	25	0.1875628799970528
2213	1	-0.34813438649127093
2213	2	-0.023435690220967587
2213	3	-0.11618157058465073
2213	4	-0.33322467349169216
2213	5	-0.01533961568523523
2213	6	-0.009558644643114178
2213	7	-0.47975843754183956
2213	8	-0.45854842601065116
2213	9	-0.20886260934576484
2213	10	-0.10410058685378942
2213	11	-0.02940935196595973
2213	12	-0.3177666517249591
2213	13	-0.1876423799430782
2213	14	-0.16676967896452766
2213	15	-0.41151395917967193
2213	16	-1.027682047814821
2213	17	-0.6693169730316271
2213	18	-0.18696915153870464
2213	19	-0.23401903326634255
2213	20	-0.1119569616648306
2213	21	-0.3072478392695075
2213	22	-0.4494222824946448
2213	23	-0.3451611684277154
2213	24	-0.22064872530963697
2213	25	-0.22077633821984222
2214	1	-1.21424051022192
2214	2	-0.6158944345225521
2214	3	-0.7251519397504893
2214	4	-0.801351387222283
2214	5	-1.200288411704804
2214	6	-1.7019375783966204
2214	7	-1.1741260931490416
2214	8	-1.0195207876365444
2214	9	-0.9097889350513029
2214	10	-1.0424674985593967
2214	11	-1.3058930898658758
2214	12	-1.009968955576987
2214	13	-1.4016897840442581
2214	14	-0.9304799909733035
2214	15	-1.1082404183389158
2214	16	-1.0586591008804263
2214	17	-1.135968499992451
2214	18	-1.1999635984441284
2214	19	-1.2459209915218779
2214	20	-1.3836716922247945
2214	21	-1.5599108387629812
2214	22	-1.974859791956959
2214	23	-1.9435912183139763
2214	24	-1.7181048809084345
2214	25	-1.9916733377544347
2215	1	-1.480653518998281
2215	2	-0.9154476580570723
2215	3	-0.9827140638524057
2215	4	-1.0125468822875874
2215	5	-1.5009233844759144
2215	6	-1.624553060820439
2215	7	-1.8754285164697428
2215	8	-1.6047853067216096
2215	9	-1.5486944615179326
2215	10	-1.6475540430985731
2215	11	-1.2498288997908276
2215	12	-1.5196902079127597
2215	13	-1.662029540249307
2215	14	-1.856466077971426
2215	15	-1.6900039157080329
2215	16	-1.712567198887868
2215	17	-1.6669419961010945
2215	18	-1.5452154944379544
2215	19	-1.8079841400998902
2215	20	-1.5567552502804318
2215	21	-1.5057092906862124
2215	22	-1.557472955853811
2215	23	-1.9941380962610182
2215	24	-1.8880731483707578
2215	25	-2.1919512849748273
2216	1	-1.5152282668778376
2216	2	-1.0077663055872073
2216	3	-0.9895418776476603
2216	4	-1.2394053828357912
2216	5	-2.0824526714921205
2216	6	-2.2707331322726816
2216	7	-2.1742578377951
2216	8	-2.1766834768026335
2216	9	-4.089062454752522
2216	10	-2.1494973647424658
2216	11	-2.3213780086202185
2216	12	-2.7118158186009964
2216	13	-2.6958511988973655
2216	14	-2.956421496350451
2216	15	-2.758823611097739
2216	16	-2.175839399757919
2216	17	-2.322703173776766
2216	18	-2.859052760032455
2216	19	-3.0010941958798583
2216	20	-2.33581603559729
2216	21	-2.8692421104410712
2216	22	-2.4744434601217336
2216	23	-3.4546612848989433
2216	24	-2.8210569924328546
2216	25	-3.039875468735808
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41470.0
3	2.0
4	717.0
5	5898.0
6	7385.0
7	5722.0
8	4511.0
9	3775.0
10	3117.0
11	2880.0
12	2526.0
13	2371.0
14	2275.0
15	2299.0
16	2355.0
17	2460.0
18	2811.0
19	3383.0
20	4438.0
21	6056.0
22	7381.0
23	8795.0
24	10209.0
25	11655.0
26	13012.0
27	14042.0
28	15928.0
29	18794.0
30	23739.0
31	30400.0
32	37833.0
33	48802.0
34	63230.0
35	82859.0
36	113551.0
37	175490.0
38	552469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.80649358343189	19.287724568635493	13.83950968165753	26.06627216627509
2	13.857275318777972	20.583788516272275	39.62411521356681	25.934820951382935
3	17.494501212168903	25.4254956403137	29.524687402811033	27.55531574470636
4	11.845212939734264	16.752388060148842	36.40858832450643	34.99381067561046
5	13.170104039190619	38.63666169314921	34.09364951015475	14.099584757505426
6	32.226361454707394	35.93712631639713	17.86311643971694	13.973395789178536
7	28.546652220043566	31.85855306322147	21.05912742896348	18.535667287771485
8	26.385817291473312	35.52529188713447	20.220990084758597	17.867900736633615
9	27.33505952358648	14.324406356676873	19.69213359363583	38.64840052610082
10	16.444778276526904	28.696315753176155	32.297729242191174	22.561176728105764
11	34.56122958969967	22.516426774687368	23.68924865817098	19.233094977441986
12	24.05367739615177	24.92207636516214	30.290340466342986	20.733905772343103
13	29.405946529193145	21.60914458697998	25.77246433281381	23.212444551013068
14	21.754770589134413	22.125508378314386	27.17557446374892	28.94414656880228
15	23.987281680209044	30.193869697921173	23.102124990539195	22.71672363133059
16	22.57107060376855	27.592059455174077	26.91226345392119	22.924606487136177
17	21.909090704736986	28.11814626741087	27.10786815057587	22.864894877276274
18	21.819401150941133	26.360223594293252	29.731297206569955	22.08907804819566
19	23.677944233066043	26.199324754650366	27.891034641914665	22.23169637036893
20	24.455636867962955	25.758916496145385	28.119190818749423	21.66625581714224
21	23.952769250711242	25.691141949246198	27.133854173300797	23.222234626741763
22	24.229922848051046	26.810995332820337	27.04341773318478	21.91566408594384
23	23.080606006114007	26.575780734879817	27.837993766109214	22.50561949289696
24	23.71815620691722	25.863903374692804	28.0931936702032	22.324746748186776
25	23.04149433555116	26.327249295690226	28.18999880117485	22.441257567583765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	102.0
2	314.5
3	527.0
4	527.0
5	527.0
6	1256.0
7	1985.0
8	1985.0
9	1985.0
10	2216.0
11	2447.0
12	2447.0
13	2447.0
14	3293.5
15	4140.0
16	4140.0
17	4140.0
18	8184.0
19	12228.0
20	12228.0
21	12228.0
22	20221.5
23	28215.0
24	28215.0
25	28215.0
26	43760.5
27	59306.0
28	59306.0
29	59306.0
30	75447.0
31	91588.0
32	91588.0
33	91588.0
34	103439.5
35	115291.0
36	115291.0
37	115291.0
38	124785.0
39	134279.0
40	134279.0
41	134279.0
42	144927.0
43	155575.0
44	155575.0
45	155575.0
46	168867.5
47	182160.0
48	182160.0
49	182160.0
50	177411.0
51	172662.0
52	172662.0
53	172662.0
54	154261.0
55	135860.0
56	135860.0
57	135860.0
58	118000.0
59	100140.0
60	100140.0
61	100140.0
62	85098.0
63	70056.0
64	70056.0
65	70056.0
66	53662.0
67	37268.0
68	37268.0
69	37268.0
70	27949.5
71	18631.0
72	18631.0
73	18631.0
74	13335.5
75	8040.0
76	8040.0
77	8040.0
78	5448.0
79	2856.0
80	2856.0
81	2856.0
82	1884.5
83	913.0
84	913.0
85	913.0
86	620.5
87	328.0
88	328.0
89	328.0
90	183.0
91	38.0
92	38.0
93	38.0
94	21.5
95	5.0
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0853413654618474
2	0.042033806869268116
3	0.019106275849667325
4	0.006293832044596295
5	0.01790745069831565
6	0.05926691841994845
7	0.0745519390996823
8	0.054996103818258105
9	0.022103338728046516
10	0.0476532997662291
11	0.006069052328717857
12	0.0
13	0.005994125756758377
14	2.9970628783791887E-4
15	0.014011268956422705
16	0.009141041779056523
17	0.004270814601690344
18	0.005994125756758377
19	0.001048972007432716
20	5.24486003716358E-4
21	7.492657195947972E-5
22	7.492657195947972E-5
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1334640.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.791556885760905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.15293056337191	41.725376269803924
2	13.558607321040157	14.85794408644932
3	4.548224343722548	7.476128785748296
4	1.977862908762371	4.3348075231075995
5	1.0836553709065868	2.9687582449794294
6	0.6537608112192059	2.1492343606558992
7	0.4183798668438321	1.6046578991821627
8	0.286541616940731	1.2560049043796777
9	0.19825012605406414	0.9776189753370471
>10	0.9966266250061959	9.71001962302875
>50	0.0707390229404144	2.6506464822111284
>100	0.04660779946430117	5.023970879252395
>500	0.00507200136706157	1.964472724080981
>1k	0.0024674601245164396	2.3530860628076105
>5k	2.7416223605738213E-4	0.9472731789756961
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7336	0.5496613318947432	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5276	0.395312593658215	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4305	0.3225588922855602	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2765	0.20717197146796143	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2521	0.18888988790984834	No Hit
GTCCTACAGTGGACATTTCTAAATT	2129	0.1595186717017323	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1983	0.14857939219564828	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1943	0.14558232931726908	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1812	0.13576694839057724	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1643	0.12310435772942517	No Hit
GGATACCACGTGTCCCGCCCTACTC	1580	0.11838398369597794	No Hit
ATCATTAACTGAATCCATAGGTTAA	1481	0.11096625307198944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGCCG	70	4.390804E-7	14.941663	5
CGAGTAA	40	0.005287297	14.246458	10
GGTATCA	1320	0.0	14.047451	1
TAGGACC	390	0.0	12.6777725	4
TCCAACG	115	5.3732947E-9	12.383119	18
GTATTAC	110	3.7614882E-8	12.10242	1
GTATAAA	95	1.0275162E-6	12.011423	1
CTACACG	65	7.96334E-4	11.70256	4
GGCCGTG	95	1.350463E-5	11.006754	7
TTAGACA	105	3.4282093E-6	10.866664	4
GTATCAA	2950	0.0	10.830639	1
TAGAAAT	345	0.0	10.748548	4
TGGCGAG	1220	0.0	10.583169	18
GTATTAG	90	9.420507E-5	10.565604	1
GTATAAT	190	9.094947E-12	10.509995	1
CTCCTAA	100	2.3726225E-5	10.459949	1
GAACAGT	275	0.0	10.372724	6
CCAGAAC	330	0.0	10.3723345	3
GTCCTAC	1080	0.0	10.301464	1
GTCCTAG	195	1.4551915E-11	10.240508	1
>>END_MODULE