FastQCFastQC Report
Thu 26 May 2016
SRR1512906_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512906_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481838
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT99330.6703161884092593No Hit
GTCCTACAGTGGACATTTCTAAATT70140.47333109287250025No Hit
GTCCTAAAGTGTGTATTTCTCATTT64930.43817205389523006No Hit
CTGTAGGACGTGGAATATGGCAAGA59880.40409275507849035No Hit
CTTTAGGACGTGAAATATGGCGAGG58210.39282296715295467No Hit
TATCAACGCAGAGTACTTTTTTTTT57970.3912033569121591No Hit
GGTATCAACGCAGAGTACTTTTTTT53620.3618479212977397No Hit
CTGAAGGACCTGGAATATGGCGAGA29830.20130405617888053No Hit
GTCCTACAGTGTGCATTTCTCATTT29690.20035928353841648No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27920.18841465801254928No Hit
GTCCTTCAGTGTGCATTTCTCATTT25110.1694517214432347No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22720.1533231027953123No Hit
ATTTAGAAATGTCCACTGTAGGACG22570.1523108463948151No Hit
TTTCTAAATTTTCCACCTTTTTCAG22370.15096117119415212No Hit
CTGTAGGACCTGGAATATGGCGAGA22110.1492065934332903No Hit
GAATATGGCAAGAAAACTGAAAATC20970.1415134447895114No Hit
GAGTACTTTTTTTTTTTTTTTTTTT18730.12639708254208623No Hit
GGAATATGGCGAGAAAACTGAAAAT17400.11742174245767756No Hit
ATTCCAGGTCCTTCAGTGTGCATTT17250.11640948605718034No Hit
GTACTGGTTCACTATCGGTCAGTCA15250.10291273405055074No Hit
GCCATATTCCACGTCCTACAGTGGA14960.10095570500958943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT250.00603746118.9969946
TAGGACC7500.014.3110684
CCTTTCG400.00528330714.24822714
CTACGCT400.005284490514.2477454
TGGCGAC551.9621232E-413.816462518
GGTATCA16850.013.0596141
CGGTTTC905.4125303E-712.66509113
CTGGTCG604.098455E-412.6642359
AATACGG1001.4445686E-712.34846314
GTAATAC1001.4452053E-712.3480463
TCCAACG1700.012.29258718
GCGCCAC550.003071188512.08940413
GGCGACA550.003071965612.08899619
CACCTTT7600.011.74853814
GCCGGTT1157.090239E-811.56299711
GTCCTAG2600.011.3581251
GGCGAGG17050.011.19764119
GGACCTG18450.011.1716746
TTCCACC8000.011.16035711
CCACCTT8100.011.14058913
Produced by FastQC (version 0.11.5)