FastQCFastQC Report
Thu 26 May 2016
SRR1512906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481838
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125220.8450316431350795No Hit
TATCAACGCAGAGTACTTTTTTTTT93170.6287461922288401No Hit
GGTATCAACGCAGAGTACTTTTTTT71640.4834536568774725No Hit
GTCCTACAGTGGACATTTCTAAATT64750.4369573462146334No Hit
CTTTAGGACGTGAAATATGGCGAGG58860.3972094115551093No Hit
GTCCTAAAGTGTGTATTTCTCATTT57850.39039355179176133No Hit
CTGTAGGACGTGGAATATGGCAAGA56790.3832402732282476No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45700.30840078335148646No Hit
CTGAAGGACCTGGAATATGGCGAGA31350.21156158770391903No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28640.19327348873493594No Hit
GTCCTACAGTGTGCATTTCTCATTT28010.18902201185284762No Hit
TTTCTAAATTTTCCACCTTTTTCAG23960.16169108903942267No Hit
ATTTAGAAATGTCCACTGTAGGACG23320.1573721283973012No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22850.15420039167574323No Hit
CTGTAGGACCTGGAATATGGCGAGA21550.145427502871434No Hit
GTCCTTCAGTGTGCATTTCTCATTT21400.1444152464709368No Hit
GAATATGGCAAGAAAACTGAAAATC21260.1434704738304727No Hit
GGAATATGGCGAGAAAACTGAAAAT16910.11411503821605332No Hit
ATTCCAGGTCCTTCAGTGTGCATTT16740.1129678142954898No Hit
GCCATATTCCACGTCCTACAGTGGA15990.10790653229300368No Hit
TTCCAGGTCCTTCAGTGTGCATTTC15730.10615195453214184No Hit
GCAGAGTACTTTTTTTTTTTTTTTT15660.10567956821190981No Hit
ACAGTGGACATTTCTAAATTTTCCA15080.10176551012998722No Hit
ACCTGGAATATGGCGAGAAAACTGA15050.10156305884988777No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG307.764314E-418.98474919
GGTATCA16950.014.1411861
GCGTGCG759.689484E-713.931089
CGTGCGC759.700543E-713.92966810
GCCGGTT1301.8189894E-1213.87627911
GGCGTGC1205.0931703E-1113.4579748
TAGGACC7600.013.2626354
CTAGGAC2500.012.1732433
AATGTCC8200.012.16430958
GTGCGCC951.0472195E-611.99279511
GCGCCAC1052.741799E-711.75444713
CGCCACC1052.741799E-711.75444714
GGCGAGG14000.011.72986219
GTCGAGA658.077264E-411.68292119
CCGGTTT1552.5465852E-1111.63777612
TGCCGGT1657.2759576E-1211.51212210
TCCAACG2250.011.39084818
TAGAAAT8900.011.3253964
TGTCGAG1850.011.28822918
CCTATAC1354.645699E-911.271523
Produced by FastQC (version 0.11.5)