##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512906_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1481838 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02813870342102 31.0 30.0 34.0 26.0 34.0 2 30.063452280208768 31.0 30.0 34.0 26.0 34.0 3 30.215650428724327 31.0 30.0 34.0 26.0 34.0 4 33.74374729221413 37.0 35.0 37.0 30.0 37.0 5 33.343694115011225 37.0 35.0 37.0 27.0 37.0 6 33.1968622750935 37.0 35.0 37.0 26.0 37.0 7 33.00329860619042 37.0 35.0 37.0 26.0 37.0 8 32.93273758669976 37.0 35.0 37.0 26.0 37.0 9 34.1997148136301 39.0 35.0 39.0 25.0 39.0 10 34.10401406901429 39.0 35.0 39.0 25.0 39.0 11 34.07556561513472 39.0 35.0 39.0 24.0 39.0 12 33.961626709532354 39.0 35.0 39.0 23.0 39.0 13 33.935500371835516 39.0 35.0 39.0 22.0 39.0 14 34.984949096999806 39.0 35.0 41.0 22.0 41.0 15 35.00616194212863 40.0 35.0 41.0 23.0 41.0 16 35.018066752236074 40.0 35.0 41.0 23.0 41.0 17 35.04862542329189 40.0 35.0 41.0 24.0 41.0 18 34.98186576400389 40.0 35.0 41.0 22.0 41.0 19 35.00072949944596 40.0 35.0 41.0 22.0 41.0 20 35.00003374188002 40.0 35.0 41.0 22.0 41.0 21 34.961367571893824 40.0 35.0 41.0 22.0 41.0 22 34.92204208557211 40.0 35.0 41.0 22.0 41.0 23 34.77111465625797 40.0 35.0 41.0 19.0 41.0 24 34.74538647274533 40.0 35.0 41.0 19.0 41.0 25 34.67797222098502 40.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8610586548274846 1101 2 0.7656913103984948 1101 3 0.7561090357727451 1101 4 0.7735481233481849 1101 5 0.8535861502272866 1101 6 0.8999275183715056 1101 7 0.95632190118409 1101 8 0.6788243817652031 1101 9 1.0039302827867687 1101 10 0.6720089886152465 1101 11 1.024738694606171 1101 12 1.1407538591669422 1101 13 1.1513277956988262 1101 14 1.2199093812053832 1101 15 0.9342180092100776 1101 16 1.0235410873340882 1101 17 1.2105847134148533 1101 18 1.2326835448440576 1101 19 1.079182794668185 1101 20 1.1371389780302863 1101 21 1.1254912674303554 1101 22 1.1254516644428918 1101 23 1.2558374494894196 1101 24 1.368405537037411 1101 25 1.370138235472993 1102 1 1.1665165675005902 1102 2 1.2230287781579783 1102 3 1.029033503156839 1102 4 1.039458281121938 1102 5 1.3396589081936554 1102 6 1.1754227789880147 1102 7 1.148800264868136 1102 8 1.1651985164778509 1102 9 1.263406111306523 1102 10 1.3186356627162397 1102 11 1.4402626640666654 1102 12 1.3859628688073258 1102 13 1.559143242202893 1102 14 1.4712878905640352 1102 15 1.495899232318493 1102 16 1.598270031916762 1102 17 1.622195009223205 1102 18 1.180841234854661 1102 19 1.452719161530183 1102 20 1.5675639247521644 1102 21 1.5068639806585367 1102 22 1.3334322896462893 1102 23 1.5116675560162776 1102 24 1.4501880932558393 1102 25 1.4355347855632417 1103 1 0.7758743386202731 1103 2 0.8838049688906295 1103 3 0.6875632959213114 1103 4 0.4702798533454029 1103 5 0.6439700080924666 1103 6 0.6414121720212052 1103 7 0.8067985754634819 1103 8 0.7848911355369452 1103 9 0.7763381354457337 1103 10 0.8646584753482216 1103 11 0.9864300398486492 1103 12 1.1042029116660501 1103 13 1.0650320646368243 1103 14 1.2506214544425234 1103 15 1.2241849968166179 1103 16 1.133033926277946 1103 17 1.042792281798441 1103 18 0.9714280478608046 1103 19 1.164893221231928 1103 20 1.0609614989258134 1103 21 1.0220338990512232 1103 22 1.0218136642434317 1103 23 1.167989217528742 1103 24 1.1874594496266795 1103 25 1.0367261559935557 1104 1 0.9936920022501958 1104 2 1.1647606642937802 1104 3 1.0642175794861082 1104 4 0.8916272501483107 1104 5 1.0603600455987134 1104 6 1.022503504948176 1104 7 1.0389384907861299 1104 8 1.0481864958550062 1104 9 1.081348212160563 1104 10 1.2678087529383788 1104 11 1.1923766744455548 1104 12 1.196174213621532 1104 13 1.0690730469380512 1104 14 1.3431007106876152 1104 15 1.364952172535851 1104 16 1.3238380316468863 1104 17 1.2593414325537822 1104 18 1.0626140799415538 1104 19 1.4374628658835817 1104 20 1.399642767219035 1104 21 1.4486338177785356 1104 22 1.371518913470517 1104 23 1.3801596650227452 1104 24 1.5927095004221101 1104 25 1.389212798168586 1105 1 0.6525344010213878 1105 2 0.6635517478638668 1105 3 0.7198266882889222 1105 4 0.5905520085676699 1105 5 0.5549574944962856 1105 6 0.7127353453651253 1105 7 0.7708300743366223 1105 8 0.756256059463027 1105 9 1.031565982607276 1105 10 0.8168937320461964 1105 11 1.0418408267551769 1105 12 1.03125160183518 1105 13 0.9804847519644682 1105 14 1.1349566768818633 1105 15 1.1441720153142327 1105 16 1.0139356230679226 1105 17 0.8331256939107377 1105 18 0.7814436583557978 1105 19 1.1319141775722628 1105 20 1.1214725996087438 1105 21 0.968821658298161 1105 22 0.9780915078973749 1105 23 1.1853397174119351 1105 24 1.086942028464108 1105 25 1.0897076788068745 1106 1 0.04582683793722708 1106 2 0.3353397684262305 1106 3 -1.710502331297711 1106 4 -0.41589813620205973 1106 5 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0.9367528252877264 1107 18 1.0581165974924573 1107 19 1.0017673383171015 1107 20 1.0416503924870142 1107 21 1.0475629426248148 1107 22 1.107583612996585 1107 23 1.1904757945363826 1107 24 1.1700151013899571 1107 25 1.1791406958836745 1108 1 0.5171504148667019 1108 2 0.5205701051727232 1108 3 0.573323050293773 1108 4 0.43286121882205464 1108 5 0.6036835961948128 1108 6 0.4960498009458547 1108 7 0.5082800910699348 1108 8 0.4409956464769138 1108 9 0.6316701824683406 1108 10 0.5061138130860883 1108 11 0.5919504266090456 1108 12 0.7121181940130583 1108 13 0.7247502596104596 1108 14 0.8838630116734123 1108 15 0.9075608307958163 1108 16 0.6899247217491222 1108 17 0.7978327409680119 1108 18 0.7041197614543293 1108 19 0.8604318728820033 1108 20 0.8162973398424214 1108 21 0.8664977947299803 1108 22 0.8172462223577526 1108 23 0.9718096687853333 1108 24 0.9974138145017264 1108 25 0.8986609335358722 1109 1 0.6022044388597116 1109 2 0.4807572029720397 1109 3 0.5726153095030106 1109 4 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2215 15 -2.136268427515901 2215 16 -2.1643191403289066 2215 17 -2.374082466920072 2215 18 -1.952913813975762 2215 19 -2.249940950151391 2215 20 -2.166407527631634 2215 21 -1.9152985004876797 2215 22 -2.0992746411256604 2215 23 -2.71415566403423 2215 24 -2.5540519088687503 2215 25 -2.6826539311453352 2216 1 -3.0954661910257784 2216 2 -2.3522902459995585 2216 3 -2.3476472341628885 2216 4 -2.670122783236529 2216 5 -3.6955625842365265 2216 6 -3.870575181533191 2216 7 -3.579006179403436 2216 8 -3.806922497703308 2216 9 -5.905509288618418 2216 10 -4.146938285349265 2216 11 -3.9027415078929124 2216 12 -4.2163447839429615 2216 13 -4.271758362792514 2216 14 -4.33350668993684 2216 15 -4.340748527591963 2216 16 -3.7906735640213505 2216 17 -3.898605431496783 2216 18 -4.184564106085141 2216 19 -4.253005602928617 2216 20 -3.727830890551367 2216 21 -4.465674448308189 2216 22 -3.9570443619321694 2216 23 -5.519328911002486 2216 24 -4.594949872451593 2216 25 -4.8521047584425645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49381.0 3 1.0 4 815.0 5 6883.0 6 9088.0 7 6886.0 8 5516.0 9 4670.0 10 3884.0 11 3256.0 12 2888.0 13 2545.0 14 2546.0 15 2528.0 16 2628.0 17 2714.0 18 3050.0 19 3835.0 20 5292.0 21 6945.0 22 8525.0 23 10095.0 24 11451.0 25 13013.0 26 14272.0 27 15261.0 28 16869.0 29 20391.0 30 25029.0 31 31476.0 32 40266.0 33 51980.0 34 67693.0 35 88975.0 36 122414.0 37 193536.0 38 625241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.179472424394696 20.712705256844597 14.221888119822989 25.885934198937715 2 12.25481790330573 21.435399656775445 42.89523819811159 23.414544241807235 3 16.87900303266954 27.00277347855228 30.566717042414847 25.551506446363327 4 10.802504337779204 18.094630062176524 38.767159620151595 32.33570597989268 5 11.143434332525853 40.40685411285244 35.885952875443024 12.563758679178678 6 29.517702126740097 38.82887493990352 18.721808674420238 12.931614258936145 7 26.373716915342584 33.22867626252018 22.814340967739103 17.58326585439813 8 24.01983855012475 39.29599481415708 20.656400768423076 16.02776586729509 9 26.585042664944 14.857026367144652 21.299260780315198 37.25867018759615 10 15.471100896370496 30.119831150697735 34.02372856637436 20.385339386557412 11 32.105220178291475 22.910771253351655 26.809724572138936 18.174283996217937 12 24.14789540777592 25.700704061394315 32.253340630611724 17.89805990021804 13 28.900516210800813 23.700682769600185 26.561471872281523 20.83732914731748 14 19.560514015853457 24.92715049445885 28.552957102809916 26.959378386877773 15 21.183467575925693 35.10926361627541 23.78881325228833 19.918455555510562 16 19.259428731859778 29.134094745232066 31.17536972210018 20.43110680080798 17 19.174054237910386 30.959772380021704 29.794137813746822 20.072035568321088 18 18.986453335780862 27.292077634337925 34.66746121353762 19.054007816343596 19 22.377234929620045 26.893118839476482 30.087109448284334 20.642536782619143 20 22.915478652868273 27.796452323670636 30.882802509085145 18.405266514375953 21 20.904489718793652 26.98453938710918 29.68505159161307 22.42591930248409 22 22.1400488316442 29.936038374276063 29.428151483372545 18.495761310707195 23 19.37222557391564 29.662081820010016 31.413622811670372 19.552069794403977 24 22.2514201957299 26.98068209885291 31.85577640740756 18.912121298009634 25 20.50075649294997 28.576335604836693 31.49588551515078 19.427022387062554 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 182.0 2 581.0 3 980.0 4 980.0 5 980.0 6 2406.5 7 3833.0 8 3833.0 9 3833.0 10 4593.5 11 5354.0 12 5354.0 13 5354.0 14 6959.0 15 8564.0 16 8564.0 17 8564.0 18 15421.5 19 22279.0 20 22279.0 21 22279.0 22 37024.5 23 51770.0 24 51770.0 25 51770.0 26 76710.5 27 101651.0 28 101651.0 29 101651.0 30 128387.0 31 155123.0 32 155123.0 33 155123.0 34 165728.0 35 176333.0 36 176333.0 37 176333.0 38 177649.5 39 178966.0 40 178966.0 41 178966.0 42 182023.0 43 185080.0 44 185080.0 45 185080.0 46 187805.0 47 190530.0 48 190530.0 49 190530.0 50 173049.0 51 155568.0 52 155568.0 53 155568.0 54 129157.5 55 102747.0 56 102747.0 57 102747.0 58 84817.0 59 66887.0 60 66887.0 61 66887.0 62 54070.5 63 41254.0 64 41254.0 65 41254.0 66 30731.0 67 20208.0 68 20208.0 69 20208.0 70 14689.0 71 9170.0 72 9170.0 73 9170.0 74 6422.0 75 3674.0 76 3674.0 77 3674.0 78 2444.0 79 1214.0 80 1214.0 81 1214.0 82 773.0 83 332.0 84 332.0 85 332.0 86 225.0 87 118.0 88 118.0 89 118.0 90 66.5 91 15.0 92 15.0 93 15.0 94 10.5 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08462463508156763 2 0.039950385939623634 3 0.020582546810110146 4 0.006006054642950174 5 0.018558034009115706 6 0.05979061138936915 7 0.07618916507742411 8 0.059588160109269706 9 0.027735825373623835 10 0.05088275506499361 11 0.007220762323546838 12 4.7238632023203613E-4 13 0.005331217042618693 14 0.0010797401605303683 15 0.01295688192636442 16 0.007895599923878317 17 0.004723863202320361 18 0.0047913469623535095 19 0.0016196102407955524 20 0.0015521264807624047 21 5.398700802651842E-4 22 8.098051203977762E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1481838.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.991538686539535 #Duplication Level Percentage of deduplicated Percentage of total 1 80.96169156529416 47.76054760101731 2 12.009523175645377 14.169205020459547 3 3.4832299382941248 6.164432809769717 4 1.2929262312313787 3.0508683115411057 5 0.6550959943750656 1.9322560347786881 6 0.36897927742625647 1.3059993191293446 7 0.2275657019969648 0.9397115639158432 8 0.16688016870132485 0.7875614342368359 9 0.1136519843682846 0.6034054889397281 >10 0.6166176691986452 6.700327093161304 >50 0.053388211209556405 2.183072493862777 >100 0.04114159541435797 5.19661703591264 >500 0.00494154239898989 1.9054532074699013 >1k 0.0035625073108996874 3.7191827094676144 >5k 6.895175440451009E-4 2.732457999084991 >10k+ 1.1491959067418348E-4 0.8489018772525743 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12522 0.8450316431350795 No Hit TATCAACGCAGAGTACTTTTTTTTT 9317 0.6287461922288401 No Hit GGTATCAACGCAGAGTACTTTTTTT 7164 0.4834536568774725 No Hit GTCCTACAGTGGACATTTCTAAATT 6475 0.4369573462146334 No Hit CTTTAGGACGTGAAATATGGCGAGG 5886 0.3972094115551093 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5785 0.39039355179176133 No Hit CTGTAGGACGTGGAATATGGCAAGA 5679 0.3832402732282476 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4570 0.30840078335148646 No Hit CTGAAGGACCTGGAATATGGCGAGA 3135 0.21156158770391903 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2864 0.19327348873493594 No Hit GTCCTACAGTGTGCATTTCTCATTT 2801 0.18902201185284762 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2396 0.16169108903942267 No Hit ATTTAGAAATGTCCACTGTAGGACG 2332 0.1573721283973012 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2285 0.15420039167574323 No Hit CTGTAGGACCTGGAATATGGCGAGA 2155 0.145427502871434 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2140 0.1444152464709368 No Hit GAATATGGCAAGAAAACTGAAAATC 2126 0.1434704738304727 No Hit GGAATATGGCGAGAAAACTGAAAAT 1691 0.11411503821605332 No Hit ATTCCAGGTCCTTCAGTGTGCATTT 1674 0.1129678142954898 No Hit GCCATATTCCACGTCCTACAGTGGA 1599 0.10790653229300368 No Hit TTCCAGGTCCTTCAGTGTGCATTTC 1573 0.10615195453214184 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1566 0.10567956821190981 No Hit ACAGTGGACATTTCTAAATTTTCCA 1508 0.10176551012998722 No Hit ACCTGGAATATGGCGAGAAAACTGA 1505 0.10156305884988777 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.748376003314802E-5 2 0.0 0.0 0.0 0.0 6.748376003314802E-5 3 0.0 0.0 0.0 0.0 6.748376003314802E-5 4 0.0 0.0 0.0 0.0 6.748376003314802E-5 5 0.0 0.0 0.0 0.0 6.748376003314802E-5 6 0.0 0.0 0.0 0.0 6.748376003314802E-5 7 0.0 0.0 0.0 0.0 6.748376003314802E-5 8 0.0 0.0 0.0 0.0 6.748376003314802E-5 9 0.0 0.0 0.0 0.0 1.3496752006629604E-4 10 0.0 0.0 0.0 0.0 1.3496752006629604E-4 11 0.0 0.0 0.0 0.0 1.3496752006629604E-4 12 0.0 0.0 0.0 0.0 2.699350401325921E-4 13 0.0 0.0 0.0 0.0 2.699350401325921E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 30 7.764314E-4 18.984749 19 GGTATCA 1695 0.0 14.141186 1 GCGTGCG 75 9.689484E-7 13.93108 9 CGTGCGC 75 9.700543E-7 13.929668 10 GCCGGTT 130 1.8189894E-12 13.876279 11 GGCGTGC 120 5.0931703E-11 13.457974 8 TAGGACC 760 0.0 13.262635 4 CTAGGAC 250 0.0 12.173243 3 AATGTCC 820 0.0 12.1643095 8 GTGCGCC 95 1.0472195E-6 11.992795 11 GCGCCAC 105 2.741799E-7 11.754447 13 CGCCACC 105 2.741799E-7 11.754447 14 GGCGAGG 1400 0.0 11.729862 19 GTCGAGA 65 8.077264E-4 11.682921 19 CCGGTTT 155 2.5465852E-11 11.637776 12 TGCCGGT 165 7.2759576E-12 11.512122 10 TCCAACG 225 0.0 11.390848 18 TAGAAAT 890 0.0 11.325396 4 TGTCGAG 185 0.0 11.288229 18 CCTATAC 135 4.645699E-9 11.27152 3 >>END_MODULE