FastQCFastQC Report
Thu 26 May 2016
SRR1512905_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512905_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915137
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65280.3408633429357795No Hit
GTCCTAAAGTGTGTATTTCTCATTT56550.29527913668839356No Hit
GTATCAACGCAGAGTACTTTTTTTT53630.28003218568697696No Hit
CTGTAGGACGTGGAATATGGCAAGA52050.27178212315881317No Hit
CTTTAGGACGTGAAATATGGCGAGG45970.240035047101069No Hit
GTCCTACAGTGTGCATTTCTCATTT35320.1844254484143954No Hit
TATCAACGCAGAGTACTTTTTTTTT30700.16030184785735957No Hit
GGTATCAACGCAGAGTACTTTTTTT29830.15575909190830736No Hit
CTGTAGGACCTGGAATATGGCGAGA25800.13471621090292757No Hit
GTACTGGTTCACTATCGGTCAGTCA24200.1263617172035212No Hit
CTGAAGGACCTGGAATATGGCGAGA22380.11685848062044649No Hit
GTCCTTCAGTGTGCATTTCTCATTT19250.10051500232098277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1006.6211214E-1014.24792319
TCCAACG1600.013.65425918
TAGGACC10700.013.2270434
TATTGCG550.00307224412.08914716
CTAATAC1459.822543E-1111.7913843
GGCGAGG11150.011.67094319
GGTATCA17500.011.4832211
TAGACTG1850.011.2959465
GTCCTAA16500.011.1979121
TAGAAAT7600.011.1233794
ATTGCGA600.005882879711.08171817
AGGACCT18750.010.8413695
TACTGGT6400.010.6859422
GTCCTAC33150.010.6588341
TGTAGGA32700.010.6024292
GGACCTG18650.010.5939066
TGCGCCA909.530873E-510.55401712
ACCTTTT7200.010.55401715
ATTTAGA6950.010.5517681
CTGTAGG31700.010.54551
Produced by FastQC (version 0.11.5)