##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512905_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1915137 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.73533172822623 31.0 30.0 34.0 20.0 34.0 2 28.91059490783166 31.0 30.0 34.0 18.0 34.0 3 29.008186881669562 31.0 30.0 34.0 22.0 34.0 4 32.4071212659982 35.0 33.0 37.0 22.0 37.0 5 32.59162764857031 35.0 33.0 37.0 25.0 37.0 6 32.508770912994734 36.0 33.0 37.0 23.0 37.0 7 32.445509120235265 36.0 33.0 37.0 23.0 37.0 8 32.368387222428474 37.0 33.0 37.0 22.0 37.0 9 33.63895794400087 38.0 34.0 39.0 19.0 39.0 10 33.50466520149733 38.0 34.0 39.0 18.0 39.0 11 33.55077156360093 38.0 34.0 39.0 19.0 39.0 12 33.453895465441896 38.0 34.0 39.0 17.0 39.0 13 33.42714646523982 38.0 34.0 39.0 17.0 39.0 14 34.26012499366886 39.0 33.0 41.0 17.0 41.0 15 34.44179450347416 39.0 34.0 41.0 17.0 41.0 16 34.387678270536256 39.0 34.0 41.0 17.0 41.0 17 34.15422969740546 39.0 33.0 41.0 17.0 41.0 18 34.16044752934124 39.0 33.0 41.0 17.0 41.0 19 34.27469627499234 39.0 34.0 41.0 18.0 41.0 20 34.24468902224749 39.0 34.0 41.0 16.0 41.0 21 34.134453566507254 39.0 34.0 41.0 11.0 41.0 22 34.15069626872647 39.0 34.0 41.0 10.0 41.0 23 33.82067079274224 39.0 34.0 41.0 2.0 41.0 24 33.88496593194116 39.0 34.0 41.0 2.0 41.0 25 33.59953935410365 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7262585614940456 1101 2 0.47620294893745907 1101 3 0.7293418463151866 1101 4 0.797957496152236 1101 5 0.6882023997656077 1101 6 0.6874108216457842 1101 7 0.5427899325435206 1101 8 0.7944733966597539 1101 9 0.6973419945573696 1101 10 0.7896135060996698 1101 11 0.7068269033500911 1101 12 0.6761591787330303 1101 13 1.075058908072016 1101 14 1.1221117968687508 1101 15 0.8391062290312021 1101 16 0.8470292285345025 1101 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-2.0958505801889515 2215 16 -2.086186174359426 2215 17 -2.342324152429338 2215 18 -2.1402962266252388 2215 19 -2.1870440108829996 2215 20 -2.2433850889996023 2215 21 -2.675253123930446 2215 22 -2.6420392901838845 2215 23 -2.5695445730878035 2215 24 -2.4350891434192654 2215 25 -3.2826350987618014 2216 1 -3.0943657313436432 2216 2 -3.3548534023040197 2216 3 -4.527104119377135 2216 4 -4.983394001183999 2216 5 -4.663454591688744 2216 6 -3.633495028398851 2216 7 -5.492817956971429 2216 8 -3.2835262508251617 2216 9 -3.8173252482629785 2216 10 -2.807555797796759 2216 11 -3.0411862193080985 2216 12 -3.5913853262959954 2216 13 -3.0960119986308854 2216 14 -4.173815175652127 2216 15 -3.4901737873436893 2216 16 -3.850425498863313 2216 17 -3.7421990571907635 2216 18 -3.2374392908428753 2216 19 -3.85026529506764 2216 20 -4.393183797219731 2216 21 -4.132762036057404 2216 22 -3.8436655055655784 2216 23 -3.9024325225380565 2216 24 -4.193737465056255 2216 25 -4.276470761651261 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 101626.0 3 2.0 4 955.0 5 5670.0 6 6443.0 7 6149.0 8 5219.0 9 4892.0 10 4392.0 11 4012.0 12 3915.0 13 3663.0 14 3659.0 15 3893.0 16 3917.0 17 4274.0 18 4594.0 19 5701.0 20 7463.0 21 9541.0 22 11929.0 23 14538.0 24 16566.0 25 19032.0 26 21269.0 27 23261.0 28 26063.0 29 30895.0 30 37275.0 31 46408.0 32 57839.0 33 73852.0 34 94995.0 35 123309.0 36 167187.0 37 256170.0 38 704569.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10140175604604 18.759869768826043 12.8153217583933 24.32340671673462 2 14.305670370391645 19.86240671276435 43.211540540357056 22.62038237648694 3 19.26924577261165 25.85870253734687 28.729216852841823 26.142834837199658 4 11.936617254319533 16.45156472432589 38.41222493427876 33.19959308707581 5 12.588863131585585 39.29749232720746 35.15567269645195 12.957971844755006 6 31.35659117275112 36.57434600390882 18.844389168173407 13.224673655166647 7 28.47471603617012 31.70549700463623 21.829209917935813 17.990577041257843 8 25.21353426453316 37.62813231937335 20.581091273647406 16.57724214244608 9 27.856369424754433 14.724898460869548 20.15298283607205 37.265749278303964 10 16.13252374820216 28.79286592300231 33.83784326186574 21.236767066929794 11 34.319129266432995 22.229846361116962 25.15420590296486 18.29681846948518 12 24.59306941405027 24.519896910395566 31.491616260393762 19.395417415160402 13 29.592245206819566 22.418256728847712 25.71192212179235 22.277575942540366 14 20.908408192616694 23.168845856160427 27.476967345865827 28.44577860535705 15 22.74215471960592 32.57587119990761 23.088319600488937 21.593654479997536 16 21.317140956355733 28.009875134113372 29.08983727664538 21.583146632885512 17 21.12161334361953 29.36943745805553 28.20272793224492 21.30622126608002 18 20.997994998996454 26.984982354318593 31.41940063223389 20.597622014451062 19 23.635143490443117 26.628819970177698 27.775248741332533 21.960787798046656 20 24.29157161112287 26.943980599522828 28.565948295010596 20.198499494343704 21 23.2048867465447 26.584356892616157 26.974828384852717 23.23592797598642 22 23.64817152369976 28.96260463463142 27.06197192668846 20.32725191498036 23 21.880724618820416 28.404928143865927 28.406548349820238 21.30779888749342 24 23.319272943164908 26.702703493704703 29.095286799033975 20.882736764096407 25 22.311572548318683 27.768597114519277 28.719919391337704 21.19991094582433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 238.0 1 238.0 2 554.0 3 870.0 4 870.0 5 870.0 6 1492.0 7 2114.0 8 2114.0 9 2114.0 10 2523.0 11 2932.0 12 2932.0 13 2932.0 14 4558.0 15 6184.0 16 6184.0 17 6184.0 18 12437.5 19 18691.0 20 18691.0 21 18691.0 22 31890.5 23 45090.0 24 45090.0 25 45090.0 26 69039.0 27 92988.0 28 92988.0 29 92988.0 30 124302.0 31 155616.0 32 155616.0 33 155616.0 34 175812.5 35 196009.0 36 196009.0 37 196009.0 38 208184.5 39 220360.0 40 220360.0 41 220360.0 42 230086.5 43 239813.0 44 239813.0 45 239813.0 46 251440.5 47 263068.0 48 263068.0 49 263068.0 50 248795.0 51 234522.0 52 234522.0 53 234522.0 54 202040.0 55 169558.0 56 169558.0 57 169558.0 58 143834.0 59 118110.0 60 118110.0 61 118110.0 62 97760.5 63 77411.0 64 77411.0 65 77411.0 66 58875.5 67 40340.0 68 40340.0 69 40340.0 70 29788.5 71 19237.0 72 19237.0 73 19237.0 74 13465.5 75 7694.0 76 7694.0 77 7694.0 78 5284.5 79 2875.0 80 2875.0 81 2875.0 82 1946.5 83 1018.0 84 1018.0 85 1018.0 86 662.0 87 306.0 88 306.0 89 306.0 90 187.5 91 69.0 92 69.0 93 69.0 94 43.5 95 18.0 96 18.0 97 18.0 98 12.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34101998969264335 2 0.011382997665441166 3 0.009607667754317315 4 0.012061800278517934 5 0.013262758747807597 6 0.07921104338749656 7 0.0790021810450114 8 0.10328242835891113 9 0.08297056555222942 10 0.09028074753920999 11 0.08479811104897457 12 0.09659883339938606 13 0.08276170320974427 14 0.08657344096009842 15 0.08344050582282103 16 0.08584242276140036 17 0.0988441035811015 18 0.10030613997849762 19 0.09419691646080672 20 0.09304817357713835 21 0.081090804469863 22 0.1008805114203318 23 0.09398805411832156 24 0.09001966961110354 25 0.08850541762808614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1915137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.0674078155209 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87914082936501 45.222398308611226 2 12.933044541197589 15.019767433400338 3 4.262115735781023 7.424700377596369 4 1.8282436258322388 4.246454728293089 5 0.9300955805189837 2.700411969070474 6 0.5561714307491425 1.9377259970791323 7 0.35074562499864226 1.4256822472414146 8 0.2392162477641565 1.111253393201597 9 0.1647067067754095 0.860768236105121 >10 0.7577279919118605 7.691832350909043 >50 0.051802670498120956 2.0875530855522344 >100 0.03902323654325638 4.431469005864037 >500 0.004978611290288271 2.027975509450725 >1k 0.0026250859530610876 2.6161525337302747 >5k 3.6208082111187416E-4 1.1958548238949103 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6528 0.3408633429357795 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5655 0.29527913668839356 No Hit GTATCAACGCAGAGTACTTTTTTTT 5363 0.28003218568697696 No Hit CTGTAGGACGTGGAATATGGCAAGA 5205 0.27178212315881317 No Hit CTTTAGGACGTGAAATATGGCGAGG 4597 0.240035047101069 No Hit GTCCTACAGTGTGCATTTCTCATTT 3532 0.1844254484143954 No Hit TATCAACGCAGAGTACTTTTTTTTT 3070 0.16030184785735957 No Hit GGTATCAACGCAGAGTACTTTTTTT 2983 0.15575909190830736 No Hit CTGTAGGACCTGGAATATGGCGAGA 2580 0.13471621090292757 No Hit GTACTGGTTCACTATCGGTCAGTCA 2420 0.1263617172035212 No Hit CTGAAGGACCTGGAATATGGCGAGA 2238 0.11685848062044649 No Hit GTCCTTCAGTGTGCATTTCTCATTT 1925 0.10051500232098277 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 5.221558562128976E-5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 100 6.6211214E-10 14.247923 19 TCCAACG 160 0.0 13.654259 18 TAGGACC 1070 0.0 13.227043 4 TATTGCG 55 0.003072244 12.089147 16 CTAATAC 145 9.822543E-11 11.791384 3 GGCGAGG 1115 0.0 11.670943 19 GGTATCA 1750 0.0 11.483221 1 TAGACTG 185 0.0 11.295946 5 GTCCTAA 1650 0.0 11.197912 1 TAGAAAT 760 0.0 11.123379 4 ATTGCGA 60 0.0058828797 11.081718 17 AGGACCT 1875 0.0 10.841369 5 TACTGGT 640 0.0 10.685942 2 GTCCTAC 3315 0.0 10.658834 1 TGTAGGA 3270 0.0 10.602429 2 GGACCTG 1865 0.0 10.593906 6 TGCGCCA 90 9.530873E-5 10.554017 12 ACCTTTT 720 0.0 10.554017 15 ATTTAGA 695 0.0 10.551768 1 CTGTAGG 3170 0.0 10.5455 1 >>END_MODULE