FastQCFastQC Report
Thu 26 May 2016
SRR1512905_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512905_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915137
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68370.3569979588927581No Hit
GTCCTACAGTGGACATTTCTAAATT61250.31982046193039976No Hit
GTCCTAAAGTGTGTATTTCTCATTT53360.27862236487520214No Hit
CTGTAGGACGTGGAATATGGCAAGA51480.26880583477839964No Hit
CTTTAGGACGTGAAATATGGCGAGG49970.2609212813495849No Hit
TATCAACGCAGAGTACTTTTTTTTT47170.24630091737562376No Hit
GGTATCAACGCAGAGTACTTTTTTT40330.2105854568106616No Hit
GTCCTACAGTGTGCATTTCTCATTT33130.17299023516333295No Hit
CTGTAGGACCTGGAATATGGCGAGA26810.13998998505067783No Hit
CTGAAGGACCTGGAATATGGCGAGA23960.12510854314861025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23900.12479524963488252No Hit
GTACTGGTTCACTATCGGTCAGTCA22700.11852937936032776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT651.8893661E-716.0952076
ATACCGT551.9417099E-413.8338996
TAGGACC11900.013.34744554
GGTATCA17150.012.3710271
AGGACCT19600.011.9858685
GGCGAGG13600.011.72471519
AGGACCG1156.9629095E-811.5786745
TGGCGAG30400.011.395967518
GGACCTG20550.011.2463536
TAGAACC1950.011.2181664
CACCTTT6600.011.0742114
TAATACT1855.456968E-1210.7963324
TTAGGAC18550.010.717083
TGTAGGA33100.010.575332
GTAGGAC32400.010.5689453
CCTACAC3150.010.5689443
GGCGAGA18300.010.47688619
AGGACGT32150.010.442935
AATGTCC7100.010.437848
AAATGTC6850.010.4086617
Produced by FastQC (version 0.11.5)