##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512905_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1915137 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.192481791119903 31.0 30.0 34.0 26.0 34.0 2 30.19319818895463 31.0 30.0 34.0 26.0 34.0 3 30.376127138685117 31.0 30.0 34.0 26.0 34.0 4 33.97158166752561 37.0 35.0 37.0 30.0 37.0 5 33.55449871210258 37.0 35.0 37.0 28.0 37.0 6 33.431204138398456 37.0 35.0 37.0 28.0 37.0 7 33.2663470028515 37.0 35.0 37.0 27.0 37.0 8 33.20306171307849 37.0 35.0 37.0 26.0 37.0 9 34.515307782158665 39.0 35.0 39.0 27.0 39.0 10 34.42558260845047 39.0 35.0 39.0 27.0 39.0 11 34.42100852315004 39.0 35.0 39.0 27.0 39.0 12 34.28453212485582 39.0 35.0 39.0 25.0 39.0 13 34.278790499060904 39.0 35.0 39.0 25.0 39.0 14 35.34009211873615 39.0 35.0 41.0 25.0 41.0 15 35.345761687022915 40.0 36.0 41.0 25.0 41.0 16 35.342260109851146 40.0 36.0 41.0 26.0 41.0 17 35.34658982621087 40.0 36.0 41.0 26.0 41.0 18 35.26889251265053 40.0 36.0 41.0 25.0 41.0 19 35.28414259658709 40.0 35.0 41.0 25.0 41.0 20 35.255428723898085 40.0 35.0 41.0 25.0 41.0 21 35.226165021092484 40.0 35.0 41.0 25.0 41.0 22 35.18699184444768 40.0 35.0 41.0 25.0 41.0 23 35.02329076196638 40.0 35.0 41.0 24.0 41.0 24 34.98933653310442 40.0 35.0 41.0 24.0 41.0 25 34.92500693161899 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6143130352748543 1101 2 0.4393609044296234 1101 3 0.39036987224194775 1101 4 0.4565992192272006 1101 5 0.5049908358457529 1101 6 0.5598825670359915 1101 7 0.621003554726201 1101 8 0.42614329359147973 1101 9 0.8035747394888375 1101 10 0.45955846144830304 1101 11 0.6639291178357567 1101 12 0.693740975548188 1101 13 0.8673812727905741 1101 14 0.9835922791913205 1101 15 0.6156392056951816 1101 16 0.9319164528087285 1101 17 0.9153532854535413 1101 18 0.814715524135849 1101 19 0.5653782938298875 1101 20 0.6980632146751802 1101 21 0.7015594667423386 1101 22 0.6716646097796684 1101 23 0.8601103186182257 1101 24 0.8725959538612926 1101 25 0.9770948336732346 1102 1 0.7845921678670713 1102 2 0.8537943031331459 1102 3 0.5276191248176652 1102 4 0.6741450678434049 1102 5 0.8126914818237907 1102 6 0.8102151637772721 1102 7 0.7770100201581798 1102 8 0.8738546776121439 1102 9 1.001858235117325 1102 10 0.8644817974755625 1102 11 1.149734555806603 1102 12 1.1301577472404531 1102 13 1.1159947654237072 1102 14 1.1516996144047553 1102 15 1.2473037718499995 1102 16 1.2789028494042043 1102 17 1.2878974668473404 1102 18 0.7167150607045372 1102 19 1.0827200066042053 1102 20 1.166906049292372 1102 21 1.1338385880313524 1102 22 0.9833829791796376 1102 23 1.2247367195089183 1102 24 1.1463902694806194 1102 25 1.1847087840860837 1103 1 0.6221239813908852 1103 2 0.850425658884209 1103 3 0.5803659778312742 1103 4 0.5224467050746853 1103 5 0.6603463278114177 1103 6 0.6304148596021761 1103 7 0.6417634727742509 1103 8 0.6063670246626671 1103 9 0.7411036512550382 1103 10 0.7085771263991703 1103 11 0.7014243909242524 1103 12 0.9198083544969293 1103 13 0.8983460142637867 1103 14 0.9419207304011294 1103 15 0.9574551298839822 1103 16 1.0306574857870459 1103 17 0.9950136251138844 1103 18 0.9395167116488992 1103 19 0.9084088226231302 1103 20 0.8389982118813677 1103 21 1.0088132299283075 1103 22 0.9172197326229323 1103 23 0.9576676765145677 1103 24 0.9653996473429487 1103 25 0.8715379047264733 1104 1 0.8152053648392403 1104 2 0.8955578966769977 1104 3 0.7865588675104007 1104 4 0.6912519059899793 1104 5 0.7953993481855406 1104 6 0.5774303823057778 1104 7 0.5804417669571365 1104 8 0.6122174433571246 1104 9 0.5991355276355392 1104 10 0.7898887811053186 1104 11 0.8547970121867792 1104 12 0.9243092053280506 1104 13 0.7207819880759061 1104 14 1.033072697699886 1104 15 0.975349315209364 1104 16 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16 0.6384323634236182 1107 17 0.6904127901174206 1107 18 0.826080560580067 1107 19 0.6282158730831284 1107 20 0.7114705449519576 1107 21 0.653971545398619 1107 22 0.6630464372788367 1107 23 0.7622956635163121 1107 24 0.8755127999778125 1107 25 0.8902622917364837 1108 1 0.769288509369364 1108 2 0.6752368296256108 1108 3 0.6773836242675237 1108 4 0.6951909504933482 1108 5 0.8223190450166911 1108 6 0.884245331455908 1108 7 0.7978351143110132 1108 8 0.7755224816088244 1108 9 0.9535326950794314 1108 10 1.0251223382932224 1108 11 0.8748059189569375 1108 12 0.9283744008536701 1108 13 0.9687788114407709 1108 14 1.148574541706104 1108 15 1.20577431715666 1108 16 1.1496521861771 1108 17 1.1014127169780892 1108 18 1.2663852565407012 1108 19 1.315820855035163 1108 20 1.2743348390784561 1108 21 1.3841000877733052 1108 22 1.3458863502430347 1108 23 1.264642355582268 1108 24 1.240871914094285 1108 25 1.2512576681671703 1109 1 0.02000181707162696 1109 2 0.09845183841281013 1109 3 0.24928994562438334 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2216 23 -3.362134292733895 2216 24 -2.6753753218453014 2216 25 -2.709945802218485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55700.0 3 0.0 4 893.0 5 7863.0 6 9325.0 7 7358.0 8 5892.0 9 4957.0 10 4364.0 11 3886.0 12 3445.0 13 3239.0 14 3126.0 15 2922.0 16 3323.0 17 3415.0 18 3730.0 19 4716.0 20 5966.0 21 7884.0 22 9986.0 23 11944.0 24 14362.0 25 16203.0 26 18374.0 27 20163.0 28 22827.0 29 27046.0 30 33877.0 31 43673.0 32 55328.0 33 71099.0 34 92226.0 35 120498.0 36 165019.0 37 257076.0 38 793432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.989788458031036 19.718780196297818 13.852421792078582 26.43900955359257 2 12.984696875906002 20.447575242319054 41.861553610560435 24.706174271214508 3 17.40748768112725 25.85352096493267 29.496094780244892 27.24289657369519 4 11.268883910620943 17.15429323390723 37.66698520618907 33.909837649282764 5 12.227068990190517 39.34833951191793 35.3231196054898 13.10147189240175 6 31.532189554264722 37.140217951249895 18.26352686459708 13.064065629888308 7 28.137063947183723 31.863113737594073 21.80662565632315 18.193196658899055 8 25.197014543260128 38.03876286936145 20.14080864392655 16.623413943451876 9 27.22540680714821 14.6030121095474 20.126240138305292 38.045340944999104 10 15.623406585139552 29.38272675599542 33.71279245693078 21.28107420193425 11 34.17061101549053 22.19970380829838 25.121568103203384 18.508117073007703 12 24.2593579858067 24.88307576147612 31.43788225231825 19.41968400039893 13 29.42965414136899 22.319273160591134 25.896917595589837 22.354155102450036 14 20.718111311796157 23.171290168121157 27.446038263136213 28.66456025694648 15 22.68481309667982 32.58043509026998 23.042331399517565 21.692420413532638 16 21.33918132014177 28.077751975105496 28.91583692827165 21.667229776481086 17 20.925135781375406 29.46344881821509 28.22983696835606 21.381578432053438 18 20.886863518848944 26.975558225646346 31.58099487165027 20.556583383854445 19 23.48913421482183 26.58517023185285 28.02812812321484 21.897567430110477 20 24.081911426755774 26.955348904726822 28.743147199639914 20.21959246887749 21 22.76001215588354 26.43241527171637 27.403632771175936 23.40393980122415 22 23.396340699277328 28.7323509753958 27.373480512970467 20.497827812356405 23 21.604564059907986 28.263930987704793 28.616125112720397 21.515379839666824 24 23.21285631262933 26.7013795879877 29.224488900794043 20.861275198588928 25 22.052805676884397 27.773498996154316 28.884543387280793 21.289151939680494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 102.0 1 102.0 2 304.5 3 507.0 4 507.0 5 507.0 6 1195.5 7 1884.0 8 1884.0 9 1884.0 10 2390.0 11 2896.0 12 2896.0 13 2896.0 14 4499.5 15 6103.0 16 6103.0 17 6103.0 18 12610.0 19 19117.0 20 19117.0 21 19117.0 22 32854.5 23 46592.0 24 46592.0 25 46592.0 26 71239.0 27 95886.0 28 95886.0 29 95886.0 30 127036.5 31 158187.0 32 158187.0 33 158187.0 34 177612.5 35 197038.0 36 197038.0 37 197038.0 38 208853.5 39 220669.0 40 220669.0 41 220669.0 42 230535.5 43 240402.0 44 240402.0 45 240402.0 46 251505.0 47 262608.0 48 262608.0 49 262608.0 50 247832.5 51 233057.0 52 233057.0 53 233057.0 54 200967.5 55 168878.0 56 168878.0 57 168878.0 58 142903.5 59 116929.0 60 116929.0 61 116929.0 62 96176.0 63 75423.0 64 75423.0 65 75423.0 66 57352.0 67 39281.0 68 39281.0 69 39281.0 70 28835.0 71 18389.0 72 18389.0 73 18389.0 74 12882.0 75 7375.0 76 7375.0 77 7375.0 78 4990.0 79 2605.0 80 2605.0 81 2605.0 82 1749.0 83 893.0 84 893.0 85 893.0 86 578.5 87 264.0 88 264.0 89 264.0 90 153.5 91 43.0 92 43.0 93 43.0 94 26.0 95 9.0 96 9.0 97 9.0 98 4.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08438038636400425 2 0.04292121138070018 3 0.020468509563545585 4 0.007675691086329595 5 0.023966953800171997 6 0.05905582733767872 7 0.08542469807643004 8 0.06224097806057739 9 0.027778691550526154 10 0.05268552589188136 11 0.00840670928502765 12 4.17724684970318E-4 13 0.005534852075856714 14 0.0015664675686386925 15 0.014098208117748236 16 0.009189943069346996 17 0.005743714418341873 18 0.005012696219643817 19 0.0022452701817154593 20 0.002297485767336749 21 7.832337843193463E-4 22 8.87664955561926E-4 23 0.0 24 0.0 25 1.044311712425795E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1915137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.61304969032672 #Duplication Level Percentage of deduplicated Percentage of total 1 76.60351288650487 43.36758481497284 2 13.655237368650539 15.461292633692386 3 4.489055416422605 7.624173520576899 4 1.9476072185241233 4.410399369581809 5 1.017025704101326 2.878846336131395 6 0.5812810297595137 1.974485509309178 7 0.359662093082485 1.4253097563183945 8 0.2535428502651186 1.148306718454898 9 0.17746462105880179 0.9042132069249254 >10 0.812950663201947 8.008700611628813 >50 0.05475839778016664 2.1857935202119054 >100 0.03967298915911452 4.440928665718931 >500 0.0049927316902437175 1.987841133263291 >1k 0.0028661978221769476 2.9548834203591454 >5k 3.6983197705509E-4 1.2272407828551781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6837 0.3569979588927581 No Hit GTCCTACAGTGGACATTTCTAAATT 6125 0.31982046193039976 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5336 0.27862236487520214 No Hit CTGTAGGACGTGGAATATGGCAAGA 5148 0.26880583477839964 No Hit CTTTAGGACGTGAAATATGGCGAGG 4997 0.2609212813495849 No Hit TATCAACGCAGAGTACTTTTTTTTT 4717 0.24630091737562376 No Hit GGTATCAACGCAGAGTACTTTTTTT 4033 0.2105854568106616 No Hit GTCCTACAGTGTGCATTTCTCATTT 3313 0.17299023516333295 No Hit CTGTAGGACCTGGAATATGGCGAGA 2681 0.13998998505067783 No Hit CTGAAGGACCTGGAATATGGCGAGA 2396 0.12510854314861025 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2390 0.12479524963488252 No Hit GTACTGGTTCACTATCGGTCAGTCA 2270 0.11852937936032776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 1.0443117124257951E-4 0.0 0.0 0.0 0.0 11 1.5664675686386927E-4 0.0 0.0 0.0 0.0 12 1.5664675686386927E-4 0.0 0.0 0.0 0.0 13 1.5664675686386927E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 65 1.8893661E-7 16.095207 6 ATACCGT 55 1.9417099E-4 13.833899 6 TAGGACC 1190 0.0 13.3474455 4 GGTATCA 1715 0.0 12.371027 1 AGGACCT 1960 0.0 11.985868 5 GGCGAGG 1360 0.0 11.724715 19 AGGACCG 115 6.9629095E-8 11.578674 5 TGGCGAG 3040 0.0 11.3959675 18 GGACCTG 2055 0.0 11.246353 6 TAGAACC 195 0.0 11.218166 4 CACCTTT 660 0.0 11.07421 14 TAATACT 185 5.456968E-12 10.796332 4 TTAGGAC 1855 0.0 10.71708 3 TGTAGGA 3310 0.0 10.57533 2 GTAGGAC 3240 0.0 10.568945 3 CCTACAC 315 0.0 10.568944 3 GGCGAGA 1830 0.0 10.476886 19 AGGACGT 3215 0.0 10.44293 5 AATGTCC 710 0.0 10.43784 8 AAATGTC 685 0.0 10.408661 7 >>END_MODULE