FastQCFastQC Report
Thu 26 May 2016
SRR1512904_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512904_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608121
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT115700.7194732237188619No Hit
TATCAACGCAGAGTACTTTTTTTTT67640.4206151154048731No Hit
GGTATCAACGCAGAGTACTTTTTTT65590.4078673184418337No Hit
GTCCTACAGTGGACATTTCTAAATT64560.40146232777259916No Hit
CTGTAGGACGTGGAATATGGCAAGA60120.3738524650819186No Hit
GTCCTAAAGTGTGTATTTCTCATTT55180.3431333836197649No Hit
CTTTAGGACGTGAAATATGGCGAGG46360.28828676449098045No Hit
GTACTGGTTCACTATCGGTCAGTCA37490.23312922348504872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32040.19923873887599253No Hit
GTCCTACAGTGTGCATTTCTCATTT27780.17274819494304222No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24460.15210298230046124No Hit
CTGAAGGACCTGGAATATGGCGAGA23280.14476522599978484No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22390.1392308165865628No Hit
GGATACCACGTGTCCCGCCCTACTC21530.1338829602996292No Hit
GTCCTTCAGTGTGCATTTCTCATTT20910.13002752902300263No Hit
ATTTAGAAATGTCCACTGTAGGACG18270.11361085390962497No Hit
CTGTAGGACCTGGAATATGGCGAGA18070.11236716640103575No Hit
TTTCTAAATTTTCCACCTTTTTCAG17230.10714367886496103No Hit
GAATATGGCAAGAAAACTGAAAATC16980.10558906947922451No Hit
ATCATTAACTGAATCCATAGGTTAA16770.10428319759520582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTTT6850.012.75761914
ACGTGTA604.0962317E-412.6651748
GGTATCA20050.012.5879391
GTCTAGA1301.891749E-1012.4545691
TAGGACC7450.012.3672844
GGCGAGG12550.011.73058819
TGGCGAA1302.6193447E-911.69092818
TTCCACC7200.011.60901811
AATCCCG1701.0913936E-1111.174108519
AATGTCC5550.011.1248148
TTTCCAC5900.011.10816710
TAGGCCT1201.2777309E-711.0816814
CCACCTT8100.011.02339113
TAGAAAT6100.010.9000144
GTATCAA43100.010.7836481
TCCACCT8200.010.77311812
ATTTCTA17850.010.74943215
GTGTAGA1605.2023097E-1010.7145921
TGGCGAG26300.010.69075418
TGTAGGA29150.010.6553572
Produced by FastQC (version 0.11.5)