FastQCFastQC Report
Thu 26 May 2016
SRR1512904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608121
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142090.8835777904772092No Hit
TATCAACGCAGAGTACTTTTTTTTT104010.6467796888418222No Hit
GGTATCAACGCAGAGTACTTTTTTT84100.5229705973617658No Hit
GTCCTACAGTGGACATTTCTAAATT59340.36900208379842064No Hit
CTGTAGGACGTGGAATATGGCAAGA57130.3552593368285098No Hit
GTCCTAAAGTGTGTATTTCTCATTT52340.32547302099779807No Hit
CTTTAGGACGTGAAATATGGCGAGG50810.31595881155709055No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49460.30756392087411333No Hit
GTACTGGTTCACTATCGGTCAGTCA36000.22386375154605903No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29330.18238677313460866No Hit
GTCCTACAGTGTGCATTTCTCATTT26450.16447767301092392No Hit
CTGAAGGACCTGGAATATGGCGAGA24480.15222735105132013No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24430.15191642917417283No Hit
GGATACCACGTGTCCCGCCCTACTC20190.12555025399208145No Hit
ATTTAGAAATGTCCACTGTAGGACG18960.11790157581425777No Hit
TTTCTAAATTTTCCACCTTTTTCAG18960.11790157581425777No Hit
GTCCTTCAGTGTGCATTTCTCATTT18760.11665788830566855No Hit
CTGTAGGACCTGGAATATGGCGAGA17910.11137221639416436No Hit
ATCATTAACTGAATCCATAGGTTAA17530.10900921012784485No Hit
GCAGAGTACTTTTTTTTTTTTTTTT16320.1014849007008801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC402.7332586E-416.6471713
TGGTTCG508.620305E-515.2193225
TGTACGA551.9381802E-413.836612
GGATCGT500.001485645813.3164936
TAGGACC6550.013.0700284
GGTATCA19850.013.0333691
ATAAGAC853.8852177E-612.31051353
GCAACCG550.003072253412.0889479
GGCGAGG12850.012.03888919
CTACACG1052.675406E-711.7768564
AATACGG658.082978E-411.6820514
ATAATAC1104.8831134E-711.2422463
AGAACCG855.254442E-511.1906795
TTTAGAA7300.010.9460862
TATGTCG1053.502484E-610.84761716
AGGACCG1158.6722685E-710.7527835
GTATCAA44050.010.7314581
TAAGACT1605.311449E-1010.7010864
AAATGTC6000.010.6161947
AGGACCT14250.010.6134755
Produced by FastQC (version 0.11.5)