FastQCFastQC Report
Thu 26 May 2016
SRR1512903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622204
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT113490.432803855077637No Hit
CTGTAGGACGTGGAATATGGCAAGA92500.3527566886481754No Hit
GTCCTAAAGTGTGTATTTCTCATTT79190.3019978613410703No Hit
CTTTAGGACGTGAAATATGGCGAGG64030.2441839002610018No Hit
GTATCAACGCAGAGTACTTTTTTTT63870.24357372652928605No Hit
GTCCTACAGTGTGCATTTCTCATTT62280.23751012507036065No Hit
CTGTAGGACCTGGAATATGGCGAGA42890.16356469595805667No Hit
CTGAAGGACCTGGAATATGGCGAGA36820.1404162300110899No Hit
TATCAACGCAGAGTACTTTTTTTTT36020.1373653613525111No Hit
GGTATCAACGCAGAGTACTTTTTTT34940.1332466886634297No Hit
GTCCTTCAGTGTGCATTTCTCATTT29270.11162365704575235No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT456.7703973E-414.77608517
TAGGACC15500.013.9107794
TGGACCG1103.824971E-812.08929355
GGTATCA19700.012.0374881
TAGAAAT10150.011.8848844
TGTAGGA48700.011.6635162
GGACCTG28450.011.5520596
AGGACCT29300.011.5411215
GTGTAGG4600.011.3869541
GTCCTAC49850.011.3863321
GGCGAGG19000.011.34826719
TCCAACG2450.011.2436118
CTATAGT3550.011.23772054
AATGTCC10350.011.1965718
GTATCAA44400.011.1752561
TACCGTA600.00588332911.08185312
CTGTAGG48650.011.0799121
TTAGAAA9800.011.0493343
GTAGGAC48200.010.976543
TCCCGCC3550.010.97057413
Produced by FastQC (version 0.11.5)