##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512903_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2622204
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.682960593454972	31.0	30.0	34.0	18.0	34.0
2	28.87351518035973	31.0	30.0	34.0	16.0	34.0
3	28.97902337117936	31.0	30.0	34.0	20.0	34.0
4	32.37409789627352	35.0	33.0	37.0	22.0	37.0
5	32.53398553278082	35.0	33.0	37.0	25.0	37.0
6	32.4789383282155	36.0	33.0	37.0	23.0	37.0
7	32.415692676847414	37.0	33.0	37.0	23.0	37.0
8	32.347797501643655	37.0	33.0	37.0	22.0	37.0
9	33.6266526174165	38.0	34.0	39.0	19.0	39.0
10	33.49172795099084	38.0	34.0	39.0	18.0	39.0
11	33.54357288754041	38.0	34.0	39.0	18.0	39.0
12	33.43980331049758	38.0	34.0	39.0	17.0	39.0
13	33.4096439483732	38.0	34.0	39.0	17.0	39.0
14	34.26281822466902	39.0	34.0	41.0	17.0	41.0
15	34.43992115029952	39.0	34.0	41.0	17.0	41.0
16	34.3775541491051	39.0	34.0	41.0	17.0	41.0
17	34.153582253707185	39.0	33.0	41.0	16.0	41.0
18	34.14009398200903	39.0	34.0	41.0	16.0	41.0
19	34.243264444719024	39.0	34.0	41.0	16.0	41.0
20	34.22508622517546	39.0	34.0	41.0	15.0	41.0
21	34.12768571781601	39.0	34.0	41.0	10.0	41.0
22	34.130772434181324	39.0	34.0	41.0	10.0	41.0
23	33.79858737153936	39.0	34.0	41.0	2.0	41.0
24	33.85873486578466	39.0	34.0	41.0	2.0	41.0
25	33.57246156286849	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.636160792167896
1101	2	0.2929749394371228
1101	3	0.6298273558505336
1101	4	0.6793598008058552
1101	5	0.6352718625310843
1101	6	0.5600524892548719
1101	7	0.2883865365650422
1101	8	0.5404498582823791
1101	9	0.5463221705814831
1101	10	0.5776003130904641
1101	11	0.5799856682948601
1101	12	0.49299341338095104
1101	13	0.9121221823583312
1101	14	0.9974512994664124
1101	15	0.819381691904006
1101	16	0.620230069211992
1101	17	0.15803416633001177
1101	18	0.41062533470581286
1101	19	-0.029761903113239896
1101	20	-0.566270822776545
1101	21	0.4701467676828557
1101	22	0.40575093770465287
1101	23	0.018123794866077958
1101	24	0.554826091697521
1101	25	0.15646039135997825
1102	1	0.9602202036162844
1102	2	0.9369222281562415
1102	3	0.9098597441141152
1102	4	1.0337084196689332
1102	5	0.9000147034268267
1102	6	0.7931552725440127
1102	7	0.8815472973183773
1102	8	0.9248299382640823
1102	9	1.1939761848100616
1102	10	1.2520339820502642
1102	11	1.1188735270226928
1102	12	1.210247889945812
1102	13	1.048880080088061
1102	14	1.2968013708565778
1102	15	1.259025791862598
1102	16	1.2016269057934252
1102	17	1.4291733802347153
1102	18	1.4086035406185289
1102	19	1.233291988732212
1102	20	1.4182643994600994
1102	21	1.5688580362025775
1102	22	1.4069185222448155
1102	23	1.349525267172254
1102	24	0.7137282284575832
1102	25	1.1488989061973882
1103	1	0.7495981433337846
1103	2	0.8168705295734888
1103	3	0.9244391964850429
1103	4	1.043911038619683
1103	5	0.9195591111533048
1103	6	0.5677177497935446
1103	7	0.3844752975848422
1103	8	0.6408986690674467
1103	9	0.7370358939371329
1103	10	0.9190452068046824
1103	11	0.5930143027631445
1103	12	0.8997540055098696
1103	13	1.0093933544664537
1103	14	0.9721152381151725
1103	15	1.0185878289723291
1103	16	0.9026370266296411
1103	17	0.6516575054628149
1103	18	1.0526349475670287
1103	19	0.8538738773363974
1103	20	1.0457503566214115
1103	21	0.8738524333419093
1103	22	0.8859148131748427
1103	23	0.28544638460392946
1103	24	0.4983697286443487
1103	25	0.7678096910499193
1104	1	0.9801606466877821
1104	2	0.9600055084462191
1104	3	0.9565987277886343
1104	4	1.169547773237376
1104	5	0.9131676745222777
1104	6	0.9330259209489284
1104	7	1.0477642453744664
1104	8	0.8275663817925718
1104	9	1.084057893829808
1104	10	1.1527923165334926
1104	11	0.9752615046539788
1104	12	1.2538122845764903
1104	13	1.2354229443104359
1104	14	1.4856555899174637
1104	15	1.1615381921373853
1104	16	1.2191441207725688
1104	17	1.4775824505739124
1104	18	1.2704534257036286
1104	19	0.8144779935958795
1104	20	1.0028837559069856
1104	21	1.0154050251947808
1104	22	0.9750637260128983
1104	23	1.3392314440892719
1104	24	1.1349286551783777
1104	25	0.6577257652741793
1105	1	0.8453084180027837
1105	2	0.8787136523628334
1105	3	0.9734279824102465
1105	4	1.115411063897696
1105	5	0.8662077617756125
1105	6	1.0565633591902213
1105	7	1.0526658226372234
1105	8	1.100887087993378
1105	9	0.8191154691167526
1105	10	0.8726719928834257
1105	11	0.9328642918093379
1105	12	1.0960461893615303
1105	13	1.0356230360945844
1105	14	1.1704722610020681
1105	15	1.0547791535588473
1105	16	1.04879472209889
1105	17	1.3522642713566029
1105	18	1.197723944891301
1105	19	0.9204594359149141
1105	20	1.2293775546381198
1105	21	1.0923115606700549
1105	22	1.0661587366217944
1105	23	1.3805642643966891
1105	24	1.3894865673575296
1105	25	1.6032456243983546
1106	1	0.8201367467069467
1106	2	0.7112080133161065
1106	3	0.6696135964485066
1106	4	0.768251134009013
1106	5	0.7127622411333903
1106	6	0.76900304627015
1106	7	0.901410090142079
1106	8	0.8320601478017906
1106	9	0.8964490964058811
1106	10	0.7051379026615336
1106	11	0.5557670592634807
1106	12	0.794955140363129
1106	13	0.8800692714684217
1106	14	1.1218192039173474
1106	15	0.9020100328215861
1106	16	0.9270253589909458
1106	17	1.044888012540902
1106	18	0.9389815773059311
1106	19	0.937568353679822
1106	20	1.0221602652756232
1106	21	1.1210928408919898
1106	22	0.9173801610471344
1106	23	1.308681477524324
1106	24	1.0785858150560799
1106	25	1.2572027900232357
1107	1	1.1123615221035585
1107	2	1.0786302096532339
1107	3	1.0402361719935413
1107	4	1.1926435389870917
1107	5	1.0798748918352246
1107	6	1.2136105106027628
1107	7	0.9848615970965824
1107	8	1.1560851370831813
1107	9	1.1770084002195205
1107	10	1.283287787745472
1107	11	1.1124494264437956
1107	12	1.2240762698530219
1107	13	0.9848023600281834
1107	14	1.4502685950049852
1107	15	1.1577253687592588
1107	16	1.1920301977451757
1107	17	1.509814066301793
1107	18	1.3947899046058083
1107	19	1.2976317902236687
1107	20	1.43176655619002
1107	21	1.2500524309686085
1107	22	1.2070376923841337
1107	23	1.5259630145605811
1107	24	1.3759259278295772
1107	25	1.6722357049263934
1108	1	0.9070143537611735
1108	2	0.8597137653936464
1108	3	0.8496259722848727
1108	4	0.8705901995036811
1108	5	0.8323504759370977
1108	6	0.8609188739088367
1108	7	0.824453878926569
1108	8	0.8021933686780471
1108	9	0.9241172192712597
1108	10	0.9548727392513996
1108	11	0.8050471811321174
1108	12	0.9865665048268752
1108	13	0.8632847749824677
1108	14	1.0872013680768902
1108	15	1.041377026391892
1108	16	0.7767281907045813
1108	17	1.0936536479187495
1108	18	1.064589600479124
1108	19	1.09876964350876
1108	20	1.0542408693942917
1108	21	1.0530315304798918
1108	22	1.051521930143302
1108	23	1.449580513487433
1108	24	1.2199672400002441
1108	25	1.4534039131118632
1109	1	0.8136014708715038
1109	2	0.6493417253912313
1109	3	0.8380380794897917
1109	4	0.8711389634103597
1109	5	0.6012432476570382
1109	6	0.7753190866932655
1109	7	0.6481094431645005
1109	8	0.5344696883007174
1109	9	0.6959678951086303
1109	10	0.7839021240408357
1109	11	0.7311115880880692
1109	12	0.6325023905156257
1109	13	0.7371488485702713
1109	14	0.8286717005591697
1109	15	0.7668133270279114
1109	16	0.40518015312088806
1109	17	0.9302509723352301
1109	18	1.038970077375403
1109	19	0.8088224297375319
1109	20	0.662739663777451
1109	21	0.7028240788819602
1109	22	0.6951705374586297
1109	23	1.0603287936488925
1109	24	0.7620027425624301
1109	25	0.37301008341962216
1110	1	0.5424462526712546
1110	2	0.694732029775416
1110	3	0.5787263986445836
1110	4	0.38277164609731784
1110	5	0.37999495190829435
1110	6	0.42022428581071836
1110	7	0.39886516303853625
1110	8	0.40815534855816793
1110	9	0.6146051332085065
1110	10	0.576783744862368
1110	11	0.5181556507768263
1110	12	0.491045692546237
1110	13	0.5205736635191016
1110	14	0.5237763429677642
1110	15	0.5132394539431004
1110	16	0.5716674642771054
1110	17	0.6363483465366357
1110	18	0.053594094802768666
1110	19	0.40177278693743546
1110	20	0.5401734866828392
1110	21	0.7103085304363006
1110	22	0.6661771040754303
1110	23	0.8075519386243784
1110	24	0.5629965911435519
1110	25	0.949011467996371
1111	1	0.4694979244037789
1111	2	0.4892141434064641
1111	3	0.5212094475106106
1111	4	0.5064564719098001
1111	5	0.4931583842206635
1111	6	0.44477420397765854
1111	7	0.3210068511376747
1111	8	0.5051859775036931
1111	9	0.6321407890420332
1111	10	0.6104522040627387
1111	11	0.2829440538631047
1111	12	-0.07079672036000062
1111	13	0.37000083209520795
1111	14	0.4398390037112776
1111	15	0.4821428909316481
1111	16	0.5840593277327955
1111	17	0.12267053498648295
1111	18	0.5366429608303633
1111	19	0.5154038367386988
1111	20	0.7162314712514615
1111	21	0.721063732680868
1111	22	0.23737186675955968
1111	23	0.6733457193117758
1111	24	0.7715539911903093
1111	25	0.8898578556512717
1112	1	0.3207905135447362
1112	2	0.2829246881808416
1112	3	0.21362427471716927
1112	4	0.470535161175782
1112	5	0.30826358169025525
1112	6	0.2540530082819288
1112	7	0.06079906870932916
1112	8	0.259951120461821
1112	9	0.16925726860682033
1112	10	0.23783340342475867
1112	11	0.09464228471027525
1112	12	0.1465947534144476
1112	13	0.20920265514480008
1112	14	0.2664936945403795
1112	15	-0.049914318665265967
1112	16	0.20084558812109066
1112	17	0.5133659897664486
1112	18	0.22311855196582542
1112	19	0.3982219126793396
1112	20	0.16730550958951085
1112	21	0.128109114628856
1112	22	0.0707024558588003
1112	23	0.26013195774620357
1112	24	0.1993232252526269
1112	25	0.2047440632405042
1113	1	-0.08097076048517238
1113	2	0.060376855775899685
1113	3	0.10922677483939935
1113	4	0.0440361737093653
1113	5	-0.025532583530662123
1113	6	-0.3163116516238986
1113	7	-0.3270321650807517
1113	8	-0.19382493053212357
1113	9	-0.21863923124480067
1113	10	-0.18547760982515626
1113	11	-0.22329587626494174
1113	12	-0.3623218527048735
1113	13	-0.32109421447202635
1113	14	0.023718442885133584
1113	15	-0.3275735979869552
1113	16	-0.21322243184986434
1113	17	-0.12744457151030986
1113	18	-0.37534070279581044
1113	19	-0.8125755840147164
1113	20	-0.5690938504847125
1113	21	-0.2033023378593839
1113	22	-0.7559854507038466
1113	23	-4.256804187258158
1113	24	-1.8203718890027787
1113	25	-0.8643561520010508
1114	1	-0.5822087356115979
1114	2	-0.7942282116434143
1114	3	-0.6653477413275475
1114	4	-0.49663440572768636
1114	5	-0.5649067230909779
1114	6	-1.130108080099557
1114	7	-1.0555587737172267
1114	8	-0.868149881566751
1114	9	-0.9850091029087409
1114	10	-0.7919741472100341
1114	11	-0.9375026646578775
1114	12	-1.8799890611307006
1114	13	-1.0386043980708024
1114	14	-2.970295228806382
1114	15	-1.7152804463039786
1114	16	-1.6667007561424967
1114	17	-1.4710643768503644
1114	18	-1.4177589921506595
1114	19	-1.4669820232973905
1114	20	-1.1642433618464878
1114	21	-1.0853500233271802
1114	22	-1.1082800507803299
1114	23	-5.3474846263512354
1114	24	-2.745196617883522
1114	25	-2.6286091314991076
1115	1	-1.6550407865920427
1115	2	-1.488335154338845
1115	3	-1.2328959555885497
1115	4	-1.2720619239597006
1115	5	-1.2544221785404162
1115	6	-1.3844522687873067
1115	7	-1.954762278375803
1115	8	-2.1186735144869786
1115	9	-3.419029439832798
1115	10	-4.226883473955002
1115	11	-2.931423988255606
1115	12	-3.1938908108900144
1115	13	-2.4815370587289927
1115	14	-3.2734551520826685
1115	15	-2.901499287797648
1115	16	-3.016746645068068
1115	17	-2.8625332376188624
1115	18	-5.216031571874328
1115	19	-3.334273882300515
1115	20	-3.5569916513287545
1115	21	-3.224298143882848
1115	22	-2.614621099647465
1115	23	-3.248636433440815
1115	24	-2.9416632447484865
1115	25	-3.1432673319020026
1116	1	-1.710736349382124
1116	2	-1.582224630462246
1116	3	-1.8976336662795426
1116	4	-1.6739798169698865
1116	5	-1.685951316279759
1116	6	-3.748155701597913
1116	7	-2.468253579060928
1116	8	-2.8941285134253896
1116	9	-4.130753553064292
1116	10	-3.220698225121037
1116	11	-2.875039748729371
1116	12	-2.578248196232792
1116	13	-3.8710953695939487
1116	14	-6.65213040144172
1116	15	-4.75034997600352
1116	16	-3.652414321194666
1116	17	-4.717926675862973
1116	18	-3.37815547860982
1116	19	-3.0781093230193797
1116	20	-3.8880283491733856
1116	21	-4.6361918258356205
1116	22	-4.15202795385375
1116	23	-3.057362873034169
1116	24	-4.160943682077118
1116	25	-4.147700817700695
1201	1	0.7592359494837737
1201	2	0.7092404776545251
1201	3	1.043700594935732
1201	4	0.8733124338818641
1201	5	0.5605496915969681
1201	6	0.9477007604020642
1201	7	0.9952021438966341
1201	8	0.9822958808480422
1201	9	1.461496103802702
1201	10	1.2965076601534804
1201	11	1.4174989546870975
1201	12	1.276402764006967
1201	13	1.3153238910008511
1201	14	1.3605221153132732
1201	15	1.4559386075647325
1201	16	1.3112609435439282
1201	17	1.654510839666905
1201	18	1.4505269280748436
1201	19	1.5968111308749755
1201	20	1.555478439922254
1201	21	1.740578868604544
1201	22	1.3865933323990731
1201	23	1.8399973134021508
1201	24	1.6499484263100115
1201	25	1.7163137794523777
1202	1	1.1157142079889866
1202	2	1.1464175347521284
1202	3	1.1955650229725272
1202	4	1.3590272549983808
1202	5	1.1294309906829412
1202	6	1.4740216939363933
1202	7	1.3656658785140579
1202	8	1.3775239807447193
1202	9	1.6289038009831174
1202	10	1.4845140133133299
1202	11	1.4416411335838148
1202	12	1.5709739673595209
1202	13	1.6113448621113164
1202	14	2.0556204134130596
1202	15	1.8152386282879505
1202	16	1.4941352388688642
1202	17	1.9014346914023434
1202	18	1.8990603563855046
1202	19	1.9171931484147535
1202	20	1.7392198768984457
1202	21	1.8998264933957856
1202	22	1.8588407577967274
1202	23	1.9251985123112192
1202	24	1.951858367035733
1202	25	2.258420487846358
1203	1	1.00686097275835
1203	2	1.0923485163058402
1203	3	1.0294725545000318
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2211	16	-0.4086777517085949
2211	17	0.013977248075377702
2211	18	-0.3114400756592204
2211	19	-0.31979538372566907
2211	20	-0.3497904494052193
2211	21	-0.15237103747429614
2211	22	-0.033116788999386415
2211	23	0.21081855337244093
2211	24	-0.2657859033173935
2211	25	0.2620679388778129
2212	1	-0.019936053062114212
2212	2	0.14310808586423818
2212	3	-0.3257643090376128
2212	4	0.10933666388573471
2212	5	-0.21511860331164456
2212	6	-0.30554965272918366
2212	7	-0.14736024754857624
2212	8	-0.06043923971726883
2212	9	-0.011831289770469766
2212	10	-0.243303190916059
2212	11	-0.3407502506822624
2212	12	-0.08821863139893793
2212	13	0.01886196233931514
2212	14	-0.08392299478476417
2212	15	-0.3528130171625605
2212	16	-0.3837643236014969
2212	17	-0.1349772226612771
2212	18	0.021984495374020696
2212	19	-0.24271469797557899
2212	20	-0.0648903683810147
2212	21	0.0793673396546879
2212	22	0.013681274717903591
2212	23	0.06221902972764326
2212	24	0.0832660715194109
2212	25	0.2359461405693466
2213	1	-0.40986926178595695
2213	2	-0.18403895256079394
2213	3	-0.3877548968529041
2213	4	-0.2578255238429108
2213	5	-0.27995857571843885
2213	6	-0.18104645598904767
2213	7	-0.28337777500000527
2213	8	-0.21429194609225277
2213	9	-0.7946037448129744
2213	10	-0.4144221638499843
2213	11	-0.41567957097637986
2213	12	-0.43243646899505706
2213	13	-0.4194218246983823
2213	14	-0.579196507349991
2213	15	-0.36825441785257595
2213	16	-0.3853295734511164
2213	17	-0.4177755347155454
2213	18	-0.4285010702542138
2213	19	-0.4734940480662715
2213	20	-0.15487021269507295
2213	21	-0.34372780624626387
2213	22	-0.5585864003218646
2213	23	-0.45982572881784023
2213	24	-0.13241592140192182
2213	25	-0.014116729737928324
2214	1	-2.8213177919438515
2214	2	-1.3994076441514913
2214	3	-1.7147604480337506
2214	4	-3.4222216801726084
2214	5	-1.8826611458541862
2214	6	-1.6582952554747834
2214	7	-1.3540848292731589
2214	8	-1.035646589924621
2214	9	-0.9357999012390863
2214	10	-1.0993467344596013
2214	11	-1.0981505080825187
2214	12	-1.1008026439829521
2214	13	-1.582614514137159
2214	14	-1.4469556703174362
2214	15	-1.713647065496609
2214	16	-1.6007849156220857
2214	17	-3.8353919230759743
2214	18	-3.426401390511863
2214	19	-2.2705997258606097
2214	20	-2.17142547491612
2214	21	-1.9783600305726878
2214	22	-2.9742262756896487
2214	23	-1.828180298171688
2214	24	-3.0778772146238715
2214	25	-2.9021741225509174
2215	1	-1.8848669994008809
2215	2	-3.548872320890009
2215	3	-2.082974702984952
2215	4	-3.726009778150459
2215	5	-2.3189505242249595
2215	6	-2.1577742024286017
2215	7	-2.3112279861588974
2215	8	-1.6127595572680349
2215	9	-4.1640605840842255
2215	10	-2.4215410174898757
2215	11	-2.0086755219416865
2215	12	-1.9466838279371892
2215	13	-2.016671886046943
2215	14	-1.7229791809572745
2215	15	-1.965481456887261
2215	16	-1.9210547441659998
2215	17	-2.264638894980461
2215	18	-1.8877339537689153
2215	19	-2.1390473595693393
2215	20	-2.027882139965577
2215	21	-2.66933416234113
2215	22	-2.496362103598951
2215	23	-2.4813136349897498
2215	24	-2.280595561801647
2215	25	-3.243822279656296
2216	1	-3.317057976572926
2216	2	-3.4801754489933714
2216	3	-4.788315484551752
2216	4	-5.236054031041995
2216	5	-4.9263844988368
2216	6	-4.008150666441306
2216	7	-5.517105257108401
2216	8	-3.473845900346152
2216	9	-4.122358682242311
2216	10	-3.178042096488298
2216	11	-3.4673604587739533
2216	12	-3.854845575803658
2216	13	-3.3847017865947997
2216	14	-4.557095439716466
2216	15	-4.022194501821822
2216	16	-4.168317262784065
2216	17	-3.922593939907621
2216	18	-3.547824665638842
2216	19	-4.200407311223753
2216	20	-4.678593754306622
2216	21	-4.4721352873109055
2216	22	-4.169638354435062
2216	23	-4.0458776251282735
2216	24	-4.459549627777768
2216	25	-4.513846906445924
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	144203.0
3	2.0
4	1332.0
5	7580.0
6	8855.0
7	8139.0
8	7063.0
9	6650.0
10	5869.0
11	5563.0
12	5183.0
13	5160.0
14	5061.0
15	5177.0
16	5274.0
17	5837.0
18	6428.0
19	7839.0
20	10119.0
21	12885.0
22	16186.0
23	19081.0
24	22371.0
25	25429.0
26	28423.0
27	30838.0
28	35180.0
29	41334.0
30	50189.0
31	62482.0
32	77580.0
33	98988.0
34	127927.0
35	168061.0
36	228365.0
37	352557.0
38	972994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3631685506679	18.411821586402898	12.619880986683226	24.60512887624597
2	14.48662861344759	19.25229467318499	43.043248096258424	23.217828617109
3	19.96163922334111	24.599334311994628	28.160120582055566	27.2789058826087
4	12.277963709869656	15.65349886057	37.475099402156815	34.59343802740353
5	13.091954834022088	38.81707244978327	34.63004670303999	13.460926013154658
6	32.59329600068396	35.73258963194567	18.026349135588354	13.647765231782014
7	29.61503010410007	30.944915698978082	20.931919887789473	18.50813430913237
8	25.68423439656963	37.83656254199397	19.746325909818342	16.732877151618066
9	27.924671902386	14.78042345813969	19.82895106475233	37.46595357472198
10	16.783414314250237	28.4036319454544	33.01097469875977	21.801979041535596
11	34.92170851513175	21.71385886959333	24.78631728290779	18.578115332367133
12	25.262387587713842	23.89118483064959	30.858551579288118	19.98787600234845
13	30.04868404228317	22.00125574675901	25.37001589721961	22.580044313738213
14	21.038118918077114	22.777986864916898	26.682860953320297	29.501033263685688
15	23.0023260234465	32.6501705012592	22.322343947644242	22.02515952765006
16	21.754189223387204	27.53789586305006	28.93189260411844	21.776022309444297
17	21.51646541087942	29.097227312151087	27.86732235130282	21.51898492566668
18	21.429414008055716	26.672232599492958	31.123986307347145	20.77436708510418
19	24.17956300619429	26.134312285779398	27.126688809396292	22.559435898630017
20	24.709650649370975	26.662516046752117	28.087074458074984	20.540758845801925
21	23.17554827825742	26.365256753765237	26.51159151456859	23.947603453408753
22	24.004463460854243	28.753857618194672	26.51120904358555	20.730469877365536
23	22.188231369044598	28.174048607089702	28.037962192789212	21.599757831076488
24	23.976912441798238	26.09317015336484	28.775508957670265	21.154408447166656
25	22.71246245902365	27.401324679250816	28.44197504851508	21.444237813210453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	318.0
2	680.5
3	1043.0
4	1043.0
5	1043.0
6	1739.5
7	2436.0
8	2436.0
9	2436.0
10	2919.0
11	3402.0
12	3402.0
13	3402.0
14	5219.0
15	7036.0
16	7036.0
17	7036.0
18	14085.0
19	21134.0
20	21134.0
21	21134.0
22	37332.0
23	53530.0
24	53530.0
25	53530.0
26	84488.5
27	115447.0
28	115447.0
29	115447.0
30	155714.5
31	195982.0
32	195982.0
33	195982.0
34	223808.5
35	251635.0
36	251635.0
37	251635.0
38	269668.0
39	287701.0
40	287701.0
41	287701.0
42	304587.0
43	321473.0
44	321473.0
45	321473.0
46	339895.0
47	358317.0
48	358317.0
49	358317.0
50	343297.0
51	328277.0
52	328277.0
53	328277.0
54	288389.0
55	248501.0
56	248501.0
57	248501.0
58	215488.5
59	182476.0
60	182476.0
61	182476.0
62	152912.5
63	123349.0
64	123349.0
65	123349.0
66	95293.0
67	67237.0
68	67237.0
69	67237.0
70	49849.0
71	32461.0
72	32461.0
73	32461.0
74	22960.5
75	13460.0
76	13460.0
77	13460.0
78	9232.0
79	5004.0
80	5004.0
81	5004.0
82	3225.0
83	1446.0
84	1446.0
85	1446.0
86	944.0
87	442.0
88	442.0
89	442.0
90	257.5
91	73.0
92	73.0
93	73.0
94	46.0
95	19.0
96	19.0
97	19.0
98	12.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33876082867694507
2	0.009991594856845617
3	0.009724643849219968
4	0.010639904446793615
5	0.01216533877608302
6	0.08222091034869904
7	0.08119124217642869
8	0.10601768588561378
9	0.08588195273899361
10	0.0934709885272084
11	0.08828451180762442
12	0.09938204655320486
13	0.0857675451642969
14	0.08961926684575267
15	0.08702602848596067
16	0.08874214210641125
17	0.10113629603188767
18	0.10285240965233827
19	0.0965981289022517
20	0.09591168345407146
21	0.08328871437920161
22	0.10342444752582179
23	0.09705575920103852
24	0.09297522237018935
25	0.09152605975736441
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2622204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.671550821964374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82607851501318	38.663795187968
2	13.559861897063612	14.013181863058813
3	4.9800056667461154	7.719738477088272
4	2.2724990016198006	4.696941906202432
5	1.2291393515263473	3.1755768234834996
6	0.7373362313677131	2.285958393119548
7	0.48527496898251976	1.7552437155685214
8	0.3532519555034829	1.460246109740521
9	0.23373357290684577	1.0869638572129856
>10	1.1916226868960826	10.936579586114085
>50	0.07462710857174633	2.688271141733284
>100	0.04735206636982761	4.650781748601488
>500	0.0052774628848958295	1.8361570597975485
>1k	0.0034935317688675853	3.204815558043476
>5k	3.7165231583697716E-4	1.3898595503333964
>10k+	7.433046316739544E-5	0.43588902193422263
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	11349	0.432803855077637	No Hit
CTGTAGGACGTGGAATATGGCAAGA	9250	0.3527566886481754	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7919	0.3019978613410703	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6403	0.2441839002610018	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6387	0.24357372652928605	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6228	0.23751012507036065	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4289	0.16356469595805667	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3682	0.1404162300110899	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3602	0.1373653613525111	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3494	0.1332466886634297	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2927	0.11162365704575235	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.813585823223517E-5	0.0
4	0.0	0.0	0.0	3.813585823223517E-5	0.0
5	0.0	0.0	0.0	3.813585823223517E-5	0.0
6	0.0	0.0	0.0	3.813585823223517E-5	3.813585823223517E-5
7	0.0	0.0	0.0	7.627171646447034E-5	3.813585823223517E-5
8	0.0	0.0	0.0	7.627171646447034E-5	3.813585823223517E-5
9	0.0	0.0	0.0	7.627171646447034E-5	3.813585823223517E-5
10	0.0	0.0	0.0	7.627171646447034E-5	7.627171646447034E-5
11	0.0	0.0	0.0	1.5254343292894068E-4	7.627171646447034E-5
12	0.0	0.0	0.0	1.5254343292894068E-4	2.2881514939341105E-4
13	3.813585823223517E-5	0.0	0.0	1.5254343292894068E-4	2.2881514939341105E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGGT	45	6.7703973E-4	14.776085	17
TAGGACC	1550	0.0	13.910779	4
TGGACCG	110	3.824971E-8	12.0892935	5
GGTATCA	1970	0.0	12.037488	1
TAGAAAT	1015	0.0	11.884884	4
TGTAGGA	4870	0.0	11.663516	2
GGACCTG	2845	0.0	11.552059	6
AGGACCT	2930	0.0	11.541121	5
GTGTAGG	460	0.0	11.386954	1
GTCCTAC	4985	0.0	11.386332	1
GGCGAGG	1900	0.0	11.348267	19
TCCAACG	245	0.0	11.24361	18
CTATAGT	355	0.0	11.2377205	4
AATGTCC	1035	0.0	11.196571	8
GTATCAA	4440	0.0	11.175256	1
TACCGTA	60	0.005883329	11.081853	12
CTGTAGG	4865	0.0	11.079912	1
TTAGAAA	980	0.0	11.049334	3
GTAGGAC	4820	0.0	10.97654	3
TCCCGCC	355	0.0	10.970574	13
>>END_MODULE