FastQCFastQC Report
Thu 26 May 2016
SRR1512903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622204
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT105170.40107482102841735No Hit
CTGTAGGACGTGGAATATGGCAAGA90220.34406171297122573No Hit
GTATCAACGCAGAGTACTTTTTTTT82130.3132098036613475No Hit
GTCCTAAAGTGTGTATTTCTCATTT73980.2821290792020758No Hit
CTTTAGGACGTGAAATATGGCGAGG69690.2657687960204469No Hit
TATCAACGCAGAGTACTTTTTTTTT58090.2215312004710541No Hit
GTCCTACAGTGTGCATTTCTCATTT57520.2193574565518167No Hit
GGTATCAACGCAGAGTACTTTTTTT46140.1759588498835331No Hit
CTGTAGGACCTGGAATATGGCGAGA42130.1606663707324068No Hit
CTGAAGGACCTGGAATATGGCGAGA38470.14670864661940872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30780.11738217163881985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC350.002181535116.27361912
TAGGACC15350.014.3739584
GGTATCA19900.012.9517831
AGGACCG1600.012.4823435
TCCAACG2800.012.20404918
CGGTTTG550.003086540612.08192814
GGCGAGG18200.011.8385219
CCAACGA2250.011.81186419
CGTAAAA752.0792487E-411.3974019
CACCTTT9100.011.2663514
AGGACCT29800.011.2337095
TGTAGGA48950.011.1343552
ACGGTGC600.00587326511.0844028
TAGAAAT9900.011.0473834
TTTAGAA10800.011.0090392
GGACCTG29300.010.8734076
CTGTAGG49500.010.7596011
GTCCTAC48700.010.7215291
TAGGACA11800.010.7192994
GTAGGAC48050.010.6896513
Produced by FastQC (version 0.11.5)