##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512903_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2622204 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.073287204199215 31.0 30.0 34.0 26.0 34.0 2 30.10682006434282 31.0 30.0 34.0 26.0 34.0 3 30.281199326978374 31.0 30.0 34.0 26.0 34.0 4 33.850530317244576 37.0 35.0 37.0 30.0 37.0 5 33.408680636594255 37.0 35.0 37.0 28.0 37.0 6 33.27688730548805 37.0 35.0 37.0 27.0 37.0 7 33.108232997890326 37.0 35.0 37.0 26.0 37.0 8 33.05446906495452 37.0 35.0 37.0 26.0 37.0 9 34.38046772867405 39.0 35.0 39.0 26.0 39.0 10 34.2872758946291 39.0 35.0 39.0 25.0 39.0 11 34.26499768896699 39.0 35.0 39.0 25.0 39.0 12 34.13001620011258 39.0 35.0 39.0 25.0 39.0 13 34.114644017017746 39.0 35.0 39.0 25.0 39.0 14 35.17421527844515 39.0 35.0 41.0 25.0 41.0 15 35.18308186548415 40.0 35.0 41.0 25.0 41.0 16 35.181496176498854 40.0 35.0 41.0 25.0 41.0 17 35.17218797622153 40.0 35.0 41.0 25.0 41.0 18 35.08915782296114 40.0 35.0 41.0 24.0 41.0 19 35.09992471981585 40.0 35.0 41.0 24.0 41.0 20 35.072661394765625 40.0 35.0 41.0 24.0 41.0 21 35.03444659530685 40.0 35.0 41.0 23.0 41.0 22 34.9952646704833 40.0 35.0 41.0 23.0 41.0 23 34.82724036726357 40.0 35.0 41.0 21.0 41.0 24 34.78855420859704 40.0 35.0 41.0 19.0 41.0 25 34.72560334741309 40.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6506942548981769 1101 2 0.5289850577921591 1101 3 0.4601568580166919 1101 4 0.5247511229463342 1101 5 0.6447963657120326 1101 6 0.7309640966047937 1101 7 0.7731189475605689 1101 8 0.4632797517829772 1101 9 0.7936780256542733 1101 10 0.47548735984875634 1101 11 0.8124621759329358 1101 12 0.8791503824694473 1101 13 1.0130296199515527 1101 14 1.0908215001385102 1101 15 0.7117788840721886 1101 16 0.9763658762242642 1101 17 0.9386628041252791 1101 18 0.8933961458669017 1101 19 0.8424691137784563 1101 20 0.9246421177348125 1101 21 0.9637375157292425 1101 22 0.8531163103624095 1101 23 1.0516365618139787 1101 24 1.079011299632839 1101 25 1.098904529026541 1102 1 0.9334190720960969 1102 2 1.0277423745110568 1102 3 0.7376970806550958 1102 4 0.7950345820623355 1102 5 1.0025995266582157 1102 6 0.9313924375716169 1102 7 1.0162157353123646 1102 8 1.0800032833398987 1102 9 1.1409061713025679 1102 10 1.0255887276966078 1102 11 1.1463969172903958 1102 12 1.1336223116108428 1102 13 1.1288729819027594 1102 14 1.0624218141315964 1102 15 1.2175432532736679 1102 16 1.123951281726562 1102 17 1.2853352809002985 1102 18 0.6536825100370791 1102 19 1.050797965911201 1102 20 1.1797751012254523 1102 21 1.0751893011013678 1102 22 0.9534828416659096 1102 23 1.1563198800039487 1102 24 1.0944229210857372 1102 25 1.2207916398574454 1103 1 0.6823420975932741 1103 2 0.9195128903786909 1103 3 0.6459042604058105 1103 4 0.5228326816100619 1103 5 0.6728094971331586 1103 6 0.6718977299552975 1103 7 0.7788516730837358 1103 8 0.8357142161745656 1103 9 0.8945491608272391 1103 10 0.8630635386149308 1103 11 1.0029798027517458 1103 12 1.1235406121478135 1103 13 1.171229954311947 1103 14 1.1855553239966738 1103 15 1.2403669404080233 1103 16 1.2570400400802981 1103 17 1.1799356577551308 1103 18 1.1950686768000978 1103 19 1.2400987027290142 1103 20 1.1170730830060478 1103 21 1.2656661628332344 1103 22 1.1591335824092965 1103 23 1.2716612500520768 1103 24 1.2888904827826337 1103 25 1.302743112660373 1104 1 0.9256977403592579 1104 2 1.023043104357921 1104 3 0.9397514534304285 1104 4 0.8169270205283397 1104 5 0.9277667059536512 1104 6 0.9076115073618354 1104 7 0.9346206660444238 1104 8 0.9574311480889932 1104 9 1.0760440172527979 1104 10 1.1543257617496963 1104 11 1.142479572929986 1104 12 1.158879850072843 1104 13 1.1237627184961454 1104 14 1.3299235241306064 1104 15 1.37714128109954 1104 16 1.2169511912190245 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1.0973167846123602 1107 17 1.1223751364333552 1107 18 1.1685246197606745 1107 19 1.192168056818339 1107 20 1.2268207788554264 1107 21 1.1481260701104006 1107 22 1.1208547192544387 1107 23 1.3032333850049724 1107 24 1.3042680695608198 1107 25 1.3287869519955606 1108 1 0.6596758389010517 1108 2 0.6148169952572644 1108 3 0.6255187427700228 1108 4 0.45078193614604345 1108 5 0.7906253221704063 1108 6 0.745782539099757 1108 7 0.7269892879631499 1108 8 0.6398277459913047 1108 9 0.7716199953899618 1108 10 0.7251312951561815 1108 11 0.8443005107331132 1108 12 0.8672079017040346 1108 13 0.8504204346654234 1108 14 0.9724483254136587 1108 15 0.944178405218409 1108 16 0.9003009943762308 1108 17 0.9078827267137441 1108 18 1.0073325889107494 1108 19 1.0881227586506554 1108 20 1.0168238361195847 1108 21 1.0914178193187567 1108 22 1.0221434138648533 1108 23 1.1134181628511826 1108 24 1.081824328998124 1108 25 1.032991867791928 1109 1 0.31216637091942445 1109 2 0.2427098366125442 1109 3 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-4.038804114888322 2216 25 -4.133332444593275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84707.0 3 2.0 4 1432.0 5 12031.0 6 14085.0 7 10637.0 8 8332.0 9 7222.0 10 6312.0 11 5640.0 12 5026.0 13 4601.0 14 4591.0 15 4536.0 16 4591.0 17 4820.0 18 5346.0 19 6412.0 20 8446.0 21 11000.0 22 13855.0 23 16599.0 24 19726.0 25 22361.0 26 25099.0 27 27264.0 28 30823.0 29 36535.0 30 45691.0 31 57880.0 32 73292.0 33 94649.0 34 123422.0 35 162460.0 36 222076.0 37 345954.0 38 1094749.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.99900762411855 19.376253590206012 13.675054151280069 26.949684634395364 2 13.018090558473553 19.70713721881461 41.81121525349559 25.463556969216256 3 17.903354323362404 24.648923359884595 28.9925587047592 28.4551636119938 4 11.632903141901387 16.301876689304507 36.65007507596268 35.415145092831416 5 12.66972606193578 39.02445443729924 34.8185170337318 13.48730246703318 6 32.71793282821191 36.29830147323691 17.494383017174535 13.48938268137665 7 29.250144937603363 31.181412262847157 20.866892892733198 18.701549906816282 8 25.678346227594034 38.13471720072119 19.363789863298578 16.823146708386197 9 27.19865606532914 14.634667350230169 19.78431893119964 38.38235765324105 10 16.164413877309624 28.96713821029638 32.91887096620339 21.949576946190607 11 34.711418324055415 21.797706785212487 24.699122348012736 18.791752542719365 12 24.81951189747521 24.180238311796035 30.895337684649693 20.104912106079066 13 29.78658244735117 21.877368143848138 25.562166600624014 22.773882808176676 14 20.80390331559873 22.717577467001707 26.760318986370507 29.71820023102906 15 22.85357826270865 32.66128032581984 22.34744072526801 22.137700686203498 16 21.681833437581048 27.637073029336833 28.681177439777876 21.999916093304243 17 21.234746254949826 29.251378972495303 27.895079481369805 21.618795291185062 18 21.247282186897063 26.712541661057195 31.18361833265765 20.85655781938809 19 23.965898278324506 26.116953421899165 27.39544382387769 22.52170447589864 20 24.529778412367172 26.619804680706956 28.186027744980493 20.664389161945383 21 22.81806428977305 26.229640109984402 26.899461900167417 24.052833700075126 22 23.796682847803876 28.441428135042894 26.80005140743097 20.96183760972226 23 21.784003075275606 28.062233144332023 28.28647199073756 21.86729178965481 24 23.88570835831232 26.0510242528804 28.87101842572126 21.192248963086012 25 22.38784625452482 27.3302534814225 28.622601445196484 21.6592988188562 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90.0 1 90.0 2 271.5 3 453.0 4 453.0 5 453.0 6 1210.0 7 1967.0 8 1967.0 9 1967.0 10 2613.5 11 3260.0 12 3260.0 13 3260.0 14 5118.5 15 6977.0 16 6977.0 17 6977.0 18 14593.0 19 22209.0 20 22209.0 21 22209.0 22 39249.0 23 56289.0 24 56289.0 25 56289.0 26 88028.0 27 119767.0 28 119767.0 29 119767.0 30 159065.5 31 198364.0 32 198364.0 33 198364.0 34 225017.5 35 251671.0 36 251671.0 37 251671.0 38 270584.5 39 289498.0 40 289498.0 41 289498.0 42 305939.0 43 322380.0 44 322380.0 45 322380.0 46 340176.0 47 357972.0 48 357972.0 49 357972.0 50 342279.0 51 326586.0 52 326586.0 53 326586.0 54 286395.0 55 246204.0 56 246204.0 57 246204.0 58 213680.0 59 181156.0 60 181156.0 61 181156.0 62 151183.5 63 121211.0 64 121211.0 65 121211.0 66 93020.0 67 64829.0 68 64829.0 69 64829.0 70 48291.0 71 31753.0 72 31753.0 73 31753.0 74 22433.5 75 13114.0 76 13114.0 77 13114.0 78 8870.0 79 4626.0 80 4626.0 81 4626.0 82 2994.0 83 1362.0 84 1362.0 85 1362.0 86 889.0 87 416.0 88 416.0 89 416.0 90 229.5 91 43.0 92 43.0 93 43.0 94 25.0 95 7.0 96 7.0 97 7.0 98 3.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0850048279996522 2 0.04244521021247775 3 0.01841961952616959 4 0.007360220638821388 5 0.021089129602426048 6 0.06025465600693157 7 0.08050479672824845 8 0.058309727237087584 9 0.02471203613448839 10 0.0525893485022523 11 0.007932258512304917 12 3.4322272409011655E-4 13 0.006139873175389863 14 8.77124739341409E-4 15 0.014758577135875011 16 0.009305149408665381 17 0.00545342772720963 18 0.005758514593067511 19 0.0018686570533795234 20 0.0013728908963604662 21 5.339020152512925E-4 22 5.720378734835275E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2622204.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.258698472234784 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31063833166995 36.84497266718457 2 14.447042416478403 14.521790972507487 3 5.188978702182561 7.823739479155245 4 2.4384032514401293 4.902038950713854 5 1.3004690310159268 3.26799404511544 6 0.7666069758217611 2.3117201306722603 7 0.5233448105704613 1.8411840322028754 8 0.3627027115941224 1.4583172973656746 9 0.26665470779356537 1.206154669967881 >10 1.2570411665665817 11.226753960619613 >50 0.07851746512691428 2.7189406472616318 >100 0.050327171363951914 4.854483043269528 >500 0.005016680760628871 1.6929125365602624 >1k 0.0037245054131836083 3.2783252225463126 >5k 4.560618873286051E-4 1.6489040689471255 >10k+ 7.601031455476751E-5 0.4017682759103147 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 10517 0.40107482102841735 No Hit CTGTAGGACGTGGAATATGGCAAGA 9022 0.34406171297122573 No Hit GTATCAACGCAGAGTACTTTTTTTT 8213 0.3132098036613475 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7398 0.2821290792020758 No Hit CTTTAGGACGTGAAATATGGCGAGG 6969 0.2657687960204469 No Hit TATCAACGCAGAGTACTTTTTTTTT 5809 0.2215312004710541 No Hit GTCCTACAGTGTGCATTTCTCATTT 5752 0.2193574565518167 No Hit GGTATCAACGCAGAGTACTTTTTTT 4614 0.1759588498835331 No Hit CTGTAGGACCTGGAATATGGCGAGA 4213 0.1606663707324068 No Hit CTGAAGGACCTGGAATATGGCGAGA 3847 0.14670864661940872 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3078 0.11738217163881985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.1440757469670553E-4 7 0.0 0.0 0.0 0.0 1.1440757469670553E-4 8 0.0 0.0 0.0 0.0 1.1440757469670553E-4 9 0.0 0.0 0.0 3.813585823223517E-5 1.5254343292894068E-4 10 0.0 0.0 0.0 3.813585823223517E-5 1.5254343292894068E-4 11 0.0 0.0 0.0 7.627171646447034E-5 1.9067929116117587E-4 12 0.0 0.0 0.0 7.627171646447034E-5 3.8135858232235173E-4 13 0.0 0.0 0.0 7.627171646447034E-5 4.576302987868221E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACC 35 0.0021815351 16.273619 12 TAGGACC 1535 0.0 14.373958 4 GGTATCA 1990 0.0 12.951783 1 AGGACCG 160 0.0 12.482343 5 TCCAACG 280 0.0 12.204049 18 CGGTTTG 55 0.0030865406 12.081928 14 GGCGAGG 1820 0.0 11.83852 19 CCAACGA 225 0.0 11.811864 19 CGTAAAA 75 2.0792487E-4 11.397401 9 CACCTTT 910 0.0 11.26635 14 AGGACCT 2980 0.0 11.233709 5 TGTAGGA 4895 0.0 11.134355 2 ACGGTGC 60 0.005873265 11.084402 8 TAGAAAT 990 0.0 11.047383 4 TTTAGAA 1080 0.0 11.009039 2 GGACCTG 2930 0.0 10.873407 6 CTGTAGG 4950 0.0 10.759601 1 GTCCTAC 4870 0.0 10.721529 1 TAGGACA 1180 0.0 10.719299 4 GTAGGAC 4805 0.0 10.689651 3 >>END_MODULE