FastQCFastQC Report
Thu 26 May 2016
SRR1512902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115735
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41970.19837077894915953No Hit
GTCCTACAGTGGACATTTCTAAATT41430.19581847443087153No Hit
GTCCTAAAGTGTGTATTTCTCATTT36830.17407662112693698No Hit
CTGTAGGACGTGGAATATGGCAAGA33230.15706125767168386No Hit
CTTTAGGACGTGAAATATGGCGAGG26500.12525198099005783No Hit
GTACTGGTTCACTATCGGTCAGTCA24560.11608259068361586No Hit
TATCAACGCAGAGTACTTTTTTTTT23970.11329396167289382No Hit
GGTATCAACGCAGAGTACTTTTTTT23020.10880379631664645No Hit
GTCCTACAGTGTGCATTTCTCATTT22380.10577884281349034No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTACG400.005202985414.2815671
TCGTGCA500.00150161913.2979319
CCGAATG1155.335096E-912.39025618
TAGGACC6550.011.74626354
GACGCTC907.4769505E-611.6093038
GTCCTAG2800.011.5612681
GGTATCA15100.011.3495891
TCTATAC1354.7421054E-911.2575073
TGGCGAG18700.010.97233318
GTACTAG1053.3768029E-610.8811941
GTATCAA31950.010.7577371
CTAGCAC1252.2458335E-710.6383443
CTGTAGG19900.010.5257771
GGCGAGG8950.010.40110819
TGTAGGA20600.010.37459852
TTTATAC1659.313226E-1010.3620233
ATGGCGA21000.010.35864617
CGTTTCC1201.5258574E-610.29079514
GGCGAGA11800.010.22346919
TAGGACG22100.010.1861974
Produced by FastQC (version 0.11.5)