##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512902_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115735 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.16816614557116 31.0 28.0 33.0 16.0 34.0 2 28.338297565621403 31.0 28.0 34.0 16.0 34.0 3 28.44961018274973 31.0 28.0 34.0 16.0 34.0 4 31.83808180135981 35.0 32.0 37.0 17.0 37.0 5 31.975023337043627 35.0 33.0 37.0 19.0 37.0 6 31.902728838914136 35.0 32.0 37.0 17.0 37.0 7 31.826622426721684 35.0 32.0 37.0 17.0 37.0 8 31.759678787749884 35.0 32.0 37.0 17.0 37.0 9 32.9837200783652 38.0 33.0 39.0 17.0 39.0 10 32.85269658062092 37.0 33.0 39.0 15.0 39.0 11 32.89567455281498 38.0 33.0 39.0 15.0 39.0 12 32.77546101000362 38.0 33.0 39.0 11.0 39.0 13 32.73463217274375 38.0 33.0 39.0 10.0 39.0 14 33.53833915873207 38.0 33.0 41.0 10.0 41.0 15 33.68370613522015 39.0 33.0 41.0 10.0 41.0 16 33.608266158096356 39.0 33.0 41.0 10.0 41.0 17 33.36616920361009 38.0 32.0 41.0 10.0 41.0 18 33.34093683755291 38.0 32.0 41.0 8.0 41.0 19 33.44189844191262 39.0 33.0 41.0 2.0 41.0 20 33.40362852625683 39.0 33.0 41.0 2.0 41.0 21 33.30214606271579 39.0 33.0 41.0 2.0 41.0 22 33.28240918640567 39.0 33.0 41.0 2.0 41.0 23 32.92314302121957 39.0 32.0 41.0 2.0 41.0 24 32.97024438315763 39.0 32.0 41.0 2.0 41.0 25 32.666000231598005 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7817651586236032 1101 2 0.3885765662485561 1101 3 0.7718648811437632 1101 4 0.8710964470660691 1101 5 0.6886670258868719 1101 6 0.7521228774475226 1101 7 0.5339232825406413 1101 8 0.7701760479145001 1101 9 0.9210411701956431 1101 10 0.9004510150353511 1101 11 0.8738599713861248 1101 12 0.6551016466982773 1101 13 1.1547337677336387 1101 14 1.2633750872508358 1101 15 0.9549644695995525 1101 16 0.8678121069634486 1101 17 0.30698908918816414 1101 18 0.7821560622099781 1101 19 0.12200969937258321 1101 20 -0.6155748885384327 1101 21 0.6665241511672519 1101 22 0.7435558769587871 1101 23 0.207572437129663 1101 24 0.65283920193189 1101 25 0.3818962023084822 1102 1 1.069922928154483 1102 2 1.169491337714284 1102 3 1.050353161030845 1102 4 1.131205242701558 1102 5 0.8942083079625576 1102 6 0.9053455120147156 1102 7 0.8992937057757544 1102 8 0.8682528286632767 1102 9 1.1779955267788864 1102 10 1.1570881831769455 1102 11 0.9989067566812722 1102 12 1.173742811414236 1102 13 0.9514270782294076 1102 14 1.1664338411231796 1102 15 1.1331186443280927 1102 16 1.1567094542107768 1102 17 1.2816753369546277 1102 18 1.3200072162472622 1102 19 1.031286403996134 1102 20 1.2994524425322922 1102 21 1.5265039568364642 1102 22 1.2475989722950658 1102 23 1.1268331766016502 1102 24 0.4625208504659568 1102 25 0.8499077479677553 1103 1 0.6313122157496132 1103 2 0.7938391518288306 1103 3 0.9267102824979894 1103 4 0.9802084197305696 1103 5 0.8470600615242319 1103 6 0.4178725752764869 1103 7 0.38030364302684916 1103 8 0.4504034673390471 1103 9 0.6501767310208919 1103 10 0.9268998676218985 1103 11 0.6567035576230182 1103 12 0.7825498593932281 1103 13 1.0380006025286903 1103 14 1.092301672585748 1103 15 1.0416276091949044 1103 16 1.0324573295941377 1103 17 0.6514278178253292 1103 18 1.0003259861897433 1103 19 1.0484824607627274 1103 20 1.1515627745886192 1103 21 0.9419486892672921 1103 22 1.008214482943103 1103 23 0.25592433289563843 1103 24 0.45527615727471016 1103 25 0.8847767600597436 1104 1 0.7615863214861207 1104 2 0.6812106473101629 1104 3 0.7421803494799519 1104 4 0.8409744148173637 1104 5 0.6058600970006012 1104 6 0.536884287556358 1104 7 0.6922595013502004 1104 8 0.3939037088694839 1104 9 0.7645797310760685 1104 10 0.6857715836457388 1104 11 0.4945235685299707 1104 12 0.8331526868734684 1104 13 0.8264375400648873 1104 14 0.9602581490374575 1104 15 0.6865137987653256 1104 16 0.6460032097144719 1104 17 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17 1.236301925738502 1107 18 1.152798211258414 1107 19 0.9000856552517007 1107 20 1.0812385656907253 1107 21 0.8903494614450906 1107 22 0.8558485314884905 1107 23 1.1839483378407536 1107 24 0.9128647213743903 1107 25 1.2938196033664866 1108 1 1.0932795462805416 1108 2 0.9870058781820532 1108 3 1.0656539758315517 1108 4 1.228707556147608 1108 5 1.0498035066850662 1108 6 1.3377222312308525 1108 7 1.1473721257505645 1108 8 1.2060438468133903 1108 9 1.2837241871182385 1108 10 1.258699410656341 1108 11 0.921864493279152 1108 12 1.1479534836665835 1108 13 1.2306479897772604 1108 14 1.3010842518642463 1108 15 1.2395020850654532 1108 16 1.1163196928381467 1108 17 1.4316472749375677 1108 18 1.525428697721317 1108 19 1.4579615226750846 1108 20 1.512488585742119 1108 21 1.4695697990143444 1108 22 1.6219461128089279 1108 23 1.890620479875217 1108 24 1.6510934291982835 1108 25 1.849679744079161 1109 1 0.7642136993302096 1109 2 0.755078358363253 1109 3 0.8198474720295792 1109 4 0.9196873328827628 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1.4306799538019064 2207 1 1.0589490623278692 2207 2 1.057825430962783 2207 3 1.042294778821148 2207 4 1.0937341712902544 2207 5 0.8801704296456947 2207 6 1.0955688579902763 2207 7 1.0918662258616934 2207 8 1.170760548710529 2207 9 1.296174424625562 2207 10 1.3649318893522917 2207 11 1.2669804753606755 2207 12 1.0902329705128366 2207 13 1.1153932827294852 2207 14 1.443710650584812 2207 15 1.364118174562357 2207 16 1.1826374142530582 2207 17 1.481703833773345 2207 18 1.4474527614173098 2207 19 1.4141953937925464 2207 20 1.418773976455597 2207 21 1.3681143206976643 2207 22 1.0327134431523177 2207 23 1.473742739788591 2207 24 1.5044465791209731 2207 25 1.552020139420783 2208 1 0.668430203323112 2208 2 0.9154137620899618 2208 3 0.9583656619142289 2208 4 0.9421966496529528 2208 5 0.1962648289164548 2208 6 0.6541314134472422 2208 7 0.7647016465588337 2208 8 0.905243507966933 2208 9 0.5751550831755097 2208 10 0.8087621655825288 2208 11 0.7505658196908556 2208 12 0.7732858067578121 2208 13 0.5999041219903489 2208 14 0.8563116852966317 2208 15 0.8144390370484729 2208 16 0.7569400504893053 2208 17 1.1196195488833354 2208 18 1.2556522707292288 2208 19 1.0832833782379296 2208 20 0.9293477711908835 2208 21 0.9108864901499061 2208 22 0.87586599283933 2208 23 1.3521034925002269 2208 24 1.0537778226661487 2208 25 1.1059455228314548 2209 1 0.3477806570872808 2209 2 0.34446297374786994 2209 3 0.2100906509039362 2209 4 0.06228007094318144 2209 5 -0.10326788140434573 2209 6 0.2643943290971116 2209 7 0.045197862746903184 2209 8 -0.012800731995923798 2209 9 -0.1284000191940322 2209 10 0.20746086196915314 2209 11 0.16073381888183036 2209 12 0.07343436676042359 2209 13 -0.09733720189528583 2209 14 0.22415595794216614 2209 15 0.14917976727370075 2209 16 0.2700301219007031 2209 17 0.2879932325251886 2209 18 0.33537681922075535 2209 19 0.26578475219952225 2209 20 0.16892021804670776 2209 21 0.09121348825080133 2209 22 0.15241346979773596 2209 23 0.49423985160279216 2209 24 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0.06428777404637742 2211 11 -0.13693355586074318 2211 12 -0.10700353554008757 2211 13 -0.0029459001726124256 2211 14 0.3904678896441638 2211 15 0.08068956336926902 2211 16 -0.21255118463395206 2211 17 0.4177078248744692 2211 18 0.04829390289594926 2211 19 0.11050589253019893 2211 20 0.022065605886638195 2211 21 0.1604599489385592 2211 22 0.12644702721134848 2211 23 0.3620658564332473 2211 24 -0.05456404149141747 2211 25 0.36716530778860346 2212 1 0.17231951943603008 2212 2 0.2461153954002917 2212 3 -0.16338004119793226 2212 4 0.2388407720362018 2212 5 -0.10398769334848978 2212 6 -0.10974331132189619 2212 7 0.046821729374570964 2212 8 0.10186541470064014 2212 9 0.27409254415024265 2212 10 -0.15021565163131356 2212 11 -0.294940506511125 2212 12 0.33327607919529356 2212 13 0.1624398609236053 2212 14 0.2849903799629061 2212 15 -0.14394592466567957 2212 16 -0.1610289922002366 2212 17 0.19233675943905837 2212 18 0.36416323066875833 2212 19 0.08323789531046089 2212 20 0.17218138386300552 2212 21 0.4562804235686855 2212 22 0.4511401063494702 2212 23 0.5930252447760154 2212 24 0.6257997754085949 2212 25 0.7266799538019058 2213 1 -0.4609387251098873 2213 2 -0.40364614696399315 2213 3 -0.6103612690010785 2213 4 -0.46365339336207256 2213 5 -0.46816019461688185 2213 6 -0.2600954168520815 2213 7 -0.45128481552649546 2213 8 -0.17890170858703058 2213 9 -0.7528135948654864 2213 10 -0.36408475361000114 2213 11 -0.39930986216815256 2213 12 -0.5069349811801516 2213 13 -0.6476058011768515 2213 14 -0.6655606093826094 2213 15 -0.5517064523166155 2213 16 -0.4230685660206319 2213 17 -0.46037151047469393 2213 18 -0.4954613253941531 2213 19 -0.2696712878452985 2213 20 -0.26303728686251304 2213 21 -0.41262267130701247 2213 22 -0.6931440133867071 2213 23 -0.5338098643056668 2213 24 -0.1966984488430512 2213 25 -0.13281877780641338 2214 1 -2.764005633215028 2214 2 -1.3017936976520232 2214 3 -1.363410689287143 2214 4 -3.239133436417955 2214 5 -1.6106092837485662 2214 6 -1.3301381530127259 2214 7 -1.128141988002895 2214 8 -0.9253237783115935 2214 9 -0.7895579733309575 2214 10 -0.8438731945975775 2214 11 -0.8560297867525364 2214 12 -0.7524327639572164 2214 13 -1.0989930551242075 2214 14 -0.992725441523767 2214 15 -1.4227914818715135 2214 16 -1.226869574985436 2214 17 -3.529514762356449 2214 18 -3.2177907028956128 2214 19 -2.0213043401140176 2214 20 -1.6784570865615898 2214 21 -1.6769354097057096 2214 22 -2.7756573569317595 2214 23 -1.6016675434928374 2214 24 -2.9910870388404085 2214 25 -2.663473616700486 2215 1 -1.9274395283556451 2215 2 -3.6748368129254914 2215 3 -2.341133651251706 2215 4 -3.8131618864134396 2215 5 -2.437303142566062 2215 6 -2.206901428656195 2215 7 -2.45644743865153 2215 8 -1.7921320476883373 2215 9 -4.407400537838761 2215 10 -2.695090039885798 2215 11 -2.035105572066076 2215 12 -1.960067887936603 2215 13 -1.9889830138271662 2215 14 -1.7700163265805067 2215 15 -1.9026660020023947 2215 16 -1.897980233817055 2215 17 -2.2329141619554207 2215 18 -1.973116691994523 2215 19 -2.0557328013241865 2215 20 -2.0548801834138963 2215 21 -2.619258697934548 2215 22 -2.447144226923445 2215 23 -2.3658383888412864 2215 24 -2.385188321987332 2215 25 -3.3375960416062256 2216 1 -3.923459770057544 2216 2 -3.834595111024502 2216 3 -5.288928038174504 2216 4 -6.019169809974379 2216 5 -5.840670988888931 2216 6 -4.693410733846459 2216 7 -6.633015005319308 2216 8 -4.075803519539722 2216 9 -4.8027449464015355 2216 10 -3.736827896529274 2216 11 -3.919608684893589 2216 12 -4.427773051568124 2216 13 -4.151619095992494 2216 14 -5.158039098495145 2216 15 -4.648713876290774 2216 16 -4.8680584706582835 2216 17 -4.578097102994803 2216 18 -4.1103341238285935 2216 19 -4.849499821998688 2216 20 -5.481838268442363 2216 21 -5.213881330021639 2216 22 -4.686843264776758 2216 23 -4.757946788481732 2216 24 -5.19115562897538 2216 25 -5.298804551111168 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 134414.0 3 2.0 4 1200.0 5 6980.0 6 8070.0 7 7356.0 8 6397.0 9 6064.0 10 5343.0 11 5272.0 12 4907.0 13 4822.0 14 4726.0 15 4882.0 16 5031.0 17 5322.0 18 6010.0 19 7277.0 20 9331.0 21 12038.0 22 15090.0 23 17659.0 24 20545.0 25 23105.0 26 25940.0 27 28623.0 28 31843.0 29 37302.0 30 44811.0 31 55582.0 32 68636.0 33 85790.0 34 109859.0 35 139861.0 36 185792.0 37 278913.0 38 700940.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.03863785323971 17.86461243107836 12.531858325658247 24.564891390023686 2 15.573564363736747 19.44100424106172 40.598995607674375 24.386435787527155 3 19.796826423247875 24.50009004831411 28.162149361082395 27.54093416735562 4 12.89170557014633 15.623457805560125 36.45227682260202 35.03255980169152 5 14.027109750765195 38.11612049303348 33.71725026294331 14.139519493258016 6 33.23005473450383 34.783774821749816 17.888925522570002 14.097244921176355 7 29.98155666653895 30.855663768428553 20.623279658928414 18.539499906104087 8 26.858267314178004 35.37615777978811 19.840144062439673 17.925430843594217 9 28.14596055424442 14.461141536116665 19.07844164051882 38.31445626912009 10 17.211579947317425 27.77676327661378 32.229992354971536 22.78166442109725 11 35.6750453546652 21.822275940857992 23.408778581717634 19.093900122759173 12 24.90156893580904 23.933296809045153 29.79866222697858 21.366472028167227 13 29.913773039741603 21.186959425834786 25.104448720786998 23.794818813636613 14 22.321739866774713 21.40701644335513 26.222749534856327 30.048494155013827 15 24.263777851364775 29.92099910118738 22.342542220540235 23.47268082690761 16 23.3460461692608 26.904489103891716 26.464112808994294 23.28535191785319 17 22.886696561467122 27.680525578222152 26.400928448350093 23.031849411960632 18 22.712061518575418 26.296749627289902 28.64438559728006 22.346803256854624 19 24.68628590136386 25.909491755720282 26.4047824959739 22.999439846941964 20 25.205452881023966 25.829511128352756 26.814208904101488 22.15082708652179 21 24.760179736884805 25.46491710655593 25.85167591541827 23.923227241140996 22 25.01625206166983 26.736841507623566 25.81873013439769 22.428176296308912 23 23.923111314019696 26.424359122961818 26.609957397082372 23.042572165936118 24 24.513967505898147 25.620553326025796 26.988753424521445 22.876725743554612 25 23.942973681434324 26.11854968538166 26.778914681448047 23.15956195173597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 237.0 1 237.0 2 550.5 3 864.0 4 864.0 5 864.0 6 1382.5 7 1901.0 8 1901.0 9 1901.0 10 2154.5 11 2408.0 12 2408.0 13 2408.0 14 3504.0 15 4600.0 16 4600.0 17 4600.0 18 9513.0 19 14426.0 20 14426.0 21 14426.0 22 24770.5 23 35115.0 24 35115.0 25 35115.0 26 55169.0 27 75223.0 28 75223.0 29 75223.0 30 100367.0 31 125511.0 32 125511.0 33 125511.0 34 147177.0 35 168843.0 36 168843.0 37 168843.0 38 187777.5 39 206712.0 40 206712.0 41 206712.0 42 225835.0 43 244958.0 44 244958.0 45 244958.0 46 265734.5 47 286511.0 48 286511.0 49 286511.0 50 284706.5 51 282902.0 52 282902.0 53 282902.0 54 258379.5 55 233857.0 56 233857.0 57 233857.0 58 204976.5 59 176096.0 60 176096.0 61 176096.0 62 150401.0 63 124706.0 64 124706.0 65 124706.0 66 97333.0 67 69960.0 68 69960.0 69 69960.0 70 53202.0 71 36444.0 72 36444.0 73 36444.0 74 26094.0 75 15744.0 76 15744.0 77 15744.0 78 10863.5 79 5983.0 80 5983.0 81 5983.0 82 4002.5 83 2022.0 84 2022.0 85 2022.0 86 1277.0 87 532.0 88 532.0 89 532.0 90 340.5 91 149.0 92 149.0 93 149.0 94 85.0 95 21.0 96 21.0 97 21.0 98 15.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3394092360338133 2 0.01072913195650684 3 0.009642039291310111 4 0.01148537033229587 5 0.012288873606571712 6 0.08091750620942603 7 0.07997220823968976 8 0.1043136309603991 9 0.08479322788534481 10 0.09183569775988014 11 0.08696741321573827 12 0.097980134563166 13 0.0840842544080426 14 0.08847988996731632 15 0.08673108872330419 16 0.08795997608396136 17 0.09930355172079679 18 0.10218671052849246 19 0.09580594923277254 20 0.09405714798876041 21 0.08299716174284587 22 0.1019503860360584 23 0.09594774392823298 24 0.09150484347047244 25 0.08999236671889438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2115735.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.06960429668799 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38461122907776 42.8285492597074 2 12.93417585977285 14.504282447224758 3 4.517393933273057 7.598654708726378 4 2.0789975693589335 4.662742841909262 5 1.1688467196155377 3.2768386526162505 6 0.6961227155759875 2.34187951225689 7 0.4615994347263457 1.8117188354076743 8 0.32152627246541715 1.4422280694500018 9 0.23542693728549222 1.1880265692940857 >10 1.1003136421931075 10.674446330580507 >50 0.06194503305543171 2.366961100499898 >100 0.03284777928508981 3.4931095165213364 >500 0.004326528631015146 1.6978218420606725 >1k 0.0018663456839672892 2.112740313744833 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4197 0.19837077894915953 No Hit GTCCTACAGTGGACATTTCTAAATT 4143 0.19581847443087153 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3683 0.17407662112693698 No Hit CTGTAGGACGTGGAATATGGCAAGA 3323 0.15706125767168386 No Hit CTTTAGGACGTGAAATATGGCGAGG 2650 0.12525198099005783 No Hit GTACTGGTTCACTATCGGTCAGTCA 2456 0.11608259068361586 No Hit TATCAACGCAGAGTACTTTTTTTTT 2397 0.11329396167289382 No Hit GGTATCAACGCAGAGTACTTTTTTT 2302 0.10880379631664645 No Hit GTCCTACAGTGTGCATTTCTCATTT 2238 0.10577884281349034 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.726489848681427E-5 0.0 10 0.0 0.0 0.0 4.726489848681427E-5 0.0 11 0.0 0.0 0.0 4.726489848681427E-5 0.0 12 0.0 0.0 0.0 4.726489848681427E-5 4.726489848681427E-5 13 0.0 0.0 0.0 4.726489848681427E-5 4.726489848681427E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTACG 40 0.0052029854 14.281567 1 TCGTGCA 50 0.001501619 13.297931 9 CCGAATG 115 5.335096E-9 12.390256 18 TAGGACC 655 0.0 11.7462635 4 GACGCTC 90 7.4769505E-6 11.609303 8 GTCCTAG 280 0.0 11.561268 1 GGTATCA 1510 0.0 11.349589 1 TCTATAC 135 4.7421054E-9 11.257507 3 TGGCGAG 1870 0.0 10.972333 18 GTACTAG 105 3.3768029E-6 10.881194 1 GTATCAA 3195 0.0 10.757737 1 CTAGCAC 125 2.2458335E-7 10.638344 3 CTGTAGG 1990 0.0 10.525777 1 GGCGAGG 895 0.0 10.401108 19 TGTAGGA 2060 0.0 10.3745985 2 TTTATAC 165 9.313226E-10 10.362023 3 ATGGCGA 2100 0.0 10.358646 17 CGTTTCC 120 1.5258574E-6 10.290795 14 GGCGAGA 1180 0.0 10.223469 19 TAGGACG 2210 0.0 10.186197 4 >>END_MODULE