FastQCFastQC Report
Thu 26 May 2016
SRR1512902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115735
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53350.25215823342715415No Hit
TATCAACGCAGAGTACTTTTTTTTT39110.18485301798193063No Hit
GTCCTACAGTGGACATTTCTAAATT37640.17790507790436894No Hit
CTGTAGGACGTGGAATATGGCAAGA33940.16041706546424767No Hit
GTCCTAAAGTGTGTATTTCTCATTT33180.15682493317924975No Hit
GGTATCAACGCAGAGTACTTTTTTT30400.1436852913999154No Hit
CTTTAGGACGTGAAATATGGCGAGG29630.14004589421643068No Hit
GTACTGGTTCACTATCGGTCAGTCA23230.10979635918486957No Hit
GTCCTACAGTGTGCATTTCTCATTT21470.10147773705119026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7650.012.678144
GGTATCA16150.012.4845441
TAAGACG701.082075E-412.2253494
GGACCGT1459.640644E-1111.8037856
CCTATAC1701.0913936E-1111.1876543
TGTAGGA22050.010.824882
ATCGCGC2250.010.5566218
GTAGGAC21650.010.5417153
GTATCAA34500.010.5306121
GGCGAGG9950.010.49463619
CCCTATA1002.3731574E-510.4604572
CTGTAGG23300.010.408631
AGGACCT14500.010.36115
TGGCGAG22750.010.34852218
AGGACGT20150.010.2872245
TTAGGAC11750.010.2783583
GATATAC3250.010.2422181
CCCTAGA1303.784171E-710.2422171
GACGTGG9600.010.09954557
GGACGTG20550.010.08698756
Produced by FastQC (version 0.11.5)