##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512902_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115735 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.340285054602774 31.0 30.0 34.0 26.0 34.0 2 30.331666300363704 31.0 30.0 34.0 26.0 34.0 3 30.521694352080956 31.0 31.0 34.0 27.0 34.0 4 34.145250232188815 37.0 35.0 37.0 30.0 37.0 5 33.75950721616837 37.0 35.0 37.0 28.0 37.0 6 33.654526204841346 37.0 35.0 37.0 28.0 37.0 7 33.4942400631459 37.0 35.0 37.0 28.0 37.0 8 33.446113525559674 37.0 35.0 37.0 28.0 37.0 9 34.79695661318643 39.0 35.0 39.0 27.0 39.0 10 34.71157777320884 39.0 35.0 39.0 27.0 39.0 11 34.71860653626281 39.0 35.0 39.0 27.0 39.0 12 34.596428664270334 39.0 35.0 39.0 27.0 39.0 13 34.58790585777519 39.0 35.0 39.0 27.0 39.0 14 35.668172526332455 40.0 36.0 41.0 27.0 41.0 15 35.665494969833176 40.0 36.0 41.0 27.0 41.0 16 35.660831814948466 40.0 36.0 41.0 27.0 41.0 17 35.63328630475934 40.0 36.0 41.0 27.0 41.0 18 35.554866275785955 40.0 36.0 41.0 27.0 41.0 19 35.550281580632735 40.0 36.0 41.0 27.0 41.0 20 35.5304482839297 40.0 36.0 41.0 27.0 41.0 21 35.49247046534656 40.0 36.0 41.0 26.0 41.0 22 35.45496387780133 40.0 36.0 41.0 26.0 41.0 23 35.30127922447755 40.0 35.0 41.0 25.0 41.0 24 35.26110453341274 40.0 35.0 41.0 25.0 41.0 25 35.194467643632116 40.0 35.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6762436297355237 1101 2 0.5395809418977677 1101 3 0.5944279406005393 1101 4 0.5658777895947935 1101 5 0.7047729502457685 1101 6 0.7339886951756185 1101 7 0.8622103653565816 1101 8 0.6553446896474142 1101 9 0.9465241723968489 1101 10 0.6820785418230741 1101 11 0.9837439864623434 1101 12 1.0668363725975993 1101 13 1.2619875612023321 1101 14 1.309026918653288 1101 15 0.9993703589971261 1101 16 1.2080027701372131 1101 17 1.2860776308052948 1101 18 0.9738194951706305 1101 19 0.9764507937863129 1101 20 0.9770537535717665 1101 21 1.0373967399135822 1101 22 0.9597646154801609 1101 23 1.0904042392398523 1101 24 1.156085237281708 1101 25 1.2881668550050662 1102 1 0.7730394978274262 1102 2 0.914288864207748 1102 3 0.6171597789190173 1102 4 0.6855519313357306 1102 5 0.8977139214767789 1102 6 0.7748314648650947 1102 7 0.8594666853362867 1102 8 0.9370339295581829 1102 9 1.0293066551365087 1102 10 0.986188775379496 1102 11 1.0616385953161327 1102 12 1.0867542207912138 1102 13 1.2293969774698752 1102 14 1.1596824414327074 1102 15 1.3074803388027973 1102 16 1.2938915850154658 1102 17 1.360482109506087 1102 18 0.6400045640533918 1102 19 1.0207625477326658 1102 20 1.2384646077602426 1102 21 1.181320995313719 1102 22 0.9336319884197621 1102 23 1.167081901772498 1102 24 1.1526913209560163 1102 25 1.2101199956992872 1103 1 0.7387473763774892 1103 2 0.9365831403924219 1103 3 0.6718774776898542 1103 4 0.4658724223123514 1103 5 0.6589569211704358 1103 6 0.720101374648479 1103 7 0.8075895534052506 1103 8 0.8251655060598679 1103 9 0.9506263096905414 1103 10 0.8020617011031064 1103 11 1.0084773998169752 1103 12 1.1311507057510966 1103 13 1.068937564617876 1103 14 1.170807894494942 1103 15 1.2483523179576324 1103 16 1.1664753391416482 1103 17 1.1804662415713665 1103 18 1.1665279374179747 1103 19 1.1436963602019006 1103 20 1.0156061765861182 1103 21 1.200384029909948 1103 22 1.0724280562009696 1103 23 1.173605481661177 1103 24 1.2691149776028254 1103 25 1.2451714374095815 1104 1 0.5861146099551959 1104 2 0.7266292088963446 1104 3 0.618714292142009 1104 4 0.48462589843807535 1104 5 0.5572182743705341 1104 6 0.5118717538428115 1104 7 0.48718579981571963 1104 8 0.5055783352360663 1104 9 0.5582958236067199 1104 10 0.6291300530327604 1104 11 0.5596499480122006 1104 12 0.7184010805718088 1104 13 0.5869303975786764 1104 14 0.7649064274048598 1104 15 0.7735272540375391 1104 16 0.7052132164969933 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-3.8464040061913103 2216 22 -3.3239334802455787 2216 23 -4.511769461781775 2216 24 -3.8290087573053775 2216 25 -3.9407174012063813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53002.0 3 2.0 4 844.0 5 7163.0 6 8800.0 7 6852.0 8 5540.0 9 4704.0 10 4048.0 11 3788.0 12 3497.0 13 3343.0 14 3242.0 15 3372.0 16 3432.0 17 3632.0 18 4101.0 19 4854.0 20 6258.0 21 8292.0 22 10648.0 23 12876.0 24 15622.0 25 17681.0 26 20202.0 27 22532.0 28 25381.0 29 30755.0 30 38464.0 31 49028.0 32 62292.0 33 80144.0 34 104263.0 35 135892.0 36 184813.0 37 283551.0 38 882825.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.86080155468627 18.803027815846505 13.49578757793182 26.84038305153541 2 14.117327192644414 19.890488946050674 39.52710705934922 26.46507680195569 3 17.870290309663954 24.590919555825128 28.97764044981003 28.561149684700894 4 12.26141346554253 16.300007988137853 35.899542312788064 35.539036233531554 5 13.692144561363037 38.216652949388205 33.94262947227794 14.14857301697082 6 33.27542883303593 35.39531999842981 17.44191380915919 13.887337359375065 7 29.654929377595717 30.971211791529097 20.704331948951307 18.669526881923883 8 26.920128354587824 35.66583351265456 19.546502526655804 17.867535606101807 9 27.604472746476443 14.355254380245858 19.075457148787233 38.964815724490464 10 16.639837473730406 28.301929621131443 32.34252903133057 22.715703873807584 11 35.614453776587276 21.66535027204701 23.468645465386857 19.25155048597886 12 24.47546498113418 24.099991917687078 30.0495667927539 21.37497630842484 13 29.722132413694002 21.038356749725377 25.291830449061546 23.94768038751907 14 22.058358521823592 21.350465445487696 26.34801946386919 30.243156568819522 15 24.225168618359973 29.914135579183032 22.27624858893803 23.584447213518967 16 23.31106488197626 26.952105576377573 26.387665802115666 23.349163739530496 17 22.628763306936104 27.820418164647247 26.507142566786378 23.04367596163027 18 22.60001569281411 26.31401321032436 28.769796815871295 22.316174280990236 19 24.600876212425504 25.81659850158128 26.569163731857632 23.013361554135585 20 25.094046665295966 25.815555571310746 26.918733944477136 22.171663818916155 21 24.441587537162114 25.34316760645262 26.093925028240843 24.121319828144422 22 24.737359782240336 26.56940143978878 26.09887759621161 22.59436118175927 23 23.581544947736838 26.34800672106857 26.84050696330117 23.229941367893428 24 24.41279271742444 25.67032260656462 27.032166126665203 22.884718549345738 25 23.687040201159405 26.11692863236653 26.976015427262865 23.2200157392112 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 72.0 1 72.0 2 216.5 3 361.0 4 361.0 5 361.0 6 877.5 7 1394.0 8 1394.0 9 1394.0 10 1704.5 11 2015.0 12 2015.0 13 2015.0 14 3187.5 15 4360.0 16 4360.0 17 4360.0 18 9542.0 19 14724.0 20 14724.0 21 14724.0 22 25622.0 23 36520.0 24 36520.0 25 36520.0 26 57338.0 27 78156.0 28 78156.0 29 78156.0 30 103338.0 31 128520.0 32 128520.0 33 128520.0 34 149509.5 35 170499.0 36 170499.0 37 170499.0 38 189531.0 39 208563.0 40 208563.0 41 208563.0 42 227599.0 43 246635.0 44 246635.0 45 246635.0 46 267471.5 47 288308.0 48 288308.0 49 288308.0 50 285441.0 51 282574.0 52 282574.0 53 282574.0 54 257405.5 55 232237.0 56 232237.0 57 232237.0 58 203465.0 59 174693.0 60 174693.0 61 174693.0 62 148038.5 63 121384.0 64 121384.0 65 121384.0 66 94644.0 67 67904.0 68 67904.0 69 67904.0 70 50982.5 71 34061.0 72 34061.0 73 34061.0 74 24481.5 75 14902.0 76 14902.0 77 14902.0 78 10230.5 79 5559.0 80 5559.0 81 5559.0 82 3638.0 83 1717.0 84 1717.0 85 1717.0 86 1084.5 87 452.0 88 452.0 89 452.0 90 280.5 91 109.0 92 109.0 93 109.0 94 62.0 95 15.0 96 15.0 97 15.0 98 8.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08829083037336906 2 0.04159311066839656 3 0.02089108513117191 4 0.004631960051707799 5 0.01881142959775208 6 0.06305137458141025 7 0.07916870496541391 8 0.06049907006312227 9 0.029020647670903964 10 0.053078481000692436 11 0.0051046090365759415 12 1.890595939472571E-4 13 0.005813582513878156 14 9.452979697362855E-4 15 0.014510323835451982 16 0.008791271118547455 17 0.005151873935062756 18 0.00552999312295727 19 0.0017960661424989426 20 0.0014179469546044283 21 2.3632449243407138E-4 22 3.781191878945142E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2115735.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.10317522306464 #Duplication Level Percentage of deduplicated Percentage of total 1 73.73101759594417 39.15351146772284 2 14.28780307246092 15.174554202190668 3 5.038324575755935 8.026530983311206 4 2.3521869333986096 4.996343795266778 5 1.283388712833034 3.4076007848437992 6 0.7938331004758276 2.5293034939461925 7 0.5258190619121051 1.9545863246242197 8 0.34441405648107837 1.4631583992480948 9 0.26742995742566217 1.2781241900164488 >10 1.2615470657879437 11.62786274469286 >50 0.07010524298101148 2.536016658232038 >100 0.03762508178487885 3.7847698090812716 >500 0.004366734180586108 1.6894183856812093 >1k 0.0020496915541526506 2.125745384083978 >5k 8.911702409359352E-5 0.25247337705843903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5335 0.25215823342715415 No Hit TATCAACGCAGAGTACTTTTTTTTT 3911 0.18485301798193063 No Hit GTCCTACAGTGGACATTTCTAAATT 3764 0.17790507790436894 No Hit CTGTAGGACGTGGAATATGGCAAGA 3394 0.16041706546424767 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3318 0.15682493317924975 No Hit GGTATCAACGCAGAGTACTTTTTTT 3040 0.1436852913999154 No Hit CTTTAGGACGTGAAATATGGCGAGG 2963 0.14004589421643068 No Hit GTACTGGTTCACTATCGGTCAGTCA 2323 0.10979635918486957 No Hit GTCCTACAGTGTGCATTTCTCATTT 2147 0.10147773705119026 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.452979697362855E-5 0.0 10 0.0 0.0 0.0 9.452979697362855E-5 0.0 11 0.0 0.0 0.0 9.452979697362855E-5 0.0 12 0.0 0.0 0.0 9.452979697362855E-5 4.726489848681427E-5 13 0.0 0.0 0.0 9.452979697362855E-5 4.726489848681427E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 765 0.0 12.67814 4 GGTATCA 1615 0.0 12.484544 1 TAAGACG 70 1.082075E-4 12.225349 4 GGACCGT 145 9.640644E-11 11.803785 6 CCTATAC 170 1.0913936E-11 11.187654 3 TGTAGGA 2205 0.0 10.82488 2 ATCGCGC 225 0.0 10.556621 8 GTAGGAC 2165 0.0 10.541715 3 GTATCAA 3450 0.0 10.530612 1 GGCGAGG 995 0.0 10.494636 19 CCCTATA 100 2.3731574E-5 10.460457 2 CTGTAGG 2330 0.0 10.40863 1 AGGACCT 1450 0.0 10.3611 5 TGGCGAG 2275 0.0 10.348522 18 AGGACGT 2015 0.0 10.287224 5 TTAGGAC 1175 0.0 10.278358 3 GATATAC 325 0.0 10.242218 1 CCCTAGA 130 3.784171E-7 10.242217 1 GACGTGG 960 0.0 10.0995455 7 GGACGTG 2055 0.0 10.0869875 6 >>END_MODULE