FastQCFastQC Report
Thu 26 May 2016
SRR1512901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027245
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66510.32808072038653446No Hit
GTCCTACAGTGGACATTTCTAAATT55190.27224139164235206No Hit
CTGTAGGACGTGGAATATGGCAAGA48550.23948758043551718No Hit
GTCCTAAAGTGTGTATTTCTCATTT48540.23943825240659122No Hit
CTTTAGGACGTGAAATATGGCGAGG42820.21122261986094426No Hit
TATCAACGCAGAGTACTTTTTTTTT38620.1905048477120427No Hit
GGTATCAACGCAGAGTACTTTTTTT36870.18187244265000038No Hit
GTACTGGTTCACTATCGGTCAGTCA36190.17851813668303534No Hit
GTCCTACAGTGTGCATTTCTCATTT28280.13949966580260403No Hit
CTGAAGGACCTGGAATATGGCGAGA23650.11666078840988632No Hit
GGATACCACGTGTCCCGCCCTACTC21580.10644988642221341No Hit
CTGTAGGACCTGGAATATGGCGAGA20880.10299692439739647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG400.00528548114.24770459
GGTATCA18200.012.9769311
TAGGACC8150.012.1204474
CACCGTA550.003072136312.0892597
GCGCCAC1157.088056E-811.56392513
CCAACGA1501.7644197E-1011.39844419
TGCGTTT600.00588159211.08209412
GTCCTAA13150.011.0804481
GTCCTAG2800.010.8838771
GGCGTGC1752.0008883E-1110.8556618
GGCGAGG12450.010.75756819
CGTGCGC1252.2457971E-710.63828710
GTATCAA39350.010.5761691
TCCAACG2250.010.55463618
GTCCTAC26300.010.537291
TAGGACA7600.010.4980494
CTAGGAC3550.010.434683
GTAGGAC26250.010.3485533
TGTAGGA27600.010.2897542
GTATAAT3150.010.2792181
Produced by FastQC (version 0.11.5)