##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512901_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2027245
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.688495470453745	31.0	30.0	34.0	18.0	34.0
2	28.85339413835032	31.0	30.0	34.0	16.0	34.0
3	28.958964999297077	31.0	30.0	34.0	20.0	34.0
4	32.353134426278025	35.0	33.0	37.0	22.0	37.0
5	32.52447336163118	35.0	33.0	37.0	25.0	37.0
6	32.43953592190387	36.0	33.0	37.0	23.0	37.0
7	32.37623474222405	36.0	33.0	37.0	22.0	37.0
8	32.294748291400396	37.0	33.0	37.0	21.0	37.0
9	33.55695636195921	38.0	34.0	39.0	18.0	39.0
10	33.42917802238999	38.0	33.0	39.0	17.0	39.0
11	33.47781743203214	38.0	34.0	39.0	18.0	39.0
12	33.37126001050687	38.0	34.0	39.0	17.0	39.0
13	33.33925055925653	38.0	34.0	39.0	17.0	39.0
14	34.17388376836544	39.0	33.0	41.0	17.0	41.0
15	34.354753372187375	39.0	34.0	41.0	17.0	41.0
16	34.295110852413	39.0	34.0	41.0	17.0	41.0
17	34.06542080508276	39.0	33.0	41.0	16.0	41.0
18	34.06963144563188	39.0	33.0	41.0	16.0	41.0
19	34.17792866673737	39.0	34.0	41.0	16.0	41.0
20	34.153602549272534	39.0	34.0	41.0	11.0	41.0
21	34.04364346687253	39.0	34.0	41.0	10.0	41.0
22	34.0533768735402	39.0	34.0	41.0	10.0	41.0
23	33.7168852309415	39.0	33.0	41.0	2.0	41.0
24	33.785858147387216	39.0	34.0	41.0	2.0	41.0
25	33.49757528073815	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.42041705779192995
1101	2	0.1698434064968417
1101	3	0.5135359473262184
1101	4	0.678361119183073
1101	5	0.4885796519632848
1101	6	0.5537203318543575
1101	7	0.38100705303736504
1101	8	0.5855720787152947
1101	9	0.6709097296307149
1101	10	0.6465354741862157
1101	11	0.7199938304645812
1101	12	0.49118069844131895
1101	13	0.9646803897753102
1101	14	0.9538230307574764
1101	15	0.8456096895292404
1101	16	0.759965808569298
1101	17	0.16815185329319604
1101	18	0.681281096801527
1101	19	0.16934344699964043
1101	20	-0.4799728800140528
1101	21	0.6278202323160897
1101	22	0.6380854370190718
1101	23	0.2150378048125745
1101	24	0.7202236094098566
1101	25	0.4217229899515047
1102	1	0.9336481777094434
1102	2	1.0361566324016103
1102	3	0.9040757079339556
1102	4	1.087589696134458
1102	5	0.9950060571742938
1102	6	0.818895704988293
1102	7	0.8756069977398866
1102	8	0.9556984341923567
1102	9	1.1571863375833118
1102	10	1.2364536818469318
1102	11	0.9888921237487693
1102	12	1.1164644833478476
1102	13	1.0033442853042516
1102	14	1.1891936120133622
1102	15	1.215493551100586
1102	16	1.1358509984202811
1102	17	1.3962553239753461
1102	18	1.4697816494068192
1102	19	1.266993853132064
1102	20	1.4418585707804255
1102	21	1.5153195289570789
1102	22	1.3473401002761136
1102	23	1.2164058842745717
1102	24	0.7355488653137741
1102	25	0.9590122079039034
1103	1	0.3108932329298888
1103	2	0.3865082219375857
1103	3	0.5667894309991865
1103	4	0.5918857324699474
1103	5	0.5821222884671258
1103	6	0.2417992923721215
1103	7	0.1446654896973314
1103	8	0.22696076623213202
1103	9	0.3189712467167709
1103	10	0.5034113273043772
1103	11	0.37399554883171504
1103	12	0.5118548224486545
1103	13	0.6901775270596318
1103	14	0.6901018879321583
1103	15	0.5480015530847808
1103	16	0.6582754228323466
1103	17	0.2523132941863224
1103	18	0.4129036964983541
1103	19	0.41150437107343407
1103	20	0.6254053763555589
1103	21	0.3644631696952274
1103	22	0.45534647535627926
1103	23	-0.27336259363912774
1103	24	-0.015195232966796368
1103	25	0.3094935005563215
1104	1	0.8296345787086636
1104	2	0.7537610787262139
1104	3	0.755026815614098
1104	4	0.7908934624788984
1104	5	0.7051236828276402
1104	6	0.7047549512334044
1104	7	0.8521922198818999
1104	8	0.5483113025751933
1104	9	0.974533859670693
1104	10	0.9136172872690622
1104	11	0.6207685020920266
1104	12	0.9723956256038662
1104	13	1.0059156363289077
1104	14	1.1857360244944957
1104	15	0.9697003880144948
1104	16	0.9117085748134954
1104	17	1.1719442081738762
1104	18	0.8945218860197102
1104	19	0.47820953443716263
1104	20	0.6255781272364302
1104	21	0.7696213054365089
1104	22	0.4868085812942766
1104	23	1.0647764030241547
1104	24	0.9710265006950323
1104	25	0.4280004071820045
1105	1	0.846772078603184
1105	2	0.8817376828589971
1105	3	1.0312071834577026
1105	4	1.114982420235961
1105	5	0.9914931867275172
1105	6	1.1157675633105555
1105	7	1.1068309042833704
1105	8	1.1283793652901934
1105	9	0.8883514133023027
1105	10	0.8214568755911884
1105	11	0.9222659419566526
1105	12	1.0532815679384342
1105	13	1.0921222335452399
1105	14	1.1080020102947685
1105	15	1.0316531324673903
1105	16	1.109353197898777
1105	17	1.5082605753587117
1105	18	1.3220140086241585
1105	19	0.904683053808327
1105	20	1.0903725438707639
1105	21	0.9430245876434356
1105	22	1.0250727871091598
1105	23	1.226697654383365
1105	24	1.3479249246533698
1105	25	1.6297107139782767
1106	1	0.836433693687507
1106	2	0.8290932750462332
1106	3	0.7649438421227153
1106	4	0.8817264656291002
1106	5	0.7631154734270496
1106	6	0.919406277346134
1106	7	0.8339584895365704
1106	8	0.9242960731352738
1106	9	0.9071113168223519
1106	10	0.8474684440231641
1106	11	0.5835227418490021
1106	12	0.9611648864118365
1106	13	1.0189081544844711
1106	14	1.2207114685970737
1106	15	1.018112004082667
1106	16	1.0487952955950917
1106	17	1.2079709232734217
1106	18	1.1251579867038544
1106	19	1.1430989784950185
1106	20	1.3190576697715883
1106	21	1.3189298061904893
1106	22	1.189268302712975
1106	23	1.5110436990293934
1106	24	1.1034103306368266
1106	25	1.3433010382955928
1107	1	0.912990098780341
1107	2	0.920767388510054
1107	3	0.9032862815592679
1107	4	0.9023166516075349
1107	5	0.8444138304691649
1107	6	0.9857798913627462
1107	7	0.714071079428301
1107	8	0.9079440722168783
1107	9	0.8136834716095507
1107	10	1.084103218513775
1107	11	0.9234832104002066
1107	12	1.0467110278052871
1107	13	0.814517007656157
1107	14	1.088898408330273
1107	15	0.9769354522519293
1107	16	0.9555784388869455
1107	17	1.2523407087400358
1107	18	1.1501919588896143
1107	19	0.9760368308220677
1107	20	1.1289125568932334
1107	21	0.9224191289869381
1107	22	0.9731634737503043
1107	23	1.2594683132970559
1107	24	1.1257831441900876
1107	25	1.412369702320106
1108	1	1.0144321439934103
1108	2	0.8222037359702341
1108	3	0.847329491075449
1108	4	0.9262083569403003
1108	5	0.7633793613993234
1108	6	0.9617617145969675
1108	7	0.8979998668577522
1108	8	0.9350225961252505
1108	9	1.0636329624312637
1108	10	1.1154658921609624
1108	11	0.7150678161927644
1108	12	1.011113335862234
1108	13	1.0035374378365063
1108	14	1.0483631931817214
1108	15	0.9780949916680015
1108	16	0.8191720863595009
1108	17	1.1924461171685081
1108	18	1.1231776047555897
1108	19	1.0263758237675944
1108	20	1.1286428943916818
1108	21	1.0958755400097502
1108	22	1.1529368246127945
1108	23	1.3632767006695303
1108	24	1.3155253208717994
1108	25	1.4577073759089174
1109	1	0.8155498693243182
1109	2	0.7599724872833491
1109	3	0.7849754513434419
1109	4	0.9242455677627248
1109	5	0.7118762215902805
1109	6	0.8709654403480727
1109	7	0.7944669397153277
1109	8	0.8289778217479622
1109	9	0.9233462943639381
1109	10	0.9035075384013638
1109	11	0.8857380311938172
1109	12	0.7337353682174381
1109	13	0.8676791844574154
1109	14	0.9686255831716863
1109	15	0.8575571388771905
1109	16	0.42766147637281193
1109	17	1.058958711421134
1109	18	0.9488936913162718
1109	19	0.8961622233754056
1109	20	0.7618440989578303
1109	21	0.8447468428554217
1109	22	0.8532734494931518
1109	23	1.1077849528814028
1109	24	0.8664766013069354
1109	25	0.45153794294208893
1110	1	0.6480485795371891
1110	2	0.67370199088354
1110	3	0.6726538718962942
1110	4	0.6676353152635244
1110	5	0.5720440434575877
1110	6	0.5675861980699679
1110	7	0.5801112351177053
1110	8	0.5599431806826374
1110	9	0.7347691351855161
1110	10	0.7712537008173399
1110	11	0.719852339980072
1110	12	0.7248005080752264
1110	13	0.8189549521611426
1110	14	0.7671146629739951
1110	15	0.7464644087903878
1110	16	0.7236513599555039
1110	17	0.9653366153114362
1110	18	0.28138511142797995
1110	19	0.6528186465153141
1110	20	0.7447617095991319
1110	21	0.9062317429997293
1110	22	1.0061337861089044
1110	23	1.0614200964029337
1110	24	0.9822020972207426
1110	25	1.055169347950205
1111	1	0.4408247654746589
1111	2	0.32694885176787736
1111	3	0.5292927833143288
1111	4	0.47306860498569847
1111	5	0.4273649999036593
1111	6	0.310382216232739
1111	7	0.2852896659446742
1111	8	0.5281960800355705
1111	9	0.5445884547329811
1111	10	0.4993043114722795
1111	11	0.29063340405295435
1111	12	-0.0326062360653836
1111	13	0.39151795647687493
1111	14	0.4426439169863201
1111	15	0.3474415158903241
1111	16	0.44374540022379705
1111	17	-0.14371546352018072
1111	18	0.2026778173131163
1111	19	0.3254167272295092
1111	20	0.47334808634037273
1111	21	0.5575301617497672
1111	22	0.20820788167062432
1111	23	0.6002187371554868
1111	24	0.4515598291245766
1111	25	0.4386424442045751
1112	1	0.5173543897279202
1112	2	0.4326249833251019
1112	3	0.410816154100516
1112	4	0.6026566041528767
1112	5	0.6181104874338459
1112	6	0.39670426760289246
1112	7	0.2230862324345395
1112	8	0.4886658326195885
1112	9	0.488684533903772
1112	10	0.4598612413184213
1112	11	0.4594651850607647
1112	12	0.4503390522856918
1112	13	0.5393811489628106
1112	14	0.3944751126870756
1112	15	0.15915919551188296
1112	16	0.3718614686493922
1112	17	0.7383688265183892
1112	18	0.3859153223908791
1112	19	0.6315304922826002
1112	20	0.42810381528788355
1112	21	0.5383730038959484
1112	22	0.3101142791348792
1112	23	0.482179814236936
1112	24	0.51238972026038
1112	25	0.6233042692268853
1113	1	-0.2763008831165479
1113	2	-0.10887762219091712
1113	3	-0.10403312437696854
1113	4	-0.10176691579725627
1113	5	-0.1645582501055003
1113	6	-0.617400829680907
1113	7	-0.5822654115124948
1113	8	-0.4787218212686746
1113	9	-0.4761970199636423
1113	10	-0.2831594492765248
1113	11	-0.4109403523824966
1113	12	-0.540534354853726
1113	13	-0.4010462971302289
1113	14	-0.10228277473316894
1113	15	-0.4772131917549274
1113	16	-0.563824456254487
1113	17	-0.48406746137920464
1113	18	-0.5613362754590625
1113	19	-0.8425185904446906
1113	20	-0.6749109307687533
1113	21	-0.41019857107438895
1113	22	-1.0409731796529655
1113	23	-4.586738029161797
1113	24	-2.159807514396835
1113	25	-1.4210370297011465
1114	1	-0.6572463397641073
1114	2	-0.8631700748763613
1114	3	-0.6287873892125653
1114	4	-0.5715959402874731
1114	5	-0.6603321922646295
1114	6	-1.402649662116552
1114	7	-1.178256420022219
1114	8	-0.9705599819666659
1114	9	-1.216972046514158
1114	10	-0.8683876134400208
1114	11	-1.0617651381941613
1114	12	-1.9793995756454166
1114	13	-1.269499574907357
1114	14	-3.2696072857102685
1114	15	-1.9265391516887647
1114	16	-1.9212185751609994
1114	17	-1.6694708703176957
1114	18	-1.6437162665919018
1114	19	-1.6926085835463596
1114	20	-1.306137581832587
1114	21	-1.177530743566166
1114	22	-1.2964559882137863
1114	23	-5.889397257758134
1114	24	-2.808856392781774
1114	25	-2.5998207513779157
1115	1	-1.5658033487703626
1115	2	-1.544608433435517
1115	3	-1.2468387497091804
1115	4	-1.2566706163810508
1115	5	-1.1065483588769673
1115	6	-1.2742806108999716
1115	7	-1.8706131406626447
1115	8	-2.1865791131192402
1115	9	-3.3278419448313734
1115	10	-4.015207264352345
1115	11	-2.823611150997195
1115	12	-3.013600056756978
1115	13	-2.32940952323149
1115	14	-2.9971848710658726
1115	15	-2.649030963709585
1115	16	-2.739747184381713
1115	17	-2.7002412500337165
1115	18	-5.272158761260442
1115	19	-3.2083688153879706
1115	20	-3.5590567238110147
1115	21	-3.374575612810574
1115	22	-2.7536539070749697
1115	23	-3.2693710954474113
1115	24	-3.147359689516197
1115	25	-3.25910533588954
1116	1	-1.4907853447679038
1116	2	-1.3692610909976253
1116	3	-1.8122802370791646
1116	4	-1.6129660201066294
1116	5	-1.58390978097394
1116	6	-3.79711603592294
1116	7	-2.506310806467031
1116	8	-2.7315970818444377
1116	9	-3.770615322190732
1116	10	-3.1835097376411596
1116	11	-2.6076573621940966
1116	12	-2.4828801637579545
1116	13	-3.8110702356003543
1116	14	-6.927220284396945
1116	15	-4.950671305979654
1116	16	-3.725711168313424
1116	17	-4.517883509370975
1116	18	-3.2184709015268673
1116	19	-3.0129507269216695
1116	20	-3.7405729505954284
1116	21	-4.464325199688641
1116	22	-4.0851603822163405
1116	23	-3.0873714693863867
1116	24	-4.194540305568058
1116	25	-4.091170250082641
1201	1	0.7789799163324354
1201	2	0.8512058215259302
1201	3	1.1560422719760979
1201	4	1.138118266986531
1201	5	0.5802286339766596
1201	6	0.9696806729791589
1201	7	1.207146705614285
1201	8	1.168318253277043
1201	9	1.71273264291505
1201	10	1.4324961818943294
1201	11	1.659945091201152
1201	12	1.3503643010937267
1201	13	1.4747888973472172
1201	14	1.5767403181410629
1201	15	1.5857344301062852
1201	16	1.5544582261144342
1201	17	1.7941014481968836
1201	18	1.5748272616602392
1201	19	1.7414718677685315
1201	20	1.6680448669692751
1201	21	1.903650350430972
1201	22	1.5941650580437212
1201	23	2.014377606764043
1201	24	1.8171077709332053
1201	25	1.9461009432178926
1202	1	1.1396531803137933
1202	2	1.118187286755692
1202	3	1.2113217035246038
1202	4	1.4224944699690525
1202	5	1.2323794458306736
1202	6	1.4582700310854193
1202	7	1.4829649271765035
1202	8	1.381863919810236
1202	9	1.726663650745465
1202	10	1.546964449378109
1202	11	1.5256997103853749
1202	12	1.7285357955959313
1202	13	1.6571123110653048
1202	14	2.1266235099483026
1202	15	1.764047203521173
1202	16	1.6339294952108858
1202	17	1.8597026444572151
1202	18	1.930231099468699
1202	19	1.9822318335417677
1202	20	1.7900063117756062
1202	21	1.9730805641720224
1202	22	1.8143716609897922
1202	23	2.0915746142217557
1202	24	1.91980301063127
1202	25	2.2469082083027416
1203	1	0.9996994176840239
1203	2	1.120155030147906
1203	3	1.0181953832632864
1203	4	1.137828777278223
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2211	20	-0.18715798070756762
2211	21	-0.02106243316770673
2211	22	-0.11811689045624263
2211	23	0.168253133807454
2211	24	-0.22551818551043823
2211	25	0.37174511667584653
2212	1	0.10702468324157621
2212	2	0.26357874197148234
2212	3	-0.10740525375147314
2212	4	0.2073567558072682
2212	5	0.028508268211936638
2212	6	0.03592129979068659
2212	7	-0.04335257445004004
2212	8	0.08327359233246767
2212	9	0.14130255770707834
2212	10	-0.2719468496893427
2212	11	-0.2821836121043191
2212	12	0.0952349931669545
2212	13	0.16772822952117394
2212	14	0.20336391333151482
2212	15	-0.194237333455618
2212	16	-0.11276319617926589
2212	17	0.053586834437695074
2212	18	0.1781274343201389
2212	19	-0.07336348200800558
2212	20	0.16033812700006678
2212	21	0.07209379340645938
2212	22	0.23278415285706444
2212	23	0.2803514522178503
2212	24	0.1896143313859895
2212	25	0.34880584439036255
2213	1	-0.44752468942387935
2213	2	-0.28401647560027143
2213	3	-0.42427065617141935
2213	4	-0.2689346275212756
2213	5	-0.2509022288668845
2213	6	-0.03361705860410069
2213	7	-0.38226527438300195
2213	8	-0.06328125554289699
2213	9	-0.5213099794062259
2213	10	-0.22868191657310177
2213	11	-0.38554974880897674
2213	12	-0.2517161290116334
2213	13	-0.3381851568083576
2213	14	-0.40247843893359203
2213	15	-0.3381188262883654
2213	16	-0.32400573361181984
2213	17	-0.3218920468955062
2213	18	-0.2778841226826998
2213	19	-0.3481300273580672
2213	20	-0.14021138932978516
2213	21	-0.34736515050057193
2213	22	-0.5107437659246585
2213	23	-0.41168115960093843
2213	24	-0.11974660147485139
2213	25	0.13369673925994618
2214	1	-2.5971959668236018
2214	2	-1.3485563792144895
2214	3	-1.6685550567898453
2214	4	-3.310335110932023
2214	5	-1.8085136841867921
2214	6	-1.4389864413827596
2214	7	-1.3470285455392172
2214	8	-1.2351461450384278
2214	9	-1.0030930628291514
2214	10	-1.0018829774094158
2214	11	-1.177920326119299
2214	12	-1.0917723732591966
2214	13	-1.331541757851248
2214	14	-1.2492988936624059
2214	15	-1.6457444826221774
2214	16	-1.3620813274611763
2214	17	-3.595275765700613
2214	18	-3.3692266906541057
2214	19	-2.1182434996335715
2214	20	-1.8398265006904353
2214	21	-1.793818992677501
2214	22	-2.7276115655989805
2214	23	-1.6397034440789469
2214	24	-2.6684349834813013
2214	25	-2.5791668890209003
2215	1	-1.7352844369538722
2215	2	-3.4164840912311156
2215	3	-1.9883617834744918
2215	4	-3.4393182540228935
2215	5	-2.2016154421405716
2215	6	-2.146462549601239
2215	7	-2.3007009454373915
2215	8	-1.631276046496378
2215	9	-3.91925743223322
2215	10	-2.2768459632781166
2215	11	-1.8494920836088262
2215	12	-1.7619884299651645
2215	13	-1.9795666294534158
2215	14	-1.751637194433016
2215	15	-1.881104535442013
2215	16	-1.9368242409908376
2215	17	-2.1943817649287816
2215	18	-1.8685482741002701
2215	19	-2.00256804719546
2215	20	-1.941530102911969
2215	21	-2.546121548034513
2215	22	-2.2579489477459163
2215	23	-2.211956170998498
2215	24	-2.1664978342637298
2215	25	-2.9905047268768215
2216	1	-3.5928718281421723
2216	2	-3.66806677702143
2216	3	-4.915894819899197
2216	4	-5.441955819809415
2216	5	-5.252136360878861
2216	6	-4.331878742204694
2216	7	-5.820082634143475
2216	8	-3.6668618927641745
2216	9	-4.410825708183673
2216	10	-3.353754850809217
2216	11	-3.7980063716007777
2216	12	-4.1728850136323
2216	13	-3.828471688487845
2216	14	-4.52692825453002
2216	15	-4.088718440533842
2216	16	-4.325575786794296
2216	17	-4.1026569787428535
2216	18	-3.7673210663447882
2216	19	-4.39294382816993
2216	20	-4.938839963409986
2216	21	-4.538134581472306
2216	22	-4.428106149209704
2216	23	-4.331129784846073
2216	24	-4.687693164789433
2216	25	-4.772030363626758
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110937.0
3	3.0
4	1026.0
5	6044.0
6	7262.0
7	6672.0
8	5658.0
9	5327.0
10	4773.0
11	4497.0
12	4203.0
13	3991.0
14	3970.0
15	4065.0
16	4165.0
17	4513.0
18	5059.0
19	6132.0
20	7940.0
21	10211.0
22	12816.0
23	15436.0
24	17898.0
25	20253.0
26	22141.0
27	24637.0
28	27866.0
29	32424.0
30	39368.0
31	48984.0
32	60482.0
33	77203.0
34	99526.0
35	130357.0
36	176143.0
37	269046.0
38	746217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.48800444651878	18.583765949980624	12.700196044756213	24.228033558744382
2	14.712506111196896	20.007034968132974	42.28346382886774	22.99699509180239
3	19.476647814950606	25.696924789151517	28.69127914089849	26.13514825499939
4	12.234651616547566	16.419890429476	38.03167727755975	33.313780676416684
5	13.035449642523783	38.96984096600473	34.61579446942952	13.378914922041965
6	31.553491761251294	36.11043778889431	18.767176916002136	13.568893533852256
7	28.577550117691665	31.642173899306485	21.64262807854536	18.137647904456486
8	25.575565146113355	36.717708405344816	20.705778365940134	17.000948082601695
9	27.930375487953647	14.559692646593444	20.072069278927067	37.43786258652584
10	16.57000296730484	28.542312730231505	33.186860714908164	21.70082358755549
11	34.43849691827961	22.24086789777496	24.82384526821138	18.496789915734055
12	24.60273531603175	24.500774458138526	31.001779007123435	19.894711218706288
13	29.658376967227923	22.123001669656777	25.66334545504812	22.555275908067177
14	21.311380713351532	22.93644927826022	27.31403789670755	28.438132111680698
15	23.443449858085895	31.473828215061705	23.147723474602337	21.934998452250063
16	21.902710900923182	27.751471373085707	28.269667226037758	22.076150499953346
17	21.558287400709737	28.793150866881064	27.571967693849004	22.076594038560195
18	21.37381135079138	26.810415574180897	30.61340175133493	21.202371323692795
19	23.785146793579507	26.404674405663876	27.647584809761582	22.162593990995035
20	24.437528760489556	26.640557573315792	28.128153779004894	20.793759887189758
21	23.686891974482798	26.23273733297657	26.95197604281961	23.128394649721024
22	23.995469087235378	28.143357763854315	26.884469131181675	20.97670401772863
23	22.473896756221105	27.655588762878807	28.015531349814328	21.85498313108576
24	23.60416254196091	26.351228919564907	28.55069894939116	21.493909589083028
25	22.791725295613322	27.270433730775878	28.236638771630997	21.701202201979807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	295.0
1	295.0
2	683.5
3	1072.0
4	1072.0
5	1072.0
6	1812.5
7	2553.0
8	2553.0
9	2553.0
10	2893.5
11	3234.0
12	3234.0
13	3234.0
14	4842.5
15	6451.0
16	6451.0
17	6451.0
18	12657.0
19	18863.0
20	18863.0
21	18863.0
22	32017.5
23	45172.0
24	45172.0
25	45172.0
26	70189.0
27	95206.0
28	95206.0
29	95206.0
30	124887.0
31	154568.0
32	154568.0
33	154568.0
34	173583.0
35	192598.0
36	192598.0
37	192598.0
38	205610.5
39	218623.0
40	218623.0
41	218623.0
42	232298.0
43	245973.0
44	245973.0
45	245973.0
46	261371.0
47	276769.0
48	276769.0
49	276769.0
50	265127.0
51	253485.0
52	253485.0
53	253485.0
54	223934.5
55	194384.0
56	194384.0
57	194384.0
58	166032.0
59	137680.0
60	137680.0
61	137680.0
62	115522.5
63	93365.0
64	93365.0
65	93365.0
66	71046.5
67	48728.0
68	48728.0
69	48728.0
70	36167.5
71	23607.0
72	23607.0
73	23607.0
74	16585.0
75	9563.0
76	9563.0
77	9563.0
78	6552.5
79	3542.0
80	3542.0
81	3542.0
82	2314.5
83	1087.0
84	1087.0
85	1087.0
86	706.0
87	325.0
88	325.0
89	325.0
90	202.5
91	80.0
92	80.0
93	80.0
94	47.0
95	14.0
96	14.0
97	14.0
98	11.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3348386603493904
2	0.011246790595118005
3	0.009717621698413365
4	0.01183872694222948
5	0.012677303433970735
6	0.08060199926501237
7	0.07956611065756729
8	0.10383550088913772
9	0.08356168100056974
10	0.09066491716590742
11	0.08647203470720116
12	0.09658428063702217
13	0.08282176056668039
14	0.08839582783531344
15	0.08504152186834843
16	0.08662001879397901
17	0.0984587457362085
18	0.10097447521143225
19	0.09461115947998391
20	0.09357527087253883
21	0.0804540151782345
22	0.10092514718250631
23	0.09480847159568774
24	0.09115819745516698
25	0.0885438119220913
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2027245.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.71310574514882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53467798887189	39.289543625080206
2	13.893264218083221	14.647142117462266
3	5.008952507366798	7.921123295797633
4	2.337820147121499	4.929350425134199
5	1.2392744998299532	3.2663003878401375
6	0.7318685119621617	2.3147437357563696
7	0.49726677868727703	1.8348733401944366
8	0.3209649893886218	1.3535249140906394
9	0.2415968296262902	1.1461787305005007
>10	1.0722180952498872	9.653717664917218
>50	0.06494579316823179	2.4230474855084405
>100	0.04801904915842968	5.05869918350636
>500	0.005365399120623792	2.0309945041654176
>1k	0.0035769327470825244	3.5269004533391426
>5k	1.8825961826750127E-4	0.6038601367070535
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6651	0.32808072038653446	No Hit
GTCCTACAGTGGACATTTCTAAATT	5519	0.27224139164235206	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4855	0.23948758043551718	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4854	0.23943825240659122	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4282	0.21122261986094426	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3862	0.1905048477120427	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3687	0.18187244265000038	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3619	0.17851813668303534	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2828	0.13949966580260403	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2365	0.11666078840988632	No Hit
GGATACCACGTGTCCCGCCCTACTC	2158	0.10644988642221341	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2088	0.10299692439739647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.932802892595616E-5
3	0.0	0.0	0.0	0.0	4.932802892595616E-5
4	0.0	0.0	0.0	0.0	4.932802892595616E-5
5	0.0	0.0	0.0	0.0	4.932802892595616E-5
6	2.466401446297808E-4	0.0	0.0	0.0	4.932802892595616E-5
7	2.466401446297808E-4	0.0	0.0	0.0	4.932802892595616E-5
8	2.466401446297808E-4	0.0	0.0	0.0	4.932802892595616E-5
9	2.466401446297808E-4	0.0	0.0	0.0	9.865605785191232E-5
10	2.466401446297808E-4	0.0	0.0	0.0	9.865605785191232E-5
11	2.9596817355573697E-4	0.0	0.0	0.0	9.865605785191232E-5
12	2.9596817355573697E-4	0.0	0.0	0.0	9.865605785191232E-5
13	2.9596817355573697E-4	0.0	0.0	0.0	9.865605785191232E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	40	0.005285481	14.2477045	9
GGTATCA	1820	0.0	12.976931	1
TAGGACC	815	0.0	12.120447	4
CACCGTA	55	0.0030721363	12.089259	7
GCGCCAC	115	7.088056E-8	11.563925	13
CCAACGA	150	1.7644197E-10	11.398444	19
TGCGTTT	60	0.005881592	11.082094	12
GTCCTAA	1315	0.0	11.080448	1
GTCCTAG	280	0.0	10.883877	1
GGCGTGC	175	2.0008883E-11	10.855661	8
GGCGAGG	1245	0.0	10.757568	19
CGTGCGC	125	2.2457971E-7	10.638287	10
GTATCAA	3935	0.0	10.576169	1
TCCAACG	225	0.0	10.554636	18
GTCCTAC	2630	0.0	10.53729	1
TAGGACA	760	0.0	10.498049	4
CTAGGAC	355	0.0	10.43468	3
GTAGGAC	2625	0.0	10.348553	3
TGTAGGA	2760	0.0	10.289754	2
GTATAAT	315	0.0	10.279218	1
>>END_MODULE