FastQCFastQC Report
Thu 26 May 2016
SRR1512901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2027245
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81150.4002969547341343No Hit
TATCAACGCAGAGTACTTTTTTTTT60120.2965601099028485No Hit
GTCCTACAGTGGACATTTCTAAATT51690.2549765815182674No Hit
CTGTAGGACGTGGAATATGGCAAGA47840.2359852903817743No Hit
GGTATCAACGCAGAGTACTTTTTTT47450.234061497253662No Hit
CTTTAGGACGTGAAATATGGCGAGG46170.2277475095511396No Hit
GTCCTAAAGTGTGTATTTCTCATTT45850.22616901262550898No Hit
GTACTGGTTCACTATCGGTCAGTCA31240.15410076236468706No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28500.14058488243897507No Hit
GTCCTACAGTGTGCATTTCTCATTT26780.1321004614637106No Hit
CTGAAGGACCTGGAATATGGCGAGA24390.12031106255040708No Hit
CTGTAGGACCTGGAATATGGCGAGA20540.10131977141391395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC307.6640316E-419.021973
GGTATCA18650.013.1075861
GTGCGCC957.4633135E-812.99031311
CTACGAG550.003042547712.1048913
CCCGTAC3000.011.39122416
TAGGACT3850.011.3626524
GTAGAAC7300.011.3350113
CGAATGG1451.2350938E-911.12853219
TAGGACC6800.011.0484364
CCCCGTA3100.011.02403715
TGTAGAA7950.011.0064242
AATGTCC7300.010.932268
TGTCCAC8000.010.92197810
GGCGAGG13150.010.82735819
GAAATGT7400.010.7949196
AAATGTC7250.010.7514077
TCCAACG1951.8189894E-1210.70943318
CCGTACA3200.010.67927217
TAGAAAT7400.010.6666734
ATAATAC1252.2054337E-710.6523033
Produced by FastQC (version 0.11.5)