##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512901_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2027245 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.09518089821408 31.0 30.0 34.0 26.0 34.0 2 30.116353474789676 31.0 30.0 34.0 26.0 34.0 3 30.316782135361045 31.0 30.0 34.0 26.0 34.0 4 33.90509237906617 37.0 35.0 37.0 30.0 37.0 5 33.4612442008736 37.0 35.0 37.0 28.0 37.0 6 33.32029133133884 37.0 35.0 37.0 27.0 37.0 7 33.160446813286015 37.0 35.0 37.0 26.0 37.0 8 33.10229301342462 37.0 35.0 37.0 26.0 37.0 9 34.40682897232451 39.0 35.0 39.0 26.0 39.0 10 34.328981943475014 39.0 35.0 39.0 26.0 39.0 11 34.31230512345572 39.0 35.0 39.0 25.0 39.0 12 34.168390599064246 39.0 35.0 39.0 25.0 39.0 13 34.154188072975884 39.0 35.0 39.0 25.0 39.0 14 35.22706924915341 39.0 35.0 41.0 25.0 41.0 15 35.229463138397186 40.0 35.0 41.0 25.0 41.0 16 35.226066903605634 40.0 35.0 41.0 25.0 41.0 17 35.22254784202205 40.0 36.0 41.0 25.0 41.0 18 35.15162498859289 40.0 35.0 41.0 25.0 41.0 19 35.15950514121381 40.0 35.0 41.0 24.0 41.0 20 35.13573939015758 40.0 35.0 41.0 24.0 41.0 21 35.10277050874463 40.0 35.0 41.0 24.0 41.0 22 35.062673233871585 40.0 35.0 41.0 24.0 41.0 23 34.897887033880956 40.0 35.0 41.0 22.0 41.0 24 34.85923161729342 40.0 35.0 41.0 22.0 41.0 25 34.79687112312523 40.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6138416103978095 1101 2 0.5172867069691875 1101 3 0.5491307234059022 1101 4 0.5678576953120285 1101 5 0.5862983256576086 1101 6 0.6227105838766747 1101 7 0.7973400553778376 1101 8 0.5339158399852124 1101 9 0.8483311566453935 1101 10 0.48262832315215576 1101 11 0.9014951415564241 1101 12 0.9736533009181301 1101 13 0.9214223536281594 1101 14 1.1055851197854025 1101 15 0.7943185916027105 1101 16 0.9455103132989748 1101 17 1.1531556474408902 1101 18 0.9596611032945646 1101 19 0.8322794961772644 1101 20 0.8604585038091912 1101 21 1.0372206475392716 1101 22 0.8667098922821808 1101 23 1.0637877321779499 1101 24 1.102208262400893 1101 25 1.1826684581711717 1102 1 0.820068055788699 1102 2 0.9131425420724888 1102 3 0.5302458638829286 1102 4 0.6394804926106659 1102 5 0.8879821750461971 1102 6 0.8333890146824601 1102 7 0.7520633710982523 1102 8 0.8643750698131711 1102 9 0.9366808605167307 1102 10 0.9068505611629334 1102 11 0.9867845871173841 1102 12 0.8539033282871102 1102 13 0.997159735156373 1102 14 0.9881191504848985 1102 15 1.195998925515866 1102 16 1.031596494387344 1102 17 1.097292420337908 1102 18 0.45157577373247193 1102 19 0.8619678445298433 1102 20 1.114343711184766 1102 21 0.9950862493300718 1102 22 0.8780606551245143 1102 23 1.1255626587726084 1102 24 1.124746893833695 1102 25 1.092177586952964 1103 1 0.6218501280458817 1103 2 0.8268981646064724 1103 3 0.6252605799256763 1103 4 0.5620609856868768 1103 5 0.519168024739713 1103 6 0.6240959381478035 1103 7 0.5170840188833736 1103 8 0.6795750407564327 1103 9 0.7394266006242631 1103 10 0.6597084560206525 1103 11 0.7500445302726177 1103 12 0.8459373109083046 1103 13 0.8279778026588573 1103 14 0.8609373533561495 1103 15 0.9609057542635426 1103 16 0.848458729139665 1103 17 0.8289195742620166 1103 18 0.8877572370932114 1103 19 0.7876356157638966 1103 20 0.6430265638965551 1103 21 0.8258549446634191 1103 22 0.7975613568062343 1103 23 0.7610040252242811 1103 24 0.8389927172567653 1103 25 0.762154886884133 1104 1 0.6420831302437122 1104 2 0.7949413270267911 1104 3 0.6747840474114 1104 4 0.561514073001085 1104 5 0.6196506302183025 1104 6 0.5747953272046331 1104 7 0.5315681129053118 1104 8 0.6396790747365699 1104 9 0.6028925268471852 1104 10 0.8117907020430124 1104 11 0.873555875746014 1104 12 0.9393021452527606 1104 13 0.7223324363009809 1104 14 0.8929679164261799 1104 15 0.994753957036707 1104 16 1.0130377422597334 1104 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-3.173369357135453 2216 22 -2.530375969267446 2216 23 -3.7188598789226575 2216 24 -2.92829240425775 2216 25 -3.064692939911719 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62886.0 3 1.0 4 1106.0 5 8982.0 6 11033.0 7 8251.0 8 6660.0 9 5628.0 10 4651.0 11 4335.0 12 3889.0 13 3454.0 14 3440.0 15 3333.0 16 3467.0 17 3603.0 18 4028.0 19 4849.0 20 6498.0 21 8501.0 22 10672.0 23 13035.0 24 15323.0 25 17287.0 26 19421.0 27 21022.0 28 23611.0 29 28330.0 30 35626.0 31 45103.0 32 56968.0 33 73765.0 34 95595.0 35 126031.0 36 172679.0 37 268603.0 38 845579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.19299573397079 19.629214376237016 13.73532055943683 26.442469330355355 2 13.230096574730682 20.43609141289275 40.956223074303814 25.377588938072748 3 17.26331733164929 25.638052761153894 29.71034156112627 27.388288346070546 4 11.492796190855804 17.033656583076894 37.21423772224859 34.25930950381871 5 12.623997584433807 39.078295723322945 34.78291973669539 13.514786955547859 6 31.613060529872055 36.818201112101534 18.164045340890805 13.404693017135601 7 28.12554920371632 31.829922099463882 21.740914072450458 18.30361462436934 8 25.560190182586535 37.07178440050679 20.312005658292257 17.05601975861441 9 27.17349310678476 14.405317404671528 20.135362704846248 38.285826783697466 10 15.980395205106603 29.068979523146083 33.19766325690109 21.752962014846226 11 34.26304451099764 22.19753472542532 24.729505006371117 18.80991575720592 12 24.134673677179972 24.802823147321902 31.05417658778606 20.008326587712066 13 29.310109232334902 22.134753917698124 25.81268156675367 22.742455283213303 14 21.01059867780434 22.798897413914723 27.40680158284638 28.783702325434557 15 23.231792509797884 31.5394224279811 23.034453429950545 22.194331632270465 16 21.8147913362423 27.779178270060406 28.128797053868524 22.27723333982877 17 21.191318910892797 28.856753150335567 27.75033112746126 22.201596811310374 18 21.08719674023501 26.772201229318153 30.809860197125015 21.330741833321824 19 23.520869209622433 26.348286644623354 27.96749836352327 22.163345782230945 20 24.187606673178045 26.536932350706877 28.412851096575608 20.862609879539466 21 23.22917221607266 26.12793526580915 27.263023099340778 23.379869418777414 22 23.618945392176645 27.973986268009117 27.210779992669824 21.196288347144414 23 22.144190761353464 27.514582598551236 28.306840071131017 22.034386568964283 24 23.48260817020143 26.363217075390494 28.704078688071743 21.450096066336332 25 22.56500817612079 27.141712027899935 28.46133545772711 21.83194433825216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 103.0 2 357.5 3 612.0 4 612.0 5 612.0 6 1468.5 7 2325.0 8 2325.0 9 2325.0 10 2762.5 11 3200.0 12 3200.0 13 3200.0 14 4761.5 15 6323.0 16 6323.0 17 6323.0 18 12892.5 19 19462.0 20 19462.0 21 19462.0 22 33278.0 23 47094.0 24 47094.0 25 47094.0 26 73334.5 27 99575.0 28 99575.0 29 99575.0 30 127824.0 31 156073.0 32 156073.0 33 156073.0 34 175066.0 35 194059.0 36 194059.0 37 194059.0 38 206674.0 39 219289.0 40 219289.0 41 219289.0 42 232718.5 43 246148.0 44 246148.0 45 246148.0 46 261830.0 47 277512.0 48 277512.0 49 277512.0 50 265317.0 51 253122.0 52 253122.0 53 253122.0 54 222709.0 55 192296.0 56 192296.0 57 192296.0 58 164005.0 59 135714.0 60 135714.0 61 135714.0 62 113412.0 63 91110.0 64 91110.0 65 91110.0 66 69097.5 67 47085.0 68 47085.0 69 47085.0 70 34745.5 71 22406.0 72 22406.0 73 22406.0 74 15686.5 75 8967.0 76 8967.0 77 8967.0 78 6191.0 79 3415.0 80 3415.0 81 3415.0 82 2207.0 83 999.0 84 999.0 85 999.0 86 641.5 87 284.0 88 284.0 89 284.0 90 174.5 91 65.0 92 65.0 93 65.0 94 35.0 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08425227340553312 2 0.041188904153173396 3 0.01849801084723356 4 0.006067347557892608 5 0.01928725931004886 6 0.05776312187229467 7 0.07818492584764052 8 0.05879901047973975 9 0.02673579167786824 10 0.0496239970995119 11 0.006313987702522388 12 1.9731211570382464E-4 13 0.004834146834743704 14 5.426083181855177E-4 15 0.013663864012489858 16 0.008879045206672109 17 0.003946242314076493 18 0.005179443037225397 19 0.0011838726942229479 20 0.001233200723148904 21 4.932802892595616E-5 22 3.946242314076493E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2027245.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.187306073196595 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03999218299431 37.38720435454817 2 14.593464096666455 14.940002267685426 3 5.37794722569533 8.25847892061496 4 2.4588427817684204 5.034461522250011 5 1.3597690747449678 3.4801457908919002 6 0.799316060976225 2.4548901517447144 7 0.5269477246407741 1.8881124126033277 8 0.34430577292570486 1.4099267985213266 9 0.24994143672623811 1.1514445939872406 >10 1.1219122584095478 9.837423141860342 >50 0.0656546828388431 2.3469463272635975 >100 0.052347735971742575 5.27399586963195 >500 0.005213981258955697 1.8754929114198335 >1k 0.0040553187569655385 3.7077892380086923 >5k 2.8966562549753846E-4 0.9536856989684848 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8115 0.4002969547341343 No Hit TATCAACGCAGAGTACTTTTTTTTT 6012 0.2965601099028485 No Hit GTCCTACAGTGGACATTTCTAAATT 5169 0.2549765815182674 No Hit CTGTAGGACGTGGAATATGGCAAGA 4784 0.2359852903817743 No Hit GGTATCAACGCAGAGTACTTTTTTT 4745 0.234061497253662 No Hit CTTTAGGACGTGAAATATGGCGAGG 4617 0.2277475095511396 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4585 0.22616901262550898 No Hit GTACTGGTTCACTATCGGTCAGTCA 3124 0.15410076236468706 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2850 0.14058488243897507 No Hit GTCCTACAGTGTGCATTTCTCATTT 2678 0.1321004614637106 No Hit CTGAAGGACCTGGAATATGGCGAGA 2439 0.12031106255040708 No Hit CTGTAGGACCTGGAATATGGCGAGA 2054 0.10131977141391395 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.932802892595616E-5 1.4798408677786849E-4 7 0.0 0.0 0.0 4.932802892595616E-5 1.4798408677786849E-4 8 0.0 0.0 0.0 9.865605785191232E-5 1.4798408677786849E-4 9 0.0 0.0 0.0 9.865605785191232E-5 1.4798408677786849E-4 10 0.0 0.0 0.0 9.865605785191232E-5 1.4798408677786849E-4 11 0.0 0.0 0.0 1.9731211570382464E-4 1.4798408677786849E-4 12 0.0 0.0 0.0 1.9731211570382464E-4 1.4798408677786849E-4 13 0.0 0.0 0.0 1.9731211570382464E-4 1.4798408677786849E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGAC 30 7.6640316E-4 19.02197 3 GGTATCA 1865 0.0 13.107586 1 GTGCGCC 95 7.4633135E-8 12.990313 11 CTACGAG 55 0.0030425477 12.104891 3 CCCGTAC 300 0.0 11.391224 16 TAGGACT 385 0.0 11.362652 4 GTAGAAC 730 0.0 11.335011 3 CGAATGG 145 1.2350938E-9 11.128532 19 TAGGACC 680 0.0 11.048436 4 CCCCGTA 310 0.0 11.024037 15 TGTAGAA 795 0.0 11.006424 2 AATGTCC 730 0.0 10.93226 8 TGTCCAC 800 0.0 10.921978 10 GGCGAGG 1315 0.0 10.827358 19 GAAATGT 740 0.0 10.794919 6 AAATGTC 725 0.0 10.751407 7 TCCAACG 195 1.8189894E-12 10.709433 18 CCGTACA 320 0.0 10.679272 17 TAGAAAT 740 0.0 10.666673 4 ATAATAC 125 2.2054337E-7 10.652303 3 >>END_MODULE