FastQCFastQC Report
Thu 26 May 2016
SRR1512900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202987
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74260.33708778127151906No Hit
GTCCTACAGTGGACATTTCTAAATT54890.24916170635596127No Hit
GTCCTAAAGTGTGTATTTCTCATTT49130.22301538774400392No Hit
CTGTAGGACGTGGAATATGGCAAGA46860.21271119620769438No Hit
GGTATCAACGCAGAGTACTTTTTTT43680.1982762494740096No Hit
GTACTGGTTCACTATCGGTCAGTCA42730.19396392261960693No Hit
TATCAACGCAGAGTACTTTTTTTTT41820.18983316742223172No Hit
CTTTAGGACGTGAAATATGGCGAGG37260.16913399852109887No Hit
GTCCTACAGTGTGCATTTCTCATTT28780.1306408072312728No Hit
GGATACCACGTGTCCCGCCCTACTC24850.11280139192832277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7900.013.2259344
GGTATCA21700.012.9905951
TTAGGAC14250.011.7316363
GGCGAGG11050.011.34703219
GCCCGTC600.005884303711.0814777
GACGTGA13300.010.9981587
GTCCTAC26700.010.9859221
GTATCAA47350.010.8610261
TGAAACG700.00149363110.8555725
GTCCTAA13200.010.8221711
TGTAGGA24850.010.74092
TCCTACA28850.010.6674342
ACGTGAA14900.010.6458688
AGGACCT16000.010.6265875
TATTAGG1701.4006218E-1010.6161112
TGGCGAG24050.010.58570918
TAGGACG26300.010.5821194
GGACCTG15850.010.547136
ATTTCTA18300.010.53696515
AGGACGT26350.010.52599055
Produced by FastQC (version 0.11.5)