##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512900_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2202987
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.78960111884455	31.0	30.0	34.0	22.0	34.0
2	28.963445086148944	31.0	30.0	34.0	20.0	34.0
3	29.070356747452436	31.0	30.0	34.0	22.0	34.0
4	32.47244672801065	35.0	33.0	37.0	23.0	37.0
5	32.63760067580971	35.0	33.0	37.0	25.0	37.0
6	32.56823440174636	36.0	33.0	37.0	25.0	37.0
7	32.50565482229355	36.0	33.0	37.0	23.0	37.0
8	32.42181819502339	37.0	33.0	37.0	22.0	37.0
9	33.691394456708096	38.0	34.0	39.0	21.0	39.0
10	33.56097925226068	38.0	34.0	39.0	19.0	39.0
11	33.61041077409899	38.0	34.0	39.0	19.0	39.0
12	33.5095091346431	38.0	34.0	39.0	18.0	39.0
13	33.48167556140822	38.0	34.0	39.0	18.0	39.0
14	34.321975572257124	39.0	34.0	41.0	17.0	41.0
15	34.496659762404406	39.0	34.0	41.0	17.0	41.0
16	34.430210437011205	39.0	34.0	41.0	17.0	41.0
17	34.20303978189612	39.0	33.0	41.0	17.0	41.0
18	34.20062487885766	39.0	33.0	41.0	17.0	41.0
19	34.31517889120544	39.0	34.0	41.0	18.0	41.0
20	34.285786071365834	39.0	34.0	41.0	17.0	41.0
21	34.185947080032705	39.0	34.0	41.0	15.0	41.0
22	34.19206559094538	39.0	34.0	41.0	11.0	41.0
23	33.859480786768145	39.0	34.0	41.0	8.0	41.0
24	33.91949294299059	39.0	34.0	41.0	2.0	41.0
25	33.62768413976115	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7166217530627748
1101	2	0.2965435306814008
1101	3	0.6628208435797909
1101	4	0.8355221411381493
1101	5	0.7131028663038634
1101	6	0.6701030700805148
1101	7	0.35604971468912083
1101	8	0.6574804757210444
1101	9	0.6982487604223309
1101	10	0.711313512218517
1101	11	0.6848675964853186
1101	12	0.5429248509102251
1101	13	0.9247577132514664
1101	14	1.001520788526797
1101	15	0.9166659358437386
1101	16	0.7610601183458812
1101	17	0.17156823080829042
1101	18	0.6230186945695877
1101	19	0.11030973087851947
1101	20	-0.5484091865963094
1101	21	0.6180029776519262
1101	22	0.6453964034100963
1101	23	0.2518452463038585
1101	24	0.7613913496215403
1101	25	0.3671978184403244
1102	1	0.8302425782304539
1102	2	0.8236876758903726
1102	3	0.7648465515008418
1102	4	0.9339359585999603
1102	5	0.7424375501497309
1102	6	0.6688386605475003
1102	7	0.6410331685623021
1102	8	0.6188312735262329
1102	9	0.9762957912733867
1102	10	0.9431249706494214
1102	11	0.7509066853346624
1102	12	1.0071373559013708
1102	13	0.8450455547161795
1102	14	1.0906695751224191
1102	15	0.9771204187965168
1102	16	0.902419416865591
1102	17	1.0757383731226398
1102	18	1.0555170288243971
1102	19	0.7575872191965587
1102	20	1.086788572429569
1102	21	1.2378476147873485
1102	22	1.0215170144074293
1102	23	0.9085340536181263
1102	24	0.26962466649093386
1102	25	0.6549350028565186
1103	1	0.4903306471331774
1103	2	0.6445515188078623
1103	3	0.7815956788083867
1103	4	0.9853827835347673
1103	5	0.5647052071117571
1103	6	0.2891466244925667
1103	7	0.12271436766413046
1103	8	0.4010135484334185
1103	9	0.4632206085783963
1103	10	0.6984685353288853
1103	11	0.4450109307675518
1103	12	0.591081605940289
1103	13	0.7511518216822424
1103	14	0.8022704033539441
1103	15	0.7275224863899297
1103	16	0.6949360763886574
1103	17	0.35851307666309395
1103	18	0.8326042724809781
1103	19	0.776256973605868
1103	20	0.9417760657924106
1103	21	0.6478390211389708
1103	22	0.6923599809433796
1103	23	0.15043020055032486
1103	24	0.3536133134924313
1103	25	0.5855874590832855
1104	1	0.9577668896318379
1104	2	0.9541798162443342
1104	3	0.9324339475728429
1104	4	1.0900079698408263
1104	5	0.7997593159744483
1104	6	0.723768689332239
1104	7	0.9596221727771308
1104	8	0.6572373182965947
1104	9	0.9702762388975472
1104	10	1.0002737351544155
1104	11	0.7717450293769517
1104	12	1.0545712399845328
1104	13	0.9969441883532468
1104	14	1.324759969180704
1104	15	1.0331562164182344
1104	16	1.0423107164381022
1104	17	1.3708902903719107
1104	18	1.0711742687807018
1104	19	0.7425691161101113
1104	20	0.9219448730408288
1104	21	0.9936988197527086
1104	22	0.6970782324260725
1104	23	1.029659053649155
1104	24	0.891176709234422
1104	25	0.5040349119873895
1105	1	0.5018218198152162
1105	2	0.6181859154604687
1105	3	0.6931134555197573
1105	4	0.833913163484084
1105	5	0.5940815278418512
1105	6	0.7550504458751348
1105	7	0.68934845281386
1105	8	0.7515941224406077
1105	9	0.34600275020275717
1105	10	0.4464881347530323
1105	11	0.5483139557130414
1105	12	0.7054371738437055
1105	13	0.7809781377818865
1105	14	0.8483797324441014
1105	15	0.7958514839400053
1105	16	0.7088828178160114
1105	17	1.0699210696584913
1105	18	0.8742268657003223
1105	19	0.622275209152555
1105	20	0.9100220520357709
1105	21	0.7587422952867584
1105	22	0.752726965006218
1105	23	0.9235634143339055
1105	24	0.875845863017183
1105	25	1.2329064296083772
1106	1	0.40669629265023133
1106	2	0.40369874305645936
1106	3	0.37883292019364845
1106	4	0.29179811243356824
1106	5	0.18922410708456283
1106	6	0.365505449875549
1106	7	0.3275969793438236
1106	8	0.31591200181694035
1106	9	0.43528197251887946
1106	10	0.28358622580703496
1106	11	0.06468719022617364
1106	12	0.32390682489680955
1106	13	0.4160135413056665
1106	14	0.6594829541272489
1106	15	0.4767936385177691
1106	16	0.4795702279941949
1106	17	0.7035269975063727
1106	18	0.4930575092389162
1106	19	0.596484282631323
1106	20	0.6842724938551044
1106	21	0.7413894189081844
1106	22	0.6020723698464181
1106	23	0.8842486554878022
1106	24	0.49669359683015557
1106	25	0.6533277932698596
1107	1	0.5936826104026771
1107	2	0.588304585621124
1107	3	0.657908376687832
1107	4	0.629789628763767
1107	5	0.560179456060645
1107	6	0.6216801940086967
1107	7	0.4489569233248787
1107	8	0.4447497159603202
1107	9	0.4530370578958056
1107	10	0.7236517297012242
1107	11	0.6233949110585044
1107	12	0.6781927441988529
1107	13	0.3785270367409268
1107	14	0.8231269732060227
1107	15	0.5401032924655311
1107	16	0.6594443173358329
1107	17	0.8617278095831082
1107	18	0.9276031017472874
1107	19	0.7750088696854363
1107	20	0.8334846243647291
1107	21	0.5167502388038159
1107	22	0.6738489505959251
1107	23	0.9077079902051111
1107	24	0.7632231028621703
1107	25	0.9805298895264372
1108	1	1.0406910332670165
1108	2	1.0132650326748411
1108	3	1.0409521496562633
1108	4	1.2253134690776335
1108	5	0.9589467941512666
1108	6	1.1119603991061453
1108	7	1.0755177614568865
1108	8	1.1236142301345637
1108	9	1.1399166467013444
1108	10	1.2377364831141762
1108	11	0.9027472579187119
1108	12	1.0771750660231731
1108	13	1.1654095929935764
1108	14	1.2986332743456757
1108	15	1.2875816652424845
1108	16	1.0440857482075927
1108	17	1.2299937740580802
1108	18	1.37313287695536
1108	19	1.3440433248699577
1108	20	1.4124454243884657
1108	21	1.3254546201613806
1108	22	1.4086862329766277
1108	23	1.6660440176680495
1108	24	1.5747067327059412
1108	25	1.8404353841452021
1109	1	0.9715704891546402
1109	2	0.9210073406477761
1109	3	1.0482936379076584
1109	4	1.0199097784273405
1109	5	0.923392165611375
1109	6	0.9992014207126587
1109	7	0.7996900298712788
1109	8	0.7433708197884386
1109	9	0.9789547745385647
1109	10	0.9922089459903702
1109	11	0.9497527681985432
1109	12	0.880787360577294
1109	13	0.9780900570371713
1109	14	1.041648828117701
1109	15	0.8579416257791053
1109	16	0.5560373770234648
1109	17	0.9141037385600796
1109	18	0.9324359550351033
1109	19	0.9190600587364486
1109	20	0.8976301631022281
1109	21	0.8818420401005369
1109	22	0.9143181703935568
1109	23	1.2871304342096792
1109	24	1.103298803866906
1109	25	0.7097308804352025
1110	1	0.5915093819946122
1110	2	0.5971981952341885
1110	3	0.5073343587973298
1110	4	0.547002341074105
1110	5	0.39543544124121865
1110	6	0.45660559809029877
1110	7	0.356552268586519
1110	8	0.3282771923355128
1110	9	0.5215811904725172
1110	10	0.4717845333223565
1110	11	0.3578631742949554
1110	12	0.44322234979016883
1110	13	0.530989865928305
1110	14	0.49306267616032073
1110	15	0.42397113044557955
1110	16	0.43909225824592824
1110	17	0.6302232352405
1110	18	0.03565460980348689
1110	19	0.31046731133787375
1110	20	0.48050068982369964
1110	21	0.5847368825449806
1110	22	0.5307002927747462
1110	23	0.6577894618191493
1110	24	0.5574056579606506
1110	25	0.7481779321537942
1111	1	0.5017710487262654
1111	2	0.37259182570107185
1111	3	0.4526414155714136
1111	4	0.343138683316873
1111	5	0.5226689546853294
1111	6	0.4953704154804939
1111	7	0.35327955377399434
1111	8	0.5329638535433219
1111	9	0.5894683410081853
1111	10	0.5442392427287999
1111	11	0.3474205303018394
1111	12	0.05561387666716655
1111	13	0.3416935582976848
1111	14	0.4111036475218057
1111	15	0.44658647440281385
1111	16	0.4928433319983938
1111	17	0.0178141423442284
1111	18	0.46134203785074845
1111	19	0.5124677894801906
1111	20	0.5487148203864862
1111	21	0.6035367768337778
1111	22	0.307662497869444
1111	23	0.5609089268984135
1111	24	0.538823487785308
1111	25	0.7266681446120415
1112	1	0.3459386912601161
1112	2	0.25923890184876797
1112	3	0.27022636519337695
1112	4	0.4043737294948073
1112	5	0.419509820234083
1112	6	0.21534992010584375
1112	7	0.05581092295676626
1112	8	0.24649260265744743
1112	9	0.19158787496186847
1112	10	0.3409324122250297
1112	11	0.18503780451615626
1112	12	0.27182245424268103
1112	13	0.2819240635950635
1112	14	0.32742975404818253
1112	15	0.1433510016050974
1112	16	0.38490128776889065
1112	17	0.5934590939694573
1112	18	0.3489432253046658
1112	19	0.5236016059120416
1112	20	0.21210323425236766
1112	21	0.3465899440293114
1112	22	0.24693385614201446
1112	23	0.5569185133329455
1112	24	0.5669800788052513
1112	25	0.5300766022115653
1113	1	0.03785585199823416
1113	2	0.07087067324427565
1113	3	0.18671353270108426
1113	4	0.19484421804205
1113	5	0.03488446272492496
1113	6	-0.21688854567311466
1113	7	-0.2890121314394847
1113	8	-0.12739467265060256
1113	9	-0.24197732080805423
1113	10	-0.1541129430622874
1113	11	-0.13660381993705073
1113	12	-0.2623050830326221
1113	13	-0.08920462985947353
1113	14	0.1962903402603402
1113	15	-0.16420493747004627
1113	16	-0.2599073980891191
1113	17	-0.05654380636153178
1113	18	-0.28969021618129887
1113	19	-0.5851718590363504
1113	20	-0.4440769273706948
1113	21	-0.18786094586403834
1113	22	-0.6678210190965572
1113	23	-4.205961316071122
1113	24	-1.8692961801824453
1113	25	-0.9018986958428243
1114	1	-0.3251310498426534
1114	2	-0.5613229970849289
1114	3	-0.40900689034114635
1114	4	-0.2630147858676821
1114	5	-0.3364626321151647
1114	6	-0.903275945844559
1114	7	-0.7929594677845024
1114	8	-0.6118596457174874
1114	9	-0.8165062460937804
1114	10	-0.35084576433484926
1114	11	-0.4958441768521453
1114	12	-1.4253636781242491
1114	13	-0.5898824798288587
1114	14	-2.65567194582783
1114	15	-1.3068832685753975
1114	16	-1.2892699657971178
1114	17	-1.1454652124618931
1114	18	-1.035847266215704
1114	19	-1.1143872491065352
1114	20	-0.6921426019973325
1114	21	-0.5543998908541283
1114	22	-0.8122077038970588
1114	23	-5.064260091794349
1114	24	-2.3312726010495872
1114	25	-2.228825905244637
1115	1	-1.8346904486532125
1115	2	-1.7646318246929695
1115	3	-1.3737223790994406
1115	4	-1.451395043065979
1115	5	-1.4345447299634984
1115	6	-1.5999449128762002
1115	7	-2.149112668217903
1115	8	-2.3382076352343013
1115	9	-3.538128156603534
1115	10	-4.525760433942509
1115	11	-2.9816045713292283
1115	12	-3.2881591163930857
1115	13	-2.638211891858738
1115	14	-3.2341744980355216
1115	15	-2.8760541986066777
1115	16	-3.0682657904497894
1115	17	-2.927403555519394
1115	18	-5.642543142058688
1115	19	-3.6100258592446863
1115	20	-4.056012737442984
1115	21	-3.5557264013953365
1115	22	-3.049733301253827
1115	23	-3.508253541829383
1115	24	-3.2490283311683505
1115	25	-3.552762931476721
1116	1	-1.1384199453400434
1116	2	-0.9999034058295422
1116	3	-1.310714492771858
1116	4	-1.1224672496437371
1116	5	-1.0173817205591114
1116	6	-3.298902953666502
1116	7	-1.8883549280037073
1116	8	-2.2167502206442187
1116	9	-2.9535909375397225
1116	10	-2.4157607755062607
1116	11	-2.0183002835655586
1116	12	-1.7636591277785456
1116	13	-2.9791410970778323
1116	14	-6.06953545502126
1116	15	-4.058040126179982
1116	16	-2.6429959550835207
1116	17	-3.715763632029674
1116	18	-2.4945911962838316
1116	19	-2.194159653562579
1116	20	-2.939012160579754
1116	21	-3.5453755015645996
1116	22	-3.059253442129897
1116	23	-2.216716922399719
1116	24	-3.1426744862990184
1116	25	-3.038447994514211
1201	1	0.6045366371794287
1201	2	0.6700272953455837
1201	3	1.0107660189851906
1201	4	0.8598469719657587
1201	5	0.44865171819957084
1201	6	0.916045530795806
1201	7	0.9711719709076689
1201	8	0.9113591513991395
1201	9	1.3246380517285203
1201	10	1.2971091303832694
1201	11	1.4389200013532104
1201	12	1.2107437515306643
1201	13	1.3251469728659018
1201	14	1.4094370146737774
1201	15	1.4565101169162062
1201	16	1.3852957873696852
1201	17	1.6189733452362987
1201	18	1.3913141487428362
1201	19	1.601976970740857
1201	20	1.5286663770586415
1201	21	1.6911283157599115
1201	22	1.414286918787802
1201	23	1.8104581995345441
1201	24	1.6357164061864324
1201	25	1.6473406295884203
1202	1	0.7844956886442915
1202	2	0.6707520837190764
1202	3	0.7865409235675251
1202	4	0.954415790979759
1202	5	0.7416251123026854
1202	6	0.9679532817011847
1202	7	1.0077554501677426
1202	8	0.9444973104506431
1202	9	1.1706296891618209
1202	10	1.0652801301529635
1202	11	1.0867522974412225
1202	12	1.2491875675109014
1202	13	1.1034076518831313
1202	14	1.55647136258861
1202	15	1.1877545371719336
1202	16	0.94861987183765
1202	17	1.3171422025937147
1202	18	1.3197705869710816
1202	19	1.329659084950812
1202	20	1.217932612204713
1202	21	1.3390294065044301
1202	22	1.2305845824409474
1202	23	1.393501294497888
1202	24	1.4031773717665814
1202	25	1.663854664658679
1203	1	1.1641027866586704
1203	2	1.2466140141594906
1203	3	1.1477270656696987
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2211	19	0.12576806023305664
2211	20	0.10787821319740942
2211	21	0.276578016615332
2211	22	0.24503189517430712
2211	23	0.36729670548183435
2211	24	0.1280491297076196
2211	25	0.562723475525182
2212	1	-0.04768003822232103
2212	2	0.1766466328088825
2212	3	-0.18629950856484712
2212	4	-0.00901469988269099
2212	5	-0.1998787007597329
2212	6	-0.1984871216648827
2212	7	-0.09603122051535706
2212	8	0.15883667080056085
2212	9	0.19004253759288758
2212	10	-0.11738677804214603
2212	11	-0.31625469772448156
2212	12	0.12142869023821845
2212	13	0.1583246722770184
2212	14	0.24608272229535544
2212	15	-0.07661207993390917
2212	16	-0.26982021401349954
2212	17	0.13244418268593705
2212	18	0.3312866087534232
2212	19	0.14808583924617125
2212	20	0.25431520818089126
2212	21	0.3348438746686426
2212	22	0.383135913171067
2212	23	0.38878792803581064
2212	24	0.35234903437442
2212	25	0.3314084055848241
2213	1	-0.3306353322922746
2213	2	-0.15780004135775982
2213	3	-0.35051854836538965
2213	4	-0.0899845276873279
2213	5	-0.26425838190945683
2213	6	-0.030088873010313932
2213	7	-0.31967945143349397
2213	8	-0.1179995561040812
2213	9	-0.6784048663010225
2213	10	-0.29564039880693826
2213	11	-0.3394326599610906
2213	12	-0.300030358886616
2213	13	-0.49300150567508183
2213	14	-0.42273663980139276
2213	15	-0.40407566958394625
2213	16	-0.40899998029806994
2213	17	-0.3541607612162778
2213	18	-0.456262419797163
2213	19	-0.309639641410989
2213	20	-0.2360179325855256
2213	21	-0.4694128677072271
2213	22	-0.47773905008919115
2213	23	-0.466828944880163
2213	24	-0.11589921478690712
2213	25	0.06890371810066398
2214	1	-2.981179401741347
2214	2	-1.8191549787968242
2214	3	-2.0194119153372334
2214	4	-3.6234607541795967
2214	5	-2.115004846604002
2214	6	-1.761792378079896
2214	7	-1.6041219918111693
2214	8	-1.4627597582877598
2214	9	-1.3330281343495471
2214	10	-1.3103391772070623
2214	11	-1.3866492845796188
2214	12	-1.3990971898671987
2214	13	-1.6186083828821687
2214	14	-1.5597194344424992
2214	15	-2.0692272809004493
2214	16	-1.8638110986375835
2214	17	-3.9199438880112574
2214	18	-3.859696086871086
2214	19	-2.700063419678891
2214	20	-2.282401880597451
2214	21	-2.2613052456221716
2214	22	-3.2198967632344804
2214	23	-2.1397620186962456
2214	24	-3.203706399818117
2214	25	-3.1652902357821375
2215	1	-1.7680601761526091
2215	2	-3.193171245844159
2215	3	-1.901921234447535
2215	4	-3.315076223319533
2215	5	-1.9709173701381353
2215	6	-1.7516042675929064
2215	7	-2.119420989726276
2215	8	-1.3515258787492996
2215	9	-3.717416473082725
2215	10	-2.0568118405022275
2215	11	-1.8452799276026468
2215	12	-1.7169393762522276
2215	13	-1.7671399095197877
2215	14	-1.3670621370145284
2215	15	-1.5167831416639075
2215	16	-1.678627644613215
2215	17	-2.16349623354305
2215	18	-1.6160483147808051
2215	19	-1.829075001989068
2215	20	-1.63224041532618
2215	21	-2.268223052606757
2215	22	-2.2718605887933165
2215	23	-2.081125579393369
2215	24	-1.9566754911558242
2215	25	-2.8823351155994565
2216	1	-3.1243810900138804
2216	2	-3.253282202191457
2216	3	-4.634726959051434
2216	4	-5.009954573896209
2216	5	-4.839756127882268
2216	6	-3.9535319017458583
2216	7	-5.290585663803931
2216	8	-3.1799741086043127
2216	9	-4.029139077973301
2216	10	-2.932889674644507
2216	11	-3.190387602336628
2216	12	-3.60048398516415
2216	13	-3.1477009363943793
2216	14	-4.122577438663182
2216	15	-3.5717898374274313
2216	16	-3.819493944582714
2216	17	-3.5551564294643754
2216	18	-3.08550253848399
2216	19	-3.6810703536553753
2216	20	-4.464494132632424
2216	21	-4.119309466303509
2216	22	-3.7125105708920003
2216	23	-3.803313420565406
2216	24	-4.196772423333343
2216	25	-4.324470217693502
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112563.0
3	1.0
4	1017.0
5	6170.0
6	7818.0
7	7134.0
8	6236.0
9	5727.0
10	5061.0
11	4818.0
12	4412.0
13	4352.0
14	4245.0
15	4349.0
16	4457.0
17	4842.0
18	5455.0
19	6533.0
20	8529.0
21	11128.0
22	13736.0
23	16634.0
24	19485.0
25	22090.0
26	24298.0
27	26905.0
28	30338.0
29	35294.0
30	43305.0
31	53669.0
32	66855.0
33	85077.0
34	109825.0
35	143310.0
36	193463.0
37	295652.0
38	808204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01386454647812	18.1833253777679	12.795099857528577	24.007710218225412
2	15.012634161355901	19.28559560333001	41.98146142245312	23.720308812860967
3	20.086517709845623	24.902419027540578	28.12084333203498	26.890219930578823
4	12.599887231050142	15.655045048562377	37.10602369600687	34.63904402438061
5	13.47808184165283	38.57726616719261	34.055986311450816	13.888665679703749
6	32.78624998807495	35.162991556157436	18.056794120065472	13.993964335702138
7	29.678047433160692	31.265346136146714	20.58223212148166	18.47437430921093
8	26.40543635322664	36.15061297563547	19.86141025291493	17.582540418222962
9	28.05632963320683	14.790935432080706	19.59848009209581	37.554254842616665
10	17.03846297135972	28.114797371771168	32.380171792609445	22.466567864259666
11	35.11406718867483	22.305922225781025	23.760639494074596	18.81937109146955
12	24.92420108419825	24.17250483781781	30.162538864976835	20.74075521300711
13	29.84241770053503	21.573106293433714	25.442224896522305	23.142251109508955
14	21.939199930216173	22.332460884028453	26.602586090574505	29.125753095180873
15	23.931663149573744	30.735772810183743	22.779949617359975	22.552614422882534
16	22.619887557499005	27.53428360497473	27.277369526946448	22.568459310579815
17	22.135779929899048	28.335452234751862	27.039620578819047	22.48914725653005
18	22.0364297615894	26.546279210626402	29.656535878366924	21.760755149417278
19	24.03255537325863	26.28933172249693	27.32339600541045	22.35471689883399
20	24.934233064389353	25.94947463690745	27.690310656197266	21.42598164250593
21	24.13051665416603	25.95527663964681	26.42551565699219	23.48869104919497
22	24.50363986075789	27.466374284284917	26.390660364026967	21.639325490930226
23	23.09416734743207	27.079757232545525	27.582857251900766	22.243218168121643
24	24.034845646633993	25.882533605994674	27.894508957433846	22.188111789937487
25	23.200268960707657	26.770040866580498	27.88163911560994	22.148051057101902
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	285.0
1	285.0
2	660.0
3	1035.0
4	1035.0
5	1035.0
6	1763.0
7	2491.0
8	2491.0
9	2491.0
10	2906.5
11	3322.0
12	3322.0
13	3322.0
14	4502.5
15	5683.0
16	5683.0
17	5683.0
18	11969.0
19	18255.0
20	18255.0
21	18255.0
22	30427.0
23	42599.0
24	42599.0
25	42599.0
26	65378.5
27	88158.0
28	88158.0
29	88158.0
30	117802.5
31	147447.0
32	147447.0
33	147447.0
34	168771.5
35	190096.0
36	190096.0
37	190096.0
38	205822.0
39	221548.0
40	221548.0
41	221548.0
42	241007.0
43	260466.0
44	260466.0
45	260466.0
46	283879.0
47	307292.0
48	307292.0
49	307292.0
50	298855.5
51	290419.0
52	290419.0
53	290419.0
54	259235.0
55	228051.0
56	228051.0
57	228051.0
58	198589.0
59	169127.0
60	169127.0
61	169127.0
62	142348.0
63	115569.0
64	115569.0
65	115569.0
66	88738.5
67	61908.0
68	61908.0
69	61908.0
70	45929.0
71	29950.0
72	29950.0
73	29950.0
74	21252.5
75	12555.0
76	12555.0
77	12555.0
78	8594.5
79	4634.0
80	4634.0
81	4634.0
82	3075.5
83	1517.0
84	1517.0
85	1517.0
86	987.5
87	458.0
88	458.0
89	458.0
90	278.5
91	99.0
92	99.0
93	99.0
94	57.0
95	15.0
96	15.0
97	15.0
98	11.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3385857474419958
2	0.010394977364823307
3	0.009123975765630937
4	0.011484407306988194
5	0.012256086849354988
6	0.07889288497844063
7	0.07716795423667956
8	0.10199787833518764
9	0.08175263857662346
10	0.08901550485772272
11	0.08497553548886126
12	0.09523433411091395
13	0.08116253069128415
14	0.08642810874508111
15	0.08315981891858645
16	0.08452160634629255
17	0.09809408770909678
18	0.10004598302214222
19	0.0935094033691529
20	0.09228379468421738
21	0.080163886577633
22	0.10059069799322465
23	0.09228379468421738
24	0.08924246942900707
25	0.08724518120170478
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2202987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.75355535069863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93976963047184	36.290128655768875
2	13.933762534440264	13.86508451001529
3	5.330359078038046	7.956129462847946
4	2.6356931427703283	5.245404186651214
5	1.446519269591984	3.5984738272748467
6	0.9042359290219364	2.699337140680999
7	0.595079300874359	2.072511765387523
8	0.40988929237899663	1.6314759676829684
9	0.3004765419810076	1.3454798636734726
>10	1.3695593495563791	11.677433356712232
>50	0.0728151790154786	2.506022828037298
>100	0.05218212164591252	4.985033057431743
>500	0.005611204219323474	1.9878343346970677
>1k	0.0038634520854358024	3.548573075817813
>5k	1.8397390883027632E-4	0.5910779673208194
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7426	0.33708778127151906	No Hit
GTCCTACAGTGGACATTTCTAAATT	5489	0.24916170635596127	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4913	0.22301538774400392	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4686	0.21271119620769438	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4368	0.1982762494740096	No Hit
GTACTGGTTCACTATCGGTCAGTCA	4273	0.19396392261960693	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4182	0.18983316742223172	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3726	0.16913399852109887	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2878	0.1306408072312728	No Hit
GGATACCACGTGTCCCGCCCTACTC	2485	0.11280139192832277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	790	0.0	13.225934	4
GGTATCA	2170	0.0	12.990595	1
TTAGGAC	1425	0.0	11.731636	3
GGCGAGG	1105	0.0	11.347032	19
GCCCGTC	60	0.0058843037	11.081477	7
GACGTGA	1330	0.0	10.998158	7
GTCCTAC	2670	0.0	10.985922	1
GTATCAA	4735	0.0	10.861026	1
TGAAACG	70	0.001493631	10.855572	5
GTCCTAA	1320	0.0	10.822171	1
TGTAGGA	2485	0.0	10.7409	2
TCCTACA	2885	0.0	10.667434	2
ACGTGAA	1490	0.0	10.645868	8
AGGACCT	1600	0.0	10.626587	5
TATTAGG	170	1.4006218E-10	10.616111	2
TGGCGAG	2405	0.0	10.585709	18
TAGGACG	2630	0.0	10.582119	4
GGACCTG	1585	0.0	10.54713	6
ATTTCTA	1830	0.0	10.536965	15
AGGACGT	2635	0.0	10.5259905	5
>>END_MODULE