FastQCFastQC Report
Thu 26 May 2016
SRR1512900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202987
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100750.45733361113796855No Hit
TATCAACGCAGAGTACTTTTTTTTT72010.3268743755637233No Hit
GGTATCAACGCAGAGTACTTTTTTT58070.263596653089646No Hit
GTCCTACAGTGGACATTTCTAAATT51510.2338189013371391No Hit
GTCCTAAAGTGTGTATTTCTCATTT44780.20326947004226534No Hit
CTGTAGGACGTGGAATATGGCAAGA44690.20286093381395354No Hit
CTTTAGGACGTGAAATATGGCGAGG39110.17753168765861987No Hit
GTACTGGTTCACTATCGGTCAGTCA39000.1770323656017943No Hit
ACGCAGAGTACTTTTTTTTTTTTTT35230.15991923692695417No Hit
GTCCTACAGTGTGCATTTCTCATTT25900.11756764792529417No Hit
GGATACCACGTGTCCCGCCCTACTC23330.10590166896127849No Hit
CTGAAGGACCTGGAATATGGCGAGA22540.10231562873498573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT400.005235618414.2681534
TAGGACC8050.013.7068814
GGTATCA20950.013.4849371
ACCGCTC500.0014993813.3006048
TAAGCCG550.003040017612.1063125
TGTAGGA27550.011.7040142
CTGTAGG27800.011.4624261
CTAGGAC3250.011.4140043
GTAGGAC26500.011.378113
GACGTGG13700.011.3098577
CTACACT2300.011.1663814
GTCCTAC23450.011.1549181
GTTCTAG1451.193257E-911.152121
CCCGATA600.005909851311.07502911
TATACTG3350.011.0737915
TCGAACT2400.011.07276819
GGCGAGG10200.011.07276719
GTATAAC1553.0559022E-1011.0463131
ATAGGAC2250.010.9912633
AGTTCGC700.001491264710.85763558
Produced by FastQC (version 0.11.5)