##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512900_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2202987
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.22507350247641	31.0	30.0	34.0	26.0	34.0
2	30.216866009649625	31.0	30.0	34.0	26.0	34.0
3	30.391593776994597	31.0	30.0	34.0	26.0	34.0
4	33.99236355003457	37.0	35.0	37.0	30.0	37.0
5	33.58905295401198	37.0	35.0	37.0	28.0	37.0
6	33.46901956298426	37.0	35.0	37.0	28.0	37.0
7	33.30928598307661	37.0	35.0	37.0	27.0	37.0
8	33.25320939252025	37.0	35.0	37.0	27.0	37.0
9	34.56021256593888	39.0	35.0	39.0	27.0	39.0
10	34.484686473410875	39.0	35.0	39.0	27.0	39.0
11	34.478284710713226	39.0	35.0	39.0	27.0	39.0
12	34.33798202168238	39.0	35.0	39.0	26.0	39.0
13	34.33265561712348	39.0	35.0	39.0	26.0	39.0
14	35.40262970230873	40.0	36.0	41.0	26.0	41.0
15	35.40415445029862	40.0	36.0	41.0	26.0	41.0
16	35.408053701633285	40.0	36.0	41.0	26.0	41.0
17	35.408754114300265	40.0	36.0	41.0	26.0	41.0
18	35.32924070818393	40.0	36.0	41.0	25.0	41.0
19	35.3403111321129	40.0	35.0	41.0	25.0	41.0
20	35.31723110485899	40.0	35.0	41.0	25.0	41.0
21	35.28211423853159	40.0	36.0	41.0	25.0	41.0
22	35.250521224137955	40.0	36.0	41.0	25.0	41.0
23	35.09346491831318	40.0	35.0	41.0	25.0	41.0
24	35.05062944084554	40.0	35.0	41.0	24.0	41.0
25	34.98400353701588	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6402692455120906
1101	2	0.5848841962008926
1101	3	0.585501056859659
1101	4	0.5455807488639906
1101	5	0.5730607760938966
1101	6	0.6716257579426426
1101	7	0.664660294241294
1101	8	0.4144474379667855
1101	9	0.8015931259294078
1101	10	0.4460991446217335
1101	11	0.749914992258482
1101	12	0.8146264908377887
1101	13	0.9142877463875223
1101	14	0.9848849478968944
1101	15	0.6691102965565534
1101	16	0.8667783695219313
1101	17	0.9373544187136105
1101	18	0.8921310327480825
1101	19	0.7566586624357043
1101	20	0.744056241695283
1101	21	0.8693523494690183
1101	22	0.8827531281121992
1101	23	0.9737924776228581
1101	24	0.9424967878289578
1101	25	1.039534710084915
1102	1	0.649323802374294
1102	2	0.7427864905153889
1102	3	0.47197798903928145
1102	4	0.5606134632211095
1102	5	0.7908973694695121
1102	6	0.8016920824759879
1102	7	0.7001460373671691
1102	8	0.8013837166141897
1102	9	0.9979687635808432
1102	10	0.8323357276649901
1102	11	1.0117561336120389
1102	12	1.0718493802857978
1102	13	1.07582958848905
1102	14	0.9439970676597298
1102	15	1.0104675599144102
1102	16	1.0886069241821588
1102	17	1.1624712383087754
1102	18	0.40038922381795317
1102	19	0.6864052230339084
1102	20	1.0854275076624944
1102	21	1.0132633408824603
1102	22	0.8113977442350091
1102	23	1.0001796556856917
1102	24	1.0016237190475294
1102	25	1.0831000457818192
1103	1	0.6567180930357921
1103	2	0.846768185831106
1103	3	0.575433062170589
1103	4	0.4928625041656858
1103	5	0.5740516862318188
1103	6	0.6307513232068942
1103	7	0.7048284881989204
1103	8	0.7365453859570437
1103	9	0.7646715534245558
1103	10	0.7489631791471254
1103	11	0.8187955897599082
1103	12	0.9475429175636805
1103	13	0.9328334119162349
1103	14	1.0430815349068467
1103	15	0.9769604344364851
1103	16	1.007750374137487
1103	17	0.8658030576246531
1103	18	0.9179370273198089
1103	19	0.9950185323135585
1103	20	0.9012730011149017
1103	21	0.9941337516503239
1103	22	0.9424772813295021
1103	23	1.0836525984878165
1103	24	1.0039559030070464
1103	25	1.0603111403311445
1104	1	0.7723977354935236
1104	2	0.8765770735348006
1104	3	0.7956867898422857
1104	4	0.7097100711790176
1104	5	0.8068676003356785
1104	6	0.8001363116409266
1104	7	0.7315873518197762
1104	8	0.802842437928426
1104	9	0.8452304406114521
1104	10	0.8910293418313415
1104	11	1.0400618301706999
1104	12	0.9403058287548589
1104	13	0.8166557405301589
1104	14	1.053788123268113
1104	15	0.9750263655947151
1104	16	1.0233509256654543
1104	17	1.0019557986235625
1104	18	0.6194394242338959
1104	19	1.0993283471547883
1104	20	1.0479378486488429
1104	21	0.9804064933934242
1104	22	0.7964332541830856
1104	23	0.891519437972093
1104	24	1.0096490442565056
1104	25	0.8495095436287343
1105	1	0.44930146214986166
1105	2	0.4437926598542923
1105	3	0.4470375545471903
1105	4	0.32890692279187306
1105	5	0.31117693564458904
1105	6	0.4139694004764465
1105	7	0.5905270544437897
1105	8	0.3966536542536261
1105	9	0.6111561171942483
1105	10	0.3278959871350722
1105	11	0.6740912228263625
1105	12	0.7267320839988258
1105	13	0.7528356850453193
1105	14	0.8478888661691073
1105	15	0.8697112573934049
1105	16	0.8406231041655232
1105	17	0.5083502250532419
1105	18	0.5487535858023662
1105	19	0.8066512283288461
1105	20	0.8309849872403987
1105	21	0.6209121384021898
1105	22	0.6787036344727397
1105	23	0.8786784027124668
1105	24	0.83949105652745
1105	25	0.6525435214759625
1106	1	0.045044786090912226
1106	2	0.30020376362164214
1106	3	-1.5398018223835308
1106	4	-0.3260026773232667
1106	5	-0.06058768838779116
1106	6	0.06948571351712474
1106	7	0.2084941807662659
1106	8	0.27136044377710533
1106	9	0.4389446264129404
1106	10	0.3682006190932583
1106	11	0.36428191280116806
1106	12	0.47053876936821126
1106	13	0.4290581655496837
1106	14	0.43809282266038707
1106	15	0.40716154206079125
1106	16	0.46889684730803083
1106	17	0.5173839042709147
1106	18	0.524479953157325
1106	19	0.4749883165296964
1106	20	0.6214855475716732
1106	21	0.5551427861696965
1106	22	0.4503918310927446
1106	23	0.48869414519062815
1106	24	0.5549210383090113
1106	25	0.5840066170283009
1107	1	0.4731344262978645
1107	2	0.5514676126806108
1107	3	0.4376749662925903
1107	4	0.32049914914832556
1107	5	0.4535434860058132
1107	6	0.2741495050065623
1107	7	0.33279008813376265
1107	8	0.39084706875947717
1107	9	0.35711003900416927
1107	10	0.4385758189989062
1107	11	0.49095309925303354
1107	12	0.6303653019919011
1107	13	0.45470984351993593
1107	14	0.681935233916299
1107	15	0.5441595304264624
1107	16	0.7071206212131997
1107	17	0.5479189297194509
1107	18	0.6262467932748734
1107	19	0.6624295820035684
1107	20	0.7456607073930854
1107	21	0.7239951978627417
1107	22	0.597378379783116
1107	23	0.769630466530856
1107	24	0.8403980942133131
1107	25	0.8068902564781055
1108	1	0.5584071424959092
1108	2	0.5065684980731184
1108	3	0.5897985651499695
1108	4	0.5043413215694983
1108	5	0.6652292146155929
1108	6	0.6076351152308277
1108	7	0.5589886634467902
1108	8	0.5389787484438671
1108	9	0.6405501223425816
1108	10	0.7990076450252914
1108	11	0.7291988069969761
1108	12	0.6827542530930586
1108	13	0.8275759757337582
1108	14	0.9206601062770332
1108	15	0.870836567539861
1108	16	0.7320673243013687
1108	17	0.6887605289547878
1108	18	0.9375648708064546
1108	19	0.9212998280361688
1108	20	0.9604767022453515
1108	21	0.9500903810713694
1108	22	0.9709952761145715
1108	23	1.1040891651335016
1108	24	1.127907562712295
1108	25	1.1318475018125795
1109	1	0.4287604412089081
1109	2	0.31002328808292035
1109	3	0.5135782618149314
1109	4	0.46939069837176106
1109	5	0.14833054729755446
1109	6	0.37952285398582575
1109	7	0.3834323218665432
1109	8	0.5393968571838599
1109	9	0.7204057193847291
1109	10	0.357412963063922
1109	11	0.6274480163019547
1109	12	0.8434908921713173
1109	13	0.667681740114574
1109	14	0.8295466617652636
1109	15	0.7844726860441824
1109	16	0.721764972677633
1109	17	0.8502231481934999
1109	18	0.9930707094190225
1109	19	0.839105296441673
1109	20	0.8732429769218513
1109	21	0.9184310159748819
1109	22	0.8267480780458669
1109	23	0.805101286480209
1109	24	0.9167352086458891
1109	25	0.8180059770797641
1110	1	0.3206019599085721
1110	2	0.13514238347445584
1110	3	0.19278792135508027
1110	4	0.2760862631362073
1110	5	0.35065921481977114
1110	6	0.425950389555517
1110	7	0.38560325504200677
1110	8	0.35311974535139967
1110	9	0.18421025593579543
1110	10	0.39555606824531253
1110	11	0.29080795654567027
1110	12	0.4639323894159375
1110	13	0.3377944585885615
1110	14	0.4112804681691955
1110	15	0.4415768900419721
1110	16	0.36454197127510923
1110	17	0.44682331745000425
1110	18	0.4530363813395297
1110	19	0.52512653694086
1110	20	0.5758140070423607
1110	21	0.5588908692064507
1110	22	0.565469674937269
1110	23	0.5728396461224605
1110	24	0.5938057202693585
1110	25	0.5768750575719608
1111	1	0.13976349363473872
1111	2	-0.05741734394503695
1111	3	-0.0742112393357921
1111	4	0.16037605834242186
1111	5	0.33567240705779966
1111	6	0.28931495968233634
1111	7	0.14709357817405078
1111	8	0.22656807703250337
1111	9	0.1939914565929044
1111	10	0.22154731753194312
1111	11	0.19923141419207013
1111	12	0.47743073305059625
1111	13	0.29723609548949526
1111	14	0.09960973886825286
1111	15	0.2627642662970473
1111	16	0.2541716000295011
1111	17	0.3854120414047344
1111	18	0.18064327115765622
1111	19	-0.0077704816802963705
1111	20	0.15245537627811245
1111	21	0.39270726807009027
1111	22	0.10158915222609721
1111	23	0.22476353878384714
1111	24	0.2364323049035022
1111	25	0.23092703802247883
1112	1	0.02807345847991627
1112	2	-0.3541528842921977
1112	3	-0.05440064372752218
1112	4	0.05334713152250714
1112	5	-0.11547470996577403
1112	6	-0.09644838719471949
1112	7	0.030414955609899152
1112	8	-0.04540911682747861
1112	9	-0.017759723201216104
1112	10	-0.34116425641416015
1112	11	-0.21434640957335915
1112	12	0.15234157919373814
1112	13	0.06444851602644519
1112	14	-0.11747843711500394
1112	15	-0.3864556812228983
1112	16	-0.4502439934193134
1112	17	-0.3408803659354831
1112	18	-0.037859705699517576
1112	19	-0.04656125142844303
1112	20	-0.19370684357364354
1112	21	-0.09569590477882173
1112	22	0.031112303509551964
1112	23	0.18659672604199784
1112	24	0.04648342610231282
1112	25	-0.05777865562061635
1113	1	-0.572923206583166
1113	2	-0.4563402985983096
1113	3	-0.41073418001679585
1113	4	-2.0749108296440397
1113	5	-0.7021667328131187
1113	6	-0.43032706880993743
1113	7	-0.4231091159675273
1113	8	-0.22763501719469303
1113	9	-0.21310915741884173
1113	10	-0.2954426964184975
1113	11	-0.4141714699982657
1113	12	-0.12300834231177049
1113	13	-0.2518547245105509
1113	14	-0.34045225337859364
1113	15	-0.49660057110995126
1113	16	-0.5717276849670583
1113	17	-0.6815290412990578
1113	18	-0.5175403256071505
1113	19	-0.1966887570784408
1113	20	-0.048816553131516116
1113	21	-0.1551706003792006
1113	22	-0.17775130162849706
1113	23	-0.08436663644443598
1113	24	0.22929237770970445
1113	25	-0.11163074293517639
1114	1	-0.8224819122423774
1114	2	-1.9936220469142256
1114	3	-1.2997449771209908
1114	4	-0.821596296414036
1114	5	-0.6621860337744323
1114	6	-0.30208941019490965
1114	7	-0.2910996671024577
1114	8	-0.4035856446277535
1114	9	-0.7374234844353182
1114	10	-0.7387245642810001
1114	11	-0.6145551468747712
1114	12	-0.7033898451407694
1114	13	-0.7206093848104231
1114	14	-0.754690373349753
1114	15	-0.908900006334747
1114	16	-1.031745120723997
1114	17	-1.0627161736098003
1114	18	-0.811199749873218
1114	19	-1.294622310170439
1114	20	-0.6648232859120142
1114	21	-1.006326944672331
1114	22	-0.6222465933755217
1114	23	-0.5182087483693607
1114	24	-0.6254814220161009
1114	25	-1.241785744888439
1115	1	-1.0622959295829837
1115	2	-1.2227508131748976
1115	3	-0.7373051239757267
1115	4	-0.45296820908093594
1115	5	-0.8153714112012835
1115	6	-0.6538204685911353
1115	7	-0.6794619980067012
1115	8	-0.8341990883973907
1115	9	-1.8803125127509404
1115	10	-1.5436796173563891
1115	11	-2.0012640500972623
1115	12	-2.602500468512673
1115	13	-2.455619577968882
1115	14	-2.0675991188279
1115	15	-2.403506445818799
1115	16	-1.9171011874626345
1115	17	-1.9323434141104059
1115	18	-2.3568581793079773
1115	19	-2.795769029491339
1115	20	-2.9644367259836883
1115	21	-2.201739750420863
1115	22	-2.210511579924635
1115	23	-2.0810440189340227
1115	24	-2.760209672253076
1115	25	-2.7590899014466146
1116	1	-1.2428235696810503
1116	2	-1.3554433982052956
1116	3	-1.3550404424270006
1116	4	-1.009007815295874
1116	5	-1.3719455646924388
1116	6	-2.104163360649398
1116	7	-1.5538841859882098
1116	8	-1.8251666246600742
1116	9	-2.9264257069792734
1116	10	-1.6720497130701446
1116	11	-2.549408979393565
1116	12	-4.643877122250519
1116	13	-3.4158370781776135
1116	14	-2.8891125119222423
1116	15	-3.9594458599990077
1116	16	-3.6749882740756163
1116	17	-3.2444505209135954
1116	18	-3.140231045483297
1116	19	-3.2710948433618654
1116	20	-5.191177942102463
1116	21	-4.132846530840908
1116	22	-3.4811287476779853
1116	23	-4.065717483984457
1116	24	-3.964251510017885
1116	25	-3.277640798241233
1201	1	0.5729743032756076
1201	2	0.8480142057429418
1201	3	0.8751868013372643
1201	4	0.7016812726010642
1201	5	0.10992619869136178
1201	6	0.7089662468825324
1201	7	0.9097440680057929
1201	8	0.9141218893831251
1201	9	0.7536298826882728
1201	10	0.8458740447064272
1201	11	0.5590935944524489
1201	12	1.1165978324706458
1201	13	1.1373392949586147
1201	14	1.1084546919116889
1201	15	1.0095330201752333
1201	16	1.0651191207597037
1201	17	0.9497610287618272
1201	18	1.1107559173011623
1201	19	1.2257993566991487
1201	20	1.2429878846369604
1201	21	0.9750728245583744
1201	22	1.128548372703861
1201	23	1.1423607817878292
1201	24	0.730249380111907
1201	25	0.906638563491299
1202	1	0.646750024663536
1202	2	0.7028078924887424
1202	3	0.7016210258756885
1202	4	0.5139017179189977
1202	5	0.6117781543366192
1202	6	0.6214257449015435
1202	7	0.7188430867390636
1202	8	0.5220548561491896
1202	9	0.9113172752293579
1202	10	0.8701170895928456
1202	11	0.7835012401578751
1202	12	0.855575714988575
1202	13	0.9426752110598642
1202	14	0.9954954373803702
1202	15	0.9577594537711889
1202	16	1.0668618605226143
1202	17	0.8765794033606014
1202	18	0.8927232139976766
1202	19	0.9667913162800232
1202	20	0.9483746808471025
1202	21	1.0609468894792258
1202	22	0.8940304442751028
1202	23	1.060042824409436
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2211	8	-0.24848104608770427
2211	9	0.05907982522437294
2211	10	-0.01877044516823645
2211	11	0.12618618016385597
2211	12	-0.10732216777103787
2211	13	0.14074077792854212
2211	14	0.07375245330121771
2211	15	0.007744496813991475
2211	16	0.0156691816761807
2211	17	-0.1690160497244122
2211	18	-0.10044865805940617
2211	19	-0.12723587214440357
2211	20	-0.12386742243345594
2211	21	-0.09608565021210325
2211	22	0.06406002581267956
2211	23	-0.15487313964954552
2211	24	7.13713437903607E-4
2211	25	-0.20589037884930406
2212	1	-0.18159873154980133
2212	2	-0.03633613343078679
2212	3	-0.023543184629918557
2212	4	-0.11455036633235949
2212	5	-0.31614511153497205
2212	6	-0.18045868577488022
2212	7	-0.31177505306451536
2212	8	-0.09243199937778712
2212	9	-0.1711061709404973
2212	10	-0.3908716912075434
2212	11	-0.1789868827149732
2212	12	0.09102388084805568
2212	13	0.15357160383938862
2212	14	0.13047998397008342
2212	15	-0.12062924622572524
2212	16	-0.1786394178480748
2212	17	-0.21801002033964778
2212	18	-0.20367665533272117
2212	19	0.06162515183646633
2212	20	-0.1901990394337858
2212	21	0.040092502973344324
2212	22	0.08839791283654819
2212	23	-0.2924404577178308
2212	24	-0.44645232480102237
2212	25	-0.25485894404690157
2213	1	-0.40169612986939995
2213	2	0.033077145578406686
2213	3	-0.06413325020401928
2213	4	-0.19974602445513767
2213	5	-0.06025915169023932
2213	6	-0.05833477403133713
2213	7	-0.3858352758340331
2213	8	-0.37109963901414034
2213	9	-0.11239251794608407
2213	10	-0.32122378461263423
2213	11	-0.09457872193173245
2213	12	-0.3918569202303175
2213	13	-0.24518127199549156
2213	14	-0.21654283014203202
2213	15	-0.5032333590496663
2213	16	-1.0425625127024887
2213	17	-0.547870054596288
2213	18	-0.22443587574075963
2213	19	-0.2869868460959708
2213	20	-0.15548074456316385
2213	21	-0.11838044137481774
2213	22	-0.19705872413427272
2213	23	-0.3445110588306619
2213	24	-0.1226399052483842
2213	25	-0.08149702972904294
2214	1	-1.2383001386351609
2214	2	-0.5412886503303191
2214	3	-0.7021800303037011
2214	4	-0.8091505275730455
2214	5	-1.1509438576265225
2214	6	-1.6037883738634982
2214	7	-1.2525165756064283
2214	8	-1.0367899425541225
2214	9	-0.8786438159730849
2214	10	-1.2164427258457309
2214	11	-1.3397172692345691
2214	12	-0.9975715887728427
2214	13	-1.2927622256861184
2214	14	-0.8875699860128137
2214	15	-1.0515219027383012
2214	16	-1.2157387957878498
2214	17	-1.2666238781562953
2214	18	-1.1744871797620462
2214	19	-1.360031607532271
2214	20	-1.374431762445525
2214	21	-1.4993990994661317
2214	22	-1.8808582012211232
2214	23	-1.9064908122540203
2214	24	-1.629473565622817
2214	25	-2.1069041562278343
2215	1	-1.3060659837734079
2215	2	-0.7650550058239389
2215	3	-0.9312561669135349
2215	4	-0.9039941888285554
2215	5	-1.374744319279003
2215	6	-1.3095047074620183
2215	7	-1.7506762567544882
2215	8	-1.4757581199174545
2215	9	-1.4332339924804103
2215	10	-1.5214068330896353
2215	11	-1.141557393699074
2215	12	-1.447458066511416
2215	13	-1.5627429565899433
2215	14	-1.8568539439954392
2215	15	-1.5111647741446532
2215	16	-1.6352690249777027
2215	17	-1.703007409511521
2215	18	-1.4150582405625443
2215	19	-1.7198090834008397
2215	20	-1.6185553419593504
2215	21	-1.3651500128219993
2215	22	-1.6073334339647332
2215	23	-1.8202838730467406
2215	24	-1.8066800662213538
2215	25	-2.0469450871527854
2216	1	-1.6588608862987115
2216	2	-1.0070709837641445
2216	3	-0.9849656799871518
2216	4	-1.40426659630986
2216	5	-2.048140119308762
2216	6	-2.2359861429319317
2216	7	-2.1614704495889434
2216	8	-2.3518336567536977
2216	9	-4.34804387626853
2216	10	-2.271914537723937
2216	11	-2.4476461206062865
2216	12	-2.657806741018849
2216	13	-2.66146888833309
2216	14	-2.8384291961424495
2216	15	-2.738890006446468
2216	16	-2.1394650544553215
2216	17	-2.3790748881050447
2216	18	-2.863664146901023
2216	19	-2.890330069998356
2216	20	-2.207413372333974
2216	21	-3.098132895064566
2216	22	-2.3957813245253163
2216	23	-3.6035061875931795
2216	24	-2.8311618381582377
2216	25	-3.0794697275272007
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62335.0
3	2.0
4	1016.0
5	8596.0
6	10633.0
7	8342.0
8	6760.0
9	5551.0
10	4734.0
11	4285.0
12	3843.0
13	3513.0
14	3507.0
15	3578.0
16	3578.0
17	3890.0
18	4253.0
19	5127.0
20	6896.0
21	9117.0
22	11527.0
23	13821.0
24	16460.0
25	18861.0
26	20961.0
27	23128.0
28	26306.0
29	31414.0
30	39238.0
31	49777.0
32	63753.0
33	81727.0
34	106350.0
35	138910.0
36	190627.0
37	295140.0
38	915431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89951947910936	19.1882617882537	13.85251275127172	26.059705981365223
2	13.611256056483736	19.931979369467193	40.56160390267119	25.89516067137788
3	18.11199866337474	24.849425351023037	28.9681743351606	28.070401650441624
4	11.930640729346406	16.323315851426948	36.377936027389985	35.36810739183666
5	13.11594123939191	38.72845465557414	34.129768760408325	14.025835344625628
6	32.841108013411855	35.82175783985894	17.49427924895782	13.842854897771383
7	29.15016009856693	31.45800547351741	20.664390880378566	18.7274435475371
8	26.373289463165772	36.48966592298133	19.493261411451783	17.64378320240111
9	27.416168375665933	14.640651555308146	19.56995242832578	38.373227640700144
10	16.47808094001893	28.706102384019093	32.3105165344432	22.505300141518774
11	35.022886250590716	22.19096979323613	23.716810624050378	19.06933333212278
12	24.457188820940402	24.51801562976628	30.23214960126665	20.79264594802667
13	29.6537582608743	21.56225514718633	25.53228675164128	23.251699840298087
14	21.63641257893382	22.180046510297768	26.830486170154334	29.353054740614077
15	23.82909098007917	30.72104796499156	22.801564828850818	22.648296226078454
16	22.57285443005507	27.55895610234871	27.146704872881262	22.72148459471496
17	21.862256652132565	28.36674058555563	27.15468265191013	22.61632011040168
18	21.834395355519813	26.408558278212134	29.953882951945026	21.803163414323024
19	23.833463765563348	26.186600403462297	27.649242010193685	22.33069382078067
20	24.67899686691137	25.914891839897447	27.97536744111727	21.430743852073913
21	23.687422689771786	25.714045779003392	27.04224968508494	23.55628184613988
22	24.10581915472141	27.280266694992118	26.885798509827207	21.728115640459272
23	22.7026305647741	26.88704018680092	28.006837988603657	22.403491259821323
24	23.801320661447388	25.837556009182077	28.2330762732599	22.128047056110635
25	22.881419020746403	26.621981821020942	28.257445355935406	22.23915380229725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	98.0
2	339.5
3	581.0
4	581.0
5	581.0
6	1382.0
7	2183.0
8	2183.0
9	2183.0
10	2729.5
11	3276.0
12	3276.0
13	3276.0
14	4499.0
15	5722.0
16	5722.0
17	5722.0
18	12165.5
19	18609.0
20	18609.0
21	18609.0
22	31702.0
23	44795.0
24	44795.0
25	44795.0
26	69396.5
27	93998.0
28	93998.0
29	93998.0
30	122226.0
31	150454.0
32	150454.0
33	150454.0
34	170627.5
35	190801.0
36	190801.0
37	190801.0
38	206865.0
39	222929.0
40	222929.0
41	222929.0
42	242437.5
43	261946.0
44	261946.0
45	261946.0
46	284375.5
47	306805.0
48	306805.0
49	306805.0
50	297447.0
51	288089.0
52	288089.0
53	288089.0
54	257172.5
55	226256.0
56	226256.0
57	226256.0
58	196301.0
59	166346.0
60	166346.0
61	166346.0
62	139672.0
63	112998.0
64	112998.0
65	112998.0
66	86411.0
67	59824.0
68	59824.0
69	59824.0
70	44513.5
71	29203.0
72	29203.0
73	29203.0
74	20536.0
75	11869.0
76	11869.0
77	11869.0
78	8144.5
79	4420.0
80	4420.0
81	4420.0
82	2880.5
83	1341.0
84	1341.0
85	1341.0
86	853.0
87	365.0
88	365.0
89	365.0
90	218.5
91	72.0
92	72.0
93	72.0
94	39.5
95	7.0
96	7.0
97	7.0
98	3.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08325060474710018
2	0.045756057570925296
3	0.01929198855916989
4	0.006264222167448106
5	0.01920120273065615
6	0.06141661298954556
7	0.08365914097541202
8	0.060781112189949374
9	0.028415964324800827
10	0.052610387623712715
11	0.006173436338934366
12	4.993220568255736E-4
13	0.006037257596163754
14	0.0015433590847335915
15	0.014434946733684766
16	0.009260154508401548
17	0.00553793553933818
18	0.0051747922252832175
19	0.002496610284127868
20	0.002224252798586646
21	5.44714971082444E-4
22	9.532511993942769E-4
23	0.0
24	0.0
25	1.36178742770611E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2202987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.93130022900419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.17285857345202	34.11407652440583
2	14.82394375426761	14.210617973273445
3	5.688123895710755	8.179175225552553
4	2.7973834166052014	5.363288975877656
5	1.5259458179167855	3.6570283565881407
6	0.9513306338200342	2.7359108536006143
7	0.6627053996120356	2.223503203053067
8	0.43223293901552984	1.6573989415054575
9	0.31987967254012917	1.3799023759508828
>10	1.480238890774162	12.159393772790809
>50	0.08058723583130128	2.6700823575138775
>100	0.05459668723406544	5.07537470332389
>500	0.0056094571028396	1.8865003892414431
>1k	0.004183323941100689	3.401096300462736
>5k	2.852266323477742E-4	0.8275227810767734
>10k+	9.507554411592474E-5	0.45912726578272434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10075	0.45733361113796855	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7201	0.3268743755637233	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5807	0.263596653089646	No Hit
GTCCTACAGTGGACATTTCTAAATT	5151	0.2338189013371391	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4478	0.20326947004226534	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4469	0.20286093381395354	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3911	0.17753168765861987	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3900	0.1770323656017943	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3523	0.15991923692695417	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2590	0.11756764792529417	No Hit
GGATACCACGTGTCCCGCCCTACTC	2333	0.10590166896127849	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2254	0.10231562873498573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.539291425687033E-5
2	0.0	0.0	0.0	0.0	4.539291425687033E-5
3	0.0	0.0	0.0	0.0	4.539291425687033E-5
4	0.0	0.0	0.0	0.0	4.539291425687033E-5
5	0.0	0.0	0.0	0.0	4.539291425687033E-5
6	0.0	0.0	0.0	0.0	1.36178742770611E-4
7	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
8	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
9	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
10	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
11	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
12	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
13	0.0	0.0	0.0	4.539291425687033E-5	1.8157165702748133E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAACGT	40	0.0052356184	14.268153	4
TAGGACC	805	0.0	13.706881	4
GGTATCA	2095	0.0	13.484937	1
ACCGCTC	50	0.00149938	13.300604	8
TAAGCCG	55	0.0030400176	12.106312	5
TGTAGGA	2755	0.0	11.704014	2
CTGTAGG	2780	0.0	11.462426	1
CTAGGAC	325	0.0	11.414004	3
GTAGGAC	2650	0.0	11.37811	3
GACGTGG	1370	0.0	11.309857	7
CTACACT	230	0.0	11.166381	4
GTCCTAC	2345	0.0	11.154918	1
GTTCTAG	145	1.193257E-9	11.15212	1
CCCGATA	60	0.0059098513	11.075029	11
TATACTG	335	0.0	11.073791	5
TCGAACT	240	0.0	11.072768	19
GGCGAGG	1020	0.0	11.072767	19
GTATAAC	155	3.0559022E-10	11.046313	1
ATAGGAC	225	0.0	10.991263	3
AGTTCGC	70	0.0014912647	10.8576355	8
>>END_MODULE