FastQCFastQC Report
Thu 26 May 2016
SRR1512899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979711
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66420.33550351541209805No Hit
TATCAACGCAGAGTACTTTTTTTTT39160.19780664955642516No Hit
GGTATCAACGCAGAGTACTTTTTTT38730.1956346153554736No Hit
GTACTGGTTCACTATCGGTCAGTCA28670.14481911753786283No Hit
GTCCTACAGTGGACATTTCTAAATT21170.10693480007940553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCC759.688611E-713.9317948
GGTATCA16700.013.8589781
TAGGACC4100.013.6688754
CCCCGTA2300.011.15009615
CCCGTAC2150.011.04501216
CCCTAAT951.3223302E-511.028351
AGGCCGT951.3622692E-510.9987856
GTATCAA37600.010.867031
GGGCCGT700.001493088910.8559446
CCGTACA2200.010.79426217
TCTAGGA3200.010.6860492
CAAGACG803.7763864E-410.6860494
GCGCCAC1605.456968E-1010.68550913
TCCTATA1909.094947E-1210.4985742
GTCTTAG2100.010.4315771
GTCCTAA6200.010.1389661
TGTAGGA13150.010.0404622
GGCGTGC1803.947207E-1010.02666958
GTCTAAC951.6021729E-410.0257721
AGGACCT9150.09.9656095
Produced by FastQC (version 0.11.5)