##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512899_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1979711 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.76553042337998 31.0 30.0 34.0 22.0 34.0 2 28.959112718977668 31.0 30.0 34.0 20.0 34.0 3 29.050001742678603 31.0 30.0 34.0 22.0 34.0 4 32.49837779352643 35.0 33.0 37.0 24.0 37.0 5 32.67630679427452 35.0 33.0 37.0 25.0 37.0 6 32.56357013725741 36.0 33.0 37.0 25.0 37.0 7 32.49753474118192 37.0 33.0 37.0 23.0 37.0 8 32.39648867940826 36.0 33.0 37.0 22.0 37.0 9 33.691826231202434 38.0 34.0 39.0 21.0 39.0 10 33.575333470390376 38.0 34.0 39.0 19.0 39.0 11 33.6272248828238 38.0 34.0 39.0 20.0 39.0 12 33.52615305971427 38.0 34.0 39.0 18.0 39.0 13 33.4906231263048 38.0 34.0 39.0 18.0 39.0 14 34.33937630290482 39.0 34.0 41.0 17.0 41.0 15 34.49374176331798 39.0 34.0 41.0 17.0 41.0 16 34.43966770907471 39.0 34.0 41.0 17.0 41.0 17 34.19142794074489 39.0 33.0 41.0 17.0 41.0 18 34.19259225210144 39.0 33.0 41.0 17.0 41.0 19 34.31360233892725 39.0 34.0 41.0 18.0 41.0 20 34.274057678115646 39.0 34.0 41.0 17.0 41.0 21 34.17773250742154 39.0 34.0 41.0 13.0 41.0 22 34.17873113802974 39.0 34.0 41.0 10.0 41.0 23 33.839058327200284 39.0 34.0 41.0 8.0 41.0 24 33.899657071158366 39.0 34.0 41.0 2.0 41.0 25 33.611958513136514 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8143641295370188 1101 2 0.6068043612803002 1101 3 0.8443217449334881 1101 4 0.9472902120431925 1101 5 0.801164461747959 1101 6 0.8240842687933849 1101 7 0.5222305358720263 1101 8 0.953074775454354 1101 9 0.9536858568250324 1101 10 1.0401616175431627 1101 11 0.9557455936517272 1101 12 0.9677926650274173 1101 13 1.3408838584453164 1101 14 1.3902904939979237 1101 15 1.3618587171336856 1101 16 1.2414802899995365 1101 17 0.46998645589786037 1101 18 1.0758996236210834 1101 19 0.4976842019673384 1101 20 -0.07452902755536428 1101 21 0.9382799491995968 1101 22 1.079886528837541 1101 23 0.4847984874882698 1101 24 1.067756479887933 1101 25 0.7054417904221566 1102 1 0.9055628117525281 1102 2 0.9180822359832739 1102 3 0.8900202446210628 1102 4 1.0346102316621426 1102 5 0.9122122168783378 1102 6 0.7669858358460857 1102 7 0.698854194627657 1102 8 0.8671622365691789 1102 9 1.1576273403852042 1102 10 1.108137819484007 1102 11 0.9239338863324633 1102 12 1.0878438624330613 1102 13 0.9395925013109405 1102 14 1.1095391283978273 1102 15 1.0483077537669345 1102 16 1.1692473798771843 1102 17 1.3101679088202118 1102 18 1.271981991870632 1102 19 1.0975136559288643 1102 20 1.3114807102538961 1102 21 1.4634216603037942 1102 22 1.2522058877725613 1102 23 1.088495406013621 1102 24 0.5930217052931326 1102 25 0.9055249918053434 1103 1 0.5381909373531855 1103 2 0.6458557424223166 1103 3 0.7036042667077673 1103 4 0.7880940423175815 1103 5 0.6025230732623328 1103 6 0.3478006325400713 1103 7 0.2228538276808294 1103 8 0.44887517930800414 1103 9 0.5461218099722984 1103 10 0.6618352456141139 1103 11 0.4682348526257769 1103 12 0.5788597809359146 1103 13 0.7951102458111023 1103 14 0.8413407499533321 1103 15 0.6747890831628709 1103 16 0.6622054955178598 1103 17 0.3378671522107979 1103 18 0.6210720511063315 1103 19 0.5883382378715893 1103 20 0.7953311182638174 1103 21 0.6080594313639196 1103 22 0.6601747497131853 1103 23 0.048910143297170805 1103 24 0.18508473277919535 1103 25 0.655534165476034 1104 1 0.9091362421568796 1104 2 0.8774836290172594 1104 3 0.9249220278991572 1104 4 1.0794703254476374 1104 5 0.6932592555706663 1104 6 0.6543323028149359 1104 7 0.8232054270164397 1104 8 0.5317969194350312 1104 9 0.9588780251631448 1104 10 0.9287216627433565 1104 11 0.6352768760042196 1104 12 0.93103531894252 1104 13 1.0277754020620264 1104 14 1.2386059690703632 1104 15 0.9661599940219361 1104 16 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2214 7 -1.2177696382320207 2214 8 -1.0866520222333271 2214 9 -0.9333402289203292 2214 10 -0.98910560385427 2214 11 -0.8457873594515846 2214 12 -0.8641151638476927 2214 13 -1.3196786981867064 2214 14 -1.1971214746417544 2214 15 -1.5761877891434821 2214 16 -1.3846843751868008 2214 17 -3.639511107361688 2214 18 -3.2681610536238352 2214 19 -2.124278825605124 2214 20 -1.988761295360895 2214 21 -2.0082155621665834 2214 22 -2.9917085827174255 2214 23 -1.889350344807987 2214 24 -2.9120119683369943 2214 25 -2.8560867461682875 2215 1 -1.784628831912002 2215 2 -3.4951969067629207 2215 3 -2.024448537192729 2215 4 -3.6368373811882684 2215 5 -2.138658365924112 2215 6 -1.9603223368221272 2215 7 -1.9468264762301217 2215 8 -1.368502271569902 2215 9 -3.893546555774577 2215 10 -2.1879416717052145 2215 11 -1.7965555081953006 2215 12 -1.5674483784124398 2215 13 -1.6658083655985187 2215 14 -1.4515533244521635 2215 15 -1.6592110590079088 2215 16 -1.7412751797097172 2215 17 -2.0726145976606176 2215 18 -1.7953834849820467 2215 19 -1.8791023535588565 2215 20 -1.6677150370619387 2215 21 -2.1907481112141376 2215 22 -2.0446822641150106 2215 23 -1.9844058019921675 2215 24 -1.9635488596673518 2215 25 -2.7335200434062976 2216 1 -3.3160338772092857 2216 2 -3.3210254960083887 2216 3 -4.589448413422467 2216 4 -5.124944217572931 2216 5 -4.935778468900974 2216 6 -3.945330966506237 2216 7 -5.515487108174753 2216 8 -3.4568737129773233 2216 9 -4.17928047920239 2216 10 -3.018313840987119 2216 11 -3.515041571400296 2216 12 -3.9029715500491413 2216 13 -3.529783476715245 2216 14 -4.558208951864692 2216 15 -3.831065385094629 2216 16 -4.1399123374388545 2216 17 -3.8395447633178996 2216 18 -3.273682449683296 2216 19 -4.106734196540216 2216 20 -4.563100512963867 2216 21 -4.3608266763639065 2216 22 -3.943002306628781 2216 23 -3.993557111929235 2216 24 -4.340718354879549 2216 25 -4.276769955582079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100794.0 3 1.0 4 904.0 5 5578.0 6 6846.0 7 6491.0 8 5676.0 9 5103.0 10 4397.0 11 4401.0 12 4182.0 13 4015.0 14 3911.0 15 3962.0 16 4223.0 17 4465.0 18 5086.0 19 6131.0 20 7698.0 21 10107.0 22 12382.0 23 15174.0 24 17508.0 25 19913.0 26 21917.0 27 24113.0 28 27112.0 29 32280.0 30 38678.0 31 47991.0 32 59508.0 33 75745.0 34 97960.0 35 128126.0 36 173504.0 37 265431.0 38 728398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.130752809376006 18.051520600739167 12.488368179800064 24.32935841008476 2 15.717855169048251 19.74918842896215 40.32939731062585 24.20355909136375 3 19.612663089026263 25.08313896773809 28.36095875913165 26.943239184104 4 12.765915527395553 15.863897726779024 37.197711320436355 34.17247542538907 5 13.92693660237469 38.070654658671884 33.76599363765554 14.236415101297881 6 32.553087692957114 35.24780154643736 18.09508643417025 14.104024326435283 7 29.293628353747465 31.529535827650758 20.858864691427176 18.317971127174598 8 26.838201273362294 34.619202376214794 20.107564779310668 18.435031571112244 9 28.106482401314043 14.576265016933625 19.32496030115009 37.99229228060224 10 17.243256962463064 27.741555150635122 32.05796878697118 22.957219099930633 11 35.20435273008736 22.341260793224592 23.324639265269514 19.129747211418536 12 24.507278260680504 24.342797507658997 29.77956140825458 21.37036282340592 13 29.593670123273046 21.31106019671919 25.186496990972916 23.908772689034848 14 22.603950689937424 21.435508685076794 26.60984977752027 29.35069084746551 15 24.528990599529017 28.95364274570194 22.94389343503948 23.573473219729564 16 23.48940933321469 27.20701244913972 25.916091665790347 23.38748655185524 17 22.685727807874375 27.462373072640727 26.357456568367592 23.494442551117302 18 22.58399059090987 26.366436731858123 28.184037515005087 22.865535162226912 19 24.293998411360544 26.055456764181084 26.905646849796717 22.744897974661658 20 24.95123413782259 25.629810225207777 27.01871456322068 22.40024107374895 21 25.01333368384281 25.430911801536144 26.186192319494783 23.369562195126267 22 24.97216410084231 26.139653793216432 26.233046900835433 22.65513520510583 23 24.00234605291765 26.10272071352368 26.658189190965675 23.236744042592996 24 24.119416024737447 25.649782697943063 26.984638190558435 23.246163086761058 25 23.83054883551619 26.068764330115297 26.928204700404212 23.1724821339643 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 634.0 3 1021.0 4 1021.0 5 1021.0 6 1735.0 7 2449.0 8 2449.0 9 2449.0 10 2775.0 11 3101.0 12 3101.0 13 3101.0 14 4399.0 15 5697.0 16 5697.0 17 5697.0 18 11302.5 19 16908.0 20 16908.0 21 16908.0 22 26778.0 23 36648.0 24 36648.0 25 36648.0 26 56221.0 27 75794.0 28 75794.0 29 75794.0 30 96902.5 31 118011.0 32 118011.0 33 118011.0 34 137063.0 35 156115.0 36 156115.0 37 156115.0 38 173213.5 39 190312.0 40 190312.0 41 190312.0 42 205940.5 43 221569.0 44 221569.0 45 221569.0 46 241895.5 47 262222.0 48 262222.0 49 262222.0 50 262662.5 51 263103.0 52 263103.0 53 263103.0 54 241413.0 55 219723.0 56 219723.0 57 219723.0 58 194034.5 59 168346.0 60 168346.0 61 168346.0 62 142619.5 63 116893.0 64 116893.0 65 116893.0 66 91487.0 67 66081.0 68 66081.0 69 66081.0 70 49636.0 71 33191.0 72 33191.0 73 33191.0 74 23643.5 75 14096.0 76 14096.0 77 14096.0 78 9830.0 79 5564.0 80 5564.0 81 5564.0 82 3736.0 83 1908.0 84 1908.0 85 1908.0 86 1224.5 87 541.0 88 541.0 89 541.0 90 337.0 91 133.0 92 133.0 93 133.0 94 80.0 95 27.0 96 27.0 97 27.0 98 19.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33717042538027014 2 0.010961195851313651 3 0.00979941011592096 4 0.011567344930648968 5 0.01272913066604166 6 0.08162807601715605 7 0.08021372816537363 8 0.1066822379630158 9 0.0855680450328356 10 0.09188209794257848 11 0.08758854196395333 12 0.09961049870410377 13 0.08400215991121936 14 0.08981108858818282 15 0.08713393015445184 16 0.08804315377345481 17 0.10127740867227591 18 0.1034999552965054 19 0.09688282784709486 20 0.09562001726514627 21 0.08273934932927078 22 0.1034999552965054 23 0.09703436511692869 24 0.09188209794257848 25 0.0907203122071858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1979711.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.145151941674214 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2843785425265 36.24711145024486 2 14.27554282556279 14.316985280754466 3 5.473476871574335 8.23404988123004 4 2.5744505271186413 5.163848513947502 5 1.4922196961151257 3.7413791696025953 6 0.9148795787162871 2.7526065289837867 7 0.6059033840346411 2.1268182078074163 8 0.42011935314150584 1.6853559037534955 9 0.31516399744342793 1.4223551884511505 >10 1.5010266828326606 13.126714551119639 >50 0.08648632935457734 2.9588894484881116 >100 0.050261588430775286 4.67898228231339 >500 0.003958905046688767 1.41575226720685 >1k 0.0020302077162506482 1.791056673999216 >5k 1.0151038581253243E-4 0.3380946520975045 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6642 0.33550351541209805 No Hit TATCAACGCAGAGTACTTTTTTTTT 3916 0.19780664955642516 No Hit GGTATCAACGCAGAGTACTTTTTTT 3873 0.1956346153554736 No Hit GTACTGGTTCACTATCGGTCAGTCA 2867 0.14481911753786283 No Hit GTCCTACAGTGGACATTTCTAAATT 2117 0.10693480007940553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.051242327794309E-5 7 0.0 0.0 0.0 0.0 5.051242327794309E-5 8 0.0 0.0 0.0 5.051242327794309E-5 5.051242327794309E-5 9 0.0 0.0 0.0 1.0102484655588618E-4 5.051242327794309E-5 10 0.0 0.0 0.0 1.0102484655588618E-4 5.051242327794309E-5 11 0.0 0.0 0.0 1.0102484655588618E-4 5.051242327794309E-5 12 0.0 0.0 0.0 1.0102484655588618E-4 5.051242327794309E-5 13 0.0 0.0 0.0 1.0102484655588618E-4 5.051242327794309E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCC 75 9.688611E-7 13.931794 8 GGTATCA 1670 0.0 13.858978 1 TAGGACC 410 0.0 13.668875 4 CCCCGTA 230 0.0 11.150096 15 CCCGTAC 215 0.0 11.045012 16 CCCTAAT 95 1.3223302E-5 11.02835 1 AGGCCGT 95 1.3622692E-5 10.998785 6 GTATCAA 3760 0.0 10.86703 1 GGGCCGT 70 0.0014930889 10.855944 6 CCGTACA 220 0.0 10.794262 17 TCTAGGA 320 0.0 10.686049 2 CAAGACG 80 3.7763864E-4 10.686049 4 GCGCCAC 160 5.456968E-10 10.685509 13 TCCTATA 190 9.094947E-12 10.498574 2 GTCTTAG 210 0.0 10.431577 1 GTCCTAA 620 0.0 10.138966 1 TGTAGGA 1315 0.0 10.040462 2 GGCGTGC 180 3.947207E-10 10.0266695 8 GTCTAAC 95 1.6021729E-4 10.025772 1 AGGACCT 915 0.0 9.965609 5 >>END_MODULE