FastQCFastQC Report
Thu 26 May 2016
SRR1512899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979711
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84200.42531460400028087No Hit
TATCAACGCAGAGTACTTTTTTTTT60580.30600426021777927No Hit
GGTATCAACGCAGAGTACTTTTTTT49570.2503900821887639No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28550.14421296845852752No Hit
GTACTGGTTCACTATCGGTCAGTCA24420.12335133764473703No Hit
GTCCTACAGTGGACATTTCTAAATT20160.10183304532833327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16850.014.0562861
TAGGACC5600.012.3967274
GTGTCGT550.00308418512.08291911
TCCAACG907.5311436E-611.6016818
GTATCAA40850.011.2700131
TATACAC3850.011.1176223
TGGCGAG11100.010.518476518
AGGACCT9300.010.4301225
GTGACAC3100.010.41089219
GTAGGAC12150.010.3337473
GGACCGT1303.7893915E-710.2410846
TGTAGGA12950.010.1360662
GGTCGGT750.002662678210.12663711
GTCCTAC10100.010.0770531
TATGGCG12100.010.04193216
GATATAC1909.640644E-1110.0126151
GGCGAGG5700.09.991878519
TATAAGG2002.5465852E-119.9873312
CTGTAGG13050.09.9857621
TATACAG2151.0913936E-119.7309775
Produced by FastQC (version 0.11.5)