FastQCFastQC Report
Thu 26 May 2016
SRR1512898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036439
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129410.4261900206129614No Hit
TATCAACGCAGAGTACTTTTTTTTT74870.2465717243125912No Hit
GGTATCAACGCAGAGTACTTTTTTT70350.23168586624002654No Hit
ACGCAGAGTACTTTTTTTTTTTTTT34050.11213793525903205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.013.8682561
TAGGACC6450.011.3391764
GTAGGAC13850.011.0414623
TGTAGGA15900.010.9918612
GTATCAA56050.010.9046881
GGACCGT1803.6379788E-1110.5539536
GTCCTAC14950.010.5074311
TAAGACG750.002652515410.1316284
TTAGGAC8650.010.1023453
TATCGCG1701.5661499E-910.0572967
ATACGAC856.607014E-410.0571313
GTGTAAG4500.09.9434971
CTGTAGG17000.09.9123851
GACGTGA6650.09.8556467
TCCTACA18050.09.7878262
TAGGACG13150.09.751214
TCGCGCG1951.6370905E-109.742119
ATTTCTA10400.09.5906815
TACCGTA900.00111570489.4985587
TACGACT800.0045238179.4984024
Produced by FastQC (version 0.11.5)