##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512898_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3036439 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.624307947566212 31.0 30.0 33.0 20.0 34.0 2 28.835121337856613 31.0 30.0 34.0 16.0 34.0 3 28.934915537575428 31.0 30.0 34.0 20.0 34.0 4 32.38829760782285 35.0 33.0 37.0 22.0 37.0 5 32.536566023555885 35.0 33.0 37.0 25.0 37.0 6 32.42794141426849 36.0 33.0 37.0 23.0 37.0 7 32.34484802757441 36.0 33.0 37.0 22.0 37.0 8 32.24032855591698 36.0 33.0 37.0 21.0 37.0 9 33.527222183617056 38.0 34.0 39.0 18.0 39.0 10 33.424157705786286 38.0 33.0 39.0 17.0 39.0 11 33.42929233882189 38.0 34.0 39.0 17.0 39.0 12 33.33036296793711 38.0 33.0 39.0 17.0 39.0 13 33.27946222532381 38.0 33.0 39.0 17.0 39.0 14 34.15909194948425 39.0 33.0 41.0 17.0 41.0 15 34.2881273096545 39.0 34.0 41.0 17.0 41.0 16 34.21524226240013 39.0 34.0 41.0 17.0 41.0 17 34.00534540624725 39.0 33.0 41.0 15.0 41.0 18 33.977367897066266 39.0 33.0 41.0 11.0 41.0 19 34.08961747626084 39.0 34.0 41.0 12.0 41.0 20 34.06719845187076 39.0 34.0 41.0 10.0 41.0 21 33.99327666388161 39.0 34.0 41.0 10.0 41.0 22 33.9669876457258 39.0 34.0 41.0 8.0 41.0 23 33.639790886627395 39.0 33.0 41.0 2.0 41.0 24 33.69531184390663 39.0 34.0 41.0 2.0 41.0 25 33.42018067874902 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4031612557140889 1101 2 0.04180353395875258 1101 3 0.37821956839671245 1101 4 0.49166759145549577 1101 5 0.4281655107284905 1101 6 0.3400993400993144 1101 7 0.04368584890511329 1101 8 0.27227281186394947 1101 9 0.27819227668405944 1101 10 0.42346007936546926 1101 11 0.4346143908547475 1101 12 0.3031371241791234 1101 13 0.6583685581157255 1101 14 0.6542705209653974 1101 15 0.5039019007384269 1101 16 0.418611515349248 1101 17 -0.30152657627885304 1101 18 0.20740856746776615 1101 19 -0.4224976554437987 1101 20 -1.0915040372967937 1101 21 0.09663490407850617 1101 22 0.18308021729874469 1101 23 -0.3064627614177553 1101 24 0.1864960605257977 1101 25 -0.10437986900987539 1102 1 0.6181440270344822 1102 2 0.6044667017543546 1102 3 0.4894549078814059 1102 4 0.657609665609975 1102 5 0.5489953978742079 1102 6 0.39151132641821107 1102 7 0.3027532130136308 1102 8 0.4758888512526056 1102 9 0.741990984342145 1102 10 0.7218874434214229 1102 11 0.4276293781676017 1102 12 0.5791414173671754 1102 13 0.5605940253867345 1102 14 0.7255621951978668 1102 15 0.6717684196779032 1102 16 0.5905214378975927 1102 17 0.6898604800499015 1102 18 0.7723119933211251 1102 19 0.6012118278814143 1102 20 0.7985615308110852 1102 21 0.8686365689248632 1102 22 0.7263883964395674 1102 23 0.5779309793446785 1102 24 -0.04936446884311607 1102 25 0.37837202872361786 1103 1 0.5224493991282984 1103 2 0.6229984109585338 1103 3 0.7457561187622943 1103 4 0.826155265568282 1103 5 0.679151964512748 1103 6 0.32611221278061464 1103 7 0.1834134347445442 1103 8 0.4349048727241467 1103 9 0.5210952411667549 1103 10 0.717642004018586 1103 11 0.5003511040687485 1103 12 0.49640856683609513 1103 13 0.6969327192549599 1103 14 0.7115913437327208 1103 15 0.7435684649781962 1103 16 0.6762664774150835 1103 17 0.3342960203360974 1103 18 0.6577702378745727 1103 19 0.6525306878373556 1103 20 0.8580694012362073 1103 21 0.6436941864901513 1103 22 0.6944067490722929 1103 23 0.06071494304030267 1103 24 0.12088859143233321 1103 25 0.43453268842419845 1104 1 0.8390114430185847 1104 2 0.7652029416525039 1104 3 0.751768830723929 1104 4 0.9766241875057489 1104 5 0.6912801057150872 1104 6 0.6512115630160125 1104 7 0.8474936930940657 1104 8 0.3658599458862639 1104 9 0.8580195290744328 1104 10 0.8897416525491124 1104 11 0.5776057912429522 1104 12 0.8405847185895041 1104 13 0.8333217120649508 1104 14 1.174362176255471 1104 15 0.9223972525531607 1104 16 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2215 9 -3.2207025911590215 2215 10 -1.5006951780049285 2215 11 -1.1350429673399631 2215 12 -1.112957559635305 2215 13 -1.1809298905475956 2215 14 -0.7079035702519292 2215 15 -0.9581112642578304 2215 16 -1.0412525305189604 2215 17 -1.2099744403348893 2215 18 -1.0286033768841776 2215 19 -0.9946855488099899 2215 20 -0.7671117399521279 2215 21 -1.5128340422162552 2215 22 -1.3468507041747344 2215 23 -1.2823380907615416 2215 24 -1.141096827201757 2215 25 -2.1133474530237812 2216 1 -1.3381043073550565 2216 2 -1.2245213668627528 2216 3 -2.726635565488685 2216 4 -3.1682941097353634 2216 5 -2.731161145046592 2216 6 -1.3085612265661908 2216 7 -2.8195126676287927 2216 8 -1.0595687217996996 2216 9 -1.4270443746612997 2216 10 -0.7126263652447591 2216 11 -0.6540528783251816 2216 12 -1.147836032785385 2216 13 -0.6719051932512983 2216 14 -1.5177332010692126 2216 15 -1.1587240945002932 2216 16 -1.2350244650316355 2216 17 -1.0750894350795122 2216 18 -0.5869677377176785 2216 19 -1.2396602814390434 2216 20 -1.5369010498648947 2216 21 -1.2673486177184827 2216 22 -0.8284613431927852 2216 23 -0.756723330578879 2216 24 -1.0844486285782509 2216 25 -1.0341026237789563 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 165067.0 3 3.0 4 1395.0 5 8918.0 6 11015.0 7 10382.0 8 9358.0 9 8420.0 10 7325.0 11 6953.0 12 6681.0 13 6310.0 14 6107.0 15 6329.0 16 6571.0 17 6995.0 18 7835.0 19 9554.0 20 12009.0 21 15474.0 22 19456.0 23 23186.0 24 27296.0 25 30731.0 26 33698.0 27 36867.0 28 41469.0 29 48943.0 30 59296.0 31 73092.0 32 91569.0 33 116234.0 34 150452.0 35 197297.0 36 266784.0 37 410059.0 38 1097309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.103288485443656 17.869843092964594 12.780062503758618 24.246805917833132 2 15.622176718523098 19.63371900957104 40.240087822066116 24.504016449839746 3 19.473034848417978 24.676583520465535 28.53574461049385 27.31463702062264 4 12.787900346494869 15.709194629988273 37.02554576235458 34.47735926116227 5 13.901276906334731 38.0463609750442 33.86812239961424 14.184239719006827 6 32.90369149637442 35.270632827949896 17.687112722478577 14.1385629531971 7 29.247013068340998 31.709925676900514 20.709085216130255 18.333976038628236 8 26.86329654998258 34.96302928745047 19.751878040989045 18.421796121577913 9 27.55870864224306 14.954169898181332 19.195147668546632 38.29197379102898 10 16.789936586147313 28.094993542585645 32.14688096008797 22.968188911179073 11 35.18925466606238 22.591471303201352 23.077714144452226 19.141559886284043 12 24.209882981521908 24.514049271840342 29.74342170759028 21.532646039047478 13 29.25712952226383 21.432404264562116 25.436677264568047 23.873788948606002 14 22.464614129033915 21.178124870213903 26.574919293647188 29.782341707104994 15 24.217772504037708 29.04093740729754 23.170737334783613 23.570552753881145 16 23.46457435198312 27.580588648089726 25.709028809430183 23.245808190496973 17 22.69018675506644 27.665113341324126 26.41328871149157 23.23141119211786 18 22.638701072132402 26.777596316205543 28.02441753362735 22.5592850780347 19 24.03871035371424 26.41179295801994 27.109740978313933 22.439755709951882 20 24.44553225014257 26.04671631016307 27.297857682050875 22.209893757643485 21 24.41493960289426 25.776540102300494 26.682933405141657 23.12558688966359 22 24.499662260281447 26.20893182272967 26.75096368729357 22.540442229695316 23 23.809150217348236 26.10410497588517 27.029899350971498 23.05684545579509 24 23.850485391449556 25.828606764528367 27.314233237752646 23.006674606269428 25 23.67838915714954 26.001527487020148 27.21788049803856 23.10220285779175 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 481.0 1 481.0 2 1090.5 3 1700.0 4 1700.0 5 1700.0 6 3194.5 7 4689.0 8 4689.0 9 4689.0 10 5685.5 11 6682.0 12 6682.0 13 6682.0 14 9257.0 15 11832.0 16 11832.0 17 11832.0 18 20708.5 19 29585.0 20 29585.0 21 29585.0 22 45275.5 23 60966.0 24 60966.0 25 60966.0 26 89133.5 27 117301.0 28 117301.0 29 117301.0 30 149845.5 31 182390.0 32 182390.0 33 182390.0 34 209561.5 35 236733.0 36 236733.0 37 236733.0 38 264174.0 39 291615.0 40 291615.0 41 291615.0 42 317209.0 43 342803.0 44 342803.0 45 342803.0 46 370068.0 47 397333.0 48 397333.0 49 397333.0 50 395827.5 51 394322.0 52 394322.0 53 394322.0 54 365113.5 55 335905.0 56 335905.0 57 335905.0 58 297773.0 59 259641.0 60 259641.0 61 259641.0 62 219396.5 63 179152.0 64 179152.0 65 179152.0 66 139963.0 67 100774.0 68 100774.0 69 100774.0 70 75438.5 71 50103.0 72 50103.0 73 50103.0 74 35572.5 75 21042.0 76 21042.0 77 21042.0 78 14534.0 79 8026.0 80 8026.0 81 8026.0 82 5250.0 83 2474.0 84 2474.0 85 2474.0 86 1576.0 87 678.0 88 678.0 89 678.0 90 419.5 91 161.0 92 161.0 93 161.0 94 96.5 95 32.0 96 32.0 97 32.0 98 25.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3313420753718418 2 0.00987999429594996 3 0.008595595037476466 4 0.010505727268026791 5 0.011559593326261454 6 0.08032435362607317 7 0.07867768791008152 8 0.10182980787692426 9 0.08286021882870033 10 0.0894798150069868 11 0.08444101791605232 12 0.0955395448418361 13 0.08249795237118217 14 0.08565955054588616 15 0.0836176850580565 16 0.08582421711748532 17 0.09705447730054845 18 0.1002819421038921 19 0.09326714615376762 20 0.09250967992441146 21 0.08022555368311367 22 0.10005140890365326 23 0.09313541289648829 24 0.0889858152921893 25 0.08885408203490996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3036439.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.68860927552971 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36070488607288 36.67863496872062 2 13.929058477842593 14.120892055187356 3 5.424204235219362 8.24836107329123 4 2.6576692445194507 5.388542316761546 5 1.5158956523905838 3.841932121325025 6 0.9880178420695634 3.0048750212349673 7 0.6392555069337456 2.268208083273671 8 0.45818837448493976 1.8579945191085758 9 0.33810724050098806 1.5424367226283922 >10 1.564483866598346 13.679222093958238 >50 0.0852001626889135 2.939642382075205 >100 0.0350347387454673 3.3331560170028647 >500 0.002612357458525023 0.9005002205572727 >1k 0.0013714876654042669 1.2864619904686767 >5k 1.3061787289564446E-4 0.48073907067718596 >10k+ 6.530893644782223E-5 0.42840134372906374 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12941 0.4261900206129614 No Hit TATCAACGCAGAGTACTTTTTTTTT 7487 0.2465717243125912 No Hit GGTATCAACGCAGAGTACTTTTTTT 7035 0.23168586624002654 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3405 0.11213793525903205 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.29333143198332E-5 0.0 9 0.0 0.0 0.0 3.29333143198332E-5 0.0 10 0.0 0.0 0.0 3.29333143198332E-5 0.0 11 0.0 0.0 0.0 3.29333143198332E-5 0.0 12 0.0 0.0 0.0 3.29333143198332E-5 3.29333143198332E-5 13 0.0 0.0 0.0 3.29333143198332E-5 1.317332572793328E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2540 0.0 13.868256 1 TAGGACC 645 0.0 11.339176 4 GTAGGAC 1385 0.0 11.041462 3 TGTAGGA 1590 0.0 10.991861 2 GTATCAA 5605 0.0 10.904688 1 GGACCGT 180 3.6379788E-11 10.553953 6 GTCCTAC 1495 0.0 10.507431 1 TAAGACG 75 0.0026525154 10.131628 4 TTAGGAC 865 0.0 10.102345 3 TATCGCG 170 1.5661499E-9 10.057296 7 ATACGAC 85 6.607014E-4 10.057131 3 GTGTAAG 450 0.0 9.943497 1 CTGTAGG 1700 0.0 9.912385 1 GACGTGA 665 0.0 9.855646 7 TCCTACA 1805 0.0 9.787826 2 TAGGACG 1315 0.0 9.75121 4 TCGCGCG 195 1.6370905E-10 9.74211 9 ATTTCTA 1040 0.0 9.59068 15 TACCGTA 90 0.0011157048 9.498558 7 TACGACT 80 0.004523817 9.498402 4 >>END_MODULE